Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Showing: 1 - 15 of 15 results
  1. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...known as HaloTag is actually HaloTag7, and recent specialized variants run up to HaloTag11. HaloTag is now... (Gallagher et al., 2009). HaloTag: Scientists at Promega developed HaloTag in 2008 (Los et al., 2008)...159 Trimpethoprim  (TMP-tag) Few Easy HaloTag 295 Chloralkane (CA) Many Easy CLIP-tag...proteins (often up to 10-fold more). So, labeling a HaloTag or SNAP-tag construct can help you better detect...protein of interest. For example, the Cohen lab used HaloTag for their “ticker tape” method for intracellular...tags like tetracysteine (Jing & Cornish, 2011). HaloTag and SNAP-tag are the most popular self-labeling...both 182 aa), and eDHFR (159 aa) are smaller than HaloTag (295 aa) – for comparison, common fluorescent proteins...
  2. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...Nabet et al., Chemical Biology, 2018.    HaloTag The HaloTag was initially developed as an affinity tag...straightforward system – HaloTag and its PROTAC are all that is needed to facilitate degradation. HaloTag is also the...proteolysis-targeting chimera (PROTAC) was developed for HaloTag. PROTACs are engineered molecules which target ...targeted to the proteasome for degradation. The HaloTag is quite large (33 kDa) and does not self-cleave...involves imaging at some stage, then choosing a HaloTag could pay dividends down the road. It is difficult...
  3. Hot Plasmids - November 2023

    Type
    Blog Post
    ...externally-timed bands of color with HaloTag-iPAK4 by applying different HaloTag dyes and showed they could record.... B) HEK cell with an iPAK4 fiber labeled with HaloTag-iPAK4. Scale bar 10 µm. C) Images of iPAK4 fibers...fibers in cultured rat hippocampal neurons. HaloTag dyes are switched from yellow to red at 0 h and back...
  4. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...interchangeable labeling using the HaloTag® system, the new Janelia Fluor HaloTag Ligands expand the self-labeling... self-labeling proteins would be SNAP-tag® and HaloTag®. These consist of a genetically encoded enzyme...2017). These dyes were adapted into ligands for HaloTag® and are currently offered at Promega. Due to the...
  5. Hot Plasmids - February 2022

    Type
    Blog Post
    ...SNAP-tag, and HaloTag fusions, while the anti-HA frankenbodies include mEGFP, mCherry, and HaloTag. ...
  6. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...chemigenetic calcium indicator for fluorescence using HaloTag ligands Bright and tunable far-red chemigenetic...chemigenetic calcium indicator for fluorescence using HaloTag ligands A modular chemigenetic calcium indicator...
  7. Luciferase Plasmids

    Type
    Collection
    ...wavelengths : NanoLuc® with either SNAP‐tag or HaloTag7 tag in which labeling of the self‐labeling tag...
Showing: 1 - 15 of 15 results