...reporter like GFP, some smaller CRISPR-Cas proteins such as SaCas9, CRISPR gRNAs, or your gene of interest...: FLEX Cre-Lox system.  WPRE The Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element...this awesome experiment you want to do, but it requires some AAV. You’ve never worked with AAV before,...

...Nanoblades to Existing CRISPR Delivery Methods When compared head-to-head with existing CRISPR delivery technologies...delivery Learn about CRISPR delivery methods for mammalian cells Browse our CRISPR 101 blog post Resources...Addgene.org Check out our comprehensive CRISPR guide Find CRISPR plasmids and resources Learn about viral...microinjecting zygotes with CRISPR components. However, this sometimes requires intracellular injections ...CRISPR is a simple and versatile tool for genome engineering, but its utility is dependent on its ability...ability to infiltrate cells. Options for CRISPR delivery include plasmid transfection, RNP electroporation...pluripotent stem cells (hiPSCs). Nanoblades, a new CRISPR delivery method developed by the Ricci Lab and ...