Overcoming the AAV Size Limitation for CRISPR Delivery
Type
Blog Post
Published
Sept. 16, 2020, 1 p.m.
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Learn more about Cas9 Variants and the Expanded CRISPR Toolbox
Read about CRISPR enzyme Cpf1
Read our...one AAV, Cas9 must be made smaller. Previous attempts to “shrink” Cas9 include the use of St1Cas9 (~3.3 ...toxicity.
More AAV-based CRISPR systems
Smaller Cas proteins
SaCas9 isn't the only CRISPR enzyme that’s small...amino acids in length, Cas9 from Campylobacter jejuni (CjCas9) is the smallest Cas9 ortholog characterized...used CjCas9 with AAV to target genes in mouse muscle and eye tissue.
Neisseria meningitidis Cas9 (NmeCas9...vehicle for Cas9. However, to allow for more room for regulatory sequences while still fitting Cas9 and its...equally potent Cas9 protein, the Zhang lab took a different approach. They analyzed over 600 Cas9 orthologs...