ID pSicoR-Ef1a-mCh-Puro standard; circular DNA; ; 8141 BP. DT 12-JAN-2006 CC This file is created by Vector NTI CC http://www.invitrogen.com/ CC ORIGDB|GenBank CC VNTDATE|593689317| CC VNTDBDATE|593689444| CC LSOWNER| CC VNTAUTHORNAME|Matthew Spindler| CC VNTAUTHORTEL|415-734-2833| CC VNTAUTHORFAX|415-355-0960| CC VNTAUTHOREML|mspindler@gladstone.ucsf.edu| FH Key Location/Qualifiers FH FT misc_feature 1076..1213 FT /vntifkey="21" FT /label=Psi FT misc_feature 1707..1910 FT /vntifkey="21" FT /label=RRE FT misc_feature 2944..2958 FT /vntifkey="21" FT /label=shRNA\Cloning\Site FT promoter 2631..2943 FT /vntifkey="29" FT /label=U6\Promoter FT misc_feature 2825..2858 FT /vntifkey="21" FT /label=loxP FT misc_feature 4941..4974 FT /vntifkey="21" FT /label=loxP FT misc_feature 5030..5409 FT /vntifkey="21" FT /label=WRE FT primer 2481..2500 FT /vntifkey="27" FT /label=Lox\Replace\For\Primer FT primer complement(493..512) FT /vntifkey="27" FT /label=Lox\Replace\Rev\Primer FT misc_marker 3554..4261 FT /vntifkey="22" FT /label=mCherry FT promoter 2989..3531 FT /vntifkey="29" FT /label=EF-1alpha\promoter FT misc_feature 4325..4918 FT /vntifkey="21" FT /label=Puro FT misc_feature 4268..4321 FT /vntifkey="21" FT /label=T2A FT misc_feature complement(7151..8008) FT /vntifkey="21" FT /label=AmpR SQ Sequence 8141 BP; 2020 A; 2102 C; 2157 G; 1862 t; gcttaagcgg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa 60 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg 120 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca 180 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata 240 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc 300 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac 360 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa 420 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag 480 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc 540 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct 600 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg 660 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt 720 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga 780 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt 840 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac 900 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg 960 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct 1020 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac 1080 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta 1140 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta 1200 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga 1260 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta 1320 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat 1380 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt 1440 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag 1500 agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgcgctgat cttcagacct 1560 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa 1620 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa 1680 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg 1740 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag 1800 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc 1860 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa 1920 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg 1980 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag 2040 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa 2100 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg 2160 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga 2220 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag 2280 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc 2340 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac 2400 ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa 2460 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac 2520 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta 2580 ttacagggac agcagagatc cagtttggtt agtaccgggc ccgctctaga gatccgacgc 2640 gccatctcta ggcccgcgcc ggccccctcg cacagacttg tgggagaagc tcggctactc 2700 ccctgccccg gttaatttgc atataatatt tcctagtaac tatagaggct taatgtgcga 2760 taaaagacag ataatctgtt ctttttaata ctagctacat tttacatgat aggcttggat 2820 ttctataact tcgtatagca tacattatac gaagttatac atgtcacaaa aggaaactca 2880 ccctaactgt aaagtaattg tgtgttttga gactataaat atcccttgga gaaaagcctt 2940 gttaacgcgc ggtgaccctc gagtactagg atccattagg cggccgcgga tctgcgatcg 3000 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg 3060 aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga 3120 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt 3180 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg 3240 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg 3300 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta 3360 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc 3420 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt 3480 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagcgct 3540 accggtcgcc accatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt 3600 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg 3660 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg 3720 tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc 3780 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt 3840 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc 3900 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc 3960 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta 4020 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg 4080 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg 4140 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt 4200 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa 4260 gccccgggag ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc 4320 tcgaaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt 4380 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga 4440 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga 4500 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga 4560 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg 4620 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga 4680 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg 4740 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct 4800 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc 4860 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgccta 4920 agaattcgtc gagggaccta ataacttcgt atagcataca ttatacgaag ttatacatgt 4980 ttaagggttc cggttccact aggtacaatt cgatatcaag cttatcgata atcaacctct 5040 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct 5100 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat 5160 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt 5220 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat 5280 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc 5340 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga 5400 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc 5460 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga 5520 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc 5580 tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc gacctcgatc 5640 gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt 5700 gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac 5760 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg 5820 ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct 5880 accacacaca aggctacttc cctgattggc agaactacac accagggcca gggatcagat 5940 atccactgac ctttggatgg tgctacaagc tagtaccagt tgagcaagag aaggtagaag 6000 aagccaatga aggagagaac acccgcttgt tacaccctgt gagcctgcat gggatggatg 6060 acccggagag agaagtatta gagtggaggt ttgacagccg cctagcattt catcacatgg 6120 cccgagagct gcatccggac tgtactgggt ctctctggtt agaccagatc tgagcctggg 6180 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc 6240 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct 6300 tttagtcagt gtggaaaatc tctagcagca tgtgagcaaa aggccagcaa aaggccagga 6360 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 6420 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 6480 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 6540 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 6600 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 6660 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 6720 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 6780 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg 6840 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 6900 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 6960 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 7020 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 7080 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 7140 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 7200 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 7260 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 7320 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 7380 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 7440 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 7500 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 7560 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 7620 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 7680 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 7740 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 7800 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 7860 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 7920 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 7980 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 8040 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 8100 taggggttcc gcgcacattt ccccgaaaag tgccacctga c 8141 //