LOCUS       pSicoR-Ef1a-mCh        7484 bp    DNA     circular     23-JUN-2011
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|593687056|
COMMENT     VNTDBDATE|593687170|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pSicoR-Ef1a-mCh|
COMMENT     VNTAUTHORNAME|Matthew Spindler|
COMMENT     VNTAUTHORTEL|415-734-2833|
COMMENT     VNTAUTHORFAX|415-355-0960|
COMMENT     VNTAUTHOREML|mspindler@gladstone.ucsf.edu|
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FEATURES             Location/Qualifiers
     misc_feature    1076..1213
                     /vntifkey="21"
                     /label=Psi
     misc_feature    1707..1910
                     /vntifkey="21"
                     /label=RRE
     misc_feature    2944..2958
                     /vntifkey="21"
                     /label=shRNA\Cloning\Site
     promoter        2631..2943
                     /vntifkey="29"
                     /label=U6\Promoter
     misc_feature    2825..2858
                     /vntifkey="21"
                     /label=loxP
     misc_feature    4284..4317
                     /vntifkey="21"
                     /label=loxP
     misc_feature    4373..4752
                     /vntifkey="21"
                     /label=WRE
     primer          2481..2500
                     /vntifkey="27"
                     /label=Lox\Replace\For\Primer
     primer          complement(493..512)
                     /vntifkey="27"
                     /label=Lox\Replace\Rev\Primer
     misc_marker     3554..4264
                     /vntifkey="22"
                     /label=mCherry
     promoter        2989..3531
                     /vntifkey="29"
                     /label=EF-1alpha\promoter
     misc_feature    complement(6494..7351)
                     /vntifkey="21"
                     /label=AmpR
BASE COUNT     1921 a      1851 c      1932 g      1780 t 
ORIGIN
        1 gcttaagcgg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa 
       61 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg 
      121 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca 
      181 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata 
      241 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc 
      301 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac 
      361 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa 
      421 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag 
      481 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc 
      541 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct 
      601 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg 
      661 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt 
      721 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga 
      781 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt 
      841 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac 
      901 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg 
      961 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct 
     1021 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac 
     1081 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta 
     1141 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta 
     1201 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga 
     1261 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta 
     1321 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat 
     1381 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt 
     1441 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag 
     1501 agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgcgctgat cttcagacct 
     1561 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa 
     1621 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa 
     1681 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg 
     1741 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag 
     1801 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc 
     1861 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa 
     1921 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg 
     1981 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag 
     2041 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa 
     2101 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg 
     2161 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga 
     2221 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag 
     2281 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc 
     2341 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac 
     2401 ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa 
     2461 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac 
     2521 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta 
     2581 ttacagggac agcagagatc cagtttggtt agtaccgggc ccgctctaga gatccgacgc 
     2641 gccatctcta ggcccgcgcc ggccccctcg cacagacttg tgggagaagc tcggctactc 
     2701 ccctgccccg gttaatttgc atataatatt tcctagtaac tatagaggct taatgtgcga 
     2761 taaaagacag ataatctgtt ctttttaata ctagctacat tttacatgat aggcttggat 
     2821 ttctataact tcgtatagca tacattatac gaagttatac atgtcacaaa aggaaactca 
     2881 ccctaactgt aaagtaattg tgtgttttga gactataaat atcccttgga gaaaagcctt 
     2941 gttaacgcgc ggtgaccctc gagtactagg atccattagg cggccgcgga tctgcgatcg 
     3001 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg 
     3061 aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga 
     3121 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt 
     3181 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg 
     3241 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg 
     3301 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta 
     3361 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc 
     3421 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt 
     3481 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagcgct 
     3541 accggtcgcc accatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt 
     3601 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg 
     3661 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg 
     3721 tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc 
     3781 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt 
     3841 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc 
     3901 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc 
     3961 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta 
     4021 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg 
     4081 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg 
     4141 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt 
     4201 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa 
     4261 gtaagaattc gtcgagggac ctaataactt cgtatagcat acattatacg aagttataca 
     4321 tgtttaaggg ttccggttcc actaggtaca attcgatatc aagcttatcg ataatcaacc 
     4381 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac 
     4441 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt 
     4501 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt 
     4561 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg 
     4621 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac 
     4681 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac 
     4741 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt 
     4801 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc 
     4861 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg 
     4921 ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg 
     4981 atcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg 
     5041 attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg 
     5101 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa 
     5161 aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga 
     5221 tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg ccagggatca 
     5281 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa gagaaggtag 
     5341 aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg catgggatgg 
     5401 atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca tttcatcaca 
     5461 tggcccgaga gctgcatccg gactgtactg ggtctctctg gttagaccag atctgagcct 
     5521 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag 
     5581 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac 
     5641 ccttttagtc agtgtggaaa atctctagca gcatgtgagc aaaaggccag caaaaggcca 
     5701 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 
     5761 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 
     5821 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 
     5881 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 
     5941 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 
     6001 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 
     6061 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 
     6121 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat 
     6181 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 
     6241 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 
     6301 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 
     6361 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 
     6421 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 
     6481 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 
     6541 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 
     6601 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 
     6661 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 
     6721 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 
     6781 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 
     6841 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 
     6901 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 
     6961 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 
     7021 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 
     7081 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 
     7141 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 
     7201 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 
     7261 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 
     7321 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 
     7381 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 
     7441 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgac 
//
