LOCUS       mMecp2-HA-SpCas9-short pA       7400 bp    DNA     circular     20-OCT-2014
FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /label=L-ITR
     misc_feature    401..430
                     /note="HA tag"
                     /label=HA tag
     misc_feature    431..463
                     /note="NLS"
                     /label=NLS
     misc_feature    464..4600
                     /note="SpCas9"
                     /label=SpCas9
     misc_feature    4565..4597
                     /note="NLS"
                     /label=NLS
     misc_feature    4601..4606
                     /note="EcoRI"
                     /label=EcoRI
     misc_feature    4663..4803
                     /label=R-ITR
     rep_origin      4895..5201
                     /label=f1 Origin
     misc_feature    5720..6577
                     /label=AmpR
     rep_origin      6728..7395
                     /label=pUC ori
     misc_feature    4607..4654
                     /note="s-polyA"
     promoter        160..388
                     /note="pMecp2 promoter"
ORIGIN      
          1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
         61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
        121 actccatcac taggggttcc tgcggcctct agaaagcttA GCTGAATGGG GTCCGCCTCT
        181 TTTCCCTGCC TAAACAGACA GGAACTCCTG CCAATTGAGG GCGTCACCGC TAAGGCTCCG
        241 CCCCAGCCTG GGCTCCACAA CCAATGAAGG GTAATCTCGA CAAAGAGCAA GGGGTGGGGC
        301 GCGGGCGCGC AGGTGCAGCA GCACACAGGC TGGTCGGGAG GGCGGGGCGC GACGTCTGCC
        361 GTGCGGGGTC CCGGCATCGG TTGCGCGCac cggtgccacc atgtacccat acgatgttcc
        421 agattacgct tcgccgaaga aaaagcgcaa ggtcgaagcg tccgacaaga agtacagcat
        481 cggcctggac atcggcacca actctgtggg ctgggccgtg atcaccgacg agtacaaggt
        541 gcccagcaag aaattcaagg tgctgggcaa caccgaccgg cacagcatca agaagaacct
        601 gatcggagcc ctgctgttcg acagcggcga aacagccgag gccacccggc tgaagagaac
        661 cgccagaaga agatacacca gacggaagaa ccggatctgc tatctgcaag agatcttcag
        721 caacgagatg gccaaggtgg acgacagctt cttccacaga ctggaagagt ccttcctggt
        781 ggaagaggat aagaagcacg agcggcaccc catcttcggc aacatcgtgg acgaggtggc
        841 ctaccacgag aagtacccca ccatctacca cctgagaaag aaactggtgg acagcaccga
        901 caaggccgac ctgcggctga tctatctggc cctggcccac atgatcaagt tccggggcca
        961 cttcctgatc gagggcgacc tgaaccccga caacagcgac gtggacaagc tgttcatcca
       1021 gctggtgcag acctacaacc agctgttcga ggaaaacccc atcaacgcca gcggcgtgga
       1081 cgccaaggcc atcctgtctg ccagactgag caagagcaga cggctggaaa atctgatcgc
       1141 ccagctgccc ggcgagaaga agaatggcct gttcggcaac ctgattgccc tgagcctggg
       1201 cctgaccccc aacttcaaga gcaacttcga cctggccgag gatgccaaac tgcagctgag
       1261 caaggacacc tacgacgacg acctggacaa cctgctggcc cagatcggcg accagtacgc
       1321 cgacctgttt ctggccgcca agaacctgtc cgacgccatc ctgctgagcg acatcctgag
       1381 agtgaacacc gagatcacca aggcccccct gagcgcctct atgatcaaga gatacgacga
       1441 gcaccaccag gacctgaccc tgctgaaagc tctcgtgcgg cagcagctgc ctgagaagta
       1501 caaagagatt ttcttcgacc agagcaagaa cggctacgcc ggctacattg acggcggagc
       1561 cagccaggaa gagttctaca agttcatcaa gcccatcctg gaaaagatgg acggcaccga
       1621 ggaactgctc gtgaagctga acagagagga cctgctgcgg aagcagcgga ccttcgacaa
       1681 cggcagcatc ccccaccaga tccacctggg agagctgcac gccattctgc ggcggcagga
       1741 agatttttac ccattcctga aggacaaccg ggaaaagatc gagaagatcc tgaccttccg
       1801 catcccctac tacgtgggcc ctctggccag gggaaacagc agattcgcct ggatgaccag
       1861 aaagagcgag gaaaccatca ccccctggaa cttcgaggaa gtggtggaca agggcgcttc
       1921 cgcccagagc ttcatcgagc ggatgaccaa cttcgataag aacctgccca acgagaaggt
       1981 gctgcccaag cacagcctgc tgtacgagta cttcaccgtg tataacgagc tgaccaaagt
       2041 gaaatacgtg accgagggaa tgagaaagcc cgccttcctg agcggcgagc agaaaaaggc
       2101 catcgtggac ctgctgttca agaccaaccg gaaagtgacc gtgaagcagc tgaaagagga
       2161 ctacttcaag aaaatcgagt gcttcgactc cgtggaaatc tccggcgtgg aagatcggtt
       2221 caacgcctcc ctgggcacat accacgatct gctgaaaatt atcaaggaca aggacttcct
       2281 ggacaatgag gaaaacgagg acattctgga agatatcgtg ctgaccctga cactgtttga
       2341 ggacagagag atgatcgagg aacggctgaa aacctatgcc cacctgttcg acgacaaagt
       2401 gatgaagcag ctgaagcggc ggagatacac cggctggggc aggctgagcc ggaagctgat
       2461 caacggcatc cgggacaagc agtccggcaa gacaatcctg gatttcctga agtccgacgg
       2521 cttcgccaac agaaacttca tgcagctgat ccacgacgac agcctgacct ttaaagagga
       2581 catccagaaa gcccaggtgt ccggccaggg cgatagcctg cacgagcaca ttgccaatct
       2641 ggccggcagc cccgccatta agaagggcat cctgcagaca gtgaaggtgg tggacgagct
       2701 cgtgaaagtg atgggccggc acaagcccga gaacatcgtg atcgaaatgg ccagagagaa
       2761 ccagaccacc cagaagggac agaagaacag ccgcgagaga atgaagcgga tcgaagaggg
       2821 catcaaagag ctgggcagcc agatcctgaa agaacacccc gtggaaaaca cccagctgca
       2881 gaacgagaag ctgtacctgt actacctgca gaatgggcgg gatatgtacg tggaccagga
       2941 actggacatc aaccggctgt ccgactacga tgtggaccat atcgtgcctc agagctttct
       3001 gaaggacgac tccatcgaca acaaggtgct gaccagaagc gacaagaacc ggggcaagag
       3061 cgacaacgtg ccctccgaag aggtcgtgaa gaagatgaag aactactggc ggcagctgct
       3121 gaacgccaag ctgattaccc agagaaagtt cgacaatctg accaaggccg agagaggcgg
       3181 cctgagcgaa ctggataagg ccggcttcat caagagacag ctggtggaaa cccggcagat
       3241 cacaaagcac gtggcacaga tcctggactc ccggatgaac actaagtacg acgagaatga
       3301 caagctgatc cgggaagtga aagtgatcac cctgaagtcc aagctggtgt ccgatttccg
       3361 gaaggatttc cagttttaca aagtgcgcga gatcaacaac taccaccacg cccacgacgc
       3421 ctacctgaac gccgtcgtgg gaaccgccct gatcaaaaag taccctaagc tggaaagcga
       3481 gttcgtgtac ggcgactaca aggtgtacga cgtgcggaag atgatcgcca agagcgagca
       3541 ggaaatcggc aaggctaccg ccaagtactt cttctacagc aacatcatga actttttcaa
       3601 gaccgagatt accctggcca acggcgagat ccggaagcgg cctctgatcg agacaaacgg
       3661 cgaaaccggg gagatcgtgt gggataaggg ccgggatttt gccaccgtgc ggaaagtgct
       3721 gagcatgccc caagtgaata tcgtgaaaaa gaccgaggtg cagacaggcg gcttcagcaa
       3781 agagtctatc ctgcccaaga ggaacagcga taagctgatc gccagaaaga aggactggga
       3841 ccctaagaag tacggcggct tcgacagccc caccgtggcc tattctgtgc tggtggtggc
       3901 caaagtggaa aagggcaagt ccaagaaact gaagagtgtg aaagagctgc tggggatcac
       3961 catcatggaa agaagcagct tcgagaagaa tcccatcgac tttctggaag ccaagggcta
       4021 caaagaagtg aaaaaggacc tgatcatcaa gctgcctaag tactccctgt tcgagctgga
       4081 aaacggccgg aagagaatgc tggcctctgc cggcgaactg cagaagggaa acgaactggc
       4141 cctgccctcc aaatatgtga acttcctgta cctggccagc cactatgaga agctgaaggg
       4201 ctcccccgag gataatgagc agaaacagct gtttgtggaa cagcacaagc actacctgga
       4261 cgagatcatc gagcagatca gcgagttctc caagagagtg atcctggccg acgctaatct
       4321 ggacaaagtg ctgtccgcct acaacaagca ccgggataag cccatcagag agcaggccga
       4381 gaatatcatc cacctgttta ccctgaccaa tctgggagcc cctgccgcct tcaagtactt
       4441 tgacaccacc atcgaccgga agaggtacac cagcaccaaa gaggtgctgg acgccaccct
       4501 gatccaccag agcatcaccg gcctgtacga gacacggatc gacctgtctc agctgggagg
       4561 cgacagcccc aagaagaaga gaaaggtgga ggccagctaa gaattcAATA AAAGATCTTT
       4621 ATTTTCATTA GATCTGTGTG TTGGTTTTTT GTGTgcggcc gcaggaaccc ctagtgatgg
       4681 agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg
       4741 cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc
       4801 aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac
       4861 gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
       4921 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
       4981 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
       5041 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga
       5101 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
       5161 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggg
       5221 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
       5281 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg
       5341 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
       5401 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
       5461 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
       5521 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
       5581 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
       5641 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
       5701 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
       5761 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
       5821 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
       5881 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
       5941 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
       6001 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
       6061 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
       6121 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
       6181 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
       6241 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
       6301 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
       6361 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
       6421 agccggtgag cgtggaagcc gcggtatcat tgcagcactg gggccagatg gtaagccctc
       6481 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
       6541 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
       6601 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
       6661 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
       6721 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
       6781 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
       6841 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
       6901 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
       6961 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
       7021 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       7081 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
       7141 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
       7201 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
       7261 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
       7321 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
       7381 ctggcctttt gctcacatgt
//

