LOCUS       RCIscript-GoldyT        4651 bp    DNA     circular     27-JUN-2012
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|629852342|
COMMENT     VNTDBDATE|629978779|
COMMENT     LSOWNER|
COMMENT     VNTNAME|RCIscript-GoldyTALEN|
COMMENT     VNTAUTHORNAME|Dan Carlson|
COMMENT     VNTAUTHOREML|carl0246@umn.edu|
FEATURES             Location/Qualifiers
     misc_feature    1345..1932
                     /vntifkey="21"
                     /label=FokI\Homodimer\CDS
     misc_feature    270..272
                     /vntifkey="21"
                     /label=start\codon
     primer_bind     556..575
                     /vntifkey="28"
                     /label=TAL_F1
     misc_feature    complement(768..1043)
                     /vntifkey="21"
                     /label=lacZ'
     promoter        complement(1044..1165)
                     /vntifkey="30"
                     /label=lacZ\promoter
     misc_feature    710..746
                     /vntifkey="21"
                     /label=left
     misc_feature    1188..1228
                     /vntifkey="21"
                     /label=right
     primer_bind     complement(1193..1212)
                     /vntifkey="28"
                     /label=TAL_rev2
     CDS             2976..3833
                     /vntifkey="4"
                     /label=Amp
                     /note="Amp resistance gene"
     rep_origin      2389..2845
                     /vntifkey="33"
                     /label=F1\Ori
     promoter        complement(2206..2226)
                     /vntifkey="30"
                     /label=T7
     5'UTR           173..216
                     /vntifkey="52"
                     /label=5'UTR
                     /note="Xenopus beta-globin 5' UTR"
     3'UTR           1957..2127
                     /vntifkey="50"
                     /label=3'\UTR
                     /note="Xenopus beta-globin 3' UTR"
     promoter        146..165
                     /vntifkey="30"
                     /label=T3\Promoter
     rep_origin      3897..126
                     /vntifkey="33"
                     /label=ColE1\Ori
BASE COUNT     1261 a      1114 c      1175 g      1101 t 
ORIGIN
        1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg 
       61 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc 
      121 ggaagagcgc ccaatacgca aaccgaatta accctcacta aagggaagct tgcttgttct 
      181 ttttgcagaa gctcagaata aacgctcaac tttggcagat ctaactcgag aaagatattg 
      241 tatatatcgt aacaatagga ggttcaacaa tggcttcctc ccctccaaag aaaaagagaa 
      301 aggttagttg gaaggacgca agtggttggt ctagagtgga tctacgcacg ctcggctaca 
      361 gtcagcagca gcaagagaag atcaaaccga aggtgcgttc gacagtggcg cagcaccacg 
      421 aggcactggt gggccatggg tttacacacg cgcacatcgt tgcgctcagc caacacccgg 
      481 cagcgttagg gaccgtcgct gtcacgtatc agcacataat cacggcgttg ccagaggcga 
      541 cacacgaaga catcgttggc gtcggcaaac agtggtccgg cgcacgcgcc ctggaggcct 
      601 tgctcacgga tgcgggggag ttgagaggtc cgccgttaca gttggacaca ggccaacttg 
      661 tgaagattgc aaaacgtggc ggcgtgaccg caatggaggc agtgcatgca tcgcgcaatg 
      721 cactgacggg tgcccccctg gagacgggcg ccgctacagg gcgcgtccca ttcgccattc 
      781 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg 
      841 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca 
      901 cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg 
      961 ggtaccgggc cccccctcga ggtcctccag cttttgttcc ctttagtgag ggttaattgc 
     1021 gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat 
     1081 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag 
     1141 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc caccggtcgt ctccaacgac 
     1201 cacctcgtcg ccttggcctg cctcggcgga cgtcctgcca tggatgcagt gaaaaaggga 
     1261 ttgccgcacg cgccggaatt gatcagaaga gtcaatcgcc gtattggcga acgcacgtcc 
     1321 catcgcgttg cctctagatc ccagctagtg aaatctgaat tggaagagaa gaaatctgaa 
     1381 cttagacata aattgaaata tgtgccacat gaatatattg aattgattga aatcgcaaga 
     1441 aattcaactc aggatagaat ccttgaaatg aaggtgatgg agttctttat gaaggtttat 
     1501 ggttatcgtg gtaaacattt gggtggatca aggaaaccag acggagcaat ttatactgtc 
     1561 ggatctccta ttgattacgg tgtgatcgtt gatactaagg catattcagg aggttataat 
     1621 cttccaattg gtcaagcaga tgaaatgcaa agatatgtcg aagagaatca aacaagaaac 
     1681 aagcatatca accctaatga atggtggaaa gtctatccat cttcagtaac agaatttaag 
     1741 ttcttgtttg tgagtggtca tttcaaagga aactacaaag ctcagcttac aagattgaat 
     1801 catatcacta attgtaatgg agctgttctt agtgtagaag agcttttgat tggtggagaa 
     1861 atgattaaag ctggtacatt gacacttgag gaagtgagaa ggaaatttaa taacggtgag 
     1921 ataaactttt aataggctag tgactgacta ggatctggtt accactaaac cagcctcaag 
     1981 aacacccgaa tggagtctct aagctacata ataccaactt acacttacaa aatgttgtcc 
     2041 cccaaaatgt agccattcgt atctgctcct aataaaaaga aagtttcttc acattctaaa 
     2101 aaaaaaaaaa aaaaaaaaaa aaaaaaaccc cccccccccc cccccccccc cccccccgca 
     2161 tgcctgcagg tcgactagga tccccgggta ccgagctcca attcgcccta tagtgagtcg 
     2221 tattacaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc 
     2281 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc 
     2341 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggaa attgtaagcg 
     2401 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat 
     2461 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg 
     2521 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc 
     2581 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt 
     2641 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag 
     2701 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg 
     2761 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc 
     2821 ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc 
     2881 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct 
     2941 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg 
     3001 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg 
     3061 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc 
     3121 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca 
     3181 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac 
     3241 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa 
     3301 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg 
     3361 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt 
     3421 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg 
     3481 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc 
     3541 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga 
     3601 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta 
     3661 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc 
     3721 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg 
     3781 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt 
     3841 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa 
     3901 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt 
     3961 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt 
     4021 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt 
     4081 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga 
     4141 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag 
     4201 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata 
     4261 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg 
     4321 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga 
     4381 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 
     4441 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa 
     4501 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt 
     4561 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac 
     4621 ggttcctggc cttttgctgg ccttttgctc a 
//
