LOCUS       pRNA-U6-ZipmiR12        5125 bp    DNA     circular     10-FEB-2012
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|617469809|
COMMENT     VNTDBDATE|617469857|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pRNA-U6-ZipmiR122|
COMMENT     VNTAUTHORNAME|JunXie|
COMMENT     VNTAUTHORTEL|508-856-3316|
COMMENT     VNTAUTHORFAX|508-856-1552|
COMMENT     VNTAUTHOREML|jun.xie@umassmed.edu|
COMMENT     VNTAUTHORAD1|University of Massachusetts Medical School|
COMMENT     VNTAUTHORAD2|381 Plantation St., Biotech 5, Ste. 250|
COMMENT     VNTAUTHORAD3|Worcester, MA 01605|
COMMENT     VNTOAUTHORNAME|Demo User|
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FEATURES             Location/Qualifiers
     promoter        4875..77
                     /vntifkey="29"
                     /label=U6\Promoter
     promoter        896..1241
                     /vntifkey="30"
                     /label=SV40\Promoter
     exon            1282..2076
                     /vntifkey="61"
                     /label=Neomycin
     rep_origin      2790..3430
                     /vntifkey="33"
                     /label=pUC\ori
     CDS             3578..4438
                     /vntifkey="4"
                     /label=Ampicillin
     misc_feature    80..143
                     /vntifkey="21"
                     /label=miR-122\Zip
BASE COUNT     1202 a      1309 c      1309 g      1305 t 
ORIGIN
        1 aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg gtttatatat 
       61 cttgtggaaa ggacgcgggg atcctggtca gtgacaatgt ttgcttcctg tcagacaaac 
      121 accattgtca cactccattt ttaagcttaa gtttaaaccg ctgatcagcc tcgactgtgc 
      181 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag 
      241 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta 
      301 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag 
      361 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca 
      421 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg 
      481 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg 
      541 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg 
      601 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt 
      661 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt 
      721 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta 
      781 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa 
      841 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg 
      901 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta 
      961 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat 
     1021 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac 
     1081 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga 
     1141 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg 
     1201 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga 
     1261 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc 
     1321 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga 
     1381 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct 
     1441 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac 
     1501 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct 
     1561 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt 
     1621 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt 
     1681 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt 
     1741 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag 
     1801 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt 
     1861 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg 
     1921 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg 
     1981 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg 
     2041 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg 
     2101 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat 
     2161 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg 
     2221 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac 
     2281 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 
     2341 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc 
     2401 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca 
     2461 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg 
     2521 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg 
     2581 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc 
     2641 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 
     2701 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 
     2761 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 
     2821 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 
     2881 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 
     2941 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 
     3001 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 
     3061 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 
     3121 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 
     3181 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 
     3241 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt 
     3301 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 
     3361 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 
     3421 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 
     3481 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 
     3541 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 
     3601 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 
     3661 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 
     3721 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 
     3781 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 
     3841 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc 
     3901 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 
     3961 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 
     4021 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 
     4081 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 
     4141 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg 
     4201 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 
     4261 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 
     4321 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 
     4381 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 
     4441 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 
     4501 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 
     4561 gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta 
     4621 caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg 
     4681 tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt 
     4741 gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat 
     4801 atacgcgttt aatacgactc actataggga gagagagaga attaccctca ctaaagggag 
     4861 gagaagcatg aattccccag tggaaagacg cgcaggcaaa acgcaccacg tgacggagcg 
     4921 tgaccgcgcg ccgagcgcgc gccaaggtcg ggcaggaaga gggcctattt cccatgattc 
     4981 cttcatattt gcatatacga tacaaggctg ttagagagat aattagaatt aatttgactg 
     5041 taaacacaaa gatattagta caaaatacgt gacgtagaaa gtaataattt cttgggtagt 
     5101 ttgcagtttt aaaattatgt tttaa 
//
