LOCUS       pSicoR-Ef1a-mCh-Puro        8141 bp    DNA     circular     23-JUN-2011
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|593689317|
COMMENT     VNTDBDATE|593689444|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pSicoR-Ef1a-mCh-Puro|
COMMENT     VNTAUTHORNAME|Matthew Spindler|
COMMENT     VNTAUTHORTEL|415-734-2833|
COMMENT     VNTAUTHORFAX|415-355-0960|
COMMENT     VNTAUTHOREML|mspindler@gladstone.ucsf.edu|
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FEATURES             Location/Qualifiers
     misc_feature    1076..1213
                     /vntifkey="21"
                     /label=Psi
     misc_feature    1707..1910
                     /vntifkey="21"
                     /label=RRE
     misc_feature    2944..2958
                     /vntifkey="21"
                     /label=shRNA\Cloning\Site
     promoter        2631..2943
                     /vntifkey="29"
                     /label=U6\Promoter
     misc_feature    2825..2858
                     /vntifkey="21"
                     /label=loxP
     misc_feature    4941..4974
                     /vntifkey="21"
                     /label=loxP
     misc_feature    5030..5409
                     /vntifkey="21"
                     /label=WRE
     primer          2481..2500
                     /vntifkey="27"
                     /label=Lox\Replace\For\Primer
     primer          complement(493..512)
                     /vntifkey="27"
                     /label=Lox\Replace\Rev\Primer
     misc_marker     3554..4261
                     /vntifkey="22"
                     /label=mCherry
     promoter        2989..3531
                     /vntifkey="29"
                     /label=EF-1alpha\promoter
     misc_feature    4325..4918
                     /vntifkey="21"
                     /label=Puro
     misc_feature    4268..4321
                     /vntifkey="21"
                     /label=T2A
     misc_feature    complement(7151..8008)
                     /vntifkey="21"
                     /label=AmpR
BASE COUNT     2020 a      2102 c      2157 g      1862 t 
ORIGIN
        1 gcttaagcgg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa 
       61 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg 
      121 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca 
      181 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata 
      241 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc 
      301 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac 
      361 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa 
      421 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag 
      481 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc 
      541 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct 
      601 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg 
      661 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt 
      721 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga 
      781 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt 
      841 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac 
      901 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg 
      961 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct 
     1021 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac 
     1081 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta 
     1141 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta 
     1201 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga 
     1261 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta 
     1321 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat 
     1381 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt 
     1441 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag 
     1501 agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgcgctgat cttcagacct 
     1561 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa 
     1621 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa 
     1681 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg 
     1741 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag 
     1801 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc 
     1861 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa 
     1921 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg 
     1981 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag 
     2041 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa 
     2101 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg 
     2161 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga 
     2221 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag 
     2281 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc 
     2341 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac 
     2401 ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa 
     2461 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac 
     2521 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta 
     2581 ttacagggac agcagagatc cagtttggtt agtaccgggc ccgctctaga gatccgacgc 
     2641 gccatctcta ggcccgcgcc ggccccctcg cacagacttg tgggagaagc tcggctactc 
     2701 ccctgccccg gttaatttgc atataatatt tcctagtaac tatagaggct taatgtgcga 
     2761 taaaagacag ataatctgtt ctttttaata ctagctacat tttacatgat aggcttggat 
     2821 ttctataact tcgtatagca tacattatac gaagttatac atgtcacaaa aggaaactca 
     2881 ccctaactgt aaagtaattg tgtgttttga gactataaat atcccttgga gaaaagcctt 
     2941 gttaacgcgc ggtgaccctc gagtactagg atccattagg cggccgcgga tctgcgatcg 
     3001 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg 
     3061 aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga 
     3121 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt 
     3181 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg 
     3241 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg 
     3301 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta 
     3361 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc 
     3421 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt 
     3481 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagcgct 
     3541 accggtcgcc accatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt 
     3601 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg 
     3661 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg 
     3721 tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc 
     3781 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt 
     3841 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc 
     3901 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc 
     3961 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta 
     4021 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg 
     4081 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg 
     4141 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt 
     4201 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa 
     4261 gccccgggag ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc 
     4321 tcgaaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt 
     4381 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga 
     4441 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga 
     4501 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga 
     4561 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg 
     4621 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga 
     4681 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg 
     4741 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct 
     4801 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc 
     4861 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgccta 
     4921 agaattcgtc gagggaccta ataacttcgt atagcataca ttatacgaag ttatacatgt 
     4981 ttaagggttc cggttccact aggtacaatt cgatatcaag cttatcgata atcaacctct 
     5041 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct 
     5101 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat 
     5161 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt 
     5221 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat 
     5281 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc 
     5341 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga 
     5401 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc 
     5461 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga 
     5521 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc 
     5581 tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc gacctcgatc 
     5641 gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt 
     5701 gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac 
     5761 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg 
     5821 ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct 
     5881 accacacaca aggctacttc cctgattggc agaactacac accagggcca gggatcagat 
     5941 atccactgac ctttggatgg tgctacaagc tagtaccagt tgagcaagag aaggtagaag 
     6001 aagccaatga aggagagaac acccgcttgt tacaccctgt gagcctgcat gggatggatg 
     6061 acccggagag agaagtatta gagtggaggt ttgacagccg cctagcattt catcacatgg 
     6121 cccgagagct gcatccggac tgtactgggt ctctctggtt agaccagatc tgagcctggg 
     6181 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc 
     6241 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct 
     6301 tttagtcagt gtggaaaatc tctagcagca tgtgagcaaa aggccagcaa aaggccagga 
     6361 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 
     6421 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 
     6481 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 
     6541 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 
     6601 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 
     6661 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 
     6721 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 
     6781 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg 
     6841 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 
     6901 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 
     6961 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 
     7021 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 
     7081 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 
     7141 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 
     7201 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 
     7261 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 
     7321 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 
     7381 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 
     7441 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 
     7501 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 
     7561 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 
     7621 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 
     7681 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 
     7741 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 
     7801 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 
     7861 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 
     7921 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 
     7981 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 
     8041 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 
     8101 taggggttcc gcgcacattt ccccgaaaag tgccacctga c 
//
