LOCUS pBCN41-R4R3 9097 bp DNA circular UNA 05-sep-2008 DEFINITION pDESTR4R3, Ampicillin/Chloramphenicol, Needs ccdB resistant strain KEYWORDS . SOURCE pCG150 Sequence sequence (unknown) ORGANISM pCG150 Sequence sequence other sequences; artificial sequences; vectors. COMMENT See Addgene pCG150 Sequence sequence at www.addgene.org FEATURES Location/Qualifiers Promoter 165..1127 /label=Pmyo-2 CDS join(1128..1298,1350..1503,1555..1718,1770..1997) /label="worm GFP" 3'_UTR 2028..2392 /label="myo-2 3pUTR" Promoter 2402..2978 /label=Prpl-28 CDS join(3016..3214,3266..3465,3517..3717) /label="Puromycin Resistance" Intron 3215..3265 /label=SynIVS.R Intron 3466..3516 /label=SynIVS.T Operon 3727..3908 /label="C. elegans outron (rpl16)" PolyA_signal 3777..3782 /label=polyA CDS 3909..4703 /label=NeoR 3'_UTR 4727..5109 /label=let-858_3pflank Misc._feature 5105..5139 /label="SpeI-XcmI-BglII-AvrII-SacI sites for linearisation" Promoter 5459..5487 /label="AmpR_promoter" CDS 5529..6389 /gene="Ampicillin" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" /label="Ampicillin" Replication_ori 6544..7163 /label="pBR322_origin" Misc._recombina 7352..7452 /label="attR4" CDS complement(7493..7798) /label="ccdB" CDS complement(8143..8801) /gene="CAT/CamR" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A*" /label="CAT/CamR" Misc._recombina complement(8910..9010) /label="attR3" ORIGIN 1 TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA 61 TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG CGCCTGATGC GGTATTTTCT 121 CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATGCCGG GCCCcatttt atatctgagt 181 agtatccttt gctttaaatg tccataaaac taattttata atcaataaaa caacgtttgt 241 aaatcaactg agtttacaag tagagacatt gagggatact ttcactatgc taaagtgata 301 atcgaccaaa taataacttc actttggtat ttattcctgt cttataaatg ttatgtatga 361 attaaattca tatgcatatg gctcactctg acaattttta ataatcttcc agatcaatat 421 tgactaccga tgcgggtggt cttttgcttt gaattctgct gaactttaca ccccgaacag 481 caatgtgtgc ttcagcctaa aaaaaagtaa gtgtgttaat cagtgccccc gattcttcat 541 tttttgcccc tctctcccgt ttcgtcggca aaagaagaga aaataaagat aagtctcaag 601 ataggttggt aatcgctaaa gtggttgtgt ggataagagt agcaaaatgg caggaagagc 661 actttgcgcg cacacactgt actcattgtt ctggataaaa ttctctcgtt gtttgccgtc 721 ggatgtctgc ctctctgcat tgagccggct tcttcactat ctttagttaa cctaaaatgc 781 cgtttctttt ctcgtatccc cactatcccg ttgaggttct ctgctctctt cgctccctac 841 cgccagcgag caactatccg tgggggcgcc ttgctcggaa gatggggggg aagaaagaag 901 atttttgcta tttgcacttg agaaagagac ttttcctgcg tcgatggtta gagaacagtg 961 tgcagacact tttcagctac ctagaattac aattggatat ccccgcctcc caatccaccc 1021 acccagggaa aaagaagggc tcgccgaaaa tcaaagttat ctccaggctc gcgcatccca 1081 ccgagcggtt gacttctctc caccactttt cattttTacc ctcgaCcATG AGTAAAGGAG 1141 AAGAACTTTT CACTGGAGTT GTCCCAATTC TTGTTGAATT AGATGGTGAT GTTAATGGGC 1201 ACAAATTTTC TGTCAGTGGA GAGGGTGAAG GTGATGCAAC ATACGGAAAA CTTACCCTTA 1261 AATTTATTTG CACTACTGGA AAACTACCTG TTCCATGGGT AAGTTTAAAC ATATATATAC 1321 TAACTAACCC TGATTATTTA AATTTTCAGC CAACACTTGT CACTACTTTC TGTTATGGTG 1381 TTCAATGCTT CTCGAGATAC CCAGATCATA TGAAACGGCA TGACTTTTTC AAGAGTGCCA 1441 TGCCCGAAGG TTATGTACAG GAAAGAACTA TATTTTTCAA AGATGACGGG AACTACAAGA 1501 CACGTAAGTT TAAACAGTTC GGTACTAACT AACCATACAT ATTTAAATTT TCAGGTGCTG 1561 AAGTCAAGTT TGAAGGTGAT ACCCTTGTTA ATAGAATCGA GTTAAAAGGT ATTGATTTTA 1621 AAGAAGATGG AAACATTCTT GGACACAAAT TGGAATACAA CTATAACTCA CACAATGTAT 1681 ACATCATGGC AGACAAACAA AAGAATGGAA TCAAAGTTGT AAGTTTAAAC ATGATTTTAC 1741 TAACTAACTA ATCTGATTTA AATTTTCAGA ACTTCAAAAT TAGACACAAC ATTGAAGATG 1801 GAAGCGTTCA ACTAGCAGAC CATTATCAAC AAAATACTCC AATTGGCGAT GGCCCTGTCC 1861 TTTTACCAGA CAACCATTAC CTGTCCACAC AATCTGCCCT TTCGAAAGAT CCCAACGAAA 1921 AGAGAGACCA CATGGTCCTT CTTGAGTTTG TAACAGCTGC TGGGATTACA CATGGCATGG 1981 ATGAACTATA CAAATAGATC AACTGCTCTA ATTCCATCTC Gtttctctct tttctttcaa 2041 tctctttacc cgtttctctt cccctacgtc ccccaaacct accactgcac agtaacacat 2101 acacattcaa cgtgtcacaa taaaagccca tcaacacctt ctctgttgtt ttttttcttc 2161 aaatgcattt ttttcacttt gtttaataat ttttacgcat cggatttgta ggatagtgga 2221 aatttgtcct tcaatgcttc aattgattcg acacttctca atatttttga tcaataaacc 2281 attgattttc tatttagaag aagatcactg taaataacac caaaaacatc acagcaaaaa 2341 aactcccact caattatatt aCTGTTTTTA TTGAAACTAG AATAAATTTG AAGGGCCCCC 2401 CTCGAGGGGA GAAAATGCGG AGAGAAGAAC CCAGCATCTT AATAAAATTA TATAATTTTG 2461 AATAAAATCA AATTAAAAAT AAGATTAATT GAAACACAAC AATAGAATGC AGAAAATTAC 2521 TCATAAGAAA TAATTTATTT TGAAAGAAGT TGTGTCGATT TACGATAAAA ACTACACGCA 2581 AACGAGGCTC CGCGTTTACA CCGTTTGGCA ATGGAGCGCG CTTGCTATTA GATTTTAGGC 2641 CAGCTCAACG AAAGTTTAAT TTATTTTTCT TCTTTTTTTC CATTTTTTTG AGTAAAATAT 2701 CGAATTTTTT GTATTAATTC TGTAAATTTT ATAATTTGCA ACTATTTTGG CGTATTCTGC 2761 TTGTTTTTAT CAGCGAATTT TTATGCATTT CAGTCAAAAG TTTGAGCAGG AAAAATGACA 2821 TTTAGGACTT TGTAAAATTG TATTCTTAAA TTTGCTTGAA AACCTACGTG TACAATTGAT 2881 TTTATTGAAT TTTTCTTACG TTTCCCGTTG TATCTTATTG TATTTAACTT TCATCATAAA 2941 ATAATTTATT TCCAGGTAAA ATATCCGACG CTCTCGTGGA TCCCCGGGAT TGGCTCAGAA 3001 AAAAAGGTAC CGACCATGAC CGAGTACAAG CCCACGGTGC GCCTCGCCAC CCGCGACGAC 3061 GTCCCCAGGG CCGTACGCAC CCTCGCCGCC GCGTTCGCCG ACTACCCCGC CACGCGCCAC 3121 ACCGTCGATC CGGACCGCCA CATCGAGCGG GTCACCGAGC TGCAAGAACT CTTCCTCACG 3181 CGCGTCGGGC TCGACATCGG CAAGGTGTGG GTCGGTAAGT TTAAACATAT ATATACTAAC 3241 TAACCCTGAT TATTTAAATT TTCAGCGGAC GACGGCGCCG CGGTGGCGGT CTGGACCACG 3301 CCGGAGAGCG TCGAAGCGGG GGCGGTGTTC GCCGAGATCG GCCCGCGCAT GGCCGAGTTG 3361 AGCGGTTCCC GGCTGGCCGC GCAGCAACAG ATGGAAGGCC TCCTGGCGCC GCACCGGCCC 3421 AAGGAGCCCG CGTGGTTCCT GGCCACCGTC GGCGTCTCGC CCGACGTAAG TTTAAACATG 3481 ATTTTACTAA CTAACTAATC TGATTTAAAT TTTCAGCACC AGGGCAAGGG TCTGGGCAGC 3541 GCCGTCGTGC TCCCCGGAGT GGAGGCGGCC GAGCGCGCCG GGGTGCCCGC CTTCCTGGAG 3601 ACCTCCGCGC CCCGCAACCT CCCCTTCTAC GAGCGGCTCG GCTTCACCGT CACCGCCGAC 3661 GTCGAGgTGC CCGAAGGACC GCGCACCTGG TGCATGACCC GCAAGCCCGG TGCCTGATGA 3721 GCTAGCGATT ACTGAAATAT GTAAATATTA GAAGAATTTG TGCGGGATTC TTGCCAAATA 3781 AATTCTCGAA ATAGTTTTCA TATATTTATT GTTATGCGCG TTCTCTCGCT TCGATTTATC 3841 TCTCTTCCTT TACTCTCGTG TATTTCTAAA CTTTCCCCTT CTTATCAGTA TTTATTTCAG 3901 GGTAAACGat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga 3961 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc 4021 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga 4081 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg 4141 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc 4201 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg 4261 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga 4321 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc 4381 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca 4441 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg 4501 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct 4561 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg 4621 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc 4681 gccttcttga cgagttcttc tgaGCTAGCC GGCCATACAA GTAATCCGGA TGATCGACGC 4741 CAACGTCGTT GAATTTTCAA ATTTTAAATA CTGAATATTT GTTTTTTTTC CTATTATTTA 4801 TTTATTCTCT TTGTGTTTTT TTTCTTGCTT TCTAAAAAAT TAATTCAATC CAAATCTAAA 4861 CATTTTTTTT TCTCTTTCCG TCTCCCAATT CGTATTCCGC TCCTCTCATC TGAACACAAT 4921 GTGCAAGTTT ATTTATCTTC TCGCTTTCAT TTCATTAGGA CGTGGGGGGA ATTGGTGGAA 4981 GGGGGAAACA CACAAAAGGA TGATGGAAAT GAAATAAGGA CACACAATAT GCAACAACAT 5041 TCAATTCAGA AATATGGAGG AAGGTTTAAA AGAAAACATA AAAATATATA GAGGAGGAAG 5101 GAAAACTAGT CCAAGATCTC AGTGGTCCTA GGTGAGCTCC CATATGGTGC ACTCTCAGTG 5161 CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGCCCCGACA CCCGCCAACA CCCGCTGACG 5221 CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG 5281 GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGA CGAAAGGGCC 5341 TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG 5401 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 5461 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 5521 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 5581 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 5641 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 5701 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 5761 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 5821 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 5881 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 5941 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 6001 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 6061 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 6121 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 6181 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 6241 GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 6301 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 6361 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 6421 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 6481 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 6541 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 6601 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 6661 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTtCTT CTAGTGTAGC 6721 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 6781 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 6841 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 6901 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 6961 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 7021 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 7081 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 7141 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGTCTTTTGC TGGCCTTTGC 7201 TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA 7261 GTGAGCTGAT ACCAGCGGAT AACAATTTCA CACAGGAAAC AGCTATGACC ATGATTACGC 7321 CAAGCTATCA ACTTTGTATA GAAAAGTTGA ACGAGAAACG TAAAATGATA TAAATATCAA 7381 TATATTAAAT TAGATTTTGC ATAAAAAACA GACTACATAA TACTGTAAAA CACAACATAT 7441 CCAGTCACTA TGGTCGACCT GCAGACTGGC TGTGTATAAG GGAGCCTGAC ATTTATATTC 7501 CCCAGAACAT CAGGTTAATG GCGTTTTTGA TGTCATTTTC GCGGTGGCTG AGATCAGCCA 7561 CTTCTTCCCC GATAACGGAG ACCGGCACAC TGGCCATATC GGTGGTCATC ATGCGCCAGC 7621 TTTCATCCCC GATATGCACC ACCGGGTAAA GTTCACGGGa GACTTTATCT GACAGCAGAC 7681 GTGCACTGGC CAGGGGGATC ACCATCCGTC GCCCGGGCGT GTCAATAATA TCACTCTGTA 7741 CATCCACAAA CAGACGATAA CGGCTCTCTC TTTTATAGGT GTAAACCTTA AACTGCATTT 7801 CACCAGCCCC TGTTCTCGTC aGCAAAAGAG CCGTTCATTT CAATAAACCG GGCGACCTCA 7861 GCCATCCCTT CCTGATTTTC CGCTTTCCAG CGTTCGGCAC GCAGACGACG GGCTTCATTC 7921 TGCATGGTTG TGCTTACCag ACCGGAGATA TTGACATCAT ATATGCCTTG AGCAACTGAT 7981 AGCTGTCGCT GTCAACTGTC ACTGTAATAC GCTGCTTCAT AGCATACCTC TTTTTGACAT 8041 ACTTCGGGTA TACATATCAG TATATATTCT TATACCGCAA AAATCAGCGC GCAAATACGC 8101 ATACTGTTAT CTGGCTTTTA GTAAGCCGGA TCCacgcggc gtTTACGCCC CcCCTGCCAC 8161 TCATCGCAGT ACTGTTGTAA TTCATTAAGC ATTCTGCCGA CATGGAAGCC ATCACAAACG 8221 GCATGATGAA CCTGAATCGC CAGCGGCATC AGCACCTTGT CGCCTTGCGT ATAATATTTG 8281 CCCATGGTGA AAACGGGGGC GAAGAAGTTG TCCATATTGG CCACGTTTAA ATCAAAACTG 8341 GTGAAACTCA CCCAGGGATT GGCTGAGACG AAAAACATAT TCTCAATAAA CCCTTTAGGG 8401 AAATAGGCCA GGTTTTCACC GTAACACGCC ACATCTTGCG AATATATGTG TAGAAACTGC 8461 CGGAAATCGT CGTGGTATTC ACTCCAGAGC GATGAAAACG TTTCAGTTTG CTCATGGAAA 8521 ACGGTGTAAC AAGGGTGAAC ACTATCCCAT ATCACCAGCT CACCGTCTTT CATTGCCATA 8581 CGGAATTCCG GATGAGCATT CATCAGGCGG GCAAGAATGT GAATAAAGGC CGGATAAAAC 8641 TTGTGCTTAT TTTTCTTTAC GGTCTTTAAA AAGGCCGTAA TATCCAGCTG AACGGTCTGG 8701 TTATAGGTAC ATTGAGCAAC TGACTGAAAT GCCTCAAAAT GTTCTTTACG ATGCCATTGG 8761 GATATATCAA CGGTGGTATA TCCAGTGATT TTTTTCTCCA TTTTAGCTTC CTTAGCTCCT 8821 GAAAATCTCG ACGGATCCTA ACTCAAAATC CACACATTAT ACGAGCCGGA AGCATAAAGT 8881 GTAAAGCCTG GGGTGCCTAA TGCGGCCGCC ATAGTGACTG GATATGTTGT GTTTTACAGT 8941 ATTATGTAGT CTGTTTTTTA TGCAAAATCT AATTTAATAT ATTGATATTT ATATCATTTT 9001 ACGTTTCTCG TTCAACTTTA TTATACATAG TTGATAATTC ACTGGCCGTC GTTTTACAAC 9061 GTCGTGACTG GGAAAACCCT GGCGTTACCC AACTTAA //