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Showing: 1 - 20 of 23 results
  1. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...to have slower, but larger, calcium changes. The GCaMP6 family of sensors are commonly used calcium reporters...the plasma membrane(GCaMP-X is a nucleus-targeted GCaMP6 with an extra apoCaM-binding motif to prevent aberrant...expression in both zebrafish and mammalian systems. The GCaMP6, GCaMP7, and GCaMP8 sensors are also suitable for...optimized for neuron-specific, astrocyte-specific (GCaMP6 only) or mammalian cell expression, and compatible...specific to the neuronal cell body or the axon (GCaMP6 only). The genetic elements required for Drosophila...
  2. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    ...AAV1 pAAV-hSynapsin1-axon-GCaMP6s 112005  AAV9 pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3) 112010  AAV9...AAV9 pAAV-hSynapsin1-FLEx-axon-GCaMP6s New voltage sensors Plasmid Serotype Name 119036  AAV1  ...variant 1513-WPRE 105714  AAV8  pAAV-Ef1a-fDIO-GCaMP6s Optogenetics Plasmid Serotype Name 124651...
  3. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...axon-GCaMP6s-P2A-mKate2 (AAV9) pAAV-hSynapsin1-GCaMP6s-P2A-mKate2 (AAV9) pAAV-hSynapsin1-GCaMP6s-P2A-mRuby3...dLight1.2 and voltage sensor Ace2N and additional GCaMP6 constructs: pAAV-CAG-dLight1.2 (AAV1) pGP-AAV-...-mRuby3 (AAV9) pAAV-CAG-SomaGCaMP6f2 (AAV9) Plus, additional AAV serotypes of the most recent jGCaMP8...
  4. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...viral prep in serotype AAV1. Synapse targeting GCaMP6s from the Rylan Larsen lab are now available in ...ready-to-use AAV5 viral vector. Synaptophysin-tagged GCaMP6s is enriched in the pre-synaptic terminal providing...expressing GCaMP6f with this promoter. Image calcium transients in PV interneurons with AAV-S5E2-GCaMP6f are ...viral preps from recent months: Axon targeting GCaMP6f from Lin Tian’s lab is available in serotype AAV1...
  5. Biosensor AAV Preps

    Type
    Collection
    ...GCaMP8 axon-GCaMP8 soma-GCaMP8 GCaMP7 GCaMP6 axon-GCaMP6 soma-GCaMP6 Twitch2B Red Calcium Sensors CaMPARI...pAAV-Ef1a-fDIO-GCaMP6s EF1a GCaMP6s none Flp dependent 8 Larsen 105715 pAAV-Ef1a-DIO-Synaptophysin-GCaMP6s EF1a ...EF1a GCaMP6s none Cre dependent 5 Larsen 51084 AAV-hSyn1-GCaMP6s-P2A-nls-dTomato Syn GCaMP6s dTomato Constitutive...pAAV-hSynapsin1-axon-GCaMP6s Syn axon-GCaMP6s none Constitutive 1 Tian 112000 pAAV-hSynapsin1-axon-GCaMP6f Syn axon-GCaMP6f...axon-GCaMP6f none Constitutive 1 Tian 112005 pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3 Syn axon-GCaMP6s bicistronic...-hSyn1-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA Syn GCaMP6s mRuby2 Constitutive 1 Bonhoeffer , Huebener , Rose 51082... 51082 AAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato EF1a GCaMP6s dTomato Cre dependent 1 Ting 105714 pAAV-Ef1a-fDIO-GCaMP6s...
  6. Brain Initiative Collection

    Type
    Collection
    ...111262-AAV1 pAAV-hSynapsin1-axon-GCaMP6s For enriched expression of GCaMP6s in axons Lin Tian 111568-AAV5...AAV9 pAAV-hSynapsin1-axon-GCaMP6s-P2A-mKate2 For enriched expression of GCaMP6s in axons with cytosolic ...AAV5 pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3 For enriched expression of GCaMP6s in axons with cytosolic ...AAV9 pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3 For enriched expression of GCaMP6s in axons with cytosolic ...112006-AAV9 pAAV-hSynapsin1-GCaMP6s-P2A-mKate2 For co-expression of GCaMP6s with mKate2 Lin Tian 112007...112007-AAV9 pAAV-hSynapsin1-GCaMP6s-P2A-mRuby3 For co-expression of GCaMP6s with mRuby3 Lin Tian 112010-AAV5...pAAV-hSynapsin1-FLEx-axon-GCaMP6s For cre-dependent enriched expression of GCaMP6s in axons Lin Tian 112010...
  7. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...cytoplasmic-tdTomato and Synaptophysin-GFP. Synapse targeting GCaMP6s from the Rylan Larsen lab, now available in a ready...calcium transients in PV interneurons with AAV-S5E2-GCaMP6f, now available as a ready-to-use viral prep in ...
  8. Retrograde AAV viral preps

    Type
    Collection
    ...Calcium sensor Fishell 51084 AAV-hSyn1-GCaMP6s-P2A-nls-dTomato Syn GCaMP6s and physically separate nuclear dTomato...GCaMP8f GCaMP8s GCaMP8m GCaMP7f GCaMP7s GCaMP7b GCaMP6f GCaMP6s GECO Other Biosensors Glutamate Norepinephrine... Calcium Sensors 51085 AAV-hSyn1-GCaMP6f-P2A-nls-dTomato Syn GCaMP6f and physically separate nuclear dTomato...Calcium sensor Ting 51083 AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato EF1a GCaMP6f and physically separate nuclear ...Calcium sensor Ting 83899 pAAV-mDlx-GCaMP6f-Fishell-2 mDlx GCaMP6f expression under the control of the...
  9. Five Popular Model Organisms

    Type
    Blog Post
    ...expresses a genetically encoded calcium sensor, GCaMP6 (Nguyen et al., 2015). If you are interested in...
  10. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ... of GCaMP6s or GCaMP7b sensors fused to synaptophysin (Cre or Flp-dependent) Larsen Lab AAV GCaMP6 Plasmids...Bicistronic vector expressing GCaMP6 and mRuby2 for imaging neural activity (GCaMP6f/m/s) (Constitutive or Cre-dependent...Calcium GCaMP6f/s Ca sensors and separate nuclear localized dTomato fluorophore Ting Lab GCaMP6 Plasmids...Dimidschstein Calcium Enriched expression of GCaMP6s or GCaMP6f in axons (Constitutive or Cre-dependent) ...1280. Lin Tian Calcium Plasma membrane targeted GCaMP6s calcium sensor A polarized Ca(2+), diacylglycerol...sensor in astrocytes (GCaMP6f), membrane-tethered Khakh Lab pZac2.1 gfaABC1D-lck-GCaMP6f Plasmid Baljit Khakh...Calcium Soma-targeted expression of GCaMP7f or GCaMP6f Precision Calcium Imaging of Dense Neural Populations...
  11. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...AV-1-50942 50942-AAV1 pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA Tobias Bonhoeffer, Mark Huebener, Tobias...-68717 68717-AAV1 pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA Tobias Bonhoeffer, Mark Huebener, Tobias...68720 68720-AAV1 pAAV-hSyn1-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA Tobias Bonhoeffer, Mark Huebener, Tobias...gfaABC1D-lck-GCaMP6f Baljit Khakh AV-5-PV3435 100834-AAV5 pENN.AAV.CamKII.GCaMP6f.WPRE.SV40 James M. Wilson...AV-1-50943 50943-AAV1 pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA Tobias Bonhoeffer, Mark Huebener, Tobias...-68719 68719-AAV1 pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA Tobias Bonhoeffer, Mark Huebener, Tobias...Loren Looger AV-1-PV2816 100835-AAV1 pAAV.CAG.Flex.GCaMP6f.WPRE.SV40 GENIE, Douglas Kim AV-1-PV2817 100839...
  12. Hot Plasmids - August 2020

    Type
    Blog Post
    ...mDlx-GFP, hDlx-FLEX-GFP, hDlx-Flex-dTomato, mDlx-GCaMP6f, hDlx-GiDREADD-dTomato and mDlx-ChR2-mCherry. ...
  13. Deisseroth INTRSECT Collection

    Type
    Collection
    ...137119 pAAV-EF1a-Con/Fon-GCaMP6M Cre AND Flp 137120 pAAV-Ef1a-Con/Foff 2.0-GCaMP6M Cre AND NOT Flp FRT/F5...137121 pAAV-Ef1a-Coff/Fon-GCaMP6M Flp AND NOT Cre 137122 pAAV-Ef1a-Con/Fon-GCaMP6F Cre AND Flp 137123 pAAV-Ef1a-Con...-Ef1a-Con/Foff 2.0-GCaMP6F Cre AND NOT Flp FRT/F5 137124 pAAV-Ef1a-Coff/Fon-GCaMP6F Flp AND NOT Cre 137125...AND Flp AND Vcre 137165 pAAV-Ef1a-Con/Fon/VCon-GCaMP6M Cre AND Flp AND Vcre References Fenno LE, Mattis...
Showing: 1 - 20 of 23 results