LOCUS       pCTcon2                 6456 bp    DNA     circular 

FEATURES             Location/Qualifiers
     CDS             715..1575
                     /gene="AmpR"
                     /codon_start="1"
     CDS             complement(5315..5989)
                     /gene="TRP1"
                     /codon_start="1"
     misc_feature    2940..3606
                     /gene="GAL1"
                     /codon_start="1"
     misc_feature    4763..5191
                     /gene="F1 ORI"
     misc_feature    4256..4562
                     /gene="MFa1 Terminator"
     CDS             3968..4207
                     /gene="CD20"
                     /codon_start="1"
     CDS             3701..3904
                     /gene="Aga2p"
                     /codon_start="1"
     misc_feature    615..714
                     /gene="AmpR Promoter (bla)"
     CDS             3644..3700
                     /gene="Leader"
                     /codon_start="1"
     CDS             4214..4243
                     /gene="cMyc Tag"
                     /codon_start="1"
     CDS             3935..3961
                     /gene="HA"
                     /codon_start="1"
     CDS             3905..3922
                     /gene="Linker"
                     /codon_start="1"
     CDS             3923..3937
                     /gene="Factor Xa Cleavage Site"
                     /codon_start="1"
BASE COUNT     1811 a   1493 c   1308 g   1844 t
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttaggacgg atcgcttgcc tgtaacttac acgcgcctcg tatcttttaa tgatggaata
      121 atttgggaat ttactctgtg tttatttatt tttatgtttt gtatttggat tttagaaagt
      181 aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa aataaacaaa ggtttaaaaa
      241 atttcaacaa aaagcgtact ttacatatat atttattaga caagaaaagc agattaaata
      301 gatatacatt cgattaacga taagtaaaat gtaaaatcac aggattttcg tgtgtggtct
      361 tctacacaga caagatgaaa caattcggca ttaatacctg agagcaggaa gagcaagata
      421 aaaggtagta tttgttggcg atccccctag agtcttttac atcttcggaa aacaaaaact
      481 attttttctt taatttcttt ttttactttc tatttttaat ttatatattt atattaaaaa
      541 atttaaatta taattatttt tatagcacgt gatgaaaagg acccaggtgg cacttttcgg
      601 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
      661 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
      721 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
      781 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
      841 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
      901 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
      961 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     1021 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     1081 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     1141 ccgaaggagc taaccgcttt ttttcacaac atgggggatc atgtaactcg ccttgatcgt
     1201 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     1261 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     1321 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     1381 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     1441 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     1501 ggcagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     1561 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     1621 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     1681 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     1741 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     1801 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     1861 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     1921 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     1981 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2041 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2101 acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc
     2161 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2221 agggagcttc caggggggaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2281 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc cgagcctatg gaaaaacgcc
     2341 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     2401 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     2461 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     2521 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     2581 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttacctcact
     2641 cattaggcac cccaggcttt acactttatg cttccggctc ctatgttgtg tggaattgtg
     2701 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gctcggaatt
     2761 aaccctcact aaagggaaca aaagctgggt acccgacagg ttatcagcaa caacacagtc
     2821 atatccattc tcaattagct ctaccacagt gtgtgaacca atgtatccag caccacctgt
     2881 aaccaaaaca attttagaag tactttcact ttgtaactga gctgtcattt atattgaatt
     2941 ttcaaaaatt cttacttttt ttttggatgg acgcaaagaa gtttaataat catattacat
     3001 ggcattacca ccatatacat atccatatac atatccatat ctaatcttac ttatatgttg
     3061 tggaaatgta aagagcccca ttatcttagc ctaaaaaaac cttctctttg gaactttcag
     3121 taatacgctt aactgctcat tgctatattg aagtacggat tagaagccgc cgagcgggtg
     3181 acagccctcc gaaggaagac tctcctccgt gcgtcctcgt cttcaccggt cgcgttcctg
     3241 aaacgcagat gtgcctcgcg ccgcactgct ccgaacaata aagattctac aatactagct
     3301 tttatggtta tgaagaggaa aaattggcag taacctggcc ccacaaacct tcaaatgaac
     3361 gaatcaaatt aacaaccata ggatgataat gcgattagtt ttttagcctt atttctgggg
     3421 taattaatca gcgaagcgat gatttttgat ctattaacag atatataaat gcaaaaactg
     3481 cataaccact ttaactaata ctttcaacat tttcggtttg tattacttct tattcaaatg
     3541 taataaaagt atcaacaaaa aattgttaat atacctctat actttaacgt caaggagaaa
     3601 aaaccccgga tcgaattccc tacttcatac attttcaatt aagatgcagt tacttcgctg
     3661 tttttcaata ttttctgtta ttgcttcagt tttagcacag gaactgacaa ctatatgcga
     3721 gcaaatcccc tcaccaactt tagaatcgac gccgtactct ttgtcaacga ctactatttt
     3781 ggccaacggg aaggcaatgc aaggagtttt tgaatattac aaatcagtaa cgtttgtcag
     3841 taattgcggt tctcacccct caacaactag caaaggcagc cccataaaca cacagtatgt
     3901 ttttaaggac aatagctcga cgattgaagg tagataccca tacgacgttc cagactacgc
     3961 tctgcaggct agtggtggag gaggctctgg tggaggcggt agcggaggcg gagggtcggc
     4021 tagctgcggt ggcggcggta ctagcaaaat ttctcatttt ttgaaaatgg aatctttgaa
     4081 ttttattaga gctcatactc catatattaa tatttacaat tgtgaaccag ctaatccatc
     4141 tgaaaaaaat tctccatcta ctcaatattg ttattctatt caatcttccc aggtcgactg
     4201 cgggggcgga tccgaacaaa agcttatttc tgaagaggac ttgtaatagc tcgagatctg
     4261 ataacaacag tgtagatgta acaaaatcga ctttgttccc actgtacttt tagctcgtac
     4321 aaaatacaat atacttttca tttctccgta aacaacatgt tttcccatgt aatatccttt
     4381 tctatttttc gttccgttac caactttaca catactttat atagctattc acttctatac
     4441 actaaaaaac taagacaatt ttaattttgc tgcctgccat atttcaattt gttataaatt
     4501 cctataattt atcctattag tagctaaaaa aagatgaatg tgaatcgaat cctaagagaa
     4561 ttgagctcca attcgcccta tagtgagtcg tattacaatt cactggccgt cgttttacaa
     4621 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatcccccc
     4681 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     4741 agcctgaatg gcgaatggcg cgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     4801 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     4861 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     4921 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     4981 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     5041 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     5101 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     5161 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttcc
     5221 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcagg caagtgcaca
     5281 aacaatactt aaataaatac tactcagtaa taacctattt cttagcattt ttgacgaaat
     5341 ttgctatttt gttagagtct tttacaccat ttgtctccac acctccgctt acatcaacac
     5401 caataacgcc atttaatcta agcgcatcac caacattttc tggcgtcagt ccaccagcta
     5461 acataaaatg taagctttcg gggctctctt gccttccaac ccagtcagaa atcgagttcc
     5521 aatccaaaag ttcacctgtc ccacctgctt ctgaatcaaa caagggaata aacgaatgag
     5581 gtttctgtga agctgcactg agtagtatgt tgcagtcttt tggaaatacg agtcttttaa
     5641 taactggcaa accgaggaac tcttggtatt cttgccacga ctcatctcca tgcagttgga
     5701 cgatatcaat gccgtaatca ttgaccagag ccaaaacatc ctccttaggt tgattacgaa
     5761 acacgccaac caagtatttc ggagtgcctg aactattttt atatgctttt acaagacttg
     5821 aaattttcct tgcaataacc gggtcaattg ttctctttct attgggcaca catataatac
     5881 ccagcaagtc agcatcggaa tctagagcac attctgcggc ctctgtgctc tgcaagccgc
     5941 aaactttcac caatggacca gaactacctg tgaaattaat aacagacata ctccaagctg
     6001 cctttgtgtg cttaatcacg tatactcacg tgctcaatag tcaccaatgc cctccctctt
     6061 ggccctctcc ttttcttttt tcgaccgaat taattcttaa tcggcaaaaa aagaaaagct
     6121 ccggatcaag attgtacgta aggtgacaag ctatttttca ataaagaata tcttccacta
     6181 ctgccatctg gcgtcataac tgcaaagtac acatatatta cgatgctgtc tattaaatgc
     6241 ttcctatatt atatatatag taatgtcgtt tatggtgcac tctcagtaca atctgctctg
     6301 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     6361 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     6421 gtcagaggtt ttcaccgtca tcaccgaaac gcgcga                          
//
