ID pSicoR-Ef1a-mCh standard; circular DNA; ; 7484 BP. DT 12-JAN-2006 CC This file is created by Vector NTI CC http://www.invitrogen.com/ CC ORIGDB|GenBank CC VNTDATE|593687056| CC VNTDBDATE|593687170| CC LSOWNER| CC VNTAUTHORNAME|Matthew Spindler| CC VNTAUTHORTEL|415-734-2833| CC VNTAUTHORFAX|415-355-0960| CC VNTAUTHOREML|mspindler@gladstone.ucsf.edu| FH Key Location/Qualifiers FH FT misc_feature 1076..1213 FT /vntifkey="21" FT /label=Psi FT misc_feature 1707..1910 FT /vntifkey="21" FT /label=RRE FT misc_feature 2944..2958 FT /vntifkey="21" FT /label=shRNA\Cloning\Site FT promoter 2631..2943 FT /vntifkey="29" FT /label=U6\Promoter FT misc_feature 2825..2858 FT /vntifkey="21" FT /label=loxP FT misc_feature 4284..4317 FT /vntifkey="21" FT /label=loxP FT misc_feature 4373..4752 FT /vntifkey="21" FT /label=WRE FT primer 2481..2500 FT /vntifkey="27" FT /label=Lox\Replace\For\Primer FT primer complement(493..512) FT /vntifkey="27" FT /label=Lox\Replace\Rev\Primer FT misc_marker 3554..4264 FT /vntifkey="22" FT /label=mCherry FT promoter 2989..3531 FT /vntifkey="29" FT /label=EF-1alpha\promoter FT misc_feature complement(6494..7351) FT /vntifkey="21" FT /label=AmpR SQ Sequence 7484 BP; 1921 A; 1851 C; 1932 G; 1780 t; gcttaagcgg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa 60 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg 120 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca 180 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata 240 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc 300 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac 360 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa 420 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag 480 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc 540 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct 600 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg 660 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt 720 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga 780 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt 840 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac 900 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg 960 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct 1020 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac 1080 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta 1140 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta 1200 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga 1260 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta 1320 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat 1380 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt 1440 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag 1500 agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgcgctgat cttcagacct 1560 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa 1620 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa 1680 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg 1740 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag 1800 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc 1860 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa 1920 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg 1980 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag 2040 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa 2100 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg 2160 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga 2220 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag 2280 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc 2340 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac 2400 ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa 2460 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac 2520 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta 2580 ttacagggac agcagagatc cagtttggtt agtaccgggc ccgctctaga gatccgacgc 2640 gccatctcta ggcccgcgcc ggccccctcg cacagacttg tgggagaagc tcggctactc 2700 ccctgccccg gttaatttgc atataatatt tcctagtaac tatagaggct taatgtgcga 2760 taaaagacag ataatctgtt ctttttaata ctagctacat tttacatgat aggcttggat 2820 ttctataact tcgtatagca tacattatac gaagttatac atgtcacaaa aggaaactca 2880 ccctaactgt aaagtaattg tgtgttttga gactataaat atcccttgga gaaaagcctt 2940 gttaacgcgc ggtgaccctc gagtactagg atccattagg cggccgcgga tctgcgatcg 3000 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg 3060 aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga 3120 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt 3180 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg 3240 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg 3300 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta 3360 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc 3420 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt 3480 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagcgct 3540 accggtcgcc accatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt 3600 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg 3660 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg 3720 tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc 3780 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt 3840 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc 3900 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc 3960 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta 4020 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg 4080 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg 4140 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt 4200 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa 4260 gtaagaattc gtcgagggac ctaataactt cgtatagcat acattatacg aagttataca 4320 tgtttaaggg ttccggttcc actaggtaca attcgatatc aagcttatcg ataatcaacc 4380 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac 4440 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt 4500 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt 4560 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg 4620 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac 4680 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac 4740 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt 4800 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc 4860 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg 4920 ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg 4980 atcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg 5040 attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg 5100 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa 5160 aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga 5220 tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg ccagggatca 5280 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa gagaaggtag 5340 aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg catgggatgg 5400 atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca tttcatcaca 5460 tggcccgaga gctgcatccg gactgtactg ggtctctctg gttagaccag atctgagcct 5520 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag 5580 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac 5640 ccttttagtc agtgtggaaa atctctagca gcatgtgagc aaaaggccag caaaaggcca 5700 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 5760 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 5820 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 5880 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 5940 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 6000 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 6060 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 6120 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat 6180 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 6240 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 6300 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 6360 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 6420 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 6480 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 6540 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 6600 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 6660 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 6720 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 6780 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 6840 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 6900 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 6960 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 7020 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 7080 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 7140 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 7200 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 7260 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 7320 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 7380 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 7440 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgac 7484 //