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logicgate1.png Synthetic Biology: Bacterial


Browse Addgene's collection of synthetic biology plasmids for use in bacteria.






Bacterial Plasmids

Search the table by keyword or sort by the table headings. Click on the publication link to view all plasmids available from the article. Click on the PI link to view all plasmids available from this depositor's lab.

Plasmid Purpose Gene/Insert Vector Type PI Publication
pFIPinversion switchBacterial Expression, Synthetic Biology Keasling A tightly regulated inducible expression system utilizing the fim inversion recombination switch. Biotechnol Bioeng. 2006 May 5. 94(1):1-4.
pPro24Bacterial Expression, Synthetic Biology Keasling A propionate-inducible expression system for enteric bacteria. Appl Environ Microbiol. 2005 Nov . 71(11):6856-62.
pPro18Bacterial Expression, Synthetic Biology Keasling A propionate-inducible expression system for enteric bacteria. Appl Environ Microbiol. 2005 Nov . 71(11):6856-62.
pPro18-cmBacterial Expression, Synthetic Biology Keasling A propionate-inducible expression system for enteric bacteria. Appl Environ Microbiol. 2005 Nov . 71(11):6856-62.
pPro18-kmBacterial Expression, Synthetic Biology Keasling A propionate-inducible expression system for enteric bacteria. Appl Environ Microbiol. 2005 Nov . 71(11):6856-62.
pPro30Bacterial Expression, Synthetic Biology Keasling A propionate-inducible expression system for enteric bacteria. Appl Environ Microbiol. 2005 Nov . 71(11):6856-62.
pPro33Bacterial Expression, Synthetic Biology Keasling A propionate-inducible expression system for enteric bacteria. Appl Environ Microbiol. 2005 Nov . 71(11):6856-62.
pPro7(E)-gfpGFPuvBacterial Expression, Synthetic Biology Keasling A Salmonella-based, propionate-inducible, expression system for Salmonella enterica. Gene. 2006 Aug 1. 377():6-11.
pPro24(s)-gfpGFPuv (Other)Bacterial Expression, Synthetic Biology Keasling Propionate-regulated high-yield protein production in Escherichia coli. Biotechnol Bioeng. 2006 Apr 5. 93(5):912-8.
pBAD33MevTMevT (Saccharomyces cerevisiae)Bacterial Expression, Synthetic Biology Keasling Engineering a mevalonate pathway in Escherichia coli for production of terpenoids. Nat Biotechnol. 2003 Jul . 21(7):796-802.
pMevT (Saccharomyces cerevisiae)Bacterial Expression, Synthetic Biology Keasling Engineering a mevalonate pathway in Escherichia coli for production of terpenoids. Nat Biotechnol. 2003 Jul . 21(7):796-802.
pMBImevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase (Saccharomyces cerevisiae)Bacterial Expression, Synthetic Biology Keasling Engineering a mevalonate pathway in Escherichia coli for production of terpenoids. Nat Biotechnol. 2003 Jul . 21(7):796-802.
pMBISmevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase, FPP synthase (Saccharomyces cerevisiae)Bacterial Expression, Synthetic Biology Keasling Engineering a mevalonate pathway in Escherichia coli for production of terpenoids. Nat Biotechnol. 2003 Jul . 21(7):796-802.
pMevBmevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse (Saccharomyces cerevisiae)Bacterial Expression, Synthetic Biology Keasling Engineering a mevalonate pathway in Escherichia coli for production of terpenoids. Nat Biotechnol. 2003 Jul . 21(7):796-802.
pBAD24MevTMevT (Saccharomyces cerevisiae)Bacterial Expression, Synthetic Biology Keasling Balancing a heterologous mevalonate pathway for improved isoprenoid production in Escherichia coli. Metab Eng. 2007 Mar . 9(2):193-207.
pBdxsdxsBacterial Expression, Synthetic Biology Keasling Metabolic engineering of the nonmevalonate isopentenyl diphosphate synthesis pathway in Escherichia coli enhances lycopene production. Biotechnol Bioeng. 2001 Feb 20. 72(4):408-15.
pBAD*RFPEC2E. coli optimized red flourescent protein (RFPec2)Bacterial Expression, Synthetic Biology Keasling Optimization of DsRed production in Escherichia coli: effect of ribosome binding site sequestration on translation efficiency. Biotechnol Bioeng. 2005 Dec 5. 92(5):553-8.
p70rgE. coli optimized red flourescent protein (RFPec), GFPuvBacterial Expression, Synthetic Biology Keasling Combinatorial engineering of intergenic regions in operons tunes expression of multiple genes. Nat Biotechnol. 2006 Aug . 24(8):1027-32.
p70rg 1E. coli optimized red flourescent protein (RFPec), GFPuvBacterial Expression, Synthetic Biology Keasling Combinatorial engineering of intergenic regions in operons tunes expression of multiple genes. Nat Biotechnol. 2006 Aug . 24(8):1027-32.
p70rg 5E. coli optimized red flourescent protein (RFPec), GFPuvBacterial Expression, Synthetic Biology Keasling Combinatorial engineering of intergenic regions in operons tunes expression of multiple genes. Nat Biotechnol. 2006 Aug . 24(8):1027-32.
p70rg 11E. coli optimized red flourescent protein (RFPec), GFPuvBacterial Expression, Synthetic Biology Keasling Combinatorial engineering of intergenic regions in operons tunes expression of multiple genes. Nat Biotechnol. 2006 Aug . 24(8):1027-32.
p70rg 15E. coli optimized red flourescent protein (RFPec), GFPuvBacterial Expression, Synthetic Biology Keasling Combinatorial engineering of intergenic regions in operons tunes expression of multiple genes. Nat Biotechnol. 2006 Aug . 24(8):1027-32.
pET30-R5-GFPpET30-GFPBacterial Expression, Synthetic Biology Keasling Isotopomer distributions in amino acids from a highly expressed protein as a proxy for those from total protein. Anal Chem. 2008 Feb 1. 80(3):886-90.
pSPK1polyphosphate kinaseBacterial Expression, Synthetic Biology Keasling Manipulation of independent synthesis and degradation of polyphosphate in Escherichia coli for investigation of phosphate secretion from the cell. Appl Environ Microbiol. 1997 May . 63(5):1689-95.
pSVD5exopolyphosphataseBacterial Expression, Synthetic Biology Keasling Manipulation of independent synthesis and degradation of polyphosphate in Escherichia coli for investigation of phosphate secretion from the cell. Appl Environ Microbiol. 1997 May . 63(5):1689-95.
pPro24-gfpGFPuvBacterial Expression, Synthetic Biology Keasling A propionate-inducible expression system for enteric bacteria. Appl Environ Microbiol. 2005 Nov . 71(11):6856-62.
pJAT13promoter CP13Bacterial Expression, Synthetic Biology Keasling Homogeneous expression of the P(BAD) promoter in Escherichia coli by constitutive expression of the low-affinity high-capacity AraE transporter. Microbiology. 2001 Dec . 147(Pt 12):3241-7.
pJAT13araEaraEBacterial Expression, Synthetic Biology Keasling Homogeneous expression of the P(BAD) promoter in Escherichia coli by constitutive expression of the low-affinity high-capacity AraE transporter. Microbiology. 2001 Dec . 147(Pt 12):3241-7.
pAK02arabinose transporter araE under control of PtacBacterial Expression, Synthetic Biology Keasling Regulatable arabinose-inducible gene expression system with consistent control in all cells of a culture. J Bacteriol. 2000 Dec . 182(24):7029-34.
pAC-PHYTcrtE, crtBBacterial Expression, Synthetic Biology Keasling Controlling the metabolic flux through the carotenoid pathway using directed mRNA processing and stabilization. Metab Eng. 2001 Oct . 3(4):313-21.
pTrcHUMgamma-humulene synthaseBacterial Expression, Synthetic Biology Keasling Designed divergent evolution of enzyme function. Nature. 2006 Apr 20. 440(7087):1078-82.
pADScodon-optimized synthetic amorphadiene synthaseBacterial Expression, Synthetic Biology Keasling Engineering a mevalonate pathway in Escherichia coli for production of terpenoids. Nat Biotechnol. 2003 Jul . 21(7):796-802.
pBAD18HMGRtruncated 3-hydroxy-3-methyl-glutaryl-coenzyme A Reductase (Saccharomyces cerevisiae)Bacterial Expression, Synthetic Biology Keasling Balancing a heterologous mevalonate pathway for improved isoprenoid production in Escherichia coli. Metab Eng. 2007 Mar . 9(2):193-207.
pSB77thiosulfate reductaseBacterial Expression, Synthetic Biology Keasling Engineering hydrogen sulfide production and cadmium removal by expression of the thiosulfate reductase gene (phsABC) from Salmonella enterica serovar typhimurium in Escherichia coli. Appl Environ Microbiol. 2000 Sep . 66(9):3939-44.
pSB74thiosulfate reductaseBacterial Expression, Synthetic Biology Keasling Engineering hydrogen sulfide production and cadmium removal by expression of the thiosulfate reductase gene (phsABC) from Salmonella enterica serovar typhimurium in Escherichia coli. Appl Environ Microbiol. 2000 Sep . 66(9):3939-44.
pDUET-ctCPR-nAMOp450 reductase and native Amorphadiene oxidaseBacterial Expression, Synthetic Biology Keasling Engineering Escherichia coli for production of functionalized terpenoids using plant P450s. Nat Chem Biol. 2007 May . 3(5):274-7.
pDUET-ctCPR-sAMOp450 reductase and codon optimized amorphadiene oxidaseBacterial Expression, Synthetic Biology Keasling Engineering Escherichia coli for production of functionalized terpenoids using plant P450s. Nat Chem Biol. 2007 May . 3(5):274-7.
pDUET-ctCPR-trAMOp450 reductase and truncated amorphadiene oxidaseBacterial Expression, Synthetic Biology Keasling Engineering Escherichia coli for production of functionalized terpenoids using plant P450s. Nat Chem Biol. 2007 May . 3(5):274-7.
pDUET-ctCPR-BovAMOp450 reductase, and amorphadiene oxidaseBacterial Expression, Synthetic Biology Keasling Engineering Escherichia coli for production of functionalized terpenoids using plant P450s. Nat Chem Biol. 2007 May . 3(5):274-7.
pDUET-ctCPR-A13AMOp450 reductase and amorphadiene oxidaseBacterial Expression, Synthetic Biology Keasling Engineering Escherichia coli for production of functionalized terpenoids using plant P450s. Nat Chem Biol. 2007 May . 3(5):274-7.
pDUET-ctCPR-A17AMOp450 reductase and amorphadiene oxidaseBacterial Expression, Synthetic Biology Keasling Engineering Escherichia coli for production of functionalized terpenoids using plant P450s. Nat Chem Biol. 2007 May . 3(5):274-7.
pDUET-aaCPRct-A13AMOp450 reductase and Amorphadiene oxidaseBacterial Expression, Synthetic Biology Keasling Engineering Escherichia coli for production of functionalized terpenoids using plant P450s. Nat Chem Biol. 2007 May . 3(5):274-7.
pCWori-A13AMO-aaCPRctAmorphadiene oxidase and p450 reductaseBacterial Expression, Synthetic Biology Keasling Engineering Escherichia coli for production of functionalized terpenoids using plant P450s. Nat Chem Biol. 2007 May . 3(5):274-7.
pESC-URA::CYP71AV1/CPRamorphadiene oxidase and p450 reductaseYeast Expression, Synthetic Biology Keasling Production of the antimalarial drug precursor artemisinic acid in engineered yeast. Nature. 2006 Apr 13. 440(7086):940-3.
pRS425ADSamorphadiene synthaseYeast Expression, Synthetic Biology Keasling Production of the antimalarial drug precursor artemisinic acid in engineered yeast. Nature. 2006 Apr 13. 440(7086):940-3.
pESC-leu2d(empty)Yeast Expression, Synthetic Biology Keasling Production of the antimalarial drug precursor artemisinic acid in engineered yeast. Nature. 2006 Apr 13. 440(7086):940-3.
pCph8pCph8Bacterial Expression, Synthetic Biology Voigt Synthetic biology: engineering Escherichia coli to see light. Nature. 2005 Nov 24. 438(7067):441-2.
pHL32 (in cHNL135)Bacterial Expression, Synthetic Biology Lim A multistep epigenetic switch enables the stable inheritance of DNA methylation states. Nat Genet. 2007 Feb . 39(2):269-75.
pKS2'tesABacterial Expression, Synthetic Biology Keasling Microbial production of fatty-acid-derived fuels and chemicals from plant biomass. Nature. 2010 Jan 28;463(7280):559-62.
pKS13pdc, adhBBacterial Expression, Synthetic Biology Keasling Microbial production of fatty-acid-derived fuels and chemicals from plant biomass. Nature. 2010 Jan 28;463(7280):559-62.
pKS17pdc, adhB, atfABacterial Expression, Synthetic Biology Keasling Microbial production of fatty-acid-derived fuels and chemicals from plant biomass. Nature. 2010 Jan 28;463(7280):559-62.
pKS18mFar1 (Mus musculus)Bacterial Expression, Synthetic Biology Keasling Microbial production of fatty-acid-derived fuels and chemicals from plant biomass. Nature. 2010 Jan 28;463(7280):559-62.
pKS100fadDBacterial Expression, Synthetic Biology Keasling Microbial production of fatty-acid-derived fuels and chemicals from plant biomass. Nature. 2010 Jan 28;463(7280):559-62.
pKS101FAA2 (Saccharomyces cerevisiae)Bacterial Expression, Synthetic Biology Keasling Microbial production of fatty-acid-derived fuels and chemicals from plant biomass. Nature. 2010 Jan 28;463(7280):559-62.
pKS104fadD (M3351), atfABacterial Expression, Synthetic Biology Keasling Microbial production of fatty-acid-derived fuels and chemicals from plant biomass. Nature. 2010 Jan 28;463(7280):559-62.
pKS1'tesABacterial Expression, Synthetic Biology Keasling Microbial production of fatty-acid-derived fuels and chemicals from plant biomass. Nature. 2010 Jan 28;463(7280):559-62.
pGB3xynBBacterial Expression, Synthetic Biology Keasling Microbial production of fatty-acid-derived fuels and chemicals from plant biomass. Nature. 2010 Jan 28;463(7280):559-62.
pJT103PompC-lacZBacterial Expression, Synthetic Biology Voigt A synthetic genetic edge detection program. Cell. 2009 Jun 26. 137(7):1272-81.
pJT104Edge detector delta luxIBacterial Expression, Synthetic Biology Voigt A synthetic genetic edge detection program. Cell. 2009 Jun 26. 137(7):1272-81.
pJT105Edge detector delta cIBacterial Expression, Synthetic Biology Voigt A synthetic genetic edge detection program. Cell. 2009 Jun 26. 137(7):1272-81.
pEDL3Edge detectorBacterial Expression, Synthetic Biology Voigt A synthetic genetic edge detection program. Cell. 2009 Jun 26. 137(7):1272-81.
pPro29b(+)Bacterial Expression, Synthetic Biology Keasling Keasling Lab (unpublished)
pMLLKA-J72016N terminal 6X His tag with RBSSynthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLKA-J72017N terminal avi tag with RBSSynthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLKA-J72018N terminal E tag with RBSSynthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLKA-J72019N terminal Flag tag with RBSSynthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLKA-J72020N terminal HA tag with RBSSynthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLKA-J72021N terminal HSV tag with RBSSynthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLKA-J72022N terminal myc tag with RBSSynthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLKA-J72023N terminal S tag with RBSSynthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLKA-J72024N terminal Strep tag with RBSSynthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLKA-J72025N terminal T7 tag with RBSSynthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLKA-J72026N terminal V5 tag with RBSSynthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLKA-J72027N terminal VSV tag with RBSSynthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLCK-J72028C terminal 6XHIS tagSynthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLCK-J72029C terminal avi tagSynthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLCK-J72030C terminal E tagSynthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLCK-K112603C terminal Flag tagSynthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLCK-K112507C terminal HA tagSynthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLCK-J72031C terminal HSV tagSynthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLCK-K112503C terminal myc tagSynthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLCK-J72032C terminal S tagSynthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLCK-J72033C terminal Strep tagSynthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLCK-J72034C terminal T7 tagSynthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLCK-J72035C terminal V5 tagSynthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLCK-J72036C terminal VSV tagSynthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLCK-J72037Med7 aa102-205 for N-terminal taggingSynthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLCK-J72038Med21 aa1-132 for N-terminal tagging (Saccharomyces cerevisiae)Synthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLKA-J72039Med7 aa102-205 for C-terminal tagging (Saccharomyces cerevisiae)Synthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLKA-J72040Med21 aa1-132 for C-terminal tagging (Saccharomyces cerevisiae)Synthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pMLLAC-J72041Tev Protease Cleavage siteSynthetic Biology ; 2ab assembly vector Anderson J.C. Anderson Lab Plasmids (unpublished)
pTRKH3-slpGFPsurface (S)-layer protein promoter region fused with modified green fluorescent protein 5Bacterial Expression Lizier Comparison of expression vectors in Lactobacillus reuteri strains. FEMS Microbiol Lett. 2010 Jul 1. 308(1):8-15.
pTRKH3-ermGFPerythromycin ribosomal methylase promoter region fused with modified green fluorescent protein 5Bacterial Expression Lizier Comparison of expression vectors in Lactobacillus reuteri strains. FEMS Microbiol Lett. 2010 Jul 1. 308(1):8-15.
pBS-ldhGFPlactate dehydrogenase promoter region fused with modified green fluorescent protein 5Bacterial Expression Lizier Comparison of expression vectors in Lactobacillus reuteri strains. FEMS Microbiol Lett. 2010 Jul 1. 308(1):8-15.
pBS-slpGFPsurface (S)-layer protein promoter region fused with modified green fluorescent protein 5Bacterial Expression Lizier Comparison of expression vectors in Lactobacillus reuteri strains. FEMS Microbiol Lett. 2010 Jul 1. 308(1):8-15.
pBS-ermGFPerythromycin ribosomal methylase promoter region fused with modified green fluorescent protein 5Bacterial Expression Lizier Comparison of expression vectors in Lactobacillus reuteri strains. FEMS Microbiol Lett. 2010 Jul 1. 308(1):8-15.
HL 818 (=HL 716 + pHL 98)pLlacO-1:DsrA-mcherry, pLtetO-1:rpoS::gfp (Other)Bacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 839 (=HL 716 + pHL 108)pLlacO-1:MicC-mCherry, pLtetO-1:ompC::gfp (Other)Bacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 1178 (=HL 862 + pHL 282)pLtetO-1:mCherry, pLlacO-1:ompC::gfpBacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 1329 (=HL 865 + pHL 344)pLtetO-1:mCherry, pLlacO-1:rpoS::gfpBacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 1474 (=HL 862 + pHL 404)pLtetO-1:MicC, pLlacO-1:hfq, pLtetO-1:ompC::gfpBacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 2300 (=HL 716 + pHL 745)pLtetO-1:RhyB-mCherry, pLlacO-1:sodB::gfpBacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 2304 (=HL 716 + pHL 269)pLtetO-1:MicC-mCherry, pLlacO-1:ompC::gfpBacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 2305 (=HL 716 + pHL 765)pLtetO-1:MicC, pLtetO-1:DsrA, pLlacO-1:ompC::gfpBacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 2307 (=HL 716 + pHL 777)pLtetO-1:MicC, pLtetO-1:RhyB, pLlacO-1:ompC::gfpBacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 2308 (=HL 716 + pHL 814)pLtetO-1:MicC, pLtetO-1:OxyS, pLlacO-1:ompC::gfpBacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 2312 (=HL 716 + pHL 787)pLtetO-1:MicC, pLtetO-1:rpoS::mCherry, pLlacO-1:ompC::gfpBacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 2314 (=HL 716 + pHL 818)pLtetO-1:MicC, pLtetO-1:fhlA::mCherry, pLlacO-1:ompC::gfpBacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 2625 (=HL 716 + pHL 908)pLlacO-1:RhyB::mCherry, pLtetO-1m9:sodB::gfpBacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 2627 (=HL 770 + pHL 908)pLlacO-1:RhyB::mCherry, pLtetO-1m9:sodB::gfpBacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 2817 (=HL 716 + pHL 720)pLtetO-1:mCherry, pLlacO-1:sodB::gfpBacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 2818 (=HL 865 + pHL 489)pLtetO-1:DsrA, pLlacO-1:hfq, pLtetO-1:rpoS::gfpBacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 2839 (=HL 2752 + pHL 720)pLtetO-1:mCherry, pLlacO-1:sodB::gfpBacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 2869 (=HL 2752 + pHL 991)pLtetO-1:RhyB, pLlacO-1:hfq, pLtetO-1m9:sodB::gfpBacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 3076 (=HL 716 + pHL 1069)pCon:MicC, pLlacO-1:sodB::gfpBacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 3151 (=HL 716 + pHL 1037)pLtetO-1:DsrA, pCon:MicC, pLlacO-1:rpoS::gfpBacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 3152 (=HL 716 + pHL 1056)pCon:MicC, pLlacO-1:rpoS::gfpBacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 3186 (=HL 716 + pHL 282)pLtetO-1:mCherry, pLlacO-1:ompC::gfpBacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 3326 (=HL 716 + pHL 1008)pLlacO-1:DsrA-mCherry, pLtetO-1:RBS (st7) gfpBacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 3327 (=HL 716 + pHL 1009)pLlacO-1:MicC-mCherry, pLtetO-1:RBS (st7) gfpBacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 3330 (=HL 770 + pHL 1009)pLlacO-1:MicC-mCherry, pLtetO-1:RBS (st7) gfpBacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 3347 (=HL 3338 + pHL 720)pLtetO-1:mCherry, pLlacO-1:sodB::gfpBacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 3357 (=HL 716 + pHL 1191)pLlacO-1:OxyS-mCherry, pLtetO-1:RBS (st7) gfpBacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 3375 (=HL 865 + pHL 1008)pLlacO-1:DsrA-mCherry, pLtetO-1:RBS (st7) gfpBacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 3395 (=HL 862 + pHL 1009)pLlacO-1:MicC-mCherry, pLtetO-1:RBS (st7) gfpBacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 3448 (=HL 3425 + pHL 1191)pLlacO-1:OxyS-mCherry, pLtetO-1:RBS (st7) gfpBacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 3449 (=HL 3262 + pHL 1179)pLtetO-1:mCherry, pLlacO-1:fhlA::gfpBacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 3450 (=HL 3262 + pHL 1191)pLlacO-1:OxyS-mCherry, pLtetO-1:RBS (st7) gfpBacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 3545 (=HL 770 + pHL 1213)pLlacO-1:hfq, pLtetO-1:RBS (st7) gfpBacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 3612 (=HL 716 + pHL 841)pLtetO-1:RhyB-mCherry, pLlacO-1:ompC::gfpBacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 3619 (=HL 716 + pHL 1228)pLtetO-1:DsrA-mCherry, pLlacO-1:ompC::gfpBacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 3722 (=HL 716 + pHL 404)pLtetO-1:MicC, pLlacO-1:hfq, pLtetO-1:ompC::gfpBacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 3723 (=HL 716 + pHL 489)pLtetO-1:DsrA, pLlacO-1:hfq, pLtetO-1:rpoS::gfpBacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 3724 (=HL 716 + pHL 991)pLtetO-1:RhyB, pLlacO-1:hfq, pLtetO-1m9:sodB::gfpBacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 3858 (=HL 716 + pHL 1304)pLtetO-1:rpoS::mCherry, pLlacO-1:ompC::gfpBacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 3859 (=HL 716 + pHL 1305)pLtetO-1:fhlA::mCherry, pLlacO-1:ompC::gfpBacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 3904 (=HL 770 + pHL 991)pLtetO-1:RhyB, pLlacO-1:hfq, pLtetO-1m9:sodB::gfpBacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 4120 (=HL 716 + pHL 1370)pLtetO-1:DsrA, pCon:T7(st3) (900bps), pLlacO-1:rpoS::gfpBacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 4175 (=HL 716 + pHL 1391)pLlacO-1:rpoS::gfpBacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 4178 (=HL 862 + pHL 1360)pLlacO-1:hfq, pLtetO-1:ompC::gfpBacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 4179 (=HL 865 + pHL 1381)pLlacO-1:hfq, pLtetO-1:rpoS::gfpBacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 4180 (=HL 2752 + pHL 1358)pLlacO-1:hfq, pLtetO-1m9:sodB::gfpBacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 4202 (=HL 716 + pHL 1389)pLtetO-1:DsrA, pLlacO-1:rpoS::gfpBacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 4212 (=HL 716 + pHL 1414)pCon:fhlA::T7noRBS(900bps), pLlacO-1:rpoS::gfpBacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
HL 4218 (=HL 716 + pHL 1411)pLtetO-1:DsrA, pCon:fhlA:T7noRBS(900bps), pLlacO-1:rpoS::gfp (Other)Bacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
SS13 lac-inducible GFP reporter circuitGFPBacterial Expression, Synthetic Biology Sauro Designing and engineering evolutionary robust genetic circuits J Biol Eng. 2010 Nov 1;4:12
pJT106bred light inverterBacterial Expression, Synthetic Biology Voigt Multichromatic control of gene expression in Escherichia coli. J Mol Biol. 2011 Jan 14. 405(2):315-24.
pJT106b3red light inverter with lacZ outputBacterial Expression, Synthetic Biology Voigt Multichromatic control of gene expression in Escherichia coli. J Mol Biol. 2011 Jan 14. 405(2):315-24.
pJT116cca cluster from Synechocystis PCC6803Bacterial Expression, Synthetic Biology Voigt Multichromatic control of gene expression in Escherichia coli. J Mol Biol. 2011 Jan 14. 405(2):315-24.
pJT118pJT118Bacterial Expression, Synthetic Biology Voigt Multichromatic control of gene expression in Escherichia coli. J Mol Biol. 2011 Jan 14. 405(2):315-24.
pJT122pJT122Bacterial Expression, Synthetic Biology Voigt Multichromatic control of gene expression in Escherichia coli. J Mol Biol. 2011 Jan 14. 405(2):315-24.
pUCBB-eGFPeGFPBacterial Expression, Synthetic Biology Schmidt-dannert Optimized compatible set of BioBrick vectors for metabolic pathway engineering. Appl Microbiol Biotechnol. 2011 Dec;92(6):1275-86. Epub 2011 Oct 28.
pBBRBB-eGFPeGFPBacterial Expression, Synthetic Biology Schmidt-dannert Optimized compatible set of BioBrick vectors for metabolic pathway engineering. Appl Microbiol Biotechnol. 2011 Dec;92(6):1275-86. Epub 2011 Oct 28.
pCDFBB-eGFPegfpSynthetic Biology Schmidt-dannert Optimized compatible set of BioBrick vectors for metabolic pathway engineering. Appl Microbiol Biotechnol. 2011 Dec;92(6):1275-86. Epub 2011 Oct 28.
pACBB-eGFPeGFPSynthetic Biology Schmidt-dannert Optimized compatible set of BioBrick vectors for metabolic pathway engineering. Appl Microbiol Biotechnol. 2011 Dec;92(6):1275-86. Epub 2011 Oct 28.
pUCBB-pT7-eGFPeGFPBacterial Expression, Synthetic Biology Schmidt-dannert Optimized compatible set of BioBrick vectors for metabolic pathway engineering. Appl Microbiol Biotechnol. 2011 Dec;92(6):1275-86. Epub 2011 Oct 28.
pUCBB-pBAD-eGFPeGFPSynthetic Biology Schmidt-dannert Optimized compatible set of BioBrick vectors for metabolic pathway engineering. Appl Microbiol Biotechnol. 2011 Dec;92(6):1275-86. Epub 2011 Oct 28.
pUCBB-ptet-eGFPeGFPSynthetic Biology Schmidt-dannert Optimized compatible set of BioBrick vectors for metabolic pathway engineering. Appl Microbiol Biotechnol. 2011 Dec;92(6):1275-86. Epub 2011 Oct 28.
pACBB-TetT-LVATetRBacterial Expression, Synthetic Biology Schmidt-dannert Optimized compatible set of BioBrick vectors for metabolic pathway engineering. Appl Microbiol Biotechnol. 2011 Dec;92(6):1275-86. Epub 2011 Oct 28.
pUCBB-oriToriTBacterial Expression, Synthetic Biology Schmidt-dannert Optimized compatible set of BioBrick vectors for metabolic pathway engineering. Appl Microbiol Biotechnol. 2011 Dec;92(6):1275-86. Epub 2011 Oct 28.
pUCBB-ntH6-eGFPeGFPBacterial Expression, Synthetic Biology Schmidt-dannert Optimized compatible set of BioBrick vectors for metabolic pathway engineering. Appl Microbiol Biotechnol. 2011 Dec;92(6):1275-86. Epub 2011 Oct 28.
pUCBB-ctH6-eGFPeGFPSynthetic Biology Schmidt-dannert Optimized compatible set of BioBrick vectors for metabolic pathway engineering. Appl Microbiol Biotechnol. 2011 Dec;92(6):1275-86. Epub 2011 Oct 28.
pUCBB-lacZaLacZ-alphaBacterial Expression, Synthetic Biology Schmidt-dannert Optimized compatible set of BioBrick vectors for metabolic pathway engineering. Appl Microbiol Biotechnol. 2011 Dec;92(6):1275-86. Epub 2011 Oct 28.
pBbA5c-MevT-MBISAtoB(o)-HMGS(o)-HMGR(o)-MK(o)-PMK(o)-PMD-Idi-IspA (Saccharomyces cerevisiae)Bacterial Expression, Synthetic Biology Lee Identification and microbial production of a terpene-based advanced biofuel. Nat Commun. 2011 Sep 27;2:483. doi: 10.1038/ncomms1494.
pBbA5c-MevT(CO)-MBIS(CO, ispA)AtoB(co)-HMGS(co)-HMGR(co)-MK(co)-PMK(co)-PMD-Idi-IspA (Saccharomyces cerevisiae)Bacterial Expression, Synthetic Biology Lee Identification and microbial production of a terpene-based advanced biofuel. Nat Commun. 2011 Sep 27;2:483. doi: 10.1038/ncomms1494.
pBbA5c-MevT(CO)-T1-MBIS(CO, ispA)AtoB(co)-HMGS(co)-HMGR(co)-pTrc-MK(co)-PMK(co)-PMD-Idi-IspA (Saccharomyces cerevisiae)Bacterial Expression, Synthetic Biology Lee Identification and microbial production of a terpene-based advanced biofuel. Nat Commun. 2011 Sep 27;2:483. doi: 10.1038/ncomms1494.
pTrcAgBIS (CO)Bisabolene SynthaseBacterial Expression, Synthetic Biology Lee Identification and microbial production of a terpene-based advanced biofuel. Nat Commun. 2011 Sep 27;2:483. doi: 10.1038/ncomms1494.
pBbE8a-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbE8c-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbE8k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbA8a-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbA8c-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbA8k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbS8a-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbS8c-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbS8k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbE5a-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbE5c-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbE5k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbA5a-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbA5c-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbA5k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbS5a-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbS5c-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbS5k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbE6a-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbE6c-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbE6k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbA6a-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbA6c-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbA6k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbS6a-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbS6c-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbS6k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbE4a-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbE4c-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbE4k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbA4a-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbA4c-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbA4k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbS4a-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbS4c-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbS4k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbE3a-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbE3c-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbE3k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbA3a-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbA3c-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbA3k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbS3a-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbS3c-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbS3k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbE7a-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbE7c-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbE7k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbA7a-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbA7c-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbA7k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbS7c-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbS7k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbE2a-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbE2c-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbE2k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbA2a-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbA2c-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbA2k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbS2a-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbS2c-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbS2k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbE1a-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbE1c-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbE1k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbA1a-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbA1c-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbA1k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbS1a-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbS1c-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbS1k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbB1a-GFPGFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbB1c-GFPGFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbB1k-GFPGFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbB2a-GFPGFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbB2c-GFPGFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbB2k-GFPGFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbB3a-GFPGFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbB3c-GFPGFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbB3k-GFPGFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbB4a-GFPGFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbB4c-GFPGFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbB4k-GFPGFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbB5a-GFPGFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbB5c-GFPGFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbB5k-GFPGFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbB6a-GFPGFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbB6c-GFPGFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbB6k-GFPGFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbB7a-GFPGFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbB7c-GFPGFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbB7k-GFPGFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbB8a-GFPGFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbB8c-GFPGFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbB8k-GFPGFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbE0c-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbE0k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbB0c-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbS0a-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbS0k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbB0k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbB0a-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbE0a-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbA0c-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbA0k-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pBbA0a-RFPRFP (Synthetic)Bacterial Expression, Synthetic Biology Keasling BglBrick vectors and datasheets: A synthetic biology platform for gene expression. J Biol Eng. 2011 Sep 20;5:12.
pRSLeu2d-BISoptBisabolene SynthaseYeast Expression, Synthetic Biology Lee Identification and microbial production of a terpene-based advanced biofuel. Nat Commun. 2011 Sep 27;2:483. doi: 10.1038/ncomms1494.
pAC-4CL14-coumaroyl CoA ligase 1 (Arabidopsis thaliana)Bacterial Expression, Synthetic Biology Schmidt-dannert Biosynthesis of plant-specific stilbene polyketides in metabolically engineered Escherichia coli. BMC Biotechnol. 2006 Mar 21;6:22.
pUC-STSStilbene synthaseSynthetic Biology Schmidt-dannert Biosynthesis of plant-specific stilbene polyketides in metabolically engineered Escherichia coli. BMC Biotechnol. 2006 Mar 21;6:22.
pRibo BsaHindBacterial Expression, Synthetic Biology ; Mycobacteria replicating vector Bertozzi A riboswitch-based inducible gene expression system for mycobacteria. PLoS One. 2012;7(1):e29266. Epub 2012 Jan 18.
pRibo EcoHindBacterial Expression, Synthetic Biology ; Mycobacteria replicating vector Bertozzi A riboswitch-based inducible gene expression system for mycobacteria. PLoS One. 2012;7(1):e29266. Epub 2012 Jan 18.
pRiboS BsaHindBacterial Expression, Synthetic Biology ; Mycobacteria replicating vector Bertozzi A riboswitch-based inducible gene expression system for mycobacteria. PLoS One. 2012;7(1):e29266. Epub 2012 Jan 18.
pRiboS EcoHindBacterial Expression, Synthetic Biology Bertozzi A riboswitch-based inducible gene expression system for mycobacteria. PLoS One. 2012;7(1):e29266. Epub 2012 Jan 18.
pST5552Mycobacteria replicating vector expressing eGFP.eGFPBacterial Expression, Synthetic Biology ; Mycobacteria replicating vector Bertozzi A riboswitch-based inducible gene expression system for mycobacteria. PLoS One. 2012;7(1):e29266. Epub 2012 Jan 18.
pST5832lacZBacterial Expression, Synthetic Biology ; Mycobacteria replicating vector Bertozzi A riboswitch-based inducible gene expression system for mycobacteria. PLoS One. 2012;7(1):e29266. Epub 2012 Jan 18.
pCellulosepCelluloseSynthetic Biology Keasling Synthesis of three advanced biofuels from ionic liquid-pretreated switchgrass using engineered Escherichia coli. Proc Natl Acad Sci U S A. 2011 Dec 13;108(50):19949-54. Epub 2011 Nov 28.
pXylanpXylanSynthetic Biology Keasling Synthesis of three advanced biofuels from ionic liquid-pretreated switchgrass using engineered Escherichia coli. Proc Natl Acad Sci U S A. 2011 Dec 13;108(50):19949-54. Epub 2011 Nov 28.
pHL600CsrB, CsrABacterial Expression, Synthetic Biology Lim Rapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation. Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22.
pHL601CsrC, CsrABacterial Expression, Synthetic Biology Lim Rapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation. Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22.
pHL1355GFP, TetR, CsrABacterial Expression, Synthetic Biology Lim Rapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation. Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22.
pHL1530GFP, TetR, CsrABacterial Expression, Synthetic Biology Lim Rapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation. Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22.
pHL1559CsrBBacterial Expression, Synthetic Biology Lim Rapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation. Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22.
pHL1561CsrABacterial Expression, Synthetic Biology Lim Rapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation. Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22.
pHL1668GFP, TetR, CsrABacterial Expression, Synthetic Biology Lim Rapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation. Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22.
pHL1670GFP, TetR, CsrABacterial Expression, Synthetic Biology Lim Rapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation. Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22.
pHL661GFP, mCherryBacterial Expression, Synthetic Biology Lim Rapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation. Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22.
pHL662mCherry, GFPBacterial Expression, Synthetic Biology Lim Rapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation. Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22.
pHL1318TetR, CsrABacterial Expression, Synthetic Biology Lim Rapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation. Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22.
pHL1324GFP, TetR, CsrABacterial Expression, Synthetic Biology Lim Rapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation. Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22.
pHL 233ptet_ompC_gfp, pLac_st7 TetR_mCherrySynthetic Biology ; E. coli Lim Direct comparison of small RNA and transcription factor signaling. Nucleic Acids Res. 2012 May 22.
pHL 279ptet_ompC_gfp, pLac_st3 TetR_mCherrySynthetic Biology ; E. coli Lim Direct comparison of small RNA and transcription factor signaling. Nucleic Acids Res. 2012 May 22.
pHL 363pLar_rpoS_gfp, pLtet_dsrA_pLac_araCSynthetic Biology ; E. coli Lim Direct comparison of small RNA and transcription factor signaling. Nucleic Acids Res. 2012 May 22.
pHL 1569ptet_ompC_gfpAaV, pLac_micC_mCherrySynthetic Biology ; E. coli Lim Direct comparison of small RNA and transcription factor signaling. Nucleic Acids Res. 2012 May 22.
pHL 1571ptet_ompC_gfpAaV, pLac_st3 TetR_mCherrySynthetic Biology ; E. coli Lim Direct comparison of small RNA and transcription factor signaling. Nucleic Acids Res. 2012 May 22.
pSB4A5 JQ929581mKate2 (Synthetic), Superfolder GFP (Synthetic)Synthetic Biology ; BioBrick cloning vector Endy Rewritable digital data storage in live cells via engineered control of recombination directionality. Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):8884-9. Epub 2012 May 21.
pSB4A5 JQ929582mKate2 (Synthetic), Superfolder GFP (Synthetic)Synthetic Biology ; BioBrick cloning vector Endy Rewritable digital data storage in live cells via engineered control of recombination directionality. Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):8884-9. Epub 2012 May 21.
Phi80 JQ929581mKate2 (Synthetic), Superfolder GFP (Synthetic)Synthetic Biology ; Integration vector Endy Rewritable digital data storage in live cells via engineered control of recombination directionality. Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):8884-9. Epub 2012 May 21.
Phi80 JQ929582mKate2 (Synthetic), Superfolder GFP (Synthetic)Synthetic Biology ; Integration vector Endy Rewritable digital data storage in live cells via engineered control of recombination directionality. Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):8884-9. Epub 2012 May 21.
Strain Phi80 JQ929581mKate2 (Synthetic), Superfolder GFP (Synthetic)Synthetic Biology ; Integration vector Endy Rewritable digital data storage in live cells via engineered control of recombination directionality. Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):8884-9. Epub 2012 May 21.
Strain Phi80 JQ929582mKate2 (Synthetic), Superfolder GFP (Synthetic)Synthetic Biology ; Integration vector Endy Rewritable digital data storage in live cells via engineered control of recombination directionality. Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):8884-9. Epub 2012 May 21.
Strain HK022 JQ929581mKate2 (Synthetic), Superfolder GFP (Synthetic)Synthetic Biology ; Integration vector Endy Rewritable digital data storage in live cells via engineered control of recombination directionality. Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):8884-9. Epub 2012 May 21.
pBad-Xis/Int Reset FlipperBxb1-Excisionase, Bxb1 IntegraseSynthetic Biology ; BioBrick cloning vector Endy Rewritable digital data storage in live cells via engineered control of recombination directionality. Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):8884-9. Epub 2012 May 21.
RAD Module G8-C1SET Integrase, Bxb1 Excisonase, RESET IntegraseSynthetic Biology ; BioBrick cloning vector Endy Rewritable digital data storage in live cells via engineered control of recombination directionality. Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):8884-9. Epub 2012 May 21.
pBad-Int Set FlipperBxb1 IntegraseSynthetic Biology ; BioBrick cloning vector Endy Rewritable digital data storage in live cells via engineered control of recombination directionality. Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):8884-9. Epub 2012 May 21.
pBA2kIKmA2Kudzu Isoprene Synthase (Synthetic)Synthetic Biology Melis Engineering a platform for photosynthetic isoprene production in cyanobacteria, using Synechocystis as the model organism. Metab Eng. 2010 Jan;12(1):70-9. Epub 2009 Oct 13.
pBA2SkIKmA2Synechocystis kudzu IspSSynthetic Biology Melis Engineering a platform for photosynthetic isoprene production in cyanobacteria, using Synechocystis as the model organism. Metab Eng. 2010 Jan;12(1):70-9. Epub 2009 Oct 13.
pSTC0Bacterial Expression, Synthetic Biology Jaramillo De novo automated design of small RNA circuits for engineering synthetic riboregulation in living cells. Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep 4.
pSTC1Bacterial Expression, Synthetic Biology Jaramillo De novo automated design of small RNA circuits for engineering synthetic riboregulation in living cells. Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep 4.
pSTC0r1pLlacO-1+BBa_B0034+GFPBacterial Expression, Synthetic Biology Jaramillo De novo automated design of small RNA circuits for engineering synthetic riboregulation in living cells. Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep 4.
pRAJ11RAJ11 systemBacterial Expression, Synthetic Biology Jaramillo De novo automated design of small RNA circuits for engineering synthetic riboregulation in living cells. Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep 4.
pRAJ11mRAJ11 system (no transRNA)Bacterial Expression, Synthetic Biology Jaramillo De novo automated design of small RNA circuits for engineering synthetic riboregulation in living cells. Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep 4.
pRAJ12RAJ12 systemBacterial Expression, Synthetic Biology Jaramillo De novo automated design of small RNA circuits for engineering synthetic riboregulation in living cells. Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep 4.
pRAJ12mRAJ12 system (no transRNA)Bacterial Expression, Synthetic Biology Jaramillo De novo automated design of small RNA circuits for engineering synthetic riboregulation in living cells. Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep 4.
pRAJ12fRAJ12 system (no terminator for the transRNA)Bacterial Expression, Synthetic Biology Jaramillo De novo automated design of small RNA circuits for engineering synthetic riboregulation in living cells. Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep 4.
pRAJ21RAJ21 systemBacterial Expression, Synthetic Biology Jaramillo De novo automated design of small RNA circuits for engineering synthetic riboregulation in living cells. Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep 4.
pRAJ21mRAJ21 system (no transRNA)Bacterial Expression, Synthetic Biology Jaramillo De novo automated design of small RNA circuits for engineering synthetic riboregulation in living cells. Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep 4.
pRAJ22RAJ22 systemBacterial Expression, Synthetic Biology Jaramillo De novo automated design of small RNA circuits for engineering synthetic riboregulation in living cells. Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep 4.
pRAJ22mRAJ22 system (no transRNA)Bacterial Expression, Synthetic Biology Jaramillo De novo automated design of small RNA circuits for engineering synthetic riboregulation in living cells. Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep 4.
pRAJ23RAJ23 systemBacterial Expression, Synthetic Biology Jaramillo De novo automated design of small RNA circuits for engineering synthetic riboregulation in living cells. Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep 4.
pRAJ23mRAJ23 system (no transRNA)Bacterial Expression, Synthetic Biology Jaramillo De novo automated design of small RNA circuits for engineering synthetic riboregulation in living cells. Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep 4.
pRAJ31RAJ31 systemBacterial Expression, Synthetic Biology Jaramillo De novo automated design of small RNA circuits for engineering synthetic riboregulation in living cells. Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep 4.
pRAJ31mRAJ31 system (no transRNA)Bacterial Expression, Synthetic Biology Jaramillo De novo automated design of small RNA circuits for engineering synthetic riboregulation in living cells. Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep 4.
pRAJ31fRAJ31 system (no terminator for the transRNA)Bacterial Expression, Synthetic Biology Jaramillo De novo automated design of small RNA circuits for engineering synthetic riboregulation in living cells. Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep 4.
pRAJ11-12transRAJ11+cisRAJ12Bacterial Expression, Synthetic Biology Jaramillo De novo automated design of small RNA circuits for engineering synthetic riboregulation in living cells. Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep 4.
SW5Bistable switch using dynamic degradationBacterial Expression, Synthetic Biology Maharbiz A genetic bistable switch utilizing nonlinear protein degradation. J Biol Eng. 2012 Jul 9;6(1):9.
PLlacO-1-CImfLac inducible lambda repressorBacterial Expression, Synthetic Biology Maharbiz A genetic bistable switch utilizing nonlinear protein degradation. J Biol Eng. 2012 Jul 9;6(1):9.
PRM-GFPCI inducible sfGFPBacterial Expression, Synthetic Biology Maharbiz A genetic bistable switch utilizing nonlinear protein degradation. J Biol Eng. 2012 Jul 9;6(1):9.
pET21b-LOV-ipaALOV-ipaA (Synthetic)Bacterial Expression Kuhlman Designing photoswitchable peptides using the AsLOV2 domain. Chem Biol. 2012 Apr 20;19(4):507-17.
pAM1518psbAI promoter, luxCDECyanobacteria cloning vector Golden Application of bioluminescence to the study of circadian rhythms in cyanobacteria. Methods Enzymol. 2000;305:527-42.
pET21b-LOV-ipaA L514K L531ELOV-ipaA L514K L531E (Synthetic)Bacterial Expression Kuhlman Designing photoswitchable peptides using the AsLOV2 domain. Chem Biol. 2012 Apr 20;19(4):507-17.
BBa_J72110-BBa_J72094AraC-pBad + coiBacterial Expression, Synthetic Biology Anderson Scalable plasmid transfer using engineered P1-based phagemids. ACS Synth Biol. 2012 Dec 21;1(12):583-9. doi: 10.1021/sb300054p. Epub 2012 Aug 30.
BBa_J72110-BBa_J72097RhaRS-pRhaB + CoiBacterial Expression, Synthetic Biology Anderson Scalable plasmid transfer using engineered P1-based phagemids. ACS Synth Biol. 2012 Dec 21;1(12):583-9. doi: 10.1021/sb300054p. Epub 2012 Aug 30.
BBa_J72110-BBa_J72103AraC-pBad + coi + cin + repL + pacABacterial Expression, Synthetic Biology ; Phagemid Anderson Scalable plasmid transfer using engineered P1-based phagemids. ACS Synth Biol. 2012 Dec 21;1(12):583-9. doi: 10.1021/sb300054p. Epub 2012 Aug 30.
BBa_J72114-BBa_J72100lacZBacterial Expression, Synthetic Biology ; Phagemid Anderson Scalable plasmid transfer using engineered P1-based phagemids. ACS Synth Biol. 2012 Dec 21;1(12):583-9. doi: 10.1021/sb300054p. Epub 2012 Aug 30.
BBa_J72214-BBa_J72090VioABCDEBacterial Expression, Synthetic Biology ; phagemid Anderson Scalable plasmid transfer using engineered P1-based phagemids. ACS Synth Biol. 2012 Dec 21;1(12):583-9. doi: 10.1021/sb300054p. Epub 2012 Aug 30.
BBa_J72114-BBa_J72104RhaRS + pRhaB + LuxCDABE, VioABCE, LacZBacterial Expression, Luciferase, Synthetic Biology ; Phagemid Anderson Scalable plasmid transfer using engineered P1-based phagemids. ACS Synth Biol. 2012 Dec 21;1(12):583-9. doi: 10.1021/sb300054p. Epub 2012 Aug 30.
BBa_J72113-BBa_J72152Phagemid + GFPBacterial Expression, Synthetic Biology Anderson Scalable plasmid transfer using engineered P1-based phagemids. ACS Synth Biol. 2012 Dec 21;1(12):583-9. doi: 10.1021/sb300054p. Epub 2012 Aug 30.
BBa_J72113-BBa_J72103AraC-pBad + coi + cin + repL + pacABacterial Expression, Synthetic Biology Anderson Scalable plasmid transfer using engineered P1-based phagemids. ACS Synth Biol. 2012 Dec 21;1(12):583-9. doi: 10.1021/sb300054p. Epub 2012 Aug 30.
pCsy4csy4 geneBacterial Expression, Synthetic Biology Arkin RNA processing enables predictable programming of gene expression. Nat Biotechnol. 2012 Oct;30(10):1002-6. doi: 10.1038/nbt.2355. Epub 2012 Sep 16.
pZE-PalkB-GFPGFP, PalkBBacterial Expression, Synthetic Biology Alper Evolution of an alkane-inducible biosensor for increased responsiveness to short-chain alkanes. J Biotechnol. 2012 Apr 15;158(3):75-9. doi: 10.1016/j.jbiotec.2012.01.028. Epub 2012 Feb 2.
pACYC-PLlambda-alkS(WT)AlkS(WT)Bacterial Expression, Synthetic Biology Alper Evolution of an alkane-inducible biosensor for increased responsiveness to short-chain alkanes. J Biotechnol. 2012 Apr 15;158(3):75-9. doi: 10.1016/j.jbiotec.2012.01.028. Epub 2012 Feb 2.
pACYC-PLlambda-alkSm4-3AlkSm4-3 (Other)Synthetic Biology Alper Evolution of an alkane-inducible biosensor for increased responsiveness to short-chain alkanes. J Biotechnol. 2012 Apr 15;158(3):75-9. doi: 10.1016/j.jbiotec.2012.01.028. Epub 2012 Feb 2.
AND-gate-V2.0AND-gateBacterial Expression, Synthetic Biology Endy Amplifying genetic logic gates. Science. 2013 May 3;340(6132):599-603. doi: 10.1126/science.1232758. Epub 2013 Mar 28.
OR gateOR-gateBacterial Expression, Synthetic Biology Endy Amplifying genetic logic gates. Science. 2013 May 3;340(6132):599-603. doi: 10.1126/science.1232758. Epub 2013 Mar 28.
NOR gate-V2.0NOR-gateBacterial Expression, Synthetic Biology Endy Amplifying genetic logic gates. Science. 2013 May 3;340(6132):599-603. doi: 10.1126/science.1232758. Epub 2013 Mar 28.
XOR gate-V2.0XOR-gateBacterial Expression, Synthetic Biology Endy Amplifying genetic logic gates. Science. 2013 May 3;340(6132):599-603. doi: 10.1126/science.1232758. Epub 2013 Mar 28.
XNOR-gate-V2.0XNOR-gateBacterial Expression, Synthetic Biology Endy Amplifying genetic logic gates. Science. 2013 May 3;340(6132):599-603. doi: 10.1126/science.1232758. Epub 2013 Mar 28.
XNOR-gate-V2.0-ASVXNOR-gateBacterial Expression, Synthetic Biology Endy Amplifying genetic logic gates. Science. 2013 May 3;340(6132):599-603. doi: 10.1126/science.1232758. Epub 2013 Mar 28.
Dual-recombinase-controllerpTEt-Bxb1, pBAD-TP901Synthetic Biology Endy Amplifying genetic logic gates. Science. 2013 May 3;340(6132):599-603. doi: 10.1126/science.1232758. Epub 2013 Mar 28.
PLlacO-1-mfLonLonBacterial Expression, Synthetic Biology Maharbiz A genetic bistable switch utilizing nonlinear protein degradation. J Biol Eng. 2012 Jul 9;6(1):9.
pBbA5k-EPL123AcrABBacterial Expression, Synthetic Biology Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL3mexF, Avin_33870 (Synthetic)Bacterial Expression, Synthetic Biology Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL4Avin_44060 (Synthetic)Bacterial Expression, Synthetic Biology Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL11ttgB, PP_1385 (Synthetic)Synthetic Biology Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL12PP_3456 (Synthetic)Synthetic Biology Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL14mexF, PP_3426 (Synthetic)Synthetic Biology Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL15PP_0906 (Synthetic)Synthetic Biology Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL33PA_2018 (Synthetic)Synthetic Biology Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL36mexF, PA2494 (Synthetic)Synthetic Biology Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL37PA_4375 (Synthetic)Synthetic Biology Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL42PFL_1331 (Synthetic)Synthetic Biology Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL43PFL_3271 (Synthetic)Synthetic Biology Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL45PFL_1028 (Synthetic)Synthetic Biology Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL48PFL_1158 (Synthetic)Synthetic Biology Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL52Rmet_4866 (Synthetic)Synthetic Biology Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL54Rmet_1702 (Synthetic)Synthetic Biology Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL55Maqu_3494 (Synthetic)Synthetic Biology Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL56Maqu_0582 (Synthetic)Synthetic Biology Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL59PSPPH_4014 (Synthetic)Synthetic Biology Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL61PSPPH_2907 (Synthetic)Synthetic Biology Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL63mdtB, PSPPH_2641 (Synthetic)Synthetic Biology Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL64PSPPH_0733 (Synthetic)Synthetic Biology Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL65PSPPH_1196 (Synthetic)Synthetic Biology Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL66PSPPH_0329Synthetic Biology Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL68Gmet_1664 (Synthetic)Synthetic Biology Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL69Gmet_2518 (Synthetic)Synthetic Biology Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL70Gmet_1652 (Synthetic)Synthetic Biology Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL84BP_2076 (Synthetic)Synthetic Biology Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL90DVU_0438 (Synthetic)Synthetic Biology Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL95AcrB/AcrD/AcrFa, ABO_0964 (Synthetic)Synthetic Biology Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL98acrB, PSHAb_0324 (Synthetic)Synthetic Biology Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL100Shal_0198 (Synthetic)Synthetic Biology Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL101Shal_1195 (Synthetic)Synthetic Biology Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL102Shal_2167 (Synthetic)Synthetic Biology Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL122acrF (Synthetic)Synthetic Biology Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL124acrD (Synthetic)Synthetic Biology Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
BB-­NIC-­II-­HisN-­TET12TET12 (Synthetic)Bacterial Expression, Synthetic Biology Jerala Design of a single-chain polypeptide tetrahedron assembled from coiled-coil segments. Nat Chem Biol. 2013 Apr 28. doi: 10.1038/nchembio.1248.
pBEST-PLtetO-1-UTR1-TetR-deGFP-T500tetR-deGFP (Synthetic)Bacterial Expression, Synthetic Biology Murray Murray Lab pBEST plasmids (unpublished)
pBEST-PLtetO-1-UTR1-LacI-YbaQ-T500Lac repressorBacterial Expression, Synthetic Biology Murray Murray Lab pBEST plasmids (unpublished)
pBEST-PLlacO-1-UTR1-deCFP-T500deCFP (Synthetic)Bacterial Expression, Synthetic Biology Murray Murray Lab pBEST plasmids (unpublished)
pBEST-PLlacO-1-UTR1-TetR-YbaQ-T500Tetracycline repressorBacterial Expression, Synthetic Biology Murray Murray Lab pBEST plasmids (unpublished)
pBEST-p15a-OR2-OR1-Pr-UTR1-sigma28-T500sigma factor 28Bacterial Expression, Synthetic Biology Murray Murray Lab pBEST plasmids (unpublished)
pBEST-pTar-UTR1-TetR-T500Tetracycline repressorBacterial Expression, Synthetic Biology Murray Murray Lab pBEST plasmids (unpublished)
pBEST-p15a-PLtetO-1-UTR1-LacI-T500Lac repressorBacterial Expression, Synthetic Biology Murray Murray Lab pBEST plasmids (unpublished)
pBEST-OR2-OR1-Pr-UTR1-araC-T500araCBacterial Expression, Synthetic Biology Murray Murray Lab pBEST plasmids (unpublished)
pBEST-p15a-pBAD-UTR1-TetR-T500Tetracycline repressorBacterial Expression, Synthetic Biology Murray Murray Lab pBEST plasmids (unpublished)
pBEST-p15a-pBAD-TetO1-UTR1-deGFP-ssrA-T500deGFP (Synthetic)Bacterial Expression, Synthetic Biology Murray Murray Lab pBEST plasmids (unpublished)
pBEST-OR2-OR1-Pr-araC, pBAD-TetR, pBAD-TetO1-deGFP-ssrAdeGFP, Tetracycline repressor, araCBacterial Expression, Synthetic Biology Murray Murray Lab pBEST plasmids (unpublished)
pBADmod1-linker2-gamSgamS (Other)Bacterial Expression, Synthetic Biology Murray Murray Lab gamS and ClpX plasmids (unpublished)
pE-FLPflpBacterial Expression, Synthetic Biology Endy One-step cloning and chromosomal integration of DNA. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
pOSIP-CH (CamR, HK022)Bacterial Expression, Synthetic Biology Endy One-step cloning and chromosomal integration of DNA. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
pOSIP-CT (CamR, P21)Bacterial Expression, Synthetic Biology Endy One-step cloning and chromosomal integration of DNA. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
pOSIP-KC (KanR, PhiC31)Bacterial Expression, Synthetic Biology Endy One-step cloning and chromosomal integration of DNA. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
pOSIP-KH (KanR, HK022)Bacterial Expression, Synthetic Biology Endy One-step cloning and chromosomal integration of DNA. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
pOSIP-KL (KanR, Lambda)Bacterial Expression, Synthetic Biology Endy One-step cloning and chromosomal integration of DNA. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
pOSIP-KO (KanR, 186)Bacterial Expression, Synthetic Biology Endy One-step cloning and chromosomal integration of DNA. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
pOSIP-KP (KanR, Phi80)Bacterial Expression, Synthetic Biology Endy One-step cloning and chromosomal integration of DNA. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
pOSIP-KT (KanR, P21)Bacterial Expression, Synthetic Biology Endy One-step cloning and chromosomal integration of DNA. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
pOSIP-TH (TetR, HK022)Bacterial Expression, Synthetic Biology Endy One-step cloning and chromosomal integration of DNA. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
pOSIP-TT (TetR, P21)Bacterial Expression, Synthetic Biology Endy One-step cloning and chromosomal integration of DNA. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
pGR-BBa_B0010BBa_B0010 TerminatorBacterial Expression, Synthetic Biology Voigt Characterization of 582 natural and synthetic terminators and quantification of their design constraints. Nat Methods. 2013 Jun 2. doi: 10.1038/nmeth.2515.
pGR-L3S2P21L3S2P21 Terminator (Synthetic)Bacterial Expression, Synthetic Biology Voigt Characterization of 582 natural and synthetic terminators and quantification of their design constraints. Nat Methods. 2013 Jun 2. doi: 10.1038/nmeth.2515.
pGR-L3S2P00L3S2P00 Terminator (Synthetic)Bacterial Expression, Synthetic Biology Voigt Characterization of 582 natural and synthetic terminators and quantification of their design constraints. Nat Methods. 2013 Jun 2. doi: 10.1038/nmeth.2515.
pGR-ECK120015170ECK120015170 TerminatorBacterial Expression, Synthetic Biology Voigt Characterization of 582 natural and synthetic terminators and quantification of their design constraints. Nat Methods. 2013 Jun 2. doi: 10.1038/nmeth.2515.
pGR-L3S2P36L3S2P36 Terminator (Synthetic)Bacterial Expression, Synthetic Biology Voigt Characterization of 582 natural and synthetic terminators and quantification of their design constraints. Nat Methods. 2013 Jun 2. doi: 10.1038/nmeth.2515.
pGR-ECK125108723ECK125108723 TerminatorBacterial Expression, Synthetic Biology Voigt Characterization of 582 natural and synthetic terminators and quantification of their design constraints. Nat Methods. 2013 Jun 2. doi: 10.1038/nmeth.2515.
pGR-ECK120010783ECK120010783 TerminatorBacterial Expression, Synthetic Biology Voigt Characterization of 582 natural and synthetic terminators and quantification of their design constraints. Nat Methods. 2013 Jun 2. doi: 10.1038/nmeth.2515.
pGR-ECK120010800ECK120010800 TerminatorBacterial Expression, Synthetic Biology Voigt Characterization of 582 natural and synthetic terminators and quantification of their design constraints. Nat Methods. 2013 Jun 2. doi: 10.1038/nmeth.2515.
pGR-ECK120033127ECK120033127 TerminatorBacterial Expression, Synthetic Biology Voigt Characterization of 582 natural and synthetic terminators and quantification of their design constraints. Nat Methods. 2013 Jun 2. doi: 10.1038/nmeth.2515.
pJBEI-6409BglBrick plasmid (=pBbA5c-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coliCodon-optimized sequences for MEV pathway expression in E. coli to produce limonene (Saccharomyces cerevisiae)Bacterial Expression, Synthetic Biology ; BglBrick Lee Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production. Metab Eng. 2013 May 29;19C:33-41. doi: 10.1016/j.ymben.2013.05.004.
pJBEI-6410BglBrick plasmid (=pBbA5a-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coliCodon-optimized sequences for MEV pathway expression in E. coli to produce limonene (Saccharomyces cerevisiae)Bacterial Expression, Synthetic Biology ; BglBrick Lee Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production. Metab Eng. 2013 May 29;19C:33-41. doi: 10.1016/j.ymben.2013.05.004.
pJBEI-6411BglBrick plasmid (=pBbB8k-P450) coding for a cytochrome P450 and its associated reductases to oxidize limonene to perillyl alcohol in E. coliahpG-ahpH-ahpI coding for cytochrome P450 (CYP153A6) and its associated ferredoxin and ferredoxin reductase (Other)Bacterial Expression, Synthetic Biology ; BglBrick Lee Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production. Metab Eng. 2013 May 29;19C:33-41. doi: 10.1016/j.ymben.2013.05.004.
pCS-PesaRluxwt esaR promoter in pCS26PesaR (Other)Bacterial Expression, Synthetic Biology Collins Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity. ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6.
pCS-PluxIluxwt luxI promoter in pCS26PluxI (Other)Bacterial Expression, Synthetic Biology Collins Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity. ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6.
pAC-EsaRwt esaR gene under control of Plac in pACYC184Plac-esaR-terminatorBacterial Expression, Synthetic Biology Collins Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity. ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6.
pAC-EsaR-I70V/D91GpAC-EsaR with a208g and a272g mutations in esaResaR-I70V/D91GBacterial Expression, Synthetic Biology Collins Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity. ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6.
pAC-EsaR-V220ApAC-EsaR with a c659t mutation in esaResaR-V220ABacterial Expression, Synthetic Biology Collins Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity. ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6.
pAC-EsaR-D91GpAC-EsaR with a a272g mutation in esaResaR-D91GBacterial Expression, Synthetic Biology Collins Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity. ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6.
pAC-EsaR-I70V/D91G/V220ApAC-EsaR with a208g, a272g, c659t mutations in esaResaR-I70V/D91G/V220ABacterial Expression, Synthetic Biology Collins Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity. ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6.
pAC-EsaR-D91G/V220ApAC-EsaR with a272g and c659t mutations in esaResaR-D91G/V220ABacterial Expression, Synthetic Biology Collins Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity. ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6.
pCS-PesaR-DluxPesaR-D promoter in pCS26PesaR-DBacterial Expression, Synthetic Biology Collins Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression. ACS Synth Biol. 2013 Jul 23.
pCS-PesaR-PluxPesaR-P promoter in pCS26PesaR-PBacterial Expression, Synthetic Biology Collins Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression. ACS Synth Biol. 2013 Jul 23.
pET-PesaR-DluxPesaR-D promoter, luxCDABE, and terminator in pET17b (AmpR)PesaR-DBacterial Expression, Synthetic Biology Collins Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression. ACS Synth Biol. 2013 Jul 23.
pET-PesaR-CluxPesaR-C promoter, luxCDABE, and terminator in pET17b(AmpR)PesaR-CBacterial Expression, Synthetic Biology Collins Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression. ACS Synth Biol. 2013 Jul 23.
pET-PesaR-PluxPesaR-P promoter, luxCDABE, and terminator in pET17b (AmpR)PesaR-PBacterial Expression, Synthetic Biology Collins Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression. ACS Synth Biol. 2013 Jul 23.
pAC-EsaR-EsaIesaI gene downstream of esaR in pAC-EsaR (CmR)esaI-termBacterial Expression, Synthetic Biology Collins Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression. ACS Synth Biol. 2013 Jul 23.
pAC-EsaR-I70V-EsaIesaI gene downstream of esaR-a208g in pAC-EsaR-I70V (CmR)esaI-termBacterial Expression, Synthetic Biology Collins Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression. ACS Synth Biol. 2013 Jul 23.
pAC-EsaR-D91G-EsaIesaI gene downstream of esaR-a272g in pAC-EsaR-D91G (CmR)esaI-termBacterial Expression, Synthetic Biology Collins Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression. ACS Synth Biol. 2013 Jul 23.
pAC-EsaR-I70VpAC-EsaR with a208g mutation in esaR esaR-I70VBacterial Expression, Synthetic Biology Collins Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity. ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6.
pET-PesaRluxPesaR promoter, luxCDABE, and terminator in pET17b (AmpR)PesaR-luxCDABE-termBacterial Expression, Synthetic Biology Collins Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression. ACS Synth Biol. 2013 Jul 23.
pFAB3905BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD1 efficiency.promoter 14 and BCD1 (Synthetic)Bacterial Expression, Synthetic Biology Endy Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
pFAB3906BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD2 efficiency.promoter 14 and BCD2 (Synthetic)Bacterial Expression, Synthetic Biology Endy Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
pFAB3909BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD5 efficiency.promoter 14 and BCD5 (Synthetic)Bacterial Expression, Synthetic Biology Endy Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
pFAB3910BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD6 efficiency.promoter 14 and BCD6 (Synthetic)Bacterial Expression, Synthetic Biology Endy Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
pFAB3911BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD7 efficiency.promoter 14 and BCD7 (Synthetic)Bacterial Expression, Synthetic Biology Endy Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
pFAB3912BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD8 efficiency.promoter 14 and BCD8 (Synthetic)Bacterial Expression, Synthetic Biology Endy Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
pFAB3913BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD9 efficiency.promoter 14 and BCD9 (Synthetic)Bacterial Expression, Synthetic Biology Endy Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
pFAB3914BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD10 efficiency.promoter 14 and BCD10 (Synthetic)Bacterial Expression, Synthetic Biology Endy Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
pFAB3915BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD11 efficiency.promoter 14 and BCD11 (Synthetic)Bacterial Expression, Synthetic Biology Endy Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
pFAB3916BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD12 efficiency.promoter 14 and BCD12 (Synthetic)Bacterial Expression, Synthetic Biology Endy Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
pFAB3989BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD13 efficiency.promoter 14 and BCD13 (Synthetic)Bacterial Expression, Synthetic Biology Endy Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
pFAB3991BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD15 efficiency.promoter 14 and BCD15 (Synthetic)Bacterial Expression, Synthetic Biology Endy Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
pFAB3992BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD16 efficiency.promoter 14 and BCD16 (Synthetic)Bacterial Expression, Synthetic Biology Endy Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
pFAB3993BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD17 efficiency.promoter 14 and BCD17 (Synthetic)Bacterial Expression, Synthetic Biology Endy Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
pFAB3994BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD18 efficiency.promoter 14 and BCD18 (Synthetic)Bacterial Expression, Synthetic Biology Endy Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
pFAB3995BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD19 efficiency.promoter 14 and BCD19 (Synthetic)Bacterial Expression, Synthetic Biology Endy Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
pFAB3996BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD20 efficiency.promoter 14 and BCD20 (Synthetic)Bacterial Expression, Synthetic Biology Endy Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
pFAB3997BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD21 efficiency.promoter 14 and BCD21 (Synthetic)Bacterial Expression, Synthetic Biology Endy Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
pFAB3998BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD22 efficiency.promoter 14 and BCD22 (Synthetic)Bacterial Expression, Synthetic Biology Endy Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
pFAB3999BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD23 efficiency.promoter 14 and BCD23 (Synthetic)Bacterial Expression, Synthetic Biology Endy Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
pFAB4000BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD24 efficiency.promoter 14 and BCD24 (Synthetic)Bacterial Expression, Synthetic Biology Endy Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
pFAB3665BIOFAB RFP reporter plasmid for measuring promoter 1 + BCD1 efficiency.promoter 1 and BCD1 (Synthetic)Bacterial Expression, Synthetic Biology Endy Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
pFAB3677BIOFAB RFP reporter plasmid for measuring promoter 2 + BCD1 efficiency.promoter 2 and BCD1 (Synthetic)Bacterial Expression, Synthetic Biology Endy Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
pFAB3689BIOFAB RFP reporter plasmid for measuring promoter 3 + BCD1 efficiency.promoter 3 and BCD1 (Synthetic)Bacterial Expression, Synthetic Biology Endy Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
pFAB3701BIOFAB RFP reporter plasmid for measuring promoter 4 + BCD1 efficiency.promoter 4 and BCD1 (Synthetic)Bacterial Expression, Synthetic Biology Endy Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
pFAB3713BIOFAB RFP reporter plasmid for measuring promoter 5 + BCD1 efficiency.promoter 5 and BCD1 (Synthetic)Bacterial Expression, Synthetic Biology Endy Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
pFAB3725BIOFAB RFP reporter plasmid for measuring promoter 6 + BCD1 efficiency.promoter 6 and BCD1 (Synthetic)Bacterial Expression, Synthetic Biology Endy Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
pFAB3737BIOFAB RFP reporter plasmid for measuring promoter 7 + BCD1 efficiency.promoter 7 and BCD1 (Synthetic)Bacterial Expression, Synthetic Biology Endy Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
pFAB3833BIOFAB RFP reporter plasmid for measuring promoter 8 + BCD1 efficiency.promoter 8 and BCD1 (Synthetic)Bacterial Expression, Synthetic Biology Endy Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
pFAB3845BIOFAB RFP reporter plasmid for measuring promoter 9 + BCD1 efficiency.promoter 9 and BCD1 (Synthetic)Bacterial Expression, Synthetic Biology Endy Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
pFAB3857BIOFAB RFP reporter plasmid for measuring promoter 10 + BCD1 efficiency.promoter 10 and BCD1 (Synthetic)Bacterial Expression, Synthetic Biology Endy Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
pFAB3869BIOFAB RFP reporter plasmid for measuring promoter 11 + BCD1 efficiency.promoter 11 and BCD1 (Synthetic)Bacterial Expression, Synthetic Biology Endy Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
pFAB3881BIOFAB RFP reporter plasmid for measuring promoter 12 + BCD1 efficiency.promoter 12 and BCD1 (Synthetic)Bacterial Expression, Synthetic Biology Endy Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
pFAB3893BIOFAB RFP reporter plasmid for measuring promoter 13 + BCD1 efficiency.promoter 13 and BCD1 (Synthetic)Bacterial Expression, Synthetic Biology Endy Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
pFAB801BIOFAB reporter plasmid for measuring trp_att_L126 termination efficiencytrp_att_L126 terminator (Synthetic)Bacterial Expression, Synthetic Biology Endy Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
pFAB803BIOFAB reporter plasmid for measuring T7 early termination efficiencyT7 early terminator (Synthetic)Bacterial Expression, Synthetic Biology Endy Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
pFAB804BIOFAB reporter plasmid for measuring T3 early termination efficiencyT3 early terminator (Synthetic)Bacterial Expression, Synthetic Biology Endy Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
pFAB806BIOFAB reporter plasmid for measuring lambda tR2 termination efficiencylambda tR2 terminator (Synthetic)Bacterial Expression, Synthetic Biology Endy Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
pFAB805BIOFAB reporter plasmid for measuring tonB termination efficiencytonB terminator (Synthetic)Bacterial Expression, Synthetic Biology Endy Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
pFAB816BIOFAB reporter plasmid for measuring ilvGEDA termination efficiencyilvGEDA (Synthetic)Bacterial Expression, Synthetic Biology Endy Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
pFAB807BIOFAB reporter plasmid for measuring RNAI termination efficiencyRNAI terminator (Synthetic)Bacterial Expression, Synthetic Biology Endy Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
pFAB808BIOFAB reporter plasmid for measuring rrnB T1 termination efficiencyrrnB T1 terminator (Synthetic)Bacterial Expression, Synthetic Biology Endy Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
pFAB815BIOFAB reporter plasmid for measuring rnpB T1 termination efficiencyrnpB T1 terminator (Synthetic)Bacterial Expression, Synthetic Biology Endy Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
pFAB820BIOFAB reporter plasmid for measuring tetAC termination efficiencytetAC terminator (Synthetic)Bacterial Expression, Synthetic Biology Endy Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
pFAB822BIOFAB reporter plasmid for measuring BBa_B1006 termination efficiencyBBa_B1006 terminator (Synthetic)Bacterial Expression, Synthetic Biology Endy Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
pFAB1907BIOFAB reporter plasmid for measuring BBa_B1006 U10 termination efficiencyBBa_B1006 U10 (Synthetic)Bacterial Expression, Synthetic Biology Endy Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
pFAB1902BIOFAB reporter plasmid for measuring M13_central_T_linker_rrnD_T1 termination efficiencyM13_central_T_linker_rrnD_T1 double terminator (Synthetic)Bacterial Expression, Synthetic Biology Endy Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
pFAB1910BIOFAB reporter plasmid for measuring rpoC termination efficiencyrpoC terminator (Synthetic)Bacterial Expression, Synthetic Biology Endy Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
pFAB1935BIOFAB reporter plasmid for measuring his_T_min_linker_crp_T_min termination efficiencyhis_T_min_linker_crp_T_min double terminator (Synthetic)Bacterial Expression, Synthetic Biology Endy Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
pRBBm34Expression of the gfp gene under control of the native xylose inducible promoter of Bacillus megateriumgfpBacterial Expression, Synthetic Biology Jahn A sucrose-inducible promoter system for the intra- and extracellular protein production in Bacillus megaterium. J Biotechnol. 2007 Dec 1;132(4):426-30. Epub 2007 Jul 10.
pRBBm59Shuttle vector E. coli/B.meg.promoter sacB - gfpBacterial Expression, Synthetic Biology Jahn A sucrose-inducible promoter system for the intra- and extracellular protein production in Bacillus megaterium. J Biotechnol. 2007 Dec 1;132(4):426-30. Epub 2007 Jul 10.
pRBBm69Shuttle vector E. coli/B.meg.promoter sacB - sacBBacterial Expression, Synthetic Biology Jahn A sucrose-inducible promoter system for the intra- and extracellular protein production in Bacillus megaterium. J Biotechnol. 2007 Dec 1;132(4):426-30. Epub 2007 Jul 10.
pRBBm76Shuttle vector E. coli/B.meg.promoter sacB - gfpBacterial Expression, Synthetic Biology Jahn A sucrose-inducible promoter system for the intra- and extracellular protein production in Bacillus megaterium. J Biotechnol. 2007 Dec 1;132(4):426-30. Epub 2007 Jul 10.
pSPYngK-hpShuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein YngK under control of the xylose inducible promoter signal peptide of YngKBacterial Expression, Synthetic Biology Jahn High-yield intra- and extracellular protein production using Bacillus megaterium. Appl Environ Microbiol. 2010 Jun;76(12):4037-46. doi: 10.1128/AEM.00431-10. Epub 2010 Apr 30.
pSPVpr-hp Shuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein Vpr under control of the xylose inducible promotersignal peptide of VprBacterial Expression, Synthetic Biology Jahn High-yield intra- and extracellular protein production using Bacillus megaterium. Appl Environ Microbiol. 2010 Jun;76(12):4037-46. doi: 10.1128/AEM.00431-10. Epub 2010 Apr 30.
pSPNprM-hpShuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein NprM under control of the xylose inducible promoter signal peptide of NprMBacterial Expression, Synthetic Biology Jahn High-yield intra- and extracellular protein production using Bacillus megaterium. Appl Environ Microbiol. 2010 Jun;76(12):4037-46. doi: 10.1128/AEM.00431-10. Epub 2010 Apr 30.
pSPYngK+-hpShuttle vector E. coli/B.meg.; carrying the codon optimized sequence of the signal peptide for protein secretion of the Bacillus megaterium protein YngK under control of the xylose inducible promotercodon optimized signal peptide of YngKBacterial Expression, Synthetic Biology Jahn High-yield intra- and extracellular protein production using Bacillus megaterium. Appl Environ Microbiol. 2010 Jun;76(12):4037-46. doi: 10.1128/AEM.00431-10. Epub 2010 Apr 30.
pSPAsp-hpShuttle vector E. coli/B.meg.; encoding the predicted optimal signal peptide for protein secretion using Bacillus megaterium under control of the xylose inducible promoter predicted optimal signal peptide for Bacillus megaterium protein secretionBacterial Expression, Synthetic Biology Jahn High-yield intra- and extracellular protein production using Bacillus megaterium. Appl Environ Microbiol. 2010 Jun;76(12):4037-46. doi: 10.1128/AEM.00431-10. Epub 2010 Apr 30.
pSPPac-hpShuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein Pac under control of the xylose inducible promotersignal peptide of PacBacterial Expression, Synthetic Biology Jahn High-yield intra- and extracellular protein production using Bacillus megaterium. Appl Environ Microbiol. 2010 Jun;76(12):4037-46. doi: 10.1128/AEM.00431-10. Epub 2010 Apr 30.
pPSP6+t-gfpShuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-SP6-inducible promotergfpBacterial Expression, Synthetic Biology Jahn Application of Escherichia coli phage K1E DNA-dependent RNA polymerase for in vitro RNA synthesis and in vivo protein production in Bacillus megaterium. Appl Microbiol Biotechnol. 2010 Sep;88(2):529-39. doi: 10.1007/s00253-010-2732-y. Epub 2010 Jul 2.
pPK1E-1+t-gfpShuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promotergfpBacterial Expression, Synthetic Biology Jahn Application of Escherichia coli phage K1E DNA-dependent RNA polymerase for in vitro RNA synthesis and in vivo protein production in Bacillus megaterium. Appl Microbiol Biotechnol. 2010 Sep;88(2):529-39. doi: 10.1007/s00253-010-2732-y. Epub 2010 Jul 2.
pPK1E-2+t-gfpShuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promotergfpBacterial Expression, Synthetic Biology Jahn Application of Escherichia coli phage K1E DNA-dependent RNA polymerase for in vitro RNA synthesis and in vivo protein production in Bacillus megaterium. Appl Microbiol Biotechnol. 2010 Sep;88(2):529-39. doi: 10.1007/s00253-010-2732-y. Epub 2010 Jul 2.
pPK1E-1-gfpShuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promotergfpBacterial Expression, Synthetic Biology Jahn Application of Escherichia coli phage K1E DNA-dependent RNA polymerase for in vitro RNA synthesis and in vivo protein production in Bacillus megaterium. Appl Microbiol Biotechnol. 2010 Sep;88(2):529-39. doi: 10.1007/s00253-010-2732-y. Epub 2010 Jul 2.
pHBintTShuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker tetracycline for B. megaterium, integration vectorBacterial Expression, Synthetic Biology Jahn Metabolic engineering of cobalamin (vitamin B12) production in Bacillus megaterium. Microb Biotechnol. 2010 Jan;3(1):24-37. doi: 10.1111/j.1751-7915.2009.00125.x. Epub 2009 Jun 10.
pHBintNShuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker neomycine for B. megaterium, integration vector Bacterial Expression, Synthetic Biology Jahn Metabolic engineering of cobalamin (vitamin B12) production in Bacillus megaterium. Microb Biotechnol. 2010 Jan;3(1):24-37. doi: 10.1111/j.1751-7915.2009.00125.x. Epub 2009 Jun 10.
pHBintEShuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker erytromycine for B. megaterium, integration vector Bacterial Expression, Synthetic Biology Jahn Metabolic engineering of cobalamin (vitamin B12) production in Bacillus megaterium. Microb Biotechnol. 2010 Jan;3(1):24-37. doi: 10.1111/j.1751-7915.2009.00125.x. Epub 2009 Jun 10.
pSP6-RNAPShuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage SP6 under control of the xylose inducible promoter PxylArna polymerase SP6Bacterial Expression, Synthetic Biology Jahn Application of Escherichia coli phage K1E DNA-dependent RNA polymerase for in vitro RNA synthesis and in vivo protein production in Bacillus megaterium. Appl Microbiol Biotechnol. 2010 Sep;88(2):529-39. doi: 10.1007/s00253-010-2732-y. Epub 2010 Jul 2.
pK1E-RNAPShuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage K1E under control of the xylose inducible promoter PxylArna polymerase K1EBacterial Expression, Synthetic Biology Jahn Application of Escherichia coli phage K1E DNA-dependent RNA polymerase for in vitro RNA synthesis and in vivo protein production in Bacillus megaterium. Appl Microbiol Biotechnol. 2010 Sep;88(2):529-39. doi: 10.1007/s00253-010-2732-y. Epub 2010 Jul 2.
pPK1E-1Shuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage K1EBacterial Expression, Synthetic Biology Jahn Application of Escherichia coli phage K1E DNA-dependent RNA polymerase for in vitro RNA synthesis and in vivo protein production in Bacillus megaterium. Appl Microbiol Biotechnol. 2010 Sep;88(2):529-39. doi: 10.1007/s00253-010-2732-y. Epub 2010 Jul 2.
pPK1E-2Shuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage K1EBacterial Expression, Synthetic Biology Jahn Application of Escherichia coli phage K1E DNA-dependent RNA polymerase for in vitro RNA synthesis and in vivo protein production in Bacillus megaterium. Appl Microbiol Biotechnol. 2010 Sep;88(2):529-39. doi: 10.1007/s00253-010-2732-y. Epub 2010 Jul 2.
pPK1E-1+tShuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage K1EBacterial Expression, Synthetic Biology Jahn Application of Escherichia coli phage K1E DNA-dependent RNA polymerase for in vitro RNA synthesis and in vivo protein production in Bacillus megaterium. Appl Microbiol Biotechnol. 2010 Sep;88(2):529-39. doi: 10.1007/s00253-010-2732-y. Epub 2010 Jul 2.
pPK1E-2+tShuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage K1EBacterial Expression, Synthetic Biology Jahn Application of Escherichia coli phage K1E DNA-dependent RNA polymerase for in vitro RNA synthesis and in vivo protein production in Bacillus megaterium. Appl Microbiol Biotechnol. 2010 Sep;88(2):529-39. doi: 10.1007/s00253-010-2732-y. Epub 2010 Jul 2.
pPSP6+tShuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage SP6Bacterial Expression, Synthetic Biology Jahn Application of Escherichia coli phage K1E DNA-dependent RNA polymerase for in vitro RNA synthesis and in vivo protein production in Bacillus megaterium. Appl Microbiol Biotechnol. 2010 Sep;88(2):529-39. doi: 10.1007/s00253-010-2732-y. Epub 2010 Jul 2.
pPSP6Shuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage SP6Bacterial Expression, Synthetic Biology Jahn Application of Escherichia coli phage K1E DNA-dependent RNA polymerase for in vitro RNA synthesis and in vivo protein production in Bacillus megaterium. Appl Microbiol Biotechnol. 2010 Sep;88(2):529-39. doi: 10.1007/s00253-010-2732-y. Epub 2010 Jul 2.
pJS167Positive feedback plasmid from oscillatoraraC gene with ssrA tag, monomeric yeast-enhanced green fluorescent proteinBacterial Expression, Synthetic Biology Hasty A fast, robust and tunable synthetic gene oscillator. Nature. 2008 Nov 27;456(7221):516-9. doi: 10.1038/nature07389. Epub 2008 Oct 29.
pJS169Negative feedback plasmid from oscillatorlacI with ssrA tagBacterial Expression, Synthetic Biology Hasty A fast, robust and tunable synthetic gene oscillator. Nature. 2008 Nov 27;456(7221):516-9. doi: 10.1038/nature07389. Epub 2008 Oct 29.
pTD103aiiANegative feedback plasmid from oscillatoraiiA and LuxRBacterial Expression, Synthetic Biology Hasty A synchronized quorum of genetic clocks. Nature. 2010 Jan 21;463(7279):326-30. doi: 10.1038/nature08753.
pTD103luxI_sfGFPPositive feedback plasmid from oscillatorLuxI, sfGFP, and LuxRBacterial Expression, Synthetic Biology Hasty A sensing array of radically coupled genetic 'biopixels'. Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722.
pTD103aiiA(Cm)Negative feedback plasmid from oscillatoraiiA and LuxRBacterial Expression, Synthetic Biology Hasty A sensing array of radically coupled genetic 'biopixels'. Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722.
pTD103luxI_sfGFP -RPositive feedback plasmid from oscillator without luxRsfGFP and luxIBacterial Expression, Synthetic Biology Hasty A sensing array of radically coupled genetic 'biopixels'. Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722.
pTD103aiiA(Cm) -RNegative feedback plasmid from oscillator without luxRaiiABacterial Expression, Synthetic Biology Hasty A sensing array of radically coupled genetic 'biopixels'. Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722.
pZSm45 ndhIILux-regulated expression of NDH-2 enzymendhII and LuxRBacterial Expression, Synthetic Biology Hasty A sensing array of radically coupled genetic 'biopixels'. Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722.
pZSm45 sodALux-regulated expression of sodA enzymesodA LuxRBacterial Expression, Synthetic Biology Hasty A sensing array of radically coupled genetic 'biopixels'. Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722.
pZSm47a luxR RBSArsR-regulated expression of luxRluxRBacterial Expression, Synthetic Biology Hasty A sensing array of radically coupled genetic 'biopixels'. Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722.
pZS47a luxI-laa RBSArsR-regulated expression of luxI-laaLuxIBacterial Expression, Synthetic Biology Hasty A sensing array of radically coupled genetic 'biopixels'. Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722.
pRF-AmtRAmtR repressor (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pRF-AmeRAmeR repressor (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pRF-BetIBetI repressor (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pRF-BM3R1BM3R1 repressor (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pRF-ButRButR repressor (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pRF-HapRHapR repressor (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pRF-HlyIIRHlyIIR repressor (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pRF-IcaRIcaR repressor (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pRF-LitRLitR repressor (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pRF-LmrALmrA repressor (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pRF-McbRMcbR repressor (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pRF-Orf2Orf2 repressor (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pRF-PhlFPhlF repressor (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pRF-PsrAPsrA repressor (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pRF-QacRQacR repressor (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pRF-ScbRScbR repressor (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pRF-SmcRSmcR repressor (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pRF-SrpRSrpR repressor (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pRF-TarATarA repressor (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pRF-TetRTetR repressor (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pLux-LmrALmrA repressor (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pNAND-PhlFPhlF repressor, pPhlF-pLmrA reporter (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pANDExpression of AND gate in bacteriaAND gate (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pVRa01_3473ECF01_3473 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRa02_915ECF02_915 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRa02_2817ECF02_2817 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRa03_1198ECF03_1198 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRa03_1244ECF03_1244 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRa04_1609ECF04_1609 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRa04_1617ECF04_1617 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRa07_980ECF07_980 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRa07_1134ECF07_1134 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRa11_987ECF11_987 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRa11_3726ECF11_3726 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRa12_807ECF12_807 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRa12_808ECF12_808 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRa14_1324ECF14_1324 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRa14_3200ECF14_3200 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRa15_436ECF15_436 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRa15_524ECF15_524 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRa16_973ECF16_973 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRa16_3622ECF16_3622 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRa17_1458ECF17_1458 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRa17_1691ECF17_1691 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRa20_992ECF20_992 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRa20_2913ECF20_2913 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRa22_1147ECF22_1147 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRa22_4450ECF22_4450 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRa25_1643ECF25_1643 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRa25_1645ECF25_1645 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRa26_837ECF26_837 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRa26_4464ECF26_4464 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRa27_1331ECF27_1331 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRa27_4265ECF27_4265 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRa28_1088ECF28_1088 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRa29_371ECF29_371 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRa30_35ECF30_35 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRa30_83ECF30_83 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRa31_34ECF31_34 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRa31_2963ECF31_2963 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRa32_1122ECF32_1122 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRa32_3724ECF32_3724 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRa33_375ECF33_375 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRa33_423ECF33_423 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRa34_1384ECF34_1384 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRa34_3302ECF34_3302 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRa35_3582ECF35_3582 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRa37_2513ECF37_2513 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRa37_3390ECF37_3390 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRa38_1322ECF38_1322 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRa38_1442ECF38_1442 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRa39_2973ECF39_2973 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRa40_3198ECF40_3198 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRa41_491ECF41_491 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRa42_4454ECF42_4454 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRb01_syn20sfgfp (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRb02_2817sfgfp (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRb03_up1198sfgfp (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRb11_3726sfgfp (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRb12_up807sfgfp (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRb15_up436sfgfp (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRb16_3622sfgfp (Synthetic)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRb17_up1691sfgfp (Synthetic)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRb18_up1700sfgfp (Synthetic)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRb19_up1315sfgfp (Synthetic)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRb20_992sfgfp (Synthetic)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRb21_up4014sfgfp (Synthetic)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRb22_up1147sfgfp (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRb25_up4311sfgfp (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRb26_up601sfgfp (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRb29_up371sfgfp (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRb30_2079sfgfp (Synthetic)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRb31_34sfgfp (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRb32_1122sfgfp (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRb33_375sfgfp (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRb38_up1322sfgfp (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRb39_up1413sfgfp (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRb41_up1141sfgfp (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRb42_up4062sfgfp (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRc01_3473AS01_3473 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRc02_915AS02_915 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRc03_1198AS03_1198 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRc03_1244AS03_1244 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRc04_1609AS04_1609 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRc11_987AS11_987 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRc14_1324AS14_1324 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRc15_436AS15_436 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRc15_524AS15_524 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRc16_973AS16_973 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRc16_3622AS16_3622 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRc20_992AS20_992 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRc22_1147AS22_1147 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRc22_4450AS22_4450 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRc25_1643_cl43AS25_1643_cl43 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRc25_1645AS25_1645 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRc27_1331AS27_1331 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRc27_4265AS27_4265 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRc30_35AS30_35 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRc31_34AS31_34 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRc33_375AS33_375 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRc33_423AS33_423 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRc35_3582AS35_3582 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRc37_2513AS37_2513 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pVRc38_1322AS38_1322 (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pN565expresses an attenuated T7 RNAP under an isopropyl β-d-1-thiogalactopyranoside (IPTG) inducible controlT7 RNAP* (Other)Bacterial Expression, Synthetic Biology Voigt Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
pBL4Expresses CcaS and CcaR constitutively, and sfGFP/TetR under the PcpcG2 promoterCcaS (Other), CcaR (Other)Bacterial Expression, Synthetic Biology Tabor Characterizing bacterial gene circuit dynamics with optically programmed gene expression signals. Nat Methods. 2014 Mar 9. doi: 10.1038/nmeth.2884.
pBL6Expresses mCherry under the PLtetO-1 promotermCherry (Synthetic)Bacterial Expression, Synthetic Biology Tabor Characterizing bacterial gene circuit dynamics with optically programmed gene expression signals. Nat Methods. 2014 Mar 9. doi: 10.1038/nmeth.2884.
pEO100cExpresses sfGFP under the PompC1157 promotersfGFP (Synthetic)Bacterial Expression, Synthetic Biology Tabor Characterizing bacterial gene circuit dynamics with optically programmed gene expression signals. Nat Methods. 2014 Mar 9. doi: 10.1038/nmeth.2884.
pJT119bExpresses CcaS and CcaR constitutively, and sfGFP under the PcpcG2 promoterCcaS (Other), CcaR (Other), sfGFP (Synthetic)Bacterial Expression, Synthetic Biology Tabor Characterizing bacterial gene circuit dynamics with optically programmed gene expression signals. Nat Methods. 2014 Mar 9. doi: 10.1038/nmeth.2884.
pCph8Expresses Cph8 under a modified PLtetO-1 promoterCph8 (Synthetic)Bacterial Expression, Synthetic Biology Tabor Characterizing bacterial gene circuit dynamics with optically programmed gene expression signals. Nat Methods. 2014 Mar 9. doi: 10.1038/nmeth.2884.
pS3Backbone: pBbB5c Origin Of Replication:pBBR1 Selection Markers: Chloramphenicol Promoters: PLacUV5aroE, aroD, aroB, aroG*, ppsA, tktABacterial Expression, Synthetic Biology Keasling Modular engineering of L-tyrosine production in Escherichia coli. Appl Environ Microbiol. 2012 Jan;78(1):89-98. doi: 10.1128/AEM.06017-11. Epub 2011 Oct 21.
pY1Backbone: pRBS-01, Origin Of Replication: p15a, Selection Markers: Amp, Promoters: PLacUV5,noneBacterial Expression, Synthetic Biology ; Empty Vector Keasling Modular engineering of L-tyrosine production in Escherichia coli. Appl Environ Microbiol. 2012 Jan;78(1):89-98. doi: 10.1128/AEM.06017-11. Epub 2011 Oct 21.
pTrc-trGPPS(CO)-LSpTrc-trGPPS(CO)-LS: A plasmid that has truncated codon optimized geranylpyrophosphate synthase and truncated limonene synthaseLimonene Synthase, ApR, LacIqBacterial Expression, Synthetic Biology Keasling Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production. Metab Eng. 2013 May 29;19C:33-41. doi: 10.1016/j.ymben.2013.05.004.
pY3Origin Of Replication: p15a, Selection Markers: Amp, Promoters: PLacUV5tyrB, tyrA, aroC, aroA, aroLBacterial Expression, Synthetic Biology Keasling Modular engineering of L-tyrosine production in Escherichia coli. Appl Environ Microbiol. 2012 Jan;78(1):89-98. doi: 10.1128/AEM.06017-11. Epub 2011 Oct 21.
pBBRBB-Ppuf1-1200-DsRedExpressed DsRed using the Ppuf1-1200 promoter segment, for use in R. sphaeroidesDsRedSynthetic Biology Schmidt-dannert BioBrick compatible vector system for protein expression in Rhodobacter sphaeroides. Appl Microbiol Biotechnol. 2014 Feb 9.
pBBRBB-Ppuf843-1200-DsRedExpressed DsRed using the Ppuf843-1200 promoter segment, for use in R. sphaeroides DsRedSynthetic Biology Schmidt-dannert BioBrick compatible vector system for protein expression in Rhodobacter sphaeroides. Appl Microbiol Biotechnol. 2014 Feb 9.
pBBRBB-Ppuf843-1200-PRExpresses proteorhodopsin using the Ppuf843-1200 promoter segment, for use in R. sphaeroides Proteorhodopsin (Other)Bacterial Expression, Synthetic Biology Schmidt-dannert BioBrick compatible vector system for protein expression in Rhodobacter sphaeroides. Appl Microbiol Biotechnol. 2014 Feb 9.
pET21a-AcrRAcrR (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pET21a-AmeRAmeR (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pET21a-AmrRAmrR (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pET21a-AmtRAmtR (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pET21a-BarABarA (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pET21a-BarBBarB (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pET21a-BetIBetI (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pET21a-BM1P1BM1P1 (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pET21a-BM3R1BM3R1 (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pET21a-BpeRBpeR (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pET21a-CalR1CalR1 (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pET21a-CampRCampR (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pET21a-CasRCasR (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pET21a-CprBCprB (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pET21a-Cyp106Cyp106 (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pET21a-DhaRDhaR (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pET21a-Ef0113Ef0113 (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pET21a-EthREthR (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pET21a-HapRHapR (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pET21a-HemRHemR (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pET21a-HlyIIRHlyIIR (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pET21a-IcaR-eIcaR (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pET21a-IfeRIfeR (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pET21a-JadR2JadR2 (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pET21a-KstRKstR (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pET21a-LitRLitR (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pET21a-LuxTLuxT (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pET21a-McbRMcbR (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pET21a-MtrRMtrR (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pET21a-NonGNonG (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pET21a-Orf2Orf2 (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pET21a-orfL6orfL6 (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pET21a-PaaRPaaR (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pET21a-PqrAPqrA (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pET21a-TylPTylP (Other)Bacterial Expression, Synthetic Biology Voigt Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
pET-Ce25Expresses a plant arginyl-tRNA synthetase in E.coliarginyl-tRNA synthetase (Other)Bacterial Expression Igloi Expression and properties of arginyl-tRNA synthetase from jack bean (Canavalia ensiformis). Protein Expr Purif. 2008 Oct;61(2):163-7. doi: 10.1016/j.pep.2008.05.008. Epub 2008 May 23.
pJ207 Delta-cpc-KanRDeletes the cpc operon in Synechocystis and confers kanamycin resistanceReplacing the cpc operon with kanamycin resistance (Synthetic)Synthetic Biology Melis Maximizing photosynthetic efficiency and culture productivity in cyanobacteria upon minimizing the phycobilisome light-harvesting antenna size. Biochim Biophys Acta. 2014 Oct;1837(10):1653-64. doi: 10.1016/j.bbabio.2014.07.009. Epub 2014 Jul 18.
psbA2-PHLS (b)Replaces the psbA2 gene in Synechocystis with the PHLS genebeta-phellandrene synthase (Other), Chloramphenicol resistanceBacterial Expression, Synthetic Biology Melis Regulation of β-phellandrene synthase gene expression, recombinant protein accumulation, and monoterpene hydrocarbons production in Synechocystis transformants. Planta May 2014
psbA2(noATbox)-PHLS (c)Replaces the psbA2 gene in Synechocystis with the PHLS genebeta-phellandrene synthase (Other), Chloramphenicol resistanceBacterial Expression, Synthetic Biology Melis Regulation of β-phellandrene synthase gene expression, recombinant protein accumulation, and monoterpene hydrocarbons production in Synechocystis transformants. Planta May 2014
psbA2-Ptrc-PHLS (d)Replaces the psbA2 gene in Synechocystis with the Ptrc promoter - PHLS genebeta-phellandrene synthase (Other), Chloramphenicol resistanceBacterial Expression, Synthetic Biology Melis Regulation of β-phellandrene synthase gene expression, recombinant protein accumulation, and monoterpene hydrocarbons production in Synechocystis transformants. Planta May 2014
psbA2-Ptrc-T7-g10-PHLS (e)Replaces the psbA2 gene in Synechocystis with the Ptrc promoter-T7-g10-PHLS gene beta-phellandrene synthase (Other), Chloramphenicol resistanceBacterial Expression, Synthetic Biology Melis Regulation of β-phellandrene synthase gene expression, recombinant protein accumulation, and monoterpene hydrocarbons production in Synechocystis transformants. Planta May 2014
cpc-PHLS (g)Replaces the cpc operon in Synechocystis with the PHLS genebeta-phellandrene synthase (Other), Chloramphenicol resistanceBacterial Expression, Synthetic Biology Melis Regulation of β-phellandrene synthase gene expression, recombinant protein accumulation, and monoterpene hydrocarbons production in Synechocystis transformants. Planta May 2014
pHL 100pLlac0-1:OxyS-mCherry, pLtetO-1:fhlA::gfp OxyS sRNA (Other), fhlA (Other)Bacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
pHL 762pLtetO-1:MicC, pLtetO-1:sodB::mCherry, pLlacO-1:ompC::gfp MicC sRNA (Other), sodB (Other), ompC (Other)Bacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
pHL 802pLtetO-1:OxyS-mCherry, pLlacO-1:ompC::gfp OxyS sRNA (Other), ompC (Other)Bacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
pHL 990pLtetO-1:DsrA, pCon:RhyB, pLlacO-1:rpoS::gfp DsrA sRNA (Other), RhyB sRNA (competitor) (Other), rpoS (Other)Bacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
pHL 1011pLlac0-1:RhyB-mCherry, pLtetO-1:RBS (st7) gfp RhyB sRNA (Other), RBS (st7) (Synthetic)Bacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
pHL 1019pLtetO-1:RhyB, pCon:MicC, pLlacO-1:sodB::gfp RhyB sRNA (Other), MicC sRNA (Competitor) (Other), sodB (Other)Bacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
pHL 1020pLtetO-1:RhyB, pCon:DsrA, pLlacO-1:sodB::gfp RhyB sRNA (Other), DsrA sRNA (competitor) (Other), sodB (Other)Bacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
pHL 1022pLtetO-1:RhyB, pCon:OxyS, pLlacO-1:sodB::gfp RhyB sRNA (Other), OxyS sRNA (Other), sodB (Other)Bacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
pHL 1032pLtetO-1:RhyB, pCon:ompC::mCherry, pLlacO-1:sodB::gfp RhyB sRNA (Other), ompC (Other), sodB (Other)Bacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
pHL 1033pLtetO-1:RhyB, pCon:rpoS::mCherry, pLlacO-1:sodB::gfp RhyB sRNA (Other), rpoS (Other), sodB (Other)Bacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
pHL 1035pLtetO-1:RhyB, pCon:fhlA::mCherry, pLlacO-1:sodB::gfp RhyB sRNA (Other), fhlA (Other), sodB (Other)Bacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
pHL 1039pLtetO-1:DsrA, pCon:OxyS, pLlacO-1:rpoS::gfp DsrA sRNA (Other), OxyS sRNA (competitor) (Other), rpoS (Other)Bacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
pHL 1057pCon:RhyB, pLlacO-1:rpoS::gfp RhyB sRNA (Other), rpoS (Other)Bacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
pHL 1070pCon:DsrA, pLlacO-1:sodB::gfp DsrA sRNA (Other), sodB (Other)Bacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
pHL 1073pCon:ompC::mCherry, pLlacO-1:sodB::gfp mCherry (Other), GFP (Other)Bacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
pHL 1075pCon:fhlA::mCherry, pLlacO-1:sodB::gfp fhlA (Other), sodB (Other)Bacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
pHL 1148pCon:OxyS, pLlacO-1:sodB::gfp OxyS sRNA (Other), sodB (Other)Bacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
pHL 1158pLtetO-1:RhyB, pCon:DsrA, pCon:ompC::mCherry, pLlacO-1:sodB::gfp RhyB sRNA (Other), DsrA sRNA (Other), ompC (Other), sodB (Other)Bacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
pHL 1303pLtetO-1:sodB::mCherry, pLlacO-1:ompC::gfp sodB (Other), ompC (Other)Bacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
pHL 1394pLtetO-1:RhyB, pCon:T7(st3) (133bps)::mCherry, pLlacO-1:sodB::gfp RhyB sRNA (Other), T7(st3) (133bps) (Other), sodB (Other)Bacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
pHL 1405pLtetO-1:RhyB, pCon:DsrA, pLtetO-1:hfq, pLlacO-1:sodB::gfp RhyB sRNA (Other), DsrA sRNA (Other), hfq (Other), sodB (Other)Bacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
pHL 1410pLtetO-1:DsrA, pCon:ompC::T7noRBS(900bps), pLlacO-1:rpoS::gfp DsrA sRNA (Other), ompC (Other), rpoS (Other)Bacterial Expression, Synthetic Biology Lim Disruption of small RNA signaling caused by competition for Hfq. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
pHL 342PLtetO-1:mCherry, PLlacO-1:araC, Plar:ompC::gfp mCherry (Synthetic), araC (Other), ompC (Other)Bacterial Expression, Synthetic Biology Lim Direct comparison of small RNA and transcription factor signaling. Nucleic Acids Res. 2012 May 22.
pHL 1023PLtetO-1:mCherry, PLlacO-1:gfp (st7) mCherry (Synthetic), gfp (st7) (Synthetic)Bacterial Expression, Synthetic Biology Lim Direct comparison of small RNA and transcription factor signaling. Nucleic Acids Res. 2012 May 22.
pHL 246AmpR + fimS (off orientation)::gfp::Asp terminator + CamR + ColE1 GFP (Other)Bacterial Expression, Synthetic Biology Lim Modulating the frequency and bias of stochastic switching to control phenotypic variation. Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574.
pHL 636 AmpR + PLtetO-1::RBS (st7) fimE::T1 terminator + PLlacO-1::RBS (st7) fimB::Asp terminator + ColE1 fimE (Other), fimB (Other)Bacterial Expression, Synthetic Biology Lim Lim Lab plasmids (unpublished)
pHL 651LacIq + PCon::RBS (st3) tetR::T1 terminator + p15a + CamR. Backbone from pZA32MCS1 (Lutz and Bujard, 1997). LacIq (Other), tetR (Other)Bacterial Expression, Synthetic Biology Lim Modulating the frequency and bias of stochastic switching to control phenotypic variation. Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574.
pHL 1277 AmpR + PLtetO-1::RBS (st7) gfp::T1 terminator + PLlacO-1::RBS (st7) mCherry::Asp terminator + ColE1 GFP (Other), mCherry (Synthetic)Bacterial Expression, Synthetic Biology Lim Modulating the frequency and bias of stochastic switching to control phenotypic variation. Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574.
pHL 1278AmpR + PLtetO-1::RBS (st7) mCherry::T1 terminator + PLlacO-1::RBS (st7) gfp::Asp terminator + ColE1 mCherry (Synthetic), GFP (Other)Bacterial Expression, Synthetic Biology Lim Modulating the frequency and bias of stochastic switching to control phenotypic variation. Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574.
pHL 1454AmpR + PLlacO-1::RBS (st7) fimB::Asp terminator + ColE1fimB (Other)Bacterial Expression, Synthetic Biology Lim Modulating the frequency and bias of stochastic switching to control phenotypic variation. Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574.
pHL 1903AmpR + fimS::gfpAAV::Asp terminator + CamR + ColE1GFP (Other)Bacterial Expression, Synthetic Biology Lim Modulating the frequency and bias of stochastic switching to control phenotypic variation. Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574.
pHL 1904AmpR + fimS::gfpLVA::Asp terminator + CamR + ColE1GFP (Other)Bacterial Expression, Synthetic Biology Lim Lim Lab plasmids (unpublished)
pHL 1915AmpR + PLlacO1fims::gfp::Asp terminator + CamR + ColE1 (PLlacO1fimS is a fused PLlacO-1 and fimS promoter)GFP (Other)Bacterial Expression, Synthetic Biology Lim Lim Lab plasmids (unpublished)
pHL 1916AmpR + PLtetO-1::RBS (st7) fimB::Asp terminator + ColE1fimB (Other)Bacterial Expression, Synthetic Biology Lim Modulating the frequency and bias of stochastic switching to control phenotypic variation. Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574.
pHL 1917AmpR + PCon:RBS (st7)::mCherry::T1 terminator + PLlacO-1::RBS (st7) fimB::Asp terminator + ColE1mCherry (Synthetic), fimB (Other)Bacterial Expression, Synthetic Biology Lim Modulating the frequency and bias of stochastic switching to control phenotypic variation. Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574.
pHL 1977AmpR + PLtetO-1::RBS (st3) fimB::Asp terminator + ColE1fimB (Other)Bacterial Expression, Synthetic Biology Lim Lim Lab plasmids (unpublished)
pHL1506PLlacO-1:RBS(st7):csrA csrA (Other)Bacterial Expression, Synthetic Biology Lim Rapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation. Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22.
pHL1529PLlacO-1:glgC::gfp, PconNoHind:RBS(st3):tetR, PconNoHind:RBS(st2):csrA GFP (Other), tetR, csrA (Other)Bacterial Expression, Synthetic Biology Lim Rapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation. Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22.
pHL1575PconNoHind:csrB, PLtetO-1:RBS(st3):csrD csrB (Other), csrD (Other)Bacterial Expression, Synthetic Biology Lim Rapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation. Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22.
pHL1590PLtetO-1:RBS(st3):csrAcsrA (Other)Bacterial Expression, Synthetic Biology Lim Lim Lab plasmids (unpublished)
pHL1722PLtetO-1:glgClead::mCherry, PLlacO-1:RBS(st7):csrA mCherry (Other), csrA (Other)Bacterial Expression, Synthetic Biology Lim Lim Lab plasmids (unpublished)
pHL1724PLtetO-1:pgaAleadFull::mCherry, PLlacO-1:RBS(st7):csrA mCherry (Other), csrA (Other)Bacterial Expression, Synthetic Biology Lim Lim Lab plasmids (unpublished)
pHL1726PLtetO-1:hfqlead::mCherry, PLlacO-1:RBS(st7):csrA mCherry (Other), csrA (Other)Bacterial Expression, Synthetic Biology Lim Lim Lab plasmids (unpublished)
pHL1745PLtetO-1:cstAlead::mCherry, PLlacO-1:RBS(st7):csrA mCherry (Other), csrA (Other)Bacterial Expression, Synthetic Biology Lim Lim Lab plasmids (unpublished)
pHL1746PLtetO-1:ycdTlead::mCherry, PLlacO-1:RBS(st7):csrA mCherry (Other), csrA (Other)Bacterial Expression, Synthetic Biology Lim Lim Lab plasmids (unpublished)
pHL1747PLtetO-1:ydeHlead::mCherry, PLlacO-1:RBS(st7):csrA mCherry (Other), csrA (Other)Bacterial Expression, Synthetic Biology Lim Lim Lab plasmids (unpublished)
pHL1756PconNoHindM12:glgC::gfp, PconNoHind:RBS(st3):tetR GFP (Other), tetR (Other)Bacterial Expression, Synthetic Biology Lim Rapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation. Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22.
pHL1757PconNoHindM2:glgC::gfp, PconNoHind:RBS(st3):tetR GFP (Other), tetR (Other)Bacterial Expression, Synthetic Biology Lim Rapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation. Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22.
pHL1790PLtetO-1:glgClead::mCherry, PLlacO-1:csrB mCherry (Other), csrB (Other)Bacterial Expression, Synthetic Biology Lim Lim Lab plasmids (unpublished)
pHL1801PLlacO-1:RBS(st7):gfp GFP (Other)Bacterial Expression, Synthetic Biology Lim Rapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation. Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22.
pHL1853PLlacO-1:glgC::gfp::LVA, PconNoHind:RBS(st3):tetR, PconNoHind:RBS(st2):csrA GFP (Other), tetR (Other), csrA (Other)Bacterial Expression, Synthetic Biology Lim Rapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation. Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22.
pHL1855PLlacO-1:glgC::gfp::LVA, PconNoHind:RBS(st3):tetR, PconNoHind:RBS(st3):csrA GFP (Other), tetR (Other), csrA (Other)Bacterial Expression, Synthetic Biology Lim Rapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation. Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22.
pHL1914PconNoHindM2:hfqlead::gfp, PconNoHind:RBS(st3):tetR GFP (Other), tetR (Other)Bacterial Expression, Synthetic Biology Lim Lim Lab plasmids (unpublished)
pHL1928PLtetO-1:Agn43RBS:mCherry, PLlacO-1:RBS(st7):csrA mCherry (Other), csrA (Other)Bacterial Expression, Synthetic Biology Lim Lim Lab plasmids (unpublished)
pHL1940PLtetO-1:cstA‡, PLlacO-1:RBS(st7):csrA cstA (Other), csrA (Other)Bacterial Expression, Synthetic Biology Lim Lim Lab plasmids (unpublished)
pHL1947PconNoHindM12:glgC::gfp, PconNoHind:RBS(st3):tetR, PconNoHindM8:RBS(st3):csrA GFP (Other), tetR (Other), csrA (Other)Bacterial Expression, Synthetic Biology Lim Lim Lab plasmids (unpublished)
pHL1948PconNoHindM12:glgC::gfp, PconNoHind:RBS(st3):tetR, PconNoHindM10:RBS(st3):csrA GFP (Other), tetR (Other), csrA (Other)Bacterial Expression, Synthetic Biology Lim Lim Lab plasmids (unpublished)
pP3-GFPControls GFP expression from the P3 promoter cloned out of S. aureus RN4220 genomeP3 promoter (Other)Bacterial Expression, Synthetic Biology Collins Peptide-based communication system enables Escherichia coli to Bacillus megaterium interspecies signaling. Biotechnol Bioeng. 2013 Nov;110(11):3003-12. doi: 10.1002/bit.24975. Epub 2013 Jul 5.
pXylA-agrCA-Ixylose-dependent expression of the type-I agrC and agrA genes of S. aureusagrCA locus (Other)Bacterial Expression, Synthetic Biology Collins Peptide-based communication system enables Escherichia coli to Bacillus megaterium interspecies signaling. Biotechnol Bioeng. 2013 Nov;110(11):3003-12. doi: 10.1002/bit.24975. Epub 2013 Jul 5.
pXylA-agrCA-IVxylose-dependent expression of the type-IV agrC and type-I agrA genes of S. aureusagrCA locus (Other)Bacterial Expression, Synthetic Biology Collins Peptide-based communication system enables Escherichia coli to Bacillus megaterium interspecies signaling. Biotechnol Bioeng. 2013 Nov;110(11):3003-12. doi: 10.1002/bit.24975. Epub 2013 Jul 5.
pT7-agrBD-IT7-dependent expression of the type-I agrB and agrD genes of S. aureus RN4220agrBD locus (Other)Bacterial Expression, Synthetic Biology Collins Peptide-based communication system enables Escherichia coli to Bacillus megaterium interspecies signaling. Biotechnol Bioeng. 2013 Nov;110(11):3003-12. doi: 10.1002/bit.24975. Epub 2013 Jul 5.
pT7-agrBD-IVDerivative of pT7-agrBD-I with a point mutation in the agrD gene to change AIP coding region from type-I to type-IVagrBD locus (with D29Y mutation in agrD) (Other)Bacterial Expression, Synthetic Biology Collins Peptide-based communication system enables Escherichia coli to Bacillus megaterium interspecies signaling. Biotechnol Bioeng. 2013 Nov;110(11):3003-12. doi: 10.1002/bit.24975. Epub 2013 Jul 5.
pET-Gm1Expresses a plant arginyl-tRNA synthetase in E.coliarginyl-tRNA synthetase (Other)Bacterial Expression Igloi Amino acid discrimination by arginyl-tRNA synthetases as revealed by an examination of natural specificity variants. FEBS J. 2009 Mar;276(5):1307-18. doi: 10.1111/j.1742-4658.2009.06866.x.
pET-Cb2Expresses an insect mitochondrial arginyl-tRNA synthetase in E.colimitochondrial arginyl-tRNA synthetase (Other)Bacterial Expression Igloi Amino acid discrimination by the nuclear encoded mitochondrial arginyl-tRNA synthetase of the larva of a bruchid beetle (Caryedes brasiliensis) from northwestern Costa Rica. Insect Biochem Mol Biol. 2013 Dec;43(12):1172-80.
pBS1CEmpty vector, integration at amyE, ampr, cmrSynthetic Biology ; Bacillus BioBrick Box Mascher The Bacillus BioBrick Box: generation and evaluation of essential genetic building blocks for standardized work with Bacillus subtilis. J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29.
pBS2EEmpty vector, integration at lacA, ampr, mlsrSynthetic Biology ; Bacillus BioBrick Box Mascher The Bacillus BioBrick Box: generation and evaluation of essential genetic building blocks for standardized work with Bacillus subtilis. J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29.
pBS4SEmpty vector, integration at thrC, ampr, specrSynthetic Biology ; Bacillus BioBrick Box Mascher The Bacillus BioBrick Box: generation and evaluation of essential genetic building blocks for standardized work with Bacillus subtilis. J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29.
pBS1ClacZlacZ-reporter vector, integration at amyE, ampr, cmrSynthetic Biology ; Bacillus BioBrick Box Mascher The Bacillus BioBrick Box: generation and evaluation of essential genetic building blocks for standardized work with Bacillus subtilis. J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29.
pBS3Cluxlux-reporter vector, integration at sacA, ampr, cmrSynthetic Biology ; Bacillus BioBrick Box Mascher The Bacillus BioBrick Box: generation and evaluation of essential genetic building blocks for standardized work with Bacillus subtilis. J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29.
PvegVery strong constitutive promoterveg promoter (Other)Synthetic Biology ; Bacillus BioBrick Box Mascher The Bacillus BioBrick Box: generation and evaluation of essential genetic building blocks for standardized work with Bacillus subtilis. J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29.
PliaGConstitutive promoterLiaG promoter (Other)Synthetic Biology ; Bacillus BioBrick Box Mascher The Bacillus BioBrick Box: generation and evaluation of essential genetic building blocks for standardized work with Bacillus subtilis. J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29.
PlepAStrong constitutive promoterlepA promoter (Other)Synthetic Biology ; Bacillus BioBrick Box Mascher The Bacillus BioBrick Box: generation and evaluation of essential genetic building blocks for standardized work with Bacillus subtilis. J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29.
J23101Very weak constitutive promoterJ23101 promoter (Other)Synthetic Biology ; Bacillus BioBrick Box Mascher The Bacillus BioBrick Box: generation and evaluation of essential genetic building blocks for standardized work with Bacillus subtilis. J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29.
PliaIBacitracin-inducible promoterliaI promoter (Other)Synthetic Biology ; Bacillus BioBrick Box Mascher The Bacillus BioBrick Box: generation and evaluation of essential genetic building blocks for standardized work with Bacillus subtilis. J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29.
PxylAXylose-inducible promoterxylA promoter (Other)Synthetic Biology ; Bacillus BioBrick Box Mascher The Bacillus BioBrick Box: generation and evaluation of essential genetic building blocks for standardized work with Bacillus subtilis. J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29.
His10-tag10xHis-tagHis10 (Synthetic)Synthetic Biology ; Bacillus BioBrick Box Mascher The Bacillus BioBrick Box: generation and evaluation of essential genetic building blocks for standardized work with Bacillus subtilis. J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29.
FLAG-tagFLAG-tagFLAG (Synthetic)Synthetic Biology ; Bacillus BioBrick Box Mascher The Bacillus BioBrick Box: generation and evaluation of essential genetic building blocks for standardized work with Bacillus subtilis. J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29.
StrepII-tagStreptactin-tagStrepII tag (Synthetic)Synthetic Biology ; Bacillus BioBrick Box Mascher The Bacillus BioBrick Box: generation and evaluation of essential genetic building blocks for standardized work with Bacillus subtilis. J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29.
HA-tagHA-tagHA tag (Synthetic)Synthetic Biology ; Bacillus BioBrick Box Mascher The Bacillus BioBrick Box: generation and evaluation of essential genetic building blocks for standardized work with Bacillus subtilis. J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29.
cMyc-tagcMyc tagcMyc tag (Synthetic)Synthetic Biology ; Bacillus BioBrick Box Mascher The Bacillus BioBrick Box: generation and evaluation of essential genetic building blocks for standardized work with Bacillus subtilis. J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29.
pCS1Produces aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.KanR (Other)Bacterial Expression Bennett Stable maintenance of multiple plasmids in E. coli using a single selective marker. ACS Synth Biol. 2012 Oct 19;1(10):445-50. doi: 10.1021/sb3000589. Epub 2012 Sep 4.
pCS5Produces one half (beta) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.KanR (Other)Bacterial Expression Bennett Stable maintenance of multiple plasmids in E. coli using a single selective marker. ACS Synth Biol. 2012 Oct 19;1(10):445-50. doi: 10.1021/sb3000589. Epub 2012 Sep 4.
pCS4Produces one half (alpha) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.KanR (Other)Bacterial Expression Bennett Stable maintenance of multiple plasmids in E. coli using a single selective marker. ACS Synth Biol. 2012 Oct 19;1(10):445-50. doi: 10.1021/sb3000589. Epub 2012 Sep 4.
pCS6Produces T7 RNA Polymerase.T7 RNA Polymerase (Other)Bacterial Expression Bennett Stable maintenance of multiple plasmids in E. coli using a single selective marker. ACS Synth Biol. 2012 Oct 19;1(10):445-50. doi: 10.1021/sb3000589. Epub 2012 Sep 4.
pSEVAT1Z1AGeneGuard vector cassette: thyA, R6K COR, Zeta toxin; with ampR as cargo.Bacterial Expression, Synthetic Biology Ellis GeneGuard: a Modular Plasmid System Designed for Biosafety. ACS Synth Biol. 2014 May 13.
pSEVAD1Z1AGeneGuard vector cassette: dapA, R6K COR, Zeta toxin; with ampR as cargo.Bacterial Expression, Synthetic Biology Ellis GeneGuard: a Modular Plasmid System Designed for Biosafety. ACS Synth Biol. 2014 May 13.
pSEVAT1K1AGeneGuard vector cassette: thyA, R6K COR, Kid toxin; with ampR as cargo.Bacterial Expression, Synthetic Biology Ellis GeneGuard: a Modular Plasmid System Designed for Biosafety. ACS Synth Biol. 2014 May 13.
pSEVAD1K1AGeneGuard vector cassette: dapA, R6K COR, Kid toxin; with ampR as cargo.Bacterial Expression, Synthetic Biology Ellis GeneGuard: a Modular Plasmid System Designed for Biosafety. ACS Synth Biol. 2014 May 13.
pSEVAT7Z1AGeneGuard vector cassette: thyA, ColE2 COR, Zeta toxin; with ampR as cargo.Bacterial Expression, Synthetic Biology Ellis GeneGuard: a Modular Plasmid System Designed for Biosafety. ACS Synth Biol. 2014 May 13.
pSEVAD7Z1AGeneGuard vector cassette: dapA, ColE2 COR, Zeta toxin; with ampR as cargo.Bacterial Expression, Synthetic Biology Ellis GeneGuard: a Modular Plasmid System Designed for Biosafety. ACS Synth Biol. 2014 May 13.
pSEVAT7K1AGeneGuard vector cassette: thyA, ColE2 COR, Kid toxin; with ampR as cargo.Bacterial Expression, Synthetic Biology Ellis GeneGuard: a Modular Plasmid System Designed for Biosafety. ACS Synth Biol. 2014 May 13.
pSEVAD7K1AGeneGuard vector cassette: dapA, ColE2 COR, Kid toxin; with ampR as cargo.Bacterial Expression, Synthetic Biology Ellis GeneGuard: a Modular Plasmid System Designed for Biosafety. ACS Synth Biol. 2014 May 13.
pSEVA12DKR-AGeneGuard genomic cassette: for constructing ΔdapA::RBS A pi, Kis, FRT-kanR-FRT (low-copy support for R6K COR). Will support pSEVAD1K.Genomic cassette (Synthetic)Bacterial Expression, Synthetic Biology Ellis GeneGuard: a Modular Plasmid System Designed for Biosafety. ACS Synth Biol. 2014 May 13.
pSEVA12DKR-EGeneGuard genomic cassette: for constructing ΔdapA::RBS E pi, Kis, FRT-kanR-FRT (medium-copy support for R6K COR). Will support pSEVAD1K.Genomic cassette (Synthetic)Bacterial Expression, Synthetic Biology Ellis GeneGuard: a Modular Plasmid System Designed for Biosafety. ACS Synth Biol. 2014 May 13.
pSEVA12DKR-JGeneGuard genomic cassette: for constructing ΔdapA::RBS J pi, Kis, FRT-kanR-FRT (high-copy support for R6K COR). Will support pSEVAD1K.Genomic cassette (Synthetic)Bacterial Expression, Synthetic Biology Ellis GeneGuard: a Modular Plasmid System Designed for Biosafety. ACS Synth Biol. 2014 May 13.
pSEVA12TEC-BGeneGuard genomic cassette: for constructing ΔthyA::RBS B repA, Epsilon, FRT-kanR-FRT (low-copy support for ColE2 COR). Will support pSEVAT7Z.Genomic cassette (Synthetic)Bacterial Expression, Synthetic Biology Ellis GeneGuard: a Modular Plasmid System Designed for Biosafety. ACS Synth Biol. 2014 May 13.
pSEVA12TEC-IGeneGuard genomic cassette: for constructing ΔthyA::RBS I repA, Epsilon, FRT-kanR-FRT (medium-copy support for ColE2 COR). Will support pSEVAT7Z.Genomic cassette (Synthetic)Bacterial Expression, Synthetic Biology Ellis GeneGuard: a Modular Plasmid System Designed for Biosafety. ACS Synth Biol. 2014 May 13.
pSEVA3b61Shuttle vector: pUC ori, pTHT15 ori for use in E. coli/B. subtilis. Cannot modify orientation of cat gene; promoter too weak in E. coli, probably transcribed via repB promoter in bicistronic manner.Bacterial Expression, Synthetic Biology Ellis GeneGuard: a Modular Plasmid System Designed for Biosafety. ACS Synth Biol. 2014 May 13.
pSEVA3b67RbShuttle vector: contains constitutive mRFP1, compatible as reporter for E. coli/B. subtilis. See pSEVA3b61 for more info.mRFP1 (Synthetic)Bacterial Expression, Synthetic Biology Ellis GeneGuard: a Modular Plasmid System Designed for Biosafety. ACS Synth Biol. 2014 May 13.
p005kanRFPRFP from coral Corynactis sp. under T5 promoter with lac operators; kanamycin resistant; to be used in practicalsyntheticbiology.net/resourcesRed fluorescent protein (Other)Bacterial Expression, Synthetic Biology Bryk Practical synthetic biology (unpublished)
pTE100MethyloBrick cloning vector, no promoter; TcRBacterial Expression, Synthetic Biology Erb A Set of Versatile Brick Vectors and Promoters for the Assembly, Expression, and Integration of Synthetic Operons in Methylobacterium extorquens AM1 and Other Alphaproteobacteria. ACS Synth Biol. 2014 Aug 8.
pTE101MethyloBrick cloning vector, no promoter; KmRBacterial Expression, Synthetic Biology Erb A Set of Versatile Brick Vectors and Promoters for the Assembly, Expression, and Integration of Synthetic Operons in Methylobacterium extorquens AM1 and Other Alphaproteobacteria. ACS Synth Biol. 2014 Aug 8.
pTE102MethyloBrick expression vector, mxaF promoter; TcRBacterial Expression, Synthetic Biology Erb A Set of Versatile Brick Vectors and Promoters for the Assembly, Expression, and Integration of Synthetic Operons in Methylobacterium extorquens AM1 and Other Alphaproteobacteria. ACS Synth Biol. 2014 Aug 8.
pTE103MethyloBrick expression vector, fumC promoter; TcRBacterial Expression, Synthetic Biology Erb A Set of Versatile Brick Vectors and Promoters for the Assembly, Expression, and Integration of Synthetic Operons in Methylobacterium extorquens AM1 and Other Alphaproteobacteria. ACS Synth Biol. 2014 Aug 8.
pTE104MethyloBrick expression vector, coxB promoter; TcRBacterial Expression, Synthetic Biology Erb A Set of Versatile Brick Vectors and Promoters for the Assembly, Expression, and Integration of Synthetic Operons in Methylobacterium extorquens AM1 and Other Alphaproteobacteria. ACS Synth Biol. 2014 Aug 8.
pTE105MethyloBrick expression vector, tuf promoter; TcRBacterial Expression, Synthetic Biology Erb A Set of Versatile Brick Vectors and Promoters for the Assembly, Expression, and Integration of Synthetic Operons in Methylobacterium extorquens AM1 and Other Alphaproteobacteria. ACS Synth Biol. 2014 Aug 8.
pBbE1k-2dihydropterine reductase from human, pterin-4a-carbinolamine dehydratase from humandihydropterine reductase, pterin-4a-carbinolamine dehydratase Lee Engineering of L-tyrosine oxidation in Escherichia coli and microbial production of hydroxytyrosol. Metab Eng. 2012 Nov;14(6):603-10. doi: 10.1016/j.ymben.2012.08.002. Epub 2012 Aug 29.
pTHSSd_1Splitposon in KanR ColE1 backboneSplitposon w/ KanR, LacI & PTac (Synthetic)Bacterial Expression Voigt A 'resource allocator' for transcription based on a highly fragmented T7 RNA polymerase. Mol Syst Biol. 2014 Jul 30;10(7):742.
pTHSSd_8PT7 GFP reportersfGFP (Synthetic)Bacterial Expression Voigt A 'resource allocator' for transcription based on a highly fragmented T7 RNA polymerase. Mol Syst Biol. 2014 Jul 30;10(7):742.
pTHSSd_9PT3 GFP reportersfGFP (Synthetic)Bacterial Expression Voigt A 'resource allocator' for transcription based on a highly fragmented T7 RNA polymerase. Mol Syst Biol. 2014 Jul 30;10(7):742.
pTHSSd_10PK1F GFP reportersfGFP (Synthetic)Bacterial Expression Voigt A 'resource allocator' for transcription based on a highly fragmented T7 RNA polymerase. Mol Syst Biol. 2014 Jul 30;10(7):742.
pTHSSd_11PCGG GFP reportersfGFP (Synthetic)Bacterial Expression Voigt A 'resource allocator' for transcription based on a highly fragmented T7 RNA polymerase. Mol Syst Biol. 2014 Jul 30;10(7):742.
pTHSSd_14Triple split (at 67, 601-SZ)Three-fragment T7* RNA polymerase (Synthetic)Bacterial Expression Voigt A 'resource allocator' for transcription based on a highly fragmented T7 RNA polymerase. Mol Syst Biol. 2014 Jul 30;10(7):742.
pTHSSd_18Quad split (at 61, 179, 601-SZ)Four-fragment T7* RNA polymerase (Synthetic)Bacterial Expression Voigt A 'resource allocator' for transcription based on a highly fragmented T7 RNA polymerase. Mol Syst Biol. 2014 Jul 30;10(7):742.
pTHSSd_23PTac T7 sigma fragment expressionT7 sigma fragment (Synthetic)Bacterial Expression Voigt A 'resource allocator' for transcription based on a highly fragmented T7 RNA polymerase. Mol Syst Biol. 2014 Jul 30;10(7):742.
pTHSSd_24PTac T3 sigma fragment expressionT3 sigma fragment (Synthetic)Bacterial Expression Voigt A 'resource allocator' for transcription based on a highly fragmented T7 RNA polymerase. Mol Syst Biol. 2014 Jul 30;10(7):742.
pTHSSd_25PTac K1FR sigma fragment expressionK1FR sigma fragment (Synthetic)Bacterial Expression Voigt A 'resource allocator' for transcription based on a highly fragmented T7 RNA polymerase. Mol Syst Biol. 2014 Jul 30;10(7):742.
pTHSSd_26PTac CGG sigma fragment expressionCGG sigma fragment (Synthetic)Bacterial Expression Voigt A 'resource allocator' for transcription based on a highly fragmented T7 RNA polymerase. Mol Syst Biol. 2014 Jul 30;10(7):742.
pTHSSd_27PTac null fragment (sigma CGG Y639A) expressionnull fragment (Synthetic)Bacterial Expression Voigt A 'resource allocator' for transcription based on a highly fragmented T7 RNA polymerase. Mol Syst Biol. 2014 Jul 30;10(7):742.
pTHSSd_29PTac alpha fragment expressionalpha fragment (Synthetic)Bacterial Expression Voigt A 'resource allocator' for transcription based on a highly fragmented T7 RNA polymerase. Mol Syst Biol. 2014 Jul 30;10(7):742.
pTHSSd_34PTac GFP expressionsfGFP (Synthetic)Bacterial Expression Voigt A 'resource allocator' for transcription based on a highly fragmented T7 RNA polymerase. Mol Syst Biol. 2014 Jul 30;10(7):742.
pTHSSd_38High core fragment expression (high resource allocator)core fragment (Synthetic)Bacterial Expression Voigt A 'resource allocator' for transcription based on a highly fragmented T7 RNA polymerase. Mol Syst Biol. 2014 Jul 30;10(7):742.
pTHSSd_39Low core fragment expression (low resource allocator)core fragment (Synthetic)Bacterial Expression Voigt A 'resource allocator' for transcription based on a highly fragmented T7 RNA polymerase. Mol Syst Biol. 2014 Jul 30;10(7):742.
pTHSSd_40High core fragment expression without SynZIPcore fragment lacking SynZIP (Synthetic)Bacterial Expression Voigt A 'resource allocator' for transcription based on a highly fragmented T7 RNA polymerase. Mol Syst Biol. 2014 Jul 30;10(7):742.
pTHSSd_41High full length T7 RNAP* expressionT7* RNAP (Synthetic)Bacterial Expression Voigt A 'resource allocator' for transcription based on a highly fragmented T7 RNA polymerase. Mol Syst Biol. 2014 Jul 30;10(7):742.
pTHSSd_42beta core fragment expressionbeta core fragment (Synthetic)Bacterial Expression Voigt A 'resource allocator' for transcription based on a highly fragmented T7 RNA polymerase. Mol Syst Biol. 2014 Jul 30;10(7):742.
pTHSSd_44constitutive expression of T7 sigma fragmentT7 sigma fragment (Synthetic)Bacterial Expression Voigt A 'resource allocator' for transcription based on a highly fragmented T7 RNA polymerase. Mol Syst Biol. 2014 Jul 30;10(7):742.
pBAM1This plasmids is a modular mini-Tn5 vector that could be used to generate random mutagenesis libraries or to insert heterologous DNA into a target genome Synthetic Biology de Lorenzo pBAM1: an all-synthetic genetic tool for analysis and construction of complex bacterial phenotypes. BMC Microbiol. 2011 Feb 22;11:38. doi: 10.1186/1471-2180-11-38.
pReporter_2A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).Reporter_2 (Synthetic)Bacterial Expression Voigt Permanent genetic memory with >1-byte capacity. Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
pReporter_3A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).Reporter_3 (Synthetic)Bacterial Expression Voigt Permanent genetic memory with >1-byte capacity. Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
pReporter_4A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).Reporter_4 (Synthetic)Bacterial Expression Voigt Permanent genetic memory with >1-byte capacity. Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
pReporter_5A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).Reporter_5 (Synthetic)Bacterial Expression Voigt Permanent genetic memory with >1-byte capacity. Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
pReporter_7A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).Reporter_7 (Synthetic)Bacterial Expression Voigt Permanent genetic memory with >1-byte capacity. Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
pReporter_8A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).Reporter_8 (Synthetic)Bacterial Expression Voigt Permanent genetic memory with >1-byte capacity. Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
pReporter_9A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).Reporter_9 (Synthetic)Bacterial Expression Voigt Permanent genetic memory with >1-byte capacity. Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
pReporter_10A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).Reporter_10 (Synthetic)Bacterial Expression Voigt Permanent genetic memory with >1-byte capacity. Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
pReporter_11A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).Reporter_11 (Synthetic)Bacterial Expression Voigt Permanent genetic memory with >1-byte capacity. Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
pReporter_12A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).Reporter_12 (Synthetic)Bacterial Expression Voigt Permanent genetic memory with >1-byte capacity. Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
pIntegrase_2plasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadIntegrase_2 (Other)Bacterial Expression Voigt Permanent genetic memory with >1-byte capacity. Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
pIntegrase_3plasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadIntegrase_3 (Synthetic)Bacterial Expression Voigt Permanent genetic memory with >1-byte capacity. Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
pIntegrase_4plasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadIntegrase_4 (Other)Bacterial Expression Voigt Permanent genetic memory with >1-byte capacity. Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
pIntegrase_5plasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadIntegrase_5 (Other)Bacterial Expression Voigt Permanent genetic memory with >1-byte capacity. Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
pIntegrase_7plasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadIntegrase_7 (Other)Bacterial Expression Voigt Permanent genetic memory with >1-byte capacity. Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
pIntegrase_8plasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadIntegrase_8 (Other)Bacterial Expression Voigt Permanent genetic memory with >1-byte capacity. Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
pIntegrase_9plasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadIntegrase_9 (Other)Bacterial Expression Voigt Permanent genetic memory with >1-byte capacity. Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
pIntegrase_10plasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadIntegrase_10 (Other)Bacterial Expression Voigt Permanent genetic memory with >1-byte capacity. Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
pIntegrase_11plasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadIntegrase_11 (Other)Bacterial Expression Voigt Permanent genetic memory with >1-byte capacity. Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
pIntegrase_12plasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadIntegrase_12 (Other)Bacterial Expression Voigt Permanent genetic memory with >1-byte capacity. Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
pIntegrase_13plasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadIntegrase_13 (Other)Bacterial Expression Voigt Permanent genetic memory with >1-byte capacity. Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
pMemoryArrayAn array constructed by concatenating the attB/P sites of the 11 phage integrases and can be used to distinguish 2048 possible combinations of events.MemoryArray (Synthetic)Bacterial Expression Voigt Permanent genetic memory with >1-byte capacity. Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
pCis_2+5a 2-integrase plasmidCis_2+5 (Synthetic)Bacterial Expression Voigt Permanent genetic memory with >1-byte capacity. Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
pCis_7+8a 2-integrase plasmidCis_7+8 (Synthetic)Bacterial Expression Voigt Permanent genetic memory with >1-byte capacity. Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
pCis_7+8+10a 3-integrase plasmidCis_7+8+10 (Synthetic)Bacterial Expression Voigt Permanent genetic memory with >1-byte capacity. Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
pCis_2+7+8+5a 4-integrase plasmidCis_2+7+8+5 (Synthetic)Bacterial Expression Voigt Permanent genetic memory with >1-byte capacity. Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
pAND_Int2A transcriptional AND circuit constructed by layering NOR and NOT gatesAND_Int2 (Synthetic)Bacterial Expression Voigt Permanent genetic memory with >1-byte capacity. Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
pAND_ReporterAND gate reporterAND_Reporter (Synthetic)Bacterial Expression Voigt Permanent genetic memory with >1-byte capacity. Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
pAND_yfpused with pSpec to generate an AND gate without memory AND_yfp (Synthetic)Bacterial Expression Voigt Permanent genetic memory with >1-byte capacity. Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
pSpecused with pAND_YFP to generate an AND gate without memory Spec (Synthetic)Bacterial Expression Voigt Permanent genetic memory with >1-byte capacity. Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
pCas_2+5encodes the Int2 gene controlled by PBad promoter and a reversed Int5 gene flanked by the attB/P sites of Int2Cas_2+5 (Synthetic)Bacterial Expression Voigt Permanent genetic memory with >1-byte capacity. Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
pCas_5+7_gfpencodes a reversed Int7 gene flanked by the attB/P sites of Int5, as well as a reversed GFP (GFPmut33) gene flanked by the attB/P sites of Int7. Cas_5+7_gfp (Synthetic)Bacterial Expression Voigt Permanent genetic memory with >1-byte capacity. Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
pTara:500Expresses T7 RNAP from the arabinose inducible promoter PBAD with a RBS strength of 500 from the Salis RBS calculator. Also includes a constitutive araC ORFT7 RNAP (Other)Bacterial Expression, Synthetic Biology Bennett Library of synthetic transcriptional AND gates built with split T7 RNA polymerase mutants. Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 10.1073/pnas.1220157110. Epub 2013 Mar 11.
pTSaraExpresses both fragments of T7 RNAP split at position 179. Both fragments are driven by the arabinose inducible promoter PBAD. Also contains a constitutive araC ORFN Terminal fragment of T7 RNAP split betwen 179-180 (Synthetic), N Terminal fragment of T7 RNAP split betwen 179-180 (Other)Bacterial Expression Bennett Library of synthetic transcriptional AND gates built with split T7 RNA polymerase mutants. Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 10.1073/pnas.1220157110. Epub 2013 Mar 11.
pTSara-NExpresses the N-terminal fragment of split T7 RNAP split a position 179. Insert is driven by PBAD. Contains a constitutive araC ORFResidues 1-179 of T7 RNAP (Synthetic)Bacterial Expression, Synthetic Biology Bennett Library of synthetic transcriptional AND gates built with split T7 RNA polymerase mutants. Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 10.1073/pnas.1220157110. Epub 2013 Mar 11.
pTSara-CExpresses the C-terminal fragment of split T7 RNAP split a position 179. Insert is driven by the arabinose inducible promoter PBAD. Contains a constitutive araC ORFResidues 180-880 of T7 RNAP (Synthetic)Bacterial Expression, Synthetic Biology Bennett Library of synthetic transcriptional AND gates built with split T7 RNA polymerase mutants. Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 10.1073/pnas.1220157110. Epub 2013 Mar 11.
pTSara-Q758CExpresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation Q758C. Contains a constitutive araC ORFResidues 1-179 of split T7 RNAP (Synthetic), Residues 180-880 of split T7 RNAP (Synthetic)Bacterial Expression, Synthetic Biology Bennett Library of synthetic transcriptional AND gates built with split T7 RNA polymerase mutants. Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 10.1073/pnas.1220157110. Epub 2013 Mar 11.
pTSara-R756SExpresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation R756S. Contains a constitutive araC ORFResidues 1-179 of split T7 RNAP (Synthetic), Residues 180-880 of split T7 RNAP (Synthetic)Bacterial Expression, Synthetic Biology Bennett Library of synthetic transcriptional AND gates built with split T7 RNA polymerase mutants. Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 10.1073/pnas.1220157110. Epub 2013 Mar 11.
pTSara-N748DExpresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation N748D. Contains a constitutive araC ORFResidues 1-179 of split T7 RNAP (Synthetic), Residues 180-880 of split T7 RNAP (Synthetic)Bacterial Expression, Synthetic Biology Bennett Library of synthetic transcriptional AND gates built with split T7 RNA polymerase mutants. Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 10.1073/pnas.1220157110. Epub 2013 Mar 11.
pTSara-R756KExpresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation R756K. Contains a constitutive araC ORFResidues 1-179 of split T7 RNAP (Synthetic), Residues 180-880 of split T7 RNAP (Synthetic)Bacterial Expression, Synthetic Biology Bennett Library of synthetic transcriptional AND gates built with split T7 RNA polymerase mutants. Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 10.1073/pnas.1220157110. Epub 2013 Mar 11.
pTSlb-wtExpresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. Residues 1-179 of split T7 RNAP (Synthetic), Residues 180-880 of split T7 RNAP (Synthetic)Bacterial Expression, Synthetic Biology Bennett Library of synthetic transcriptional AND gates built with split T7 RNA polymerase mutants. Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 10.1073/pnas.1220157110. Epub 2013 Mar 11.
pTSlb-Q758CExpresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations Q758CResidues 1-179 of split T7 RNAP (Synthetic), Residues 180-880 of split T7 RNAP (Synthetic)Bacterial Expression, Synthetic Biology Bennett Library of synthetic transcriptional AND gates built with split T7 RNA polymerase mutants. Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 10.1073/pnas.1220157110. Epub 2013 Mar 11.
pTSlb-R756SExpresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations R756SResidues 1-179 of split T7 RNAP (Synthetic), Residues 180-880 of split T7 RNAP (Synthetic)Bacterial Expression, Synthetic Biology Bennett Library of synthetic transcriptional AND gates built with split T7 RNA polymerase mutants. Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 10.1073/pnas.1220157110. Epub 2013 Mar 11.
pTSlb-R756KExpresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations R756SResidues 1-179 of split T7 RNAP (Synthetic), Residues 180-880 of split T7 RNAP (Synthetic)Bacterial Expression, Synthetic Biology Bennett Library of synthetic transcriptional AND gates built with split T7 RNA polymerase mutants. Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 10.1073/pnas.1220157110. Epub 2013 Mar 11.
pTSlb-N748DExpresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations N748D.Residues 1-179 of split T7 RNAP (Synthetic), Residues 180-880 of split T7 RNAP (Synthetic)Bacterial Expression, Synthetic Biology Bennett Library of synthetic transcriptional AND gates built with split T7 RNA polymerase mutants. Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 10.1073/pnas.1220157110. Epub 2013 Mar 11.
pET28:GFPpET28 driving expression of GFP, biobrick part Bba_E0040GFPBacterial Expression, Synthetic Biology Bennett Library of synthetic transcriptional AND gates built with split T7 RNA polymerase mutants. Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 10.1073/pnas.1220157110. Epub 2013 Mar 11.
pET:GACT:GFPA modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GACT' between -11 and -8.GFPBacterial Expression, Synthetic Biology Bennett Library of synthetic transcriptional AND gates built with split T7 RNA polymerase mutants. Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 10.1073/pnas.1220157110. Epub 2013 Mar 11.
pET:GACG:GFPA modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GACG' between -11 and -8.GFPBacterial Expression, Synthetic Biology Bennett Library of synthetic transcriptional AND gates built with split T7 RNA polymerase mutants. Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 10.1073/pnas.1220157110. Epub 2013 Mar 11.
pET:GCAT:GFPA modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GCAT' between -11 and -8.GFPBacterial Expression, Synthetic Biology Bennett Library of synthetic transcriptional AND gates built with split T7 RNA polymerase mutants. Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 10.1073/pnas.1220157110. Epub 2013 Mar 11.
pET:CACT:GFPA modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'CACT' between -11 and -8.GFPBacterial Expression, Synthetic Biology Bennett Library of synthetic transcriptional AND gates built with split T7 RNA polymerase mutants. Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 10.1073/pnas.1220157110. Epub 2013 Mar 11.
pET:CCCT:GFPA modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'CCCT' between -11 and -8.GFPBacterial Expression, Synthetic Biology Bennett Library of synthetic transcriptional AND gates built with split T7 RNA polymerase mutants. Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 10.1073/pnas.1220157110. Epub 2013 Mar 11.
pET:ACAT:GFPA modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'ACAT' between -11 and -8.GFPBacterial Expression, Synthetic Biology Bennett Library of synthetic transcriptional AND gates built with split T7 RNA polymerase mutants. Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 10.1073/pnas.1220157110. Epub 2013 Mar 11.
pZS1[RbsR-L]Contains PIq driving expression RbsR-L, the Ribose inducible chimera with the wt Lac DBD. RbsR-L (Synthetic)Bacterial Expression, Synthetic Biology Bennett Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras. ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28.
pZS1[FruR-L]Contains PIq driving expression of the FruR-L, the Fructose inducible chimera with the wt Lac DBD.FruR-L (Synthetic)Bacterial Expression, Synthetic Biology Bennett Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras. ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28.
pzs1[GalS-L]Contains PIq driving expression of GalS-R, the Fucose inducible chimera with the wt Lac DBD. GalS-L (Synthetic)Bacterial Expression, Synthetic Biology Bennett Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras. ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28.
pZS1[LacI-L]Contains PI driving expression dimeric LacI, the Lactose inducible repressor. LacI (Other)Bacterial Expression, Synthetic Biology Bennett Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras. ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28.
pZS1[RbsR-T]Contains PIq driving expression RbsR-T, the Ribose inducible chimera with the TAN DBD.RbsR-T (Other)Bacterial Expression, Synthetic Biology Bennett Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras. ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28.
pZS1[FruR-T]Contains PIq driving expression FruR-T, the Fructose inducible chimera with the TAN DBD.FruR-T (Other), FruR-T (Other)Bacterial Expression, Synthetic Biology Bennett Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras. ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28.
pZS1[GalS-T]Contains PIq driving expression GalS-T, the Fucose inducible chimera with the TAN DBD.GalS-T (Other), GalS-T (Synthetic)Bacterial Expression, Synthetic Biology Bennett Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras. ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28.
pZS1[TreR-T]Contains PI driving expression TreR-T, the Trehalose inducible chimera with the TAN DBD.TreR-T (Other), GalS-T (Synthetic)Bacterial Expression, Synthetic Biology Bennett Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras. ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28.
pZS1[Iq:LacI-T]Contains PIq driving expression LacI-T, the Lactose inducible chimera with the TAN DBD.LacI-T (Other)Bacterial Expression, Synthetic Biology Bennett Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras. ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28.
pZS1[RbsR-L,LacI-L]Contains PIq driving expression RbsR-L, and PI driving expression of LacI-L.RbsR-L (Synthetic), Dimeric LacI (Synthetic)Bacterial Expression, Synthetic Biology Bennett Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras. ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28.
pZS1[GalS-L,LacI-L]Contains PIq driving expression GalS-L, and PI driving expression of LacI-L.GalS-L (Synthetic), wt LacI (Synthetic)Bacterial Expression, Synthetic Biology Bennett Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras. ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28.
pZS1[TreR-L,LacI-L]Contains PI driving expression TreR-L, and PI driving expression of LacI-LTreR-L (Synthetic), Dimeric LacI (Synthetic)Bacterial Expression, Synthetic Biology Bennett Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras. ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28.
pZS1[FruR-L,RbsR-L]Contains PIq driving expression TreR-L, and PIq driving expression of RbsR-LFruR-L (Synthetic), RbsR-L (Synthetic)Bacterial Expression, Synthetic Biology Bennett Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras. ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28.
pZS1[GalS-L,RbsR-L]Contains PIq driving expression GalS-L, and PIq driving expression of RbsR-L.GalS-L (Synthetic), RbsR-L (Synthetic)Bacterial Expression, Synthetic Biology Bennett Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras. ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28.
pZS1[GalS-L,FruR-L]Contains PIq driving expression GalS-L, and PIq driving expression of FruR-L.GalS-L (Synthetic), FruR-L (Synthetic)Bacterial Expression, Synthetic Biology Bennett Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras. ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28.
pZS1[GalS-L,TreR-L]Contains PIq driving expression GalS-L, and PI driving expression of TreR-L.GalS-L (Synthetic), TreR-L (Synthetic)Bacterial Expression, Synthetic Biology Bennett Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras. ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28.
pZS1[RbsR-L,TreR-L]Contains PIq driving expression RbsR-L, and PI driving expression of TreR-L.RbsR-L (Synthetic), TreR-L (Synthetic)Bacterial Expression, Synthetic Biology Bennett Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras. ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28.
pZS1[RbsR-T,LacI-T]Contains PIq driving expression RbsR-T, and PI driving expression of TreR-L.RbsR-L (Synthetic), Dimeric LacI with the TAN DBD (Synthetic)Bacterial Expression, Synthetic Biology Bennett Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras. ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28.
pZS1[FruR-T,LacI-T]Contains PIq driving expression FruR-T, and PI driving expression of LacI-T.FruR-T (Synthetic), Dimeric LacI with the TAN DBD (Synthetic)Bacterial Expression, Synthetic Biology Bennett Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras. ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28.
pZS1[TreR-T,LacI-T]Contains PI driving expression TreR-T, and PI driving expression of LacI-T.TreR-T (Synthetic), Dimeric LacI with the TAN DBD (Synthetic)Bacterial Expression, Synthetic Biology Bennett Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras. ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28.
pZS1[FruR-T,TreR-T]Contains PIq driving expression FruR-T, and PIq driving expression of TreR-T.FruR-T (Synthetic), TreR-T (Synthetic)Bacterial Expression, Synthetic Biology Bennett Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras. ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28.
pZS1[GalS-T,RbsR-T]Contains PIq driving expression GalS-T, and PIq driving expression of RbsR-T.GalS-T (Synthetic), GalS-T (Synthetic)Bacterial Expression, Synthetic Biology Bennett Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras. ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28.
pZS1[GalS-T,TreR-T]Contains PIq driving expression GalS-T, and PI driving expression of TreR-T.GalS-T (Synthetic), TreR-T (Synthetic)Bacterial Expression, Synthetic Biology Bennett Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras. ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28.
pZS1[RbsR-T,TreR-T]Contains PIq driving expression RbsR-T, and PI driving expression of TreR-T.RbsR-L (Synthetic), TreR-T (Synthetic)Bacterial Expression, Synthetic Biology Bennett Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras. ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28.
pZM3[I:FruR-L,I:RbsR-L]Contains PI driving expression FruR-L, and PI driving expression of RbsR-L.FruR-L (Synthetic), RbsR-L (Synthetic) Bennett Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras. ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28.
pAE03To construct C-terminal GFP fusions, single crossover integration vectorBacterial Expression, Synthetic Biology Veening Cellular localization of choline-utilization proteins in Streptococcus pneumoniae using novel fluorescent reporter systems. Mol Microbiol. 2009 Oct;74(2):395-408. doi: 10.1111/j.1365-2958.2009.06872.x. Epub 2009 Sep 8.
pJWV25To construct N-terminal GFP fusions under control of a Zn2+ inducible promoter, integrates via double crossover at bgaABacterial Expression, Synthetic Biology Veening Cellular localization of choline-utilization proteins in Streptococcus pneumoniae using novel fluorescent reporter systems. Mol Microbiol. 2009 Oct;74(2):395-408. doi: 10.1111/j.1365-2958.2009.06872.x. Epub 2009 Sep 8.
pPEP1BglFusion compatible integration vector for Streptococcus pneumoniaeBacterial Expression, Synthetic Biology Veening Gene Expression Platform for Synthetic Biology in the Human Pathogen Streptococcus pneumoniae. ACS Synth Biol. 2014 Jun 4.
pCM433kanTSucrose counterselection (sacB) vector for unmarked allelic exchange.Bacterial Expression, Synthetic Biology ; Sucrose counterselection Lidstrom Genetic tools for the industrially promising methanotroph Methylomicrobium buryatense. Appl Environ Microbiol. 2014 Dec 29. pii: AEM.03795-14.
pAWP78Small IncP-based broad host range replicating vector.Bacterial Expression, Synthetic Biology Lidstrom Genetic tools for the industrially promising methanotroph Methylomicrobium buryatense. Appl Environ Microbiol. 2014 Dec 29. pii: AEM.03795-14.
pAWP89IncP-based replicating vector expressing dTomato under tac promoter.Ptac-dTomatoBacterial Expression, Synthetic Biology Lidstrom Genetic tools for the industrially promising methanotroph Methylomicrobium buryatense. Appl Environ Microbiol. 2014 Dec 29. pii: AEM.03795-14.
pcrRNA.indArabinose-inducible repeat-only E. coli CRISPR arrayBacterial Expression, CRISPR, Synthetic Biology Beisel Repurposing endogenous type I CRISPR-Cas systems for programmable gene repression. Nucleic Acids Res. 2014 Oct 17. pii: gku971.
pBW313lux-hrpRpBWF2620 encoding rbs33-hrpR-ter (pBWF2620: pSB3K3 carrying BBa_F2620 (PTet-rbs34-luxR-ter))F2620-rbs33-hrpR-ter (Synthetic)Bacterial Expression Buck Engineering modular and orthogonal genetic logic gates for robust digital-like synthetic biology. Nat Commun. 2011 Oct 18;2:508. doi: 10.1038/ncomms1516.
pBW400hrpL-gfppBW400hrpL-gfphrpL-rbs30-gfp-ter (Synthetic)Bacterial Expression Buck Engineering modular and orthogonal genetic logic gates for robust digital-like synthetic biology. Nat Commun. 2011 Oct 18;2:508. doi: 10.1038/ncomms1516.
pBW412hrpL-cIgfppSB4A3 harbouring hrpL-rbs32-cI-lva-ter-Plam-rbs30-gfp-terHrpL-rbs32-CI-LVA-t-plam-30gfp-t (Synthetic)Bacterial Expression Buck Engineering modular and orthogonal genetic logic gates for robust digital-like synthetic biology. Nat Commun. 2011 Oct 18;2:508. doi: 10.1038/ncomms1516.
pBW414hrpL-cIgfppSB4A3 harbouring hrpL-rbs34-cI-lva-ter-Plam-rbs30-gfp-terHrpL-rbs34-CI-LVA-t-plam-30gfp-t (Synthetic)Bacterial Expression Buck Engineering modular and orthogonal genetic logic gates for robust digital-like synthetic biology. Nat Commun. 2011 Oct 18;2:508. doi: 10.1038/ncomms1516.
pFF145aTc-inducible Beta recombinaseBeta recombinase (Other)Synthetic Biology Lu Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations. Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
pFF530IPTG-inducible msd_kanR(ON)msd_kanR(ON) (Other)Synthetic Biology Lu Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations. Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
pFF706Light-inducible SCRIBE_kanR(ON)SCRIBE_kanR(ON) (Other)Synthetic Biology Lu Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations. Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
pFF714IPTG-inducible SCRIBE_galK(OFF)SCRIBE_galK(OFF) (Other)Synthetic Biology Lu Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations. Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
pFF745IPTG-inducible SCRIBE_kanR(ON)SCRIBE_kanR(ON) (Other)Synthetic Biology Lu Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations. Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
pFF746aTc-inducible SCRIBE_galK(ON)SCRIBE_galK(ON) (Other)Synthetic Biology Lu Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations. Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
pFF749IPTG-inducible msd_kanR(ON)_dRTmsd_kanR(ON)_dRT (Other)Synthetic Biology Lu Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations. Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
pFF753IPTG-inducible msd(wt)msd(wt) (Other)Synthetic Biology Lu Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations. Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
pFF758IPTG-inducible msd(wt)_dRTmsd(wt)_dRT (Other)Synthetic Biology Lu Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations. Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
pFF761aTc-inducible SCRIBE_galK(OFF)SCRIBE_galK(OFF) (Other)Synthetic Biology Lu Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations. Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
pFF828AHL-inducible msd_lacZ(ON)msd_lacZ(ON) (Other)Synthetic Biology Lu Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations. Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
pFF838aTc-inducible SCRIBE_lacZ(ON)SCRIBE_lacZ(ON) (Other)Synthetic Biology Lu Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations. Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
pBAMD1-2Standardized mini-Tn5 delivery plasmid, kanamycin resistantSynthetic Biology ; Mini-Tn5 vector de Lorenzo New transposon tools tailored for metabolic engineering of gram-negative microbial cell factories. Front Bioeng Biotechnol. 2014 Oct 28;2:46. doi: 10.3389/fbioe.2014.00046. eCollection 2014.
pBAMD1-4Standardized mini-Tn5 delivery plasmid, streptomycin/spectinomycin resistantSynthetic Biology ; Mini-Tn5 vector de Lorenzo New transposon tools tailored for metabolic engineering of gram-negative microbial cell factories. Front Bioeng Biotechnol. 2014 Oct 28;2:46. doi: 10.3389/fbioe.2014.00046. eCollection 2014.
pBAMD1-6Standardized mini-Tn5 delivery plasmid, gentamicin resistantSynthetic Biology ; Mini-Tn5 vector de Lorenzo New transposon tools tailored for metabolic engineering of gram-negative microbial cell factories. Front Bioeng Biotechnol. 2014 Oct 28;2:46. doi: 10.3389/fbioe.2014.00046. eCollection 2014.
pMU102Cloning and expression vector for Clostridium thermocellumBacterial Expression, Synthetic Biology Lynd Computational design and characterization of a temperature-sensitive plasmid replicon for gram positive thermophiles. J Biol Eng. 2012 May 11;6(1):5. doi: 10.1186/1754-1611-6-5.
pAN-PTet-dCas9controls the expression of S. pyogenes dCas9 from a Tc‐inducible PTet promoter.dCas9 (Other)Bacterial Expression, CRISPR Voigt Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks. Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
pAN-PTet-YFPused to characterize the promoter activities of PTet for dCas9 and sgRNA response functions.YFP (Other)Bacterial Expression Voigt Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks. Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
pAN-PBAD-YFPused to characterize the promoter activities of PBAD for dCas9 and sgRNA response functions.YFP (Other)Bacterial Expression Voigt Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks. Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
pAN-PA1-RFPreporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1mRFP (Other)Bacterial Expression Voigt Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks. Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
pAN-PA2-RFPreporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1mRFP (Other)Bacterial Expression Voigt Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks. Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
pAN-PA3-RFPreporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1mRFP (Other)Bacterial Expression Voigt Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks. Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
pAN-PA4-RFPreporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1mRFP (Other)Bacterial Expression Voigt Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks. Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
pAN-PA5-RFPreporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1mRFP (Other)Bacterial Expression Voigt Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks. Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
pAN-PBAD-sgRNA-A1NTexpression of A1NT sgRNA from the arabinose-inducible promoterA1NT (Synthetic)Bacterial Expression, CRISPR Voigt Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks. Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
pAN-PBAD-sgRNA-A1Texpression of A1T sgRNA from the arabinose-inducible promoterA1T sgRNA (Synthetic)Bacterial Expression, CRISPR Voigt Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks. Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
pAN-PBAD-sgRNA-A2NTexpression of A2NT sgRNA from the arabinose-inducible promoterA2NT sgRNABacterial Expression, CRISPR Voigt Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks. Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
pAN-PBAD-sgRNA-A2Texpression of A2T sgRNA from the arabinose-inducible promoterA2T sgRNA (Synthetic)Bacterial Expression, CRISPR Voigt Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks. Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
pAN-PBAD-sgRNA-A3NTexpression of A3NT sgRNA from the arabinose-inducible promoterA3NT sgRNA (Synthetic)Bacterial Expression, CRISPR Voigt Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks. Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
pAN-PBAD-sgRNA-A3Texpression of A3T sgRNA from the arabinose-inducible promoterA3T sgRNA (Synthetic)Bacterial Expression, CRISPR Voigt Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks. Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
pAN-PBAD-sgRNA-A4NTexpression of A4NT sgRNA from the arabinose-inducible promoterA4NT sgRNA (Synthetic)Bacterial Expression, CRISPR Voigt Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks. Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
pAN-PBAD-sgRNA-A4Texpression of A4T sgRNA from the arabinose-inducible promoterA4T sgRNA (Synthetic)Bacterial Expression, CRISPR Voigt Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks. Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
pAN-PBAD-sgRNA-A5NTexpression of A5NT sgRNA from the arabinose-inducible promoterA5NT sgRNA (Synthetic)Bacterial Expression, CRISPR Voigt Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks. Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
pAN-PBAD-sgRNA-A5Texpression of A5T sgRNA from the arabinose-inducible promoterA5T sgRNA (Synthetic)Bacterial Expression, CRISPR Voigt Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks. Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
pAN-PBAD-sgRNA-scrambleexpression of scramble sgRNA from the arabinose-inducible promoterscramble sgRNA (Synthetic)Bacterial Expression, CRISPR Voigt Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks. Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
pAN-PBAD-sgRNA-VRexpression of VR sgRNA from the arabinose-inducible promoterVR sgRNA (Synthetic)Bacterial Expression, CRISPR Voigt Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks. Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
pAN-NORsynthetic circuit: NOR gateA2NT sgRNA (Synthetic), A2NT sgRNA (Synthetic)Bacterial Expression, CRISPR Voigt Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks. Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
pAN-ORsynthetic circuit: OR gateA2NT sgRNA (Synthetic), A4NT sgRNA (Synthetic), A2NT sgRNA (Synthetic)Bacterial Expression, CRISPR Voigt Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks. Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
pAN-ANDsynthetic circuit: AND gateA1NT sgRNA (Synthetic), A4NT sgRNA (Synthetic), A2NT sgRNA (Synthetic), A1NT (Synthetic)Bacterial Expression, CRISPR Voigt Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks. Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
pAN-OR-MalT-3NTsynthetic circuit: OR gate, the sgRNA used for the NOT gate was substituted with one designed to target the malT gene in the E. coli genomeA2NT sgRNA (Synthetic), Malt-3NT sgRNA (Synthetic), A2NT sgRNA (Synthetic)Bacterial Expression, CRISPR Voigt Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks. Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
pJKR-H-cdaRGlucarate biosensor. High copy plasmid that expresses GFP proportional to glucarate concentration.beta lactamase, sfGFP, CdaRBacterial Expression Church Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
pJKR-L-cdaRGlucarate biosensor. Low copy plasmid that expresses GFP proportional to glucarate concentration.GFPBacterial Expression Church Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
pJKR-H-mphRMacrolide biosensor. High copy plasmid that expresses GFP proportional to macrolide concentration.GFPBacterial Expression Church Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
pJKR-L-mphRMacrolide biosensor. Low copy plasmid that expresses GFP proportional to macrolide concentration.GFPBacterial Expression Church Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
pJKR-H-tetRTetracycline biosensor. High copy plasmid that expresses GFP proportional to tetracycline concentration.GFPBacterial Expression Church Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
pJKR-L-tetRTetracycline biosensor. Low copy plasmid that expresses GFP proportional to tetracycline concentration.GFPBacterial Expression Church Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
pJKR-H-araCArabinose biosensor. High copy plasmid that expresses GFP proportional to arabinose concentration.GFPBacterial Expression Church Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
pJKR-H-ttgRFlavonoid biosensor. High copy plasmid that expresses GFP proportional to flavonoid concentration.GFPBacterial Expression Church Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
pJKR-L-ttgRFlavonoid biosensor. Low copy plasmid that expresses GFP proportional to flavonoid concentration.GFPBacterial Expression Church Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
pJKR-H-acuRAcrylate biosensor. High copy plasmid that expresses GFP proportional to acrylate concentration.GFPBacterial Expression Church Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
pJKR-L-acuRAcrylate biosensor. Low copy plasmid that expresses GFP proportional to acrylate concentration.GFPBacterial Expression Church Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
pJKR-O-acuRAcrylate biosensor. Medium copy plasmid that expresses CFP proportional to acrylate concentration. Compatible with all other pJKR plasmids.CFPBacterial Expression Church Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
pJKR-O-mphRMacrolide biosensor. Medium copy plasmid that expresses GFP proportional to macrolide concentration. Compatible with all other pJKR plasmids.mCherryBacterial Expression Church Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
p148ePathBrick pETM6 vector encoding CsF3H, AaDFR, and DuLARflavaone 3B-hydroxylase (Other), dihydroflavonol 4-reductase (Other), leucoanthocyanidin reductase (Other)Bacterial Expression, Synthetic Biology Koffas Improvement of catechin production in Escherichia coli through combinatorial metabolic engineering. Metab Eng. 2014 Dec 17;28C:43-53. doi: 10.1016/j.ymben.2014.12.002.
p158ePathBrick pETM6 vector encoding CsF3H, CsDFR, and DuLARflavaone 3B-hydroxylase (Other), dihydroflavonol 4-reductase (Other), leucoanthocyanidin reductase (Other)Bacterial Expression, Synthetic Biology Koffas Improvement of catechin production in Escherichia coli through combinatorial metabolic engineering. Metab Eng. 2014 Dec 17;28C:43-53. doi: 10.1016/j.ymben.2014.12.002.
p168ePathBrick pETM6 vector encoding CsF3H, FaDFR, and DuLARflavaone 3B-hydroxylase (Other), dihydroflavonol 4-reductase (Other), leucoanthocyanidin reductase (Other)Bacterial Expression, Synthetic Biology Koffas Improvement of catechin production in Escherichia coli through combinatorial metabolic engineering. Metab Eng. 2014 Dec 17;28C:43-53. doi: 10.1016/j.ymben.2014.12.002.
p168-759ePathBrick pETM6 vector encoding CsF3H, AaDFR, DuLAR with Protein Scaffold GBD:SH3:PDZ (1:1:4)flavaone 3B-hydroxylase (Other), dihydroflavonol 4-reductase (Other), leucoanthocyanidin reductase (Other), Scaffold Protein encoding GBD:SH3:PDZ (1:1:4) (Synthetic)Bacterial Expression, Synthetic Biology Koffas Improvement of catechin production in Escherichia coli through combinatorial metabolic engineering. Metab Eng. 2014 Dec 17;28C:43-53. doi: 10.1016/j.ymben.2014.12.002.
p1585588ePathBrick pETM6 vector encoding CsF3H, CsDFR, and DuLAR (1:3:3 Ratio)flavaone 3B-hydroxylase (Other), dihydroflavonol 4-reductase (Other), leucoanthocyanidin reductase (Other), dihydroflavonol 4-reductase (Other), dihydroflavonol 4-reductase (Other), leucoanthocyanidin reductase (Other), leucoanthocyanidin reductase (Other)Bacterial Expression, Synthetic Biology Koffas Improvement of catechin production in Escherichia coli through combinatorial metabolic engineering. Metab Eng. 2014 Dec 17;28C:43-53. doi: 10.1016/j.ymben.2014.12.002.
pJexpress414_OmpA171_R4consConservative version of OmpA Redesign 4OmpA_R4cons (Synthetic)Bacterial Expression, Synthetic Biology Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
pJexpress414_OmpA171_WTWild-type OmpA (N-terminal domain) with an HA tag in the 4th external loopOmpA (Other)Bacterial Expression, Synthetic Biology Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
pJexpress414_OmpA171_H1346OmpA redesign hybrid with wild-type strands 1346OmpA_H1346 (Synthetic)Bacterial Expression, Synthetic Biology Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
pJexpress414_OmpA171_H1256OmpA redesign hybrid with wild-type strands 1256OmpA_H1256 (Synthetic)Bacterial Expression, Synthetic Biology Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
pJexpress414_OmpA171_H2345OmpA redesign hybrid with wild-type strands 2345OmpA_H2345 (Synthetic)Bacterial Expression, Synthetic Biology Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
pJexpress414_OmpA171_H3567OmpA redesign hybrid with wild-type strands 3567OmpA_H3567 (Synthetic)Bacterial Expression, Synthetic Biology Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
pJexpress414_OmpA171_H2368OmpA redesign hybrid with wild-type strands 2368OmpA_H2368 (Synthetic)Bacterial Expression, Synthetic Biology Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
pJexpress414_OmpA171_H1678OmpA redesign hybrid with wild-type strands 1678OmpA_H1678 (Synthetic)Bacterial Expression, Synthetic Biology Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
pJexpress414_OmpA171_WTnoGirdlesWild-type OmpA with aromatic girdle residues replaced by leucineOmpA_noGirdles (Synthetic)Bacterial Expression, Synthetic Biology Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
pHL391Lim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + PLtetO-1::RBS (st7) hfq::mCherry::T1 terminator + ColE1gfp, mCherryBacterial Expression, Synthetic Biology Lim Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
pHL417Lim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + PLtetO-1::micC::Asp terminator + pLtetO-1::RBS (st7) hfq::T1 terminator + ColE1gfp, micC leader, hfq leaderBacterial Expression, Synthetic Biology Lim Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
pHL518Lim Lab plasmid: AmpR + PLlacO-1::micC::mCherry::T1 terminator + PLtetO-1m9::OmpC::gfp::T1T2 terminator + ColE1mCherry, gfpBacterial Expression, Synthetic Biology Lim Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
pHL671Lim Lab plasmid: AmpR + PCon::glgC::gfp::T1T2 terminator + ColE1gfpBacterial Expression, Synthetic Biology Lim Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
pHL718Lim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + PLtetO-1::ipex::mCherry::T1 terminator + ColE1gfp, mCherryBacterial Expression, Synthetic Biology Lim Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
pHL836Lim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + PLtetO-1::ipex::Asp terminator + PLtetO-1::RBS (st7) hfq::T1 terminator + ColE1gfp, ipex leader, hfq leaderBacterial Expression, Synthetic Biology Lim Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
pHL1017Lim Lab plasmid: AmpR + PConNoHindM8::glgC::gfp::T1T2 terminator + ColE1gfpBacterial Expression, Synthetic Biology Lim Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
pHL1632Lim Lab plasmid: AmpR + PLlacO-1::RBS(st7) gfp::Asp terminator + ColE1gfpBacterial Expression, Synthetic Biology Lim Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
pHL1633Lim Lab plasmid: AmpR + PLlacO-1::T710 gfp::Asp terminator + ColE1gfpBacterial Expression, Synthetic Biology Lim Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
pHL1954Lim Lab plasmid: AmpR + PconNoHindM8::ryhB::Asp terminator + PLlacO-1::RBS (st7) hfq::T1 terminator + PLtetO-1m9::sodB::gfp::T1T2 terminator + ColE1M8::ryhB leader, hfq leader, gfpBacterial Expression, Synthetic Biology Lim Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
pHL1969Lim Lab plasmid: AmpR + PconNoHindM8::ryhB::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::sodB::gfp::T1T2 terminator + ColE1M8::ryhB leader, T710 hfq leader, gfpBacterial Expression, Synthetic Biology Lim Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
pHL2021Lim Lab plasmid: AmpR + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::sodB::gfp::T1T2 terminator + ColE1T710 hfq leader, gfpBacterial Expression, Synthetic Biology Lim Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
pHL2026Lim Lab plasmid: AmpR + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::glmS::gfp::T1T2 terminator + ColE1T710 hfq leader, gfpBacterial Expression, Synthetic Biology Lim Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
pHL2027Lim Lab plasmid: AmpR + PLlacO-1::T710 hfq::T1terminator + PLtetO-1::glmS::gfp::T1T2 terminator + ColE1T710 hfq leader, gfpBacterial Expression, Synthetic Biology Lim Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
pHL2053Lim Lab plasmid: AmpR + PLlacO-1::T710::hfq::T1 termintor + PLtetO-1m9::ompC::gfp::T1T2 terminator + ColE1T710 hfq leader, gfpBacterial Expression, Synthetic Biology Lim Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
pHL2073Lim Lab plasmid: AmpR + PconShortNoHind::glmZ::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::glmS::gfp::T1T2 terminator + ColE1glmZ leader, T710 hfq leader, gfpBacterial Expression, Synthetic Biology Lim Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
pHL2075Lim Lab plasmid: AmpR + PconShortNoHind::glmZ::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1::glmS::gfp::T1T2 terminator + ColE1glmZ leader, T710 hfq leader, gfpBacterial Expression, Synthetic Biology Lim Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
pHL2084Lim Lab plasmid: AmpR + PconShortNoHindM8::glmZ::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::glmS::gfp::T1T2 terminator + ColE1m8::glmZ leader, T710 hfq leader, gfpBacterial Expression, Synthetic Biology Lim Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
pHL2085Lim Lab plasmid: AmpR + PconShortNoHindM8::glmZ::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1::glmS::gfp::T1T2 terminator + ColE1m8::glmZ leader, T710 hfq leader, gfpBacterial Expression, Synthetic Biology Lim Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
pHL2107Lim Lab plasmid: AmpR + PconNoHindM8::micC::Asp terminator + PLlacO-1::T710::hfq::T1 terminator + PLtetO-1m9::ompC::gfp::T1T2 terminator + ColE1m8::micC leader, T710 hfq leader, gfpBacterial Expression, Synthetic Biology Lim Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
pHL2117Lim Lab plasmid: AmpR + PconNoHindM8::ryhB::Asp terminator + PLtetO-1m9::sodB::gfp::T1T2 terminator + ColE1M8::ryhB leader, gfpBacterial Expression, Synthetic Biology Lim Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding. Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
pReporter_13_switchBPA reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp). attP upstream of attB in this plasmid.Reporter_13_switchBP (Synthetic)Bacterial Expression Voigt Permanent genetic memory with >1-byte capacity. Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
pSR59.4Expresses OmpR constitutively from PompB97 and sfGFP under the PompF146 promoterOmpR (Other), sfGFP (Synthetic)Bacterial Expression, Synthetic Biology Tabor Refactoring and optimization of light-switchable escherichia coli two-component systems. ACS Synth Biol. 2014 Nov 21;3(11):820-31. doi: 10.1021/sb500273n. Epub 2014 Oct 11.
pSR58.6Expresses CcaR constitutively and sfGFP under the PcpcG2-172 promoterCcaR (Other), sfGFP (Synthetic)Bacterial Expression, Synthetic Biology Tabor Refactoring and optimization of light-switchable escherichia coli two-component systems. ACS Synth Biol. 2014 Nov 21;3(11):820-31. doi: 10.1021/sb500273n. Epub 2014 Oct 11.
pSR43.6rExpresses CcaS, Ho1, and PcyA constitutivelyCcaS (Other), Ho1 (Other), PcyA (Other)Bacterial Expression, Synthetic Biology Tabor Refactoring and optimization of light-switchable escherichia coli two-component systems. ACS Synth Biol. 2014 Nov 21;3(11):820-31. doi: 10.1021/sb500273n. Epub 2014 Oct 11.
pSR33.4rExpresses Cph8, Ho1, and PcyA constitutivelyCph8 (Synthetic), Ho1 (Other), PcyA (Other)Bacterial Expression, Synthetic Biology Tabor Refactoring and optimization of light-switchable escherichia coli two-component systems. ACS Synth Biol. 2014 Nov 21;3(11):820-31. doi: 10.1021/sb500273n. Epub 2014 Oct 11.
pUAP1Accepts DNA sequences via BpiI cloning sites, resulting in Level 0 standard parts for Golden Gate cloning. Parts can be released with a user-defined 4-base-pair, 5 prime overhangs using BsaI.RFP cloning selection cassette (Synthetic)Synthetic Biology Patron Standards for plant synthetic biology: a common syntax for exchange of DNA parts. New Phytol. 2015 Jul 14. doi: 10.1111/nph.13532.
AEB-No-aptamer-controlmRFP1This pFTV1 vector contains a no-aptamer control mRNA encoding mRFP1 gene. It should be used when testing AEB-F12 and AEB-Theo45 riboswitches to measure their non-specific ligand effect on mRFP1 expression.No-aptamer control mRNA (Synthetic)Synthetic Biology Salis Automated physics-based design of synthetic riboswitches from diverse RNA aptamers. Nucleic Acids Res. 2015 Nov 30. pii: gkv1289.
AEB-F12This pFTV1 vector contains a fluoride binding riboswitch, controlling the translation initaition rate of mRFP1 gene. F12 fluoride riboswitch (Synthetic)Synthetic Biology Salis Automated physics-based design of synthetic riboswitches from diverse RNA aptamers. Nucleic Acids Res. 2015 Nov 30. pii: gkv1289.
AEB-Theo45This pFTV1 vector contains a theophylline binding riboswitch, controlling the translation initaition rate of mRFP1 gene. Theo45 theophylline riboswitch (Synthetic)Synthetic Biology Salis Automated physics-based design of synthetic riboswitches from diverse RNA aptamers. Nucleic Acids Res. 2015 Nov 30. pii: gkv1289.
AEB-No-aptamer-control -- AEB3mRFP1This pFTV1 vector contains a no-aptamer control mRNA encoding mRFP1 gene, using a weak AEB3 promoter. It should be used when testing AEB-DNT3 riboswitch to measure the non-specific DNT effect on mRFP1 expression.No-aptamer control mRNA (Synthetic)Synthetic Biology Salis Automated physics-based design of synthetic riboswitches from diverse RNA aptamers. Nucleic Acids Res. 2015 Nov 30. pii: gkv1289.
AEB-DNT3This pFTV1 vector containsa 2,4,dinitrotoluene (DNT) binding riboswitch, controlling the translation initaition rate of mRFP1 gene. DNT3 DNT riboswitch (Synthetic)Synthetic Biology Salis Automated physics-based design of synthetic riboswitches from diverse RNA aptamers. Nucleic Acids Res. 2015 Nov 30. pii: gkv1289.
AEB-No-aptamer-controlluciferaseThis pFTV1 vector contains a no-aptamer control mRNA encoding luciferase gene. It should be used when testing AEB-Dopa5, AEB-T4-2 riboswitches to measure their non-specific ligand effect on luciferase expression.No-aptamer control mRNA (Synthetic)Synthetic Biology Salis Automated physics-based design of synthetic riboswitches from diverse RNA aptamers. Nucleic Acids Res. 2015 Nov 30. pii: gkv1289.
AEB-Dopa5This pFTV1 vector contains a dopamine binding riboswitch, controlling the translation initaition rate of luciferase gene. Dopa5 dopamine riboswitch (Synthetic)Synthetic Biology Salis Automated physics-based design of synthetic riboswitches from diverse RNA aptamers. Nucleic Acids Res. 2015 Nov 30. pii: gkv1289.
AEB-T4-2This pFTV1 vector contains a thyroxine binding riboswitch, controlling the translation initaition rate of luciferase gene. T4-2 thyroxine riboswitch (Synthetic)Synthetic Biology Salis Automated physics-based design of synthetic riboswitches from diverse RNA aptamers. Nucleic Acids Res. 2015 Nov 30. pii: gkv1289.
Mishler2014-No-aptamer-controlluciferase-pUC19This pUC19 vector contains a no-aptamer control mRNA encoding luciferase gene. It should be used when testing AEB-TMR7 and AEB-TMR10 riboswitches (all on pUC19) to measure their non-specific ligand effect on luciferase expression.No-aptamer control mRNA (Synthetic)Synthetic Biology Salis Automated physics-based design of synthetic riboswitches from diverse RNA aptamers. Nucleic Acids Res. 2015 Nov 30. pii: gkv1289.
AEB-TMR7pUC19This pUC19 vector contains a tetramethylrosamine (TMR) binding riboswitch, controlling the translation initaition rate of luciferase gene. TMR7 TMR riboswitch (Synthetic)Synthetic Biology Salis Automated physics-based design of synthetic riboswitches from diverse RNA aptamers. Nucleic Acids Res. 2015 Nov 30. pii: gkv1289.
AEB-TMR10pUC19This pUC19 vector contains a tetramethylrosamine (TMR) binding riboswitch, controlling the translation initaition rate of luciferase gene. TMR10 TMR riboswitch (Synthetic)Synthetic Biology Salis Automated physics-based design of synthetic riboswitches from diverse RNA aptamers. Nucleic Acids Res. 2015 Nov 30. pii: gkv1289.
pEERM1 KmPlasmid for integration into psbA2 site of Synechocystis 6803Bacterial Expression, Synthetic Biology Lindberg Metabolic Engineering of Synechocystis sp. PCC 6803 for Production of the Plant Diterpenoid Manoyl Oxide. ACS Synth Biol. 2015 Jul 13.
pEERM3 KmPlasmid for integration into neutral site of Synechocystis 6803Bacterial Expression, Synthetic Biology Lindberg Metabolic Engineering of Synechocystis sp. PCC 6803 for Production of the Plant Diterpenoid Manoyl Oxide. ACS Synth Biol. 2015 Jul 13.
pEERM4 CmPlasmid for integration into neutral site 2 of Synechocystis 6803Bacterial Expression, Synthetic Biology Lindberg Metabolic Engineering of Synechocystis sp. PCC 6803 for Production of the Plant Diterpenoid Manoyl Oxide. ACS Synth Biol. 2015 Jul 13.
pEERM6 KmPlasmid for integration into sqs site of Synechocystis 6803Bacterial Expression, Synthetic Biology Lindberg Metabolic Engineering of Synechocystis sp. PCC 6803 for Production of the Plant Diterpenoid Manoyl Oxide. ACS Synth Biol. 2015 Jul 13.
pJexpress414_OmpA171_H1237OmpA redesign hybrid with wild-type strands 1237OmpA_H1237 (Synthetic)Bacterial Expression, Synthetic Biology Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
pJexpress414_OmpA171_H2578OmpA redesign hybrid with wild-type strands 2578OmpA_H2578 (Synthetic)Bacterial Expression, Synthetic Biology Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
pJexpress414_OmpA171_H3478OmpA redesign hybrid with wild-type strands 3478OmpA_H3478 (Synthetic)Bacterial Expression, Synthetic Biology Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
pJexpress414_OmpA171_H1248OmpA redesign hybrid with wild-type strands 1248OmpA_H1248 (Synthetic)Bacterial Expression, Synthetic Biology Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
pJexpress414_OmpA171_H2467OmpA redesign hybrid with wild-type strands 2467OmpA_H2467 (Synthetic)Bacterial Expression, Synthetic Biology Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
pJexpress414_OmpA171_H1457OmpA redesign hybrid with wild-type strands 1457OmpA_H1457 (Synthetic)Bacterial Expression, Synthetic Biology Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
pJexpress414_OmpA171_H4568OmpA redesign hybrid with wild-type strands 4568OmpA_H4568 (Synthetic)Bacterial Expression, Synthetic Biology Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
pJexpress414_OmpA171_H1358OmpA redesign hybrid with wild-type strands 1358OmpA_H1358 (Synthetic)Bacterial Expression, Synthetic Biology Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
pJexpress414_OmpA171_R4cons_G125LConservative version of OmpA Redesign 4 with G125L mutationOmpA_R4cons_G125L (Synthetic)Bacterial Expression, Synthetic Biology Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
pJexpress414_OmpA171_R4cons_G41AConservative version of OmpA Redesign 4 with G41A mutationOmpA_R4cons_G41A (Synthetic)Bacterial Expression, Synthetic Biology Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
pJexpress414_OmpA171_R4cons_G99IConservative version of OmpA Redesign 4 with G99I mutationOmpA_R4cons_G99I (Synthetic)Bacterial Expression, Synthetic Biology Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
pJexpress414_OmpA171_R4cons_P121FConservative version of OmpA Redesign 4 with P121F mutationOmpA_R4cons_P121F (Synthetic)Bacterial Expression, Synthetic Biology Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
pJexpress414_OmpA171_R4cons_Y129HConservative version of OmpA Redesign 4 with Y129H mutationOmpA_R4cons_Y129H (Synthetic)Bacterial Expression, Synthetic Biology Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
pJexpress414_OmpA171_H-3OmpA redesign hybrid with wild-type strands 1245678OmpA_H-3 (Synthetic)Bacterial Expression, Synthetic Biology Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
pJexpress414_OmpA171_H-46OmpA redesign hybrid with wild-type strands 123578OmpA_H-46 (Synthetic)Bacterial Expression, Synthetic Biology Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
pJexpress414_OmpA171_H346OmpA redesign hybrid with wild-type strands 346OmpA_H346 (Synthetic)Bacterial Expression, Synthetic Biology Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
pJexpress414_OmpA171_H-78OmpA redesign hybrid with wild-type strands 123456OmpA_H-78 (Synthetic)Bacterial Expression, Synthetic Biology Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
pDCAF3-IS5Expresses N-demethylases for the degredation of caffeine and related methylxanthines. Can be used to measure caffeine content as described in the publication listed below.ndmA (Other), ndmB (Other), ndmC (Other), ndmD (Other), gst9 (Other), chlR (Other)Bacterial Expression, Synthetic Biology Barrick Decaffeination and measurement of caffeine content by addicted Escherichia coli with a refactored N-demethylation operon from Pseudomonas putida CBB5. ACS Synth Biol. 2013 Jun 21;2(6):301-7. doi: 10.1021/sb4000146. Epub 2013 Mar 22.
pKS012pSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing (N to C terminal) Ntag and mCherryNtag-mCherryBacterial Expression, Synthetic Biology Tyo N-Terminal-Based Targeted, Inducible Protein Degradation in Escherichia coli. PLoS One. 2016 Feb 22;11(2):e0149746. doi: 10.1371/journal.pone.0149746. eCollection 2016.
pKS013pSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing mCherrymCherryBacterial Expression, Synthetic Biology Tyo N-Terminal-Based Targeted, Inducible Protein Degradation in Escherichia coli. PLoS One. 2016 Feb 22;11(2):e0149746. doi: 10.1371/journal.pone.0149746. eCollection 2016.
pKS014pSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing (N to C terminal) Ntag and beta-galactosidaseNtag-beta galactosidaseBacterial Expression, Synthetic Biology Tyo N-Terminal-Based Targeted, Inducible Protein Degradation in Escherichia coli. PLoS One. 2016 Feb 22;11(2):e0149746. doi: 10.1371/journal.pone.0149746. eCollection 2016.
pJB005pTrc plasmid expressing GFPGFPBacterial Expression, Synthetic Biology Tyo N-Terminal-Based Targeted, Inducible Protein Degradation in Escherichia coli. PLoS One. 2016 Feb 22;11(2):e0149746. doi: 10.1371/journal.pone.0149746. eCollection 2016.
pJB028pTrc plasmid expressing p14*-TEVp14*-TEVBacterial Expression, Synthetic Biology Tyo N-Terminal-Based Targeted, Inducible Protein Degradation in Escherichia coli. PLoS One. 2016 Feb 22;11(2):e0149746. doi: 10.1371/journal.pone.0149746. eCollection 2016.
pKS042pTrc plasmid expressing clpAclpABacterial Expression, Synthetic Biology Tyo N-Terminal-Based Targeted, Inducible Protein Degradation in Escherichia coli. PLoS One. 2016 Feb 22;11(2):e0149746. doi: 10.1371/journal.pone.0149746. eCollection 2016.
pKS043pTrc plasmid expressing clpSclpSBacterial Expression, Synthetic Biology Tyo N-Terminal-Based Targeted, Inducible Protein Degradation in Escherichia coli. PLoS One. 2016 Feb 22;11(2):e0149746. doi: 10.1371/journal.pone.0149746. eCollection 2016.
pKS044pTrc plasmid expressing clpPclpPBacterial Expression, Synthetic Biology Tyo N-Terminal-Based Targeted, Inducible Protein Degradation in Escherichia coli. PLoS One. 2016 Feb 22;11(2):e0149746. doi: 10.1371/journal.pone.0149746. eCollection 2016.
PAS501 (TJF040)pETDuet-1 Containing FadD Q338RFadD with Q338R mutation (Other)Bacterial Expression Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS500 (TJF032)pETDuet-1 Containing WT FadDFadD (Other)Bacterial Expression Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS502 (TJF065)pETDuet-1 Containing FadD H376RFadD (Other)Bacterial Expression Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS503 (TJF066)pETDuet-1 Containing FadD V4F, W5LFadD with V4F, W5L mutations (Other)Bacterial Expression Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS504 (TJF067)pETDuet-1 Containing FadD F447SFadD with F447S mutation (Other)Bacterial Expression Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS505 (TJF068)pETDuet-1 Containing FadD V451AFadD with V451A mutation (Other)Bacterial Expression Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS506 (TJF069)pETDuet-1 Containing FadD D372GFadD D372G (Other)Bacterial Expression Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS507 (TJF070)pETDuet-1 Containing FadD Y9HFadD (Other)Bacterial Expression Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS508 (TJF071)pETDuet-1 Containing FadD T349AFadD (Other)Bacterial Expression Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS509 (TJF032 L448S)pETDuet-1 Containing FadD L448SFadD (Other)Bacterial Expression Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS510 (TJF032 G446S)pETDuet-1 Containing FadD G446SFadD (Other)Bacterial Expression Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS511 (TJF032 Y375R)pETDuet-1 Containing FadD Y375RFadD (Other)Bacterial Expression Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS512 (TJF032 S377R)pETDuet-1 Containing FadD S377RFadD (Other)Bacterial Expression Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS513 (TJF080)pETDuet-1 Containing FadD S379RFadD (Other)Bacterial Expression Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS514 (TJF081)pETDuet-1 Containing FadD F447DFadD (Other)Bacterial Expression Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS515 (TJF082)pETDuet-1 Containing FadD F447RFadD (Other)Bacterial Expression Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS516 (TJF083)pETDuet-1 Containing FadD F447R S379RFadD (Other)Bacterial Expression Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS517 (TJF084)pETDuet-1 Containing FadD F447D S379RFadD (Other)Bacterial Expression Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS518 (TJF085)pETDuet-1 Containing FadD R554GFadD (Other)Bacterial Expression Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS519 (TJF086)pETDuet-1 Containing FadD D551GFadD (Other)Bacterial Expression Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS520 (TJF087)pETDuet-1 Containing FadD Q338GFadD (Other)Bacterial Expression Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS521 (TJF088)pETDuet-1 Containing FadD R449GFadD (Other)Bacterial Expression Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS522 (TJF089)pETDuet-1 Containing FadD V451GFadD (Other)Bacterial Expression Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS523 (TJF090)pETDuet-1 Containing FadD H376GFadD (Other)Bacterial Expression Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS524 (TJF091)pETDuet-1 Containing FadD S379GFadD (Other)Bacterial Expression Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS525 (TJF092)pETDuet-1 Containing FadD Q339GFadD (Other)Bacterial Expression Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS526 (TJF093)pETDuet-1 Containing FadD L459GFadD (Other)Bacterial Expression Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS527 (TJF094)pETDuet-1 Containing FadD Y371GFadD (Other)Bacterial Expression Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS528 (TJF059)pETDuet-1 Containing FadD-HisFadD (Other)Bacterial Expression Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS529 (TJF072)pETDuet-1 Containing FadD Q338R-HisFadD (Other)Bacterial Expression Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS530 (TJF073)pETDuet-1 Containing FadD H376R-HisFadD (Other)Bacterial Expression Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS531 (TJF074)pETDuet-1 Containing FadD V4F, W5L -HisFadD (Other)Bacterial Expression Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS532 (TJF075)pETDuet-1 Containing FadD F447S-HisFadD (Other)Bacterial Expression Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS533 (TJF076)pETDuet-1 Containing FadD V451A-HisFadD (Other)Bacterial Expression Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS534 (TJF077)pETDuet-1 Containing FadD D372G-HisFadD (Other)Bacterial Expression Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS535 (TJF078)pETDuet-1 Containing FadD Y9H-HisFadD (Other)Bacterial Expression Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS536 (TJF079)pETDuet-1 Containing FadD T349A-HisFadD (Other)Bacterial Expression Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
pCAPB02A transformation-associated recombination (TAR) based genomic capture and expression vector for antibiotic production in B. subtilisBacterial Expression, Synthetic Biology Qian Directed natural product biosynthesis gene cluster capture and expression in the model bacterium Bacillus subtilis. Sci Rep. 2015 Mar 24;5:9383. doi: 10.1038/srep09383.
J23116_FBMoClo Basic Part: Constitutive promoter - Anderson series - low strength [F:J23116:B]Constitutive promoter (Synthetic)Synthetic Biology Densmore CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
Burden Monitor lambda versionThe burden monitor was cloned inside plasmid pAH63 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cellsGFP Ellis Quantifying cellular capacity identifies gene expression designs with reduced burden. Nat Methods. 2015 May;12(5):415-8. doi: 10.1038/nmeth.3339. Epub 2015 Apr 6.
Burden Monitor phi80 versionThe burden monitor was cloned inside plasmid pAH153 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cellGFP Ellis Quantifying cellular capacity identifies gene expression designs with reduced burden. Nat Methods. 2015 May;12(5):415-8. doi: 10.1038/nmeth.3339. Epub 2015 Apr 6.
pInt-tsThe pInt-ts plasmid [Haldimann2001] is the lambda helper plasmid necessary for the genome integration of the burden monitor lambda versionlambda integrase Ellis Quantifying cellular capacity identifies gene expression designs with reduced burden. Nat Methods. 2015 May;12(5):415-8. doi: 10.1038/nmeth.3339. Epub 2015 Apr 6.
pAH123The pAH123 plasmid [Haldimann2001] is the phi80 helper plasmid necessary for the genome integration of the burden monitor phi80 versionphi80 integrase Ellis Quantifying cellular capacity identifies gene expression designs with reduced burden. Nat Methods. 2015 May;12(5):415-8. doi: 10.1038/nmeth.3339. Epub 2015 Apr 6.
pETM6-C4-mCherryExpresses mCherry under the control of Mutant T7 Promoter C4mCherry (Synthetic)Bacterial Expression, Synthetic Biology Koffas ePathOptimize: A Combinatorial Approach for Transcriptional Balancing of Metabolic Pathways. Sci Rep. 2015 Jun 11;5:11301. doi: 10.1038/srep11301.
pETM6-H10-mCherryExpresses mCherry under the control of Mutant T7 Promoter H10mCherry (Synthetic)Bacterial Expression, Synthetic Biology Koffas ePathOptimize: A Combinatorial Approach for Transcriptional Balancing of Metabolic Pathways. Sci Rep. 2015 Jun 11;5:11301. doi: 10.1038/srep11301.
pETM6-H9-mCherryExpresses mCherry under the control of Mutant T7 Promoter H9mCherry (Synthetic)Bacterial Expression, Synthetic Biology Koffas ePathOptimize: A Combinatorial Approach for Transcriptional Balancing of Metabolic Pathways. Sci Rep. 2015 Jun 11;5:11301. doi: 10.1038/srep11301.
pETM6-G6-mCherryExpresses mCherry under the control of Mutant T7 Promoter G6mCherry (Synthetic)Bacterial Expression, Synthetic Biology Koffas ePathOptimize: A Combinatorial Approach for Transcriptional Balancing of Metabolic Pathways. Sci Rep. 2015 Jun 11;5:11301. doi: 10.1038/srep11301.
pETM6-mCherryExpresses mCherry under the control of the Concensus T7 PromotermCherry (Synthetic)Bacterial Expression, Synthetic Biology Koffas ePathOptimize: A Combinatorial Approach for Transcriptional Balancing of Metabolic Pathways. Sci Rep. 2015 Jun 11;5:11301. doi: 10.1038/srep11301.
pETM6-vioABECDpETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with consensus promoterVioA (Other), VioB (Other), VioE (Other), VioC (Other), VioD (Other)Bacterial Expression, Synthetic Biology Koffas ePathOptimize: A Combinatorial Approach for Transcriptional Balancing of Metabolic Pathways. Sci Rep. 2015 Jun 11;5:11301. doi: 10.1038/srep11301.
pETM6-G6-vioABECDpETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with G6 promoterVioA (Other), VioB (Other), VioE (Other), VioC (Other), VioD (Other)Bacterial Expression, Synthetic Biology Koffas ePathOptimize: A Combinatorial Approach for Transcriptional Balancing of Metabolic Pathways. Sci Rep. 2015 Jun 11;5:11301. doi: 10.1038/srep11301.
pETM6-E12-vioABECDpETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with optimized promoters (E12)VioA (Other), VioB (Other), VioE (Other), VioC (Other), VioD (Other)Bacterial Expression, Synthetic Biology Koffas ePathOptimize: A Combinatorial Approach for Transcriptional Balancing of Metabolic Pathways. Sci Rep. 2015 Jun 11;5:11301. doi: 10.1038/srep11301.
pETM6-M.accABCDEncodes accA, accB, accC, accD in monocistronic configuration.accA (Other), accB (Other), accC (Other), accD (Other)Bacterial Expression, Synthetic Biology Koffas Modular optimization of multi-gene pathways for fatty acids production in E. coli. Nat Commun. 2013;4:1409. doi: 10.1038/ncomms2425.
pETM6-P.accABCDEncodes accA, accB, accC, accD in Pseudo-operon configuration.accA (Other), accB (Other), accC (Other), accD (Other)Bacterial Expression, Synthetic Biology Koffas Modular optimization of multi-gene pathways for fatty acids production in E. coli. Nat Commun. 2013;4:1409. doi: 10.1038/ncomms2425.
pRAV12Parental plasmid for transcription of RNAs with a HdV/M-domain 3'-tag for native purificationTranscription cassette for 3'-tagged RNAs (Synthetic)Bacterial Expression, Synthetic Biology Batey A general method for rapid and nondenaturing purification of RNAs. RNA. 2004 Jun;10(6):988-95.
pTmaM4Expression vector hexahistidine tagged variant of the Thermotoga maritima M-domain proteinThermotoga marítima Ffh-M domain (Other)Bacterial Expression Batey A general method for rapid and nondenaturing purification of RNAs. RNA. 2004 Jun;10(6):988-95.
Pr-deGFP-MGaptOR2-OR1-Pr promoter expressing deGFP-MG aptamer fusionUTR1-deGFP-MGapt-T500 (Synthetic)Bacterial Expression, Synthetic Biology Murray Gene circuit performance characterization and resource usage in a cell-free "breadboard". ACS Synth Biol. 2014 Jun 20;3(6):416-25. doi: 10.1021/sb400203p. Epub 2014 Apr 7.
Pr1-deGFP-MGaptOR2-OR1-Pr1 promoter expressing deGFP-MG aptamer fusionUTR1-deGFP-MGapt-T500 (Synthetic)Bacterial Expression, Synthetic Biology Murray Gene circuit performance characterization and resource usage in a cell-free "breadboard". ACS Synth Biol. 2014 Jun 20;3(6):416-25. doi: 10.1021/sb400203p. Epub 2014 Apr 7.
Pr2-deGFP-MGaptOR2-OR1-Pr2 promoter expressing deGFP-MG aptamer fusionUTR1-deGFP-MGapt-T500 (Synthetic)Bacterial Expression, Synthetic Biology Murray Gene circuit performance characterization and resource usage in a cell-free "breadboard". ACS Synth Biol. 2014 Jun 20;3(6):416-25. doi: 10.1021/sb400203p. Epub 2014 Apr 7.
Pr1-T7RNAPOR2-OR1-Pr1 promoter expressing T7 RNAPUTR1-T7RNAP-T500 (Synthetic)Bacterial Expression, Synthetic Biology Murray Gene circuit performance characterization and resource usage in a cell-free "breadboard". ACS Synth Biol. 2014 Jun 20;3(6):416-25. doi: 10.1021/sb400203p. Epub 2014 Apr 7.
Pr2-T7RNAPOR2-OR1-P2 promoter expressing T7 RNAPUTR1-T7RNAP-T500 (Synthetic)Bacterial Expression, Synthetic Biology Murray Gene circuit performance characterization and resource usage in a cell-free "breadboard". ACS Synth Biol. 2014 Jun 20;3(6):416-25. doi: 10.1021/sb400203p. Epub 2014 Apr 7.
Pr-deCFP-MGaptOR2-OR1-Pr promoter expressing deCFP-MG aptamer fusionUTR1-deCFP-MGapt-T500 (Synthetic)Bacterial Expression, Synthetic Biology Murray Gene circuit performance characterization and resource usage in a cell-free "breadboard". ACS Synth Biol. 2014 Jun 20;3(6):416-25. doi: 10.1021/sb400203p. Epub 2014 Apr 7.
PT7-deGFP-MGaptT7 promoter expressing deGFP-MG aptamer fusionUTR1-deGFP-MGapt-T7term (Synthetic)Bacterial Expression, Synthetic Biology Murray Gene circuit performance characterization and resource usage in a cell-free "breadboard". ACS Synth Biol. 2014 Jun 20;3(6):416-25. doi: 10.1021/sb400203p. Epub 2014 Apr 7.
Pr-deGFPPr promoter expressing deGFPUTR1-deGFP-T500 (Synthetic)Bacterial Expression, Synthetic Biology Murray Gene circuit performance characterization and resource usage in a cell-free "breadboard". ACS Synth Biol. 2014 Jun 20;3(6):416-25. doi: 10.1021/sb400203p. Epub 2014 Apr 7.
Pr-delRBS-deGFPPr promoter expressing a deGFP that is missing a ribosomal binding sitedeGFP-T500 (Synthetic)Bacterial Expression, Synthetic Biology Murray Gene circuit performance characterization and resource usage in a cell-free "breadboard". ACS Synth Biol. 2014 Jun 20;3(6):416-25. doi: 10.1021/sb400203p. Epub 2014 Apr 7.
BASIC_6_J23102storage vector for BASIC part: J23102J23102 Anderson PromoterBacterial Expression Baldwin BASIC: A New Biopart Assembly Standard for Idempotent Cloning Provides Accurate, Single-Tier DNA Assembly for Synthetic Biology. ACS Synth Biol. 2015 Apr 16.
SepOTSβ Phosphoserine orthogonal translation systemSepRS/EF-Sep, 1xtRNA-Sep (Synthetic)Bacterial Expression, Synthetic Biology Rinehart A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation. Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130.
SepOTSγPhosphoserine orthogonal translation systemSepRS9/EF-Sep21, 1xtRNA-Sep (Synthetic)Bacterial Expression, Synthetic Biology Rinehart A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation. Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130.
SepOTSδPhosphoserine orthogonal translation systemSepRS/EF-Sep, 1xtRNA-Sep-A37 (Synthetic)Bacterial Expression, Synthetic Biology Rinehart A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation. Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130.
SepOTSεPhosphoserine orthogonal translation systemSepRS9/EF-Sep21, 1xtRNA-Sep-A37 (Synthetic)Bacterial Expression, Synthetic Biology Rinehart A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation. Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130.
SepOTSζPhosphoserine orthogonal translation systemSepRS/EF-Sep, 2xtRNA-Sep (Synthetic)Bacterial Expression, Synthetic Biology Rinehart A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation. Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130.
SepOTSηPhosphoserine orthogonal translation systemSepRS9/EF-Sep21, 2xtRNA-Sep (Synthetic)Bacterial Expression, Synthetic Biology Rinehart A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation. Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130.
SepOTSθPhosphoserine orthogonal translation systemSepRS/EF-Sep, 2xtRNA-Sep-A37 (Synthetic)Bacterial Expression, Synthetic Biology Rinehart A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation. Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130.
SepOTSιPhosphoserine orthogonal translation systemSepRS9/EFSep21, 2xtRNA-Sep-A37 (Synthetic)Bacterial Expression, Synthetic Biology Rinehart A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation. Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130.
SepOTSλ (recommended)Phosphoserine orthogonal translation system (preferred variant)SepRS9/EFSep21, 4xtRNA-Sep-A37 (Synthetic)Bacterial Expression, Synthetic Biology Rinehart A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation. Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130.
SepOTSνPhosphoserine orthogonal translation systemSepRS9/EF-Sep21, 5xtRNA-Sep (Synthetic)Bacterial Expression, Synthetic Biology Rinehart A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation. Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130.
Beta lactamase S68TAGBeta lactamase S68TAG geneS68TAG beta lactamase (Synthetic)Bacterial Expression, Synthetic Biology Rinehart A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation. Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130.
SupDPlasmid encoding tRNA-SupD for serine amber suppression2xtRNA-SupDBacterial Expression, Synthetic Biology Rinehart A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation. Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130.
pSCKbreak12pSTC2,cisRaj12-sfGFP, KanRCisRaj12-sfGFP (Synthetic)Bacterial Expression, Synthetic Biology Jaramillo Dynamic signal processing by ribozyme-mediated RNA circuits to control gene expression. Nucleic Acids Res. 2015 May 26;43(10):5158-70. doi: 10.1093/nar/gkv287. Epub 2015 Apr 27.
pSCKtheoRaj11pSTC1,TheoHHAzRaj11,cisRaj11-sfGFP, kanRTheoHHAzRaj11,cisRaj11,sfGFP (Synthetic)Bacterial Expression, Synthetic Biology Jaramillo Dynamic signal processing by ribozyme-mediated RNA circuits to control gene expression. Nucleic Acids Res. 2015 May 26;43(10):5158-70. doi: 10.1093/nar/gkv287. Epub 2015 Apr 27.
pSCKtheoRaj12-olsapSTC2,theoHHAzRaj12,cisRaj12-sfGFP, KanRTheoHHAzRaj12,cisRaj12,sfGFP (Synthetic), theoHHAzRaj12,cisRaj12-sfGFP (Synthetic)Bacterial Expression, Synthetic Biology Jaramillo Dynamic signal processing by ribozyme-mediated RNA circuits to control gene expression. Nucleic Acids Res. 2015 May 26;43(10):5158-70. doi: 10.1093/nar/gkv287. Epub 2015 Apr 27.
pSCKtheoRaj12-o11pSTC2,theoHHAzRaj12,cisRaj11-sfGFP, KanRTheoHHAzRaj12,cisRaj11,sfGFP (Synthetic)Bacterial Expression, Synthetic Biology Jaramillo Dynamic signal processing by ribozyme-mediated RNA circuits to control gene expression. Nucleic Acids Res. 2015 May 26;43(10):5158-70. doi: 10.1093/nar/gkv287. Epub 2015 Apr 27.
pSCKtheoRaj12pSTC2,TheoHHAzRaj12,cisRaj12-sfGFP, kanRTheoHHAzRaj12,cisRaj12,sfGFP (Synthetic)Synthetic Biology Jaramillo Dynamic signal processing by ribozyme-mediated RNA circuits to control gene expression. Nucleic Acids Res. 2015 May 26;43(10):5158-70. doi: 10.1093/nar/gkv287. Epub 2015 Apr 27.
pSCKtheoRaj12mpSTC2,TheoHHAzRaj12mut,cisRaj12-sfGFP, kanRpSCKtheoRaj12m (Synthetic)Synthetic Biology ; pSC101 origin of replication Jaramillo Dynamic signal processing by ribozyme-mediated RNA circuits to control gene expression. Nucleic Acids Res. 2015 May 26;43(10):5158-70. doi: 10.1093/nar/gkv287. Epub 2015 Apr 27.
pENV8(WT)-GFPuvCobalamin riboswitch reporter vector for use in E. coliwild type environmental cobalamin riboswitch sequence (env8) (Other)Bacterial Expression, Synthetic Biology Batey B12 cofactors directly stabilize an mRNA regulatory switch. Nature. 2012 Dec 6;492(7427):133-7. doi: 10.1038/nature11607. Epub 2012 Oct 14.
pENV8(GAAA)-GFPuvNegative control cobalamin riboswitch reporter vector for use in E. coli; deletion of regulatory kissing loop interaction by conversion of L5 to GAAA tetra loopkissing loop regulatory mutant of environmental cobalamin riboswitch sequence (env8) (Other)Bacterial Expression, Synthetic Biology Batey B12 cofactors directly stabilize an mRNA regulatory switch. Nature. 2012 Dec 6;492(7427):133-7. doi: 10.1038/nature11607. Epub 2012 Oct 14.
pWKS1583Modular vector for secreted amylase reporter expressionamyEopt (Synthetic)Bacterial Expression, Synthetic Biology Smits The Signal Sequence of the Abundant Extracellular Metalloprotease PPEP-1 Can Be Used to Secrete Synthetic Reporter Proteins in Clostridium difficile. ACS Synth Biol. 2016 Jun 23.
pAP24Modular vector for secreted luciferase reporter expressionsLucopt (Synthetic)Bacterial Expression, Synthetic Biology Smits The Signal Sequence of the Abundant Extracellular Metalloprotease PPEP-1 Can Be Used to Secrete Synthetic Reporter Proteins in Clostridium difficile. ACS Synth Biol. 2016 Jun 23.
pBEST_OR2-OR1-Pr-UTR1_ClpX-T500 (48p)Derived from 21 with “ClpX” coding sequence from Genbank U00096.3 substituted for “deGFP” – used as T500 template for 134-137.ClpX Murray Linear DNA for rapid prototyping of synthetic biological circuits in an Escherichia coli based TX-TL cell-free system. ACS Synth Biol. 2014 Jun 20;3(6):387-97. doi: 10.1021/sb400131a. Epub 2013 Dec 4.
pBEST_OR2-OR1-Pr_UTR1-deGFP-T500 BsaI, BbsI-safe (105p)Derived from 21 with silent mutations to make BsaI, BbsI compatible deGFP Murray Linear DNA for rapid prototyping of synthetic biological circuits in an Escherichia coli based TX-TL cell-free system. ACS Synth Biol. 2014 Jun 20;3(6):387-97. doi: 10.1021/sb400131a. Epub 2013 Dec 4.
pBEST-Pl-tetO1-tetR-linker-deGFP-T500 (109p)Post cloned 4-piece from Isothermal/Golden Gate assembly (GGA), using sequences from 2 (PltetO1-UTR1), 3 (tetR+linker from primer extension), 21 (linker from primer extension + deGFP), and 105 (vectortetR-deGFP fusion Murray Linear DNA for rapid prototyping of synthetic biological circuits in an Escherichia coli based TX-TL cell-free system. ACS Synth Biol. 2014 Jun 20;3(6):387-97. doi: 10.1021/sb400131a. Epub 2013 Dec 4.
pBEST-p15A-OR2-OR1-Pr-UTR1-deGFPT500 (121p)Promoter paneldeGFP Murray Linear DNA for rapid prototyping of synthetic biological circuits in an Escherichia coli based TX-TL cell-free system. ACS Synth Biol. 2014 Jun 20;3(6):387-97. doi: 10.1021/sb400131a. Epub 2013 Dec 4.
pBEST-p15A-Pl-tetO1-deGFP-T500 (122p)Promoter paneldeGFP Murray Linear DNA for rapid prototyping of synthetic biological circuits in an Escherichia coli based TX-TL cell-free system. ACS Synth Biol. 2014 Jun 20;3(6):387-97. doi: 10.1021/sb400131a. Epub 2013 Dec 4.
pBEST-p15A-Pl-lacO1-deGFP-T500 (123p)Promoter paneldeGFP Murray Linear DNA for rapid prototyping of synthetic biological circuits in an Escherichia coli based TX-TL cell-free system. ACS Synth Biol. 2014 Jun 20;3(6):387-97. doi: 10.1021/sb400131a. Epub 2013 Dec 4.
pBEST-p15A-J23113-deGFP-T500 (124p)Promoter paneldeGFP Murray Linear DNA for rapid prototyping of synthetic biological circuits in an Escherichia coli based TX-TL cell-free system. ACS Synth Biol. 2014 Jun 20;3(6):387-97. doi: 10.1021/sb400131a. Epub 2013 Dec 4.
pBEST-p15A-J23114-deGFP-T500 (125p)Promoter paneldeGFP Murray Linear DNA for rapid prototyping of synthetic biological circuits in an Escherichia coli based TX-TL cell-free system. ACS Synth Biol. 2014 Jun 20;3(6):387-97. doi: 10.1021/sb400131a. Epub 2013 Dec 4.
pBEST-p15A-J23116-deGFP-T500 (126p)Promoter paneldeGFP Murray Linear DNA for rapid prototyping of synthetic biological circuits in an Escherichia coli based TX-TL cell-free system. ACS Synth Biol. 2014 Jun 20;3(6):387-97. doi: 10.1021/sb400131a. Epub 2013 Dec 4.
pBEST-p15A-J23150-deGFP-T500 (127p)Promoter paneldeGFP Murray Linear DNA for rapid prototyping of synthetic biological circuits in an Escherichia coli based TX-TL cell-free system. ACS Synth Biol. 2014 Jun 20;3(6):387-97. doi: 10.1021/sb400131a. Epub 2013 Dec 4.
pBEST-p15A-J23106-deGFP-T500 (128p)Promoter paneldeGFP Murray Linear DNA for rapid prototyping of synthetic biological circuits in an Escherichia coli based TX-TL cell-free system. ACS Synth Biol. 2014 Jun 20;3(6):387-97. doi: 10.1021/sb400131a. Epub 2013 Dec 4.
pBEST-p15A-J23151-deGFP-T500 (129p)Promoter paneldeGFP Murray Linear DNA for rapid prototyping of synthetic biological circuits in an Escherichia coli based TX-TL cell-free system. ACS Synth Biol. 2014 Jun 20;3(6):387-97. doi: 10.1021/sb400131a. Epub 2013 Dec 4.
pBEST-p15A-J23100-deGFP-T500 (130p)Promoter paneldeGFP Murray Linear DNA for rapid prototyping of synthetic biological circuits in an Escherichia coli based TX-TL cell-free system. ACS Synth Biol. 2014 Jun 20;3(6):387-97. doi: 10.1021/sb400131a. Epub 2013 Dec 4.
pBEST-p15A-J23101-deGFP-T500 (131p)Promoter paneldeGFP Murray Linear DNA for rapid prototyping of synthetic biological circuits in an Escherichia coli based TX-TL cell-free system. ACS Synth Biol. 2014 Jun 20;3(6):387-97. doi: 10.1021/sb400131a. Epub 2013 Dec 4.
pBEST-p15A-J23102-deGFP-T500 (132p)Promoter paneldeGFP Murray Linear DNA for rapid prototyping of synthetic biological circuits in an Escherichia coli based TX-TL cell-free system. ACS Synth Biol. 2014 Jun 20;3(6):387-97. doi: 10.1021/sb400131a. Epub 2013 Dec 4.
pBEST-p15A-pNull-deGFP-T500 (133p)Promoter paneldeGFP Murray Linear DNA for rapid prototyping of synthetic biological circuits in an Escherichia coli based TX-TL cell-free system. ACS Synth Biol. 2014 Jun 20;3(6):387-97. doi: 10.1021/sb400131a. Epub 2013 Dec 4.
P3U2C7T2-v1-1 (Pl-lacO1-deGFP) (134p)1 Part of 4-part switchdeGFP Murray Linear DNA for rapid prototyping of synthetic biological circuits in an Escherichia coli based TX-TL cell-free system. ACS Synth Biol. 2014 Jun 20;3(6):387-97. doi: 10.1021/sb400131a. Epub 2013 Dec 4.
P4U2C8T2-v1-1 (Pl-tetO1-deCFP) (135p)1 Part of 4-part switchdeCFP Murray Linear DNA for rapid prototyping of synthetic biological circuits in an Escherichia coli based TX-TL cell-free system. ACS Synth Biol. 2014 Jun 20;3(6):387-97. doi: 10.1021/sb400131a. Epub 2013 Dec 4.
P3U2C5T2-v1-2 (Pl-lacO1-tetR) (136p)1 Part of 4-part switchtetR Murray Linear DNA for rapid prototyping of synthetic biological circuits in an Escherichia coli based TX-TL cell-free system. ACS Synth Biol. 2014 Jun 20;3(6):387-97. doi: 10.1021/sb400131a. Epub 2013 Dec 4.
P4U2C6T2-v1-2 (Pl-tetO1-lacI) (137p)1 Part of 4-part switchlacI Murray Linear DNA for rapid prototyping of synthetic biological circuits in an Escherichia coli based TX-TL cell-free system. ACS Synth Biol. 2014 Jun 20;3(6):387-97. doi: 10.1021/sb400131a. Epub 2013 Dec 4.
pRL439Source of psbA promoter of Amaranthus hybridus, used as a strong promoter of cyanobacterial genespsbA promoter (Other)Bacterial Expression Wolk A versatile class of positive-selection vectors based on the nonviability of palindrome-containing plasmids that allows cloning into long polylinkers. Gene. 1988 Aug 15;68(1):119-38.
pET21a(+)-Histag-Cerulean (526) Expression vector for Cerulean purificationCerulean Murray Rapid cell-free forward engineering of novel genetic ring oscillators. Elife. 2015 Oct 2;4. doi: 10.7554/eLife.09771.
pET21a(+)-Histag-Citrine (525)Expression vector for Citrine purificationCitrine Murray Rapid cell-free forward engineering of novel genetic ring oscillators. Elife. 2015 Oct 2;4. doi: 10.7554/eLife.09771.
pET21a(+)-Histag-mCherry (527)Expression vector for mCherry purificationmCherry Murray Rapid cell-free forward engineering of novel genetic ring oscillators. Elife. 2015 Oct 2;4. doi: 10.7554/eLife.09771.
pBetI-BCD7-Citrine P69U18C68T11 v40-1 (532)Citrine reporter for transfer fxnsCitrine Murray Rapid cell-free forward engineering of novel genetic ring oscillators. Elife. 2015 Oct 2;4. doi: 10.7554/eLife.09771.
pLacI-BCD7-Citrine P42U18C68T11 v40-1 (536)Citrine reporter for transfer fxnsCitrine Murray Rapid cell-free forward engineering of novel genetic ring oscillators. Elife. 2015 Oct 2;4. doi: 10.7554/eLife.09771.
pCI-BCD7-Citrine P62U18C68T11 v40-1 (523)Citrine reporter for transfer fxnsCitrine Murray Rapid cell-free forward engineering of novel genetic ring oscillators. Elife. 2015 Oct 2;4. doi: 10.7554/eLife.09771.
3n1 (572)BetI/PhlF/SrpR BCD7 3-node v41-1srpR-ssrA, betI-ssrA, phlF-ssrA Murray Rapid cell-free forward engineering of novel genetic ring oscillators. Elife. 2015 Oct 2;4. doi: 10.7554/eLife.09771.
5n1 (560)TetR/PhlF/SrpR/LambdaCI/LacI BCD7 5-node v41-2srpR-ssrA, lambdaCI-ssrA, phlF-ssrA, lacI-ssrA, tetR-ssrA Murray Rapid cell-free forward engineering of novel genetic ring oscillators. Elife. 2015 Oct 2;4. doi: 10.7554/eLife.09771.
pBetI-BCD7-phlF-ssrA P69U18C51T15 v40-3 (570)plasmid for building 3n1phlF-ssrA Murray Rapid cell-free forward engineering of novel genetic ring oscillators. Elife. 2015 Oct 2;4. doi: 10.7554/eLife.09771.
pBetI-BCD7-qacR-ssrA P69U18C63T16 v50-2 (568)plasmid for building 5n2phlF-ssrA Murray Rapid cell-free forward engineering of novel genetic ring oscillators. Elife. 2015 Oct 2;4. doi: 10.7554/eLife.09771.
pLacO1-BCD7-tetR-ssrA P42U18C45T13 v40-5a (497)plasmid for building 5n1tetR-ssrA Murray Rapid cell-free forward engineering of novel genetic ring oscillators. Elife. 2015 Oct 2;4. doi: 10.7554/eLife.09771.
pSrpR-BCD7-betI-ssrA P65U18C64T14 v50-2 (567)plasmid for building 3n1, 5n2betI-ssrA Murray Rapid cell-free forward engineering of novel genetic ring oscillators. Elife. 2015 Oct 2;4. doi: 10.7554/eLife.09771.
pSrpR-BCD7-lambdaCI-ssrA P65U18C47T14 v40-2 (495)plasmid for building 5n1lambdaCI-ssrA Murray Rapid cell-free forward engineering of novel genetic ring oscillators. Elife. 2015 Oct 2;4. doi: 10.7554/eLife.09771.
pSrpR-BCD7-tetR-ssrA P65U18C45T14 v40-2 (491)plasmid for building 3n2tetR-ssrA Murray Rapid cell-free forward engineering of novel genetic ring oscillators. Elife. 2015 Oct 2;4. doi: 10.7554/eLife.09771.
pCI-BCD7-lacI-ssrA P62U18C50T16 v40-4a (496)plasmid for building 5n1lacI-ssrA Murray Rapid cell-free forward engineering of novel genetic ring oscillators. Elife. 2015 Oct 2;4. doi: 10.7554/eLife.09771.
pQacR-BCD7-tetR-ssrA P68U18C45T13 v40-5 (569)plasmid for building 5n2tetR-ssrA Murray Rapid cell-free forward engineering of novel genetic ring oscillators. Elife. 2015 Oct 2;4. doi: 10.7554/eLife.09771.
pTetR-BCD7-phlF-ssrA P66U18C51T15 v40-3a (492)plasmid for building 3n2, 5n1, 5n2phlF-ssrA Murray Rapid cell-free forward engineering of novel genetic ring oscillators. Elife. 2015 Oct 2;4. doi: 10.7554/eLife.09771.
pPhlF-BCD20-sfGFP-ssrA P64U23C39T13 v1-1 (462)1 color strong reporter used in studysfGFP-ssrA Murray Rapid cell-free forward engineering of novel genetic ring oscillators. Elife. 2015 Oct 2;4. doi: 10.7554/eLife.09771.
pPhlF-BCD22-sfGFP-ssrA P64U26C39T11 v1-1 (632)1 color weak reporter used in studysfGFP-ssrA Murray Rapid cell-free forward engineering of novel genetic ring oscillators. Elife. 2015 Oct 2;4. doi: 10.7554/eLife.09771.
pPhlF-BCD20-Citrine-ssrA P64U23C88T18 v1-1 (586)for building 3-color reporter plasmidCitrine-ssrA Murray Rapid cell-free forward engineering of novel genetic ring oscillators. Elife. 2015 Oct 2;4. doi: 10.7554/eLife.09771.
pSrpR-BCD20-Cerulean-ssrA P65U23C89T19 v50-2a (587)for building 3-color reporter plasmidCerulean-ssrA Murray Rapid cell-free forward engineering of novel genetic ring oscillators. Elife. 2015 Oct 2;4. doi: 10.7554/eLife.09771.
pTetR-BCD20-mCherry-ssrA P66U23C90T20 v40-3a (588)for building 3-color reporter plasmidmCherry-ssrA Murray Rapid cell-free forward engineering of novel genetic ring oscillators. Elife. 2015 Oct 2;4. doi: 10.7554/eLife.09771.
pJBL002Antisense Control PlasmidBacterial Expression, Synthetic Biology Lucks Creating small transcription activating RNAs. Nat Chem Biol. 2015 Mar;11(3):214-20. doi: 10.1038/nchembio.1737. Epub 2015 Feb 2.
pJBL2807AD1 STAR Antisense Expression PlasmidAD1 Antisense (Synthetic)Bacterial Expression, Synthetic Biology Lucks Creating small transcription activating RNAs. Nat Chem Biol. 2015 Mar;11(3):214-20. doi: 10.1038/nchembio.1737. Epub 2015 Feb 2.
pJBL2154pT181 STAR Antisense Expression PlasmidpT181 STAR Antisense (Synthetic)Synthetic Biology Lucks Creating small transcription activating RNAs. Nat Chem Biol. 2015 Mar;11(3):214-20. doi: 10.1038/nchembio.1737. Epub 2015 Feb 2.
pJBL2191pbuE STAR Antisense Expression PlasmidpbuE STAR Antisense (Synthetic)Bacterial Expression, Synthetic Biology Lucks Creating small transcription activating RNAs. Nat Chem Biol. 2015 Mar;11(3):214-20. doi: 10.1038/nchembio.1737. Epub 2015 Feb 2.
pJBL1865Anti-anti STAR Antisense Expression PlasmidAnti-anti STAR Antisense (Synthetic)Bacterial Expression, Synthetic Biology Lucks Creating small transcription activating RNAs. Nat Chem Biol. 2015 Mar;11(3):214-20. doi: 10.1038/nchembio.1737. Epub 2015 Feb 2.
pJBL2801AD1 Sense Expression PlasmidAD1 Sense (Synthetic)Bacterial Expression, Synthetic Biology Lucks Creating small transcription activating RNAs. Nat Chem Biol. 2015 Mar;11(3):214-20. doi: 10.1038/nchembio.1737. Epub 2015 Feb 2.
pJBL2148pT181 Sense Expression PlasmidpT181 Sense (Synthetic)Synthetic Biology Lucks Creating small transcription activating RNAs. Nat Chem Biol. 2015 Mar;11(3):214-20. doi: 10.1038/nchembio.1737. Epub 2015 Feb 2.
pJBL2184pbuE Sense Expression PlasmidpbuE SenseBacterial Expression, Synthetic Biology Lucks Creating small transcription activating RNAs. Nat Chem Biol. 2015 Mar;11(3):214-20. doi: 10.1038/nchembio.1737. Epub 2015 Feb 2.
pJBL1864Anti-anti Sense Expression PlasmidAnti-anti Sense (Synthetic)Bacterial Expression, Synthetic Biology Lucks Creating small transcription activating RNAs. Nat Chem Biol. 2015 Mar;11(3):214-20. doi: 10.1038/nchembio.1737. Epub 2015 Feb 2.
pJBL001Sense Control PlasmidBacterial Expression, Synthetic Biology Lucks Creating small transcription activating RNAs. Nat Chem Biol. 2015 Mar;11(3):214-20. doi: 10.1038/nchembio.1737. Epub 2015 Feb 2.
Reb_1 aka PAS619Expresses wild type R bodies from C. taeniospiralis in pETM11 backboneReb locus (Other)Bacterial Expression Silver A Tunable Protein Piston That Breaks Membranes to Release Encapsulated Cargo. ACS Synth Biol. 2016 Feb 3.
Reb_79 aka PAS620Reb_1 with RebA(Q91R)Reb locus (Other)Bacterial Expression Silver A Tunable Protein Piston That Breaks Membranes to Release Encapsulated Cargo. ACS Synth Biol. 2016 Feb 3.
Reb_80 aka PAS621Reb_1 with RebB(I67T, N84I)Reb locus (Other)Bacterial Expression Silver A Tunable Protein Piston That Breaks Membranes to Release Encapsulated Cargo. ACS Synth Biol. 2016 Feb 3.
Reb_81 aka PAS622Reb_1 with RebB(T28A, D72G)Reb locus (Other)Bacterial Expression Silver A Tunable Protein Piston That Breaks Membranes to Release Encapsulated Cargo. ACS Synth Biol. 2016 Feb 3.
Reb_82 aka PAS623Reb_1 with RebB(S82P, D88G)Reb locus (Other)Bacterial Expression Silver A Tunable Protein Piston That Breaks Membranes to Release Encapsulated Cargo. ACS Synth Biol. 2016 Feb 3.
Reb_83 aka PAS624Reb_1 with RebA(G53A, D89G)Reb locus (Other)Bacterial Expression Silver A Tunable Protein Piston That Breaks Membranes to Release Encapsulated Cargo. ACS Synth Biol. 2016 Feb 3.
Reb_140 aka PAS625Reb_1 with RebA(S88P)Reb locus (Other)Bacterial Expression Silver A Tunable Protein Piston That Breaks Membranes to Release Encapsulated Cargo. ACS Synth Biol. 2016 Feb 3.
Reb_65 aka PAS626Reb_1 with RebA(Y82A)Reb locus (Other)Bacterial Expression Silver A Tunable Protein Piston That Breaks Membranes to Release Encapsulated Cargo. ACS Synth Biol. 2016 Feb 3.
Reb_67 aka PAS627Reb_1 with RebA(S88A)Reb locus (Other)Bacterial Expression Silver A Tunable Protein Piston That Breaks Membranes to Release Encapsulated Cargo. ACS Synth Biol. 2016 Feb 3.
Reb_221 aka PAS628Reb_1 with RebA(G90A)Reb locus (Other)Bacterial Expression Silver A Tunable Protein Piston That Breaks Membranes to Release Encapsulated Cargo. ACS Synth Biol. 2016 Feb 3.
Reb_206 aka PAS629Expresses wild type R bodies with mNeonGreen-tagged second copy of RebB under a weak RBS downstream as well as soluble mCherryReb locus (Other)Bacterial Expression Silver A Tunable Protein Piston That Breaks Membranes to Release Encapsulated Cargo. ACS Synth Biol. 2016 Feb 3.
Reb_207 aka PAS630Reb_206 with RebA(S88P)Reb locus (Other)Bacterial Expression Silver A Tunable Protein Piston That Breaks Membranes to Release Encapsulated Cargo. ACS Synth Biol. 2016 Feb 3.
pETM6-HpaBCExpresses E. coli HpaBC in monocistronic form from ePathBrick vector, pETM6HpaB (Other), HpaC (Other)Bacterial Expression, Synthetic Biology Koffas "Optimization of naringenin and p-coumaric acid hydroxylation using the native E. coli hydroxylase complex, HpaBC". Biotechnol Prog. 2015 Oct 21. doi: 10.1002/btpr.2185.
pTRK669Helper plasmid for pORI plasmids. Chloramphenicol resistant, temperature sensitive replication in E. coli and gram positives. Encodes RepA.Bacterial Expression ; E. coli-gram positive bacteria shuttle vector Klaenhammer Efficient system for directed integration into the Lactobacillus acidophilus and Lactobacillus gasseri chromosomes via homologous recombination. Appl Environ Microbiol. 2001 Sep;67(9):4361-4.
pTKDP-neoDonor plasmid - kanamycin resistant donor cassetteBacterial Expression, Synthetic Biology Kuhlman An Integrated System for Precise Genome Modification in Escherichia coli. PLoS One. 2015 Sep 2;10(9):e0136963. doi: 10.1371/journal.pone.0136963. eCollection 2015.
pTKDP-catDonor plasmid - chloramphenicol resistant donor cassetteBacterial Expression, Synthetic Biology Kuhlman An Integrated System for Precise Genome Modification in Escherichia coli. PLoS One. 2015 Sep 2;10(9):e0136963. doi: 10.1371/journal.pone.0136963. eCollection 2015.
pTKDP-hphDonor plasmid - hygromycin resistant donor cassetteBacterial Expression, Synthetic Biology Kuhlman An Integrated System for Precise Genome Modification in Escherichia coli. PLoS One. 2015 Sep 2;10(9):e0136963. doi: 10.1371/journal.pone.0136963. eCollection 2015.
pTKDP-dhfrDonor plasmid - trimethoprim resistant donor cassetteBacterial Expression, Synthetic Biology Kuhlman An Integrated System for Precise Genome Modification in Escherichia coli. PLoS One. 2015 Sep 2;10(9):e0136963. doi: 10.1371/journal.pone.0136963. eCollection 2015.
pTKLP-tetAtetA landing pad template plasmidBacterial Expression, Synthetic Biology Kuhlman An Integrated System for Precise Genome Modification in Escherichia coli. PLoS One. 2015 Sep 2;10(9):e0136963. doi: 10.1371/journal.pone.0136963. eCollection 2015.
AEB-Theo27 pUC19This pUC19 vector contains a theophylline binding riboswitch, controlling the translation initiation rate of luciferase gene.Theo27 theophylline riboswitchSynthetic Biology Salis Automated physics-based design of synthetic riboswitches from diverse RNA aptamers. Nucleic Acids Res. 2015 Nov 30. pii: gkv1289.
pSS9Contains 600 bp homology arms to SS9 for one-step markerless genome integration in E. coligfpuv (Synthetic)Synthetic Biology Gill Rapid and Efficient One-Step Metabolic Pathway Integration in E. coli. ACS Synth Biol. 2016 Apr 22.
pBbE1k-3tyrosine hydroxylase from mouse, dihydropterine reductase from human, pterin-4a-carbinolamine dehydratase from humandihydropterine reductase (Homo sapiens), pterin-4a-carbinolamine dehydratase (Homo sapiens) Lee Engineering of L-tyrosine oxidation in Escherichia coli and microbial production of hydroxytyrosol. Metab Eng. 2012 Nov;14(6):603-10. doi: 10.1016/j.ymben.2012.08.002. Epub 2012 Aug 29.
pBbE1k-DDCdopa decarboxylase from pigdopa decarboxylase (Other) Lee Engineering of L-tyrosine oxidation in Escherichia coli and microbial production of hydroxytyrosol. Metab Eng. 2012 Nov;14(6):603-10. doi: 10.1016/j.ymben.2012.08.002. Epub 2012 Aug 29.
pG1AKModular shuttle vector for Geobacillus and E. coliBacterial Expression, Synthetic Biology ; Shuttle Vector Ellis The Geobacillus Plasmid Set: A Modular Toolkit for Thermophile Engineering. ACS Synth Biol. 2016 Jul 1.
pG1AK-mCherryModular shuttle vector for Geobacillus and E. colimCherryBacterial Expression, Synthetic Biology ; Shuttle Vector Ellis The Geobacillus Plasmid Set: A Modular Toolkit for Thermophile Engineering. ACS Synth Biol. 2016 Jul 1.
pG1AK-PheBModular shuttle vector for Geobacillus and E. coliPheB (Other)Bacterial Expression, Synthetic Biology ; Shuttle Vector Ellis The Geobacillus Plasmid Set: A Modular Toolkit for Thermophile Engineering. ACS Synth Biol. 2016 Jul 1.
pG1AK-sfGFPModular shuttle vector for Geobacillus and E. colisfGFPBacterial Expression, Synthetic Biology ; Shuttle Vector Ellis The Geobacillus Plasmid Set: A Modular Toolkit for Thermophile Engineering. ACS Synth Biol. 2016 Jul 1.
pG1CModular shuttle vector for Geobacillus and E. coliBacterial Expression, Synthetic Biology ; Shuttle Vector Ellis The Geobacillus Plasmid Set: A Modular Toolkit for Thermophile Engineering. ACS Synth Biol. 2016 Jul 1.
pG1KModular shuttle vector for Geobacillus and E. coliBacterial Expression, Synthetic Biology ; Shuttle Vector Ellis The Geobacillus Plasmid Set: A Modular Toolkit for Thermophile Engineering. ACS Synth Biol. 2016 Jul 1.
pG2KModular shuttle vector for Geobacillus and E. coliBacterial Expression, Synthetic Biology ; Shuttle Vector Ellis The Geobacillus Plasmid Set: A Modular Toolkit for Thermophile Engineering. ACS Synth Biol. 2016 Jul 1.
CY026Bacterial strain: E. coli BW25113 ΔlacI ΔaraC ΔsdiA Ptrc*-cinR Ptrc*-rhlRRhlR, CinRBacterial Expression, Synthetic Biology Bennett SYNTHETIC BIOLOGY. Emergent genetic oscillations in a synthetic microbial consortium. Science. 2015 Aug 28;349(6251):986-9. doi: 10.1126/science.aaa3794.
CY027Bacterial strain: E. coli BW25113 ΔlacI ΔaraC ΔsdiA Ptrc*-cinR Ptrc*-rhlRBacterial Expression, Synthetic Biology Bennett SYNTHETIC BIOLOGY. Emergent genetic oscillations in a synthetic microbial consortium. Science. 2015 Aug 28;349(6251):986-9. doi: 10.1126/science.aaa3794.
pTU1Backbone vector with internal BsaI removed by site-directed mutagenesisSynthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU1-A-lacZLevel 1 Destination vector - Position ALevel 1 - A site (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU1-B-lacZLevel 1 Destination vector - Position BLevel 1 - B site (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU1-C-lacZLevel 1 Destination vector - Position CLevel 1 - C site (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU1-D-lacZLevel 1 Destination vector - Position DLevel 1 - D site (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU1-A-RFPLevel 1 Destination vector - Position ALevel 1 - A site (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU1-B-RFPLevel 1 Destination vector - Position BLevel 1 - B site (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU1-C-RFPLevel 1 Destination vector - Position CLevel 1 - C site (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU1-D-RFPLevel 1 Destination vector - Position DLevel 1 - D site (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU1-D1-RFPLevel 1 Destination vector - Position D1Level 1 - D1 site (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU1-E-RFPLevel 1 Destination vector - Position ELevel 1 - E site (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU3-A-RFPLevel 3 Destination vector - Assembly of 2 Level 2 modules - ABLevel 3 - A site (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU3-B-RFPLevel 3 Destination vector - Assembly of 4 Level 2 modules - ABCDLevel 3 - B site (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBPBackbone vector with internal BsmBI removed by site-directed mutagenesisSynthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-lacZEntry vector for Bioparts - Oligo anneal or PCR with internal BsaI sites at 5' and 3' ends using NdeI/SphI cloningLevel 0 - Bioparts (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-ORFEntry vector for ORF's - PCR, gene synthesis or sub-clone using NdeI/BamHI. Alternatively use BsaI with CATA and TCGA fusion sites. Stop codon must be included upstream of chosen 3' restriction siteLevel 0 - ORFs (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU2-A-lacZLevel 2 Destination vector - Position A - Assembly of 4-5 TU's from Level 1-ABCD or ABCDELevel 2 - A site (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU2-B-lacZLevel 2 Destination vector - Position B - Assembly of 4-5 TU's from Level 1-ABCD or ABCDELevel 2 - B site (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU2-C-lacZLevel 2 Destination vector - Position C - Assembly of 4-5 TU's from Level 1-ABCD or ABCDELevel 2 - C site (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU2-D-lacZLevel 2 Destination vector - Position D - Assembly of 4-5 TU's from Level 1-ABCD or ABCDELevel 2 - D site (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU2-A-RFPLevel 2 Destination vector - Position A - Assembly of 4-5 TU's from Level 1-ABCD or ABCDELevel 2 - A site (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU2-B-RFPLevel 2 Destination vector - Position B - Assembly of 4-5 TU's from Level 1-ABCD or ABCDELevel 2 - B site (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU2-C-RFPLevel 2 Destination vector - Position C - Assembly of 4-5 TU's from Level 1-ABCD or ABCDELevel 2 - C site (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU2-D-RFPLevel 2 Destination vector - Position D - Assembly of 4-5 TU's from Level 1-ABCD or ABCDELevel 2 - D site (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU2-a-RFPLevel 2 Destination vector - Position A - Assembly of 2 TU's from Level 1-ABLevel 2 - a site (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU2-b-RFPLevel 2 Destination vector - Position B - Assembly of 3 TU's from Level 1-ABCLevel 2 - b site (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-ORF-eGFPLevel 0 Entry vector" ORF - eGFP "Enhanced green flourescent protein (eGFP) (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-ORF-eCFPLevel 0 Entry vector" ORF - eCFP"Enhanced cyan fluorescent protein (eCFP) (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-ORF-mCherryLevel 0 Entry vector" ORF - mCherry "mCherry fluorescent protein (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-J23100Level 0 - J23100 standard iGEM promoter (5' CTAT/3' GTAC fusion)J23100 promoter (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-J23108Level 0 - J23108 standard iGEM promoter (5' CTAT/3' GTAC fusion)J23108 promoter (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-J23114Level 0 - J23114 standard iGEM promoter (5' CTAT/3' GTAC fusion)J23114 promoter (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-SJM901Level 0 - SJM901 promoter (5' CTAT/3' GTAC fusion)SJM901 promoter (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-SJM902Level 0 - SJM902 promoter (5' CTAT/3' GTAC fusion)SJM902 promoter (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-SJM903Level 0 - SJM903 promoter (5' CTAT/3' GTAC fusion)SJM903 promoter (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-SJM905Level 0 - SJM905 promoter (5' CTAT/3' GTAC fusion)SJM905 promoter (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-SJM906Level 0 - SJM906 promoter (5' CTAT/3' GTAC fusion)SJM906 promoter (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-SJM908Level 0 - SJM908 promoter (5' CTAT/3' GTAC fusion)SJM908 promoter (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-SJM910Level 0 - SJM910 promoter (5' CTAT/3' GTAC fusion)SJM910 promoter (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-SJM911Level 0 - SJM911 promoter (5' CTAT/3' GTAC fusion)SJM911 promoter (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-SJM912Level 0 - SJM912 promoter (5' CTAT/3' GTAC fusion)SJM912 promoter (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-SJM914Level 0 - SJM914 promoter (5' CTAT/3' GTAC fusion)SJM914 promoter (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-SJM915Level 0 - SJM915 promoter (5' CTAT/3' GTAC fusion)SJM915 promoter (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-T7T7 promoter (5' CTAT/3' GTAC fusion)T7 promoter (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-T7_RBST7 promoter and RBS - derived from pET3a (5' CTAT/3' CATA) fusion)T7+RBS (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-T7_RBS-His6-ThrombinT7 promoter, RBS, His6-tag and thrombin cleave site - derived from pET15b (5' CTAT/3' CATA) fusion)T7+RBS+His6+thrombin (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP_BBa_B0034Bba_B0034 iGEM RBS (5' GTAC/3' CATA fusion)Bba_B0034 RBS (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-pET-RBSPET RBS - derived from pET vectors (5' GTAC/3' CATA fusion)pET RBS derived from pET vectors (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-Tag_linkerN-terminal linker with pET-RBS (5' GTAC/3' TAAA fusion)N-terminal linker with pET-RBS (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-HexHisHexahistidine N-terminal tag (5' TAAA/3' CATA fusion)Polyhistidine (HHHHHH) N-terminal tag (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-Strep(II)Streptavidin(II) N-terminal tag (5' TAAA/3' CATA fusion)Strep(II) N-terminal tag (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-TL1RBS_H1H1 RBS (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-TL2 RBS_H2H2 RBS (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-TL4RBS_H5H5 RBS (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-TL5RBS_M2M2 RBS (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-TL6RBS_M3M3 RBS (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-TL7RBS_M5M5 RBS (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-TL8RBS_L1L1 RBS (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-TL9RBS_L2L2 RBS (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-TL10RBS_L3L3 RBS (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-TL11RBS_L4L4 RBS (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-TL12RBS_L5L5 RBS (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-BBa_B0012BBa_B0012 iGEM terminator (5' TCGA/ 3' TGTT fusion)Bba_B0012 terminator (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-BBa_B0015BBa_B0015 iGEM terminator (5' TCGA/ 3' TGTT fusion)BBa_B0015 terminator (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-L3S2P21L3S2P21 terminatorL3S2P21 terminator (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-L3S1P51L3S1P51 terminatorL3S1P51 terminator (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-L3S1P32L3S1P32 terminatorL3S1P32 terminator (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-L3S1P11L3S1P11 terminatorL3S1P11 terminator (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-L2U8H11L2U8H11 terminatorL2U8H11 terminator (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-L2U5H11L2U5H11 terminatorL2U5H11 terminator (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-L2U5H08L2U5H08 terminatorL2U5H08 terminator (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-L2U3H03L2U3H03 terminatorL2U3H03 terminator (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-L2U2H09L2U2H09 terminatorL2U2H09 terminator (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-L1U1H09L1U1H09 terminatorL1U1H09 terminator (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-L1U1H08L1U1H08 terminatorL1U1H08 terminator (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU2_2aSecondary module (BpiI) site in pTU2a backboneSecondary module + Golden Gate pTU2a BsmBI destination site (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU2_2bSecondary module (BpiI) site in pTU2b backboneSecondary module + Golden Gate pTU2b BsmBI destination site (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pJK108Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2/synthetic)delta-12 fatty acid acetylenase (Other)Bacterial Expression Kappock Additional fatty acid desaturase and acetylenase constructs (unpublished)
pETM6-3F2-mCherryReporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3F2.Promoter 3F2 (orthogonal T7-lac variant) (Synthetic)Bacterial Expression, CRISPR, Synthetic Biology Koffas Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
pETM6-3H5-mCherryReporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3H5.Promoter 3H5 (orthogonal T7-lac variant) (Synthetic)Bacterial Expression, CRISPR, Synthetic Biology Koffas Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
pETM6-1B6-mCherryReporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1B6.Promoter 1B6 (orthogonal T7-lac variant) (Synthetic)Bacterial Expression, CRISPR, Synthetic Biology Koffas Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
pETM6-4F2-mCherryReporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4F2.Promoter 4F2 (orthogonal T7-lac variant) (Synthetic)Bacterial Expression, CRISPR, Synthetic Biology Koffas Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
pETM6-5F5-mCherryReporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 5F5.Promoter 5F5 (orthogonal T7-lac variant) (Synthetic)Bacterial Expression, CRISPR, Synthetic Biology Koffas Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
pETM6-1D4-mCherryReporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1D4.Promoter 1D4 (orthogonal T7-lac variant) (Synthetic)Bacterial Expression, CRISPR, Synthetic Biology Koffas Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
pETM6-4A6-mCherryReporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4A6.Promoter 4A6 (orthogonal T7-lac variant) (Synthetic)Bacterial Expression, CRISPR, Synthetic Biology Koffas Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
pETM6-3A2-mCherryReporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3A2.Promoter 3A2 (orthogonal T7-lac variant) (Synthetic)Bacterial Expression, CRISPR, Synthetic Biology Koffas Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
pETM6-1E4-mCherryReporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1E4.Promoter 1E4 (orthogonal T7-lac variant) (Synthetic)Bacterial Expression, CRISPR, Synthetic Biology Koffas Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
pdCas9-M-C4CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant C4.Repressor C4 (orthogonal T7-lac repressor) (Synthetic)Bacterial Expression, CRISPR, Synthetic Biology Koffas Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
pdCas9-M-3F2CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3F2.Repressor C4 (orthogonal T7-lac repressor) (Synthetic)Bacterial Expression, CRISPR, Synthetic Biology Koffas Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
pdCas9-M-3H5CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3H5.Repressor 3H5 (orthogonal T7-lac repressor) (Synthetic)Bacterial Expression, CRISPR, Synthetic Biology Koffas Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
pdCas9-M-1B6CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 1B6.Repressor 1B6 (orthogonal T7-lac repressor) (Synthetic)Bacterial Expression, CRISPR, Synthetic Biology Koffas Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
pdCas9-M-4F2CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 4F2.Repressor 4F2 (orthogonal T7-lac repressor) (Synthetic)Bacterial Expression, CRISPR, Synthetic Biology Koffas Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
pdCas9-M-5F5CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 5F5.Repressor 5F5 (orthogonal T7-lac repressor) (Synthetic)Bacterial Expression, CRISPR, Synthetic Biology Koffas Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
pdCas9-M-1D4CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 1D4.Repressor 1D4 (orthogonal T7-lac repressor) (Synthetic)Bacterial Expression, CRISPR, Synthetic Biology Koffas Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
pdCas9-M-4A6CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 4A6.Repressor 4A6 (orthogonal T7-lac repressor) (Synthetic)Bacterial Expression, CRISPR, Synthetic Biology Koffas Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
pdCas9-M-3A2CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3A2.Repressor 3A2 (orthogonal T7-lac repressor) (Synthetic)Bacterial Expression, CRISPR, Synthetic Biology Koffas Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
pdCas9-M-1E4CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 1E4.Repressor 1E4 (orthogonal T7-lac repressor) (Synthetic)Bacterial Expression, CRISPR, Synthetic Biology Koffas Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
pdCas9-M-G6CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant G6.Repressor G6 (orthogonal T7-lac repressor) (Synthetic)Bacterial Expression, CRISPR, Synthetic Biology Koffas Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
pETM6-G6-vioABEProdeoxyviolacein pathway (vioABE) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variant G6.PG6-vioABE (Other)Bacterial Expression, CRISPR, Synthetic Biology Koffas Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
pETM6-G6-vioABE-4A6-vioCDeoxyviolacein pathway (vioABEC) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE) and 4A6 (vioC) for orthogonal flux redirection.PG6-vioABE-P4A6-vioC (Other)Bacterial Expression, CRISPR, Synthetic Biology Koffas Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
pETM6-G6-vioABE-4A6-vioC-3A2-vioDViolacein pathway (vioABECD) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE), 4A6 (vioC), and 3A2 (vioD) for orthogonal flux redirection.PG6-vioABE-P4A6-vioC-P3A2-vioD (Other)Bacterial Expression, CRISPR, Synthetic Biology Koffas Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
pEvol-pAcFRS.2.t1tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAGpAcFRS.2.t1 (Synthetic), pAcFRS.2.t1 (Synthetic)Bacterial Expression Isaacs Evolution of translation machinery in recoded bacteria enables multi-site incorporation of nonstandard amino acids. Nat Biotechnol. 2015 Dec;33(12):1272-1279. doi: 10.1038/nbt.3372. Epub 2015 Nov 16.
pEvol-pAcFRS.1.t1tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAGpAcFRS.1.t1 (Synthetic), pAcFRS.1.t1 (Synthetic)Bacterial Expression Isaacs Evolution of translation machinery in recoded bacteria enables multi-site incorporation of nonstandard amino acids. Nat Biotechnol. 2015 Dec;33(12):1272-1279. doi: 10.1038/nbt.3372. Epub 2015 Nov 16.
pEvol-pAzFRS.2.t1tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAGpAzFRS.2.t1 (Synthetic), pAzFRS.2.t1 (Synthetic)Bacterial Expression Isaacs Evolution of translation machinery in recoded bacteria enables multi-site incorporation of nonstandard amino acids. Nat Biotechnol. 2015 Dec;33(12):1272-1279. doi: 10.1038/nbt.3372. Epub 2015 Nov 16.
pEvol-pAzFRS.1.t1tRNA synthetase/tRNA pair for the in vivo incorporation of p-azido-l-phenylalanine, into proteins in E coli in response to the amber codon, TAGpAzFRS.1.t1 (Synthetic), pAzFRS.1.t1Bacterial Expression Isaacs Evolution of translation machinery in recoded bacteria enables multi-site incorporation of nonstandard amino acids. Nat Biotechnol. 2015 Dec;33(12):1272-1279. doi: 10.1038/nbt.3372. Epub 2015 Nov 16.
C321.deltaA (Isaacs lab)Genomically recoded organism (E. coli C321.A, CP006698.1, GI:54981157). This strain is different than Addgene #68306 as it still retains the serB genenoneBacterial Expression Isaacs Evolution of translation machinery in recoded bacteria enables multi-site incorporation of nonstandard amino acids. Nat Biotechnol. 2015 Dec;33(12):1272-1279. doi: 10.1038/nbt.3372. Epub 2015 Nov 16.
pJKR-H-benMMuconate biosensor. Expresses GFP proportional to muconate concentration within the cell.muconate biosensing apparatus Church Genetically encoded sensors enable real-time observation of metabolite production. Proc Natl Acad Sci U S A. 2016 Feb 8. pii: 201600375.
pZ8-T_dCas9pZ8-1 plasmid carrying dcas9, driven by the IPTG-inducible Ptac promoter, KanRdcas9Bacterial Expression, CRISPR, Synthetic Biology Lu Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi). ACS Synth Biol. 2016 Feb 16.
pZ8-P_dCas9pZ8-1 plasmid carrying dcas9 driven by the propionate-inducible prpD2 promoter (PprpD2), KanRdcas9Bacterial Expression, CRISPR, Synthetic Biology Lu Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi). ACS Synth Biol. 2016 Feb 16.
pZ8-PtacIPTG inducible version of pZ8-1 plasmid, to which lacIq was added, KanRBacterial Expression, Synthetic Biology Lu Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi). ACS Synth Biol. 2016 Feb 16.
pZ8-PrppZ8-1 with the PprpD2 propionate-inducible promoter instead of Ptac, KanRPropionate inducible promoterBacterial Expression, Synthetic Biology Lu Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi). ACS Synth Biol. 2016 Feb 16.
pAL-pgi_TpAL374 plasmid carrying the pgi (T) sgRNA, targeting the template strand of pgi, SpecRsgRNA pgi (T)Bacterial Expression, CRISPR, Synthetic Biology Lu Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi). ACS Synth Biol. 2016 Feb 16.
pAL-pgi_NTpAL374 plasmid carrying the pgi (NT) sgRNA, targeting the non-template strand of pgi, SpecRsgRNA pgi (NT) (Synthetic)Bacterial Expression, CRISPR, Synthetic Biology Lu Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi). ACS Synth Biol. 2016 Feb 16.
pAL-pck_TpAL374 plasmid carrying the pck (T) sgRNA targeting, the template strand of pck, SpecRsgRNA pck (T) (Synthetic)Bacterial Expression, CRISPR, Synthetic Biology Lu Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi). ACS Synth Biol. 2016 Feb 16.
pAL-pck_NTpAL374 plasmid carrying the pck (NT) sgRNA, targeting the non-template strand of pck, SpecRsgRNA pck (NT) (Synthetic)Bacterial Expression, CRISPR, Synthetic Biology Lu Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi). ACS Synth Biol. 2016 Feb 16.
pAL-pyk_TpAL374 plasmid carrying the pyk (T) sgRNA targeting the template strand of pyk, SpecRsgRNA pyk (T) (Synthetic)Bacterial Expression, CRISPR, Synthetic Biology Lu Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi). ACS Synth Biol. 2016 Feb 16.
pAL-pyk_NTpAL374 plasmid carrying the pyk (NT) sgRNA targeting the non-template strand of pyk, SpecRsgRNA pyk (NT) (Synthetic)Bacterial Expression, CRISPR Lu Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi). ACS Synth Biol. 2016 Feb 16.
pRIM3-rfpGentR version of pRIM2 carrying rfprfpBacterial Expression, CRISPR, Synthetic Biology Lu Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi). ACS Synth Biol. 2016 Feb 16.
pAL-rfp_TpAL374 plasmid carrying the rfp (T) sgRNA targeting the template strand of rfp, SpecRsgRNA rfp (T) (Synthetic)Bacterial Expression, Synthetic Biology Lu Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi). ACS Synth Biol. 2016 Feb 16.
pTU2S-a (p15A origin)Secondary module (BpiI) site in pTU2-a backbone with p15A (low-copy) origin of replicationGolden Gate destination vector - Level 2, 2 TU's with secondary module and p15A origin (Synthetic)Bacterial Expression, Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU2S-b (p15A origin)Secondary module (BpiI) site in pTU2-a backbone with p15A (low-copy) origin of replicationGolden Gate destination vector - Level 2, 3 TU's with secondary module and p15A origin (Synthetic)Bacterial Expression, Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU2-A-RFP (p15A origin)pTU2-A backbone, with RFP negative selection and p15A (low-copy) origin of replicationGolden Gate destination vector - Level 2, 4 TU's with RFP and p15A origin (Synthetic)Bacterial Expression, Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU2S-a (colE1 origin)Secondary module (BpiI) site in pTU2-a backbone with colE1 (medium-copy) origin of replicationGolden Gate destination vector - Level 2, 2 TU's with secondary module and colE1 origin (Synthetic)Bacterial Expression, Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU2S-b (colE1 origin)Secondary module (BpiI) site in pTU2-a backbone with colE1 (medium-copy) origin of replicationGolden Gate destination vector - Level 2, 3 TU's with secondary module and colE1 origin (Synthetic)Bacterial Expression, Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTU2-A-RFP (colE1 origin)pTU2-A backbone, with RFP negative selection and colE1 (medium-copy) origin of replicationGolden Gate destination vector - Level 2, 4 TU's with RFP and colE1 origin of replication (Synthetic)Bacterial Expression, Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-Bba_K822000L-Lactate inducible promoter from E. coli MG1655Bba_K822000 (L-Lactate promoter - E. coli MG1655) (Other)Bacterial Expression, Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-lldRL-lactate repressor (LldR) from E. coli MG1655lldR (Other)Bacterial Expression, Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-T7RNAPT7 RNA polymerase PCR cloned from BL21 (DE3) genomic DNAT7 RNAP (Other)Bacterial Expression, Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-BBa_J23100BBa_J23100 promoter (iGEM collection)Bba_J23100 constitutive promoter (Sigma70) (Synthetic)Bacterial Expression, Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pBP-TL9TL9 RBS (AAAAAG)TL9 RBS (Synthetic)Bacterial Expression, Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pCIRCNpuVector for SICLOPPS-mediated protein circularization using the Nostoc punctiforme DnaE intein.Bacterial Expression, Synthetic Biology Eils Backbone circularization of Bacillus subtilis family 11 xylanase increases its thermostability and its resistance against aggregation. Mol Biosyst. 2015 Nov 10;11(12):3231-43. doi: 10.1039/c5mb00341e.
B0030m_BCMoClo Basic Part: RBS - Weiss RBS, high strength. Modified from Bba_B0030 to adjust spacing in MC system. [B:B0030m:C]RBS (Synthetic)Synthetic Biology Densmore B0030m_BC (unpublished)
pSG4K5For cloning and constitutive expression of sgRNAs in E.coli. New sgRNAs are cloned into SapI sites and multiplex sgRNAs are cloned into BsaI sites. Kanamycin resistant with pSC101 origin of rep.sgRNA (Synthetic)Bacterial Expression, CRISPR, Synthetic Biology Wang Targeted Large-Scale Deletion of Bacterial Genomes Using CRISPR-Nickases. ACS Synth Biol. 2015 Nov 20;4(11):1217-25. doi: 10.1021/acssynbio.5b00132. Epub 2015 Oct 25.
pCas9(D10A)Nicking Cas9 derived from pWT Cas9 (Addgene #44250). Ampicillin resistance marker, the tetracycline repressor (TetR), a ColE1 origin of replication and Streptococcus pyogenes Cas9 D10ACas9 D10A (Synthetic)Bacterial Expression, Synthetic Biology Wang Targeted Large-Scale Deletion of Bacterial Genomes Using CRISPR-Nickases. ACS Synth Biol. 2015 Nov 20;4(11):1217-25. doi: 10.1021/acssynbio.5b00132. Epub 2015 Oct 25.
pAmeR-F1AmeR-F1 gatePTac-RBSF1-AmeR-PAmeR-YFP (Other)Bacterial Expression Voigt Genetic circuit design automation. Science. 2016 Apr 1;352(6281):aac7341. doi: 10.1126/science.aac7341.
pAmtR-A1AmtR-A1 gatePTac-RBSA1-AmtR-PAmtR-YFP (Other)Bacterial Expression Voigt Genetic circuit design automation. Science. 2016 Apr 1;352(6281):aac7341. doi: 10.1126/science.aac7341.
pBM3R1-B1BM3R1-B1 gatePTac-RBSB1-BM3R1-PBM3R1-YFP (Other)Bacterial Expression Voigt Genetic circuit design automation. Science. 2016 Apr 1;352(6281):aac7341. doi: 10.1126/science.aac7341.
pBM3R1-B2BM3R1-B2 gatePTac-RBSB2-BM3R1-PBM3R1-YFP (Other)Bacterial Expression Voigt Genetic circuit design automation. Science. 2016 Apr 1;352(6281):aac7341. doi: 10.1126/science.aac7341.
pBM3R1-B3BM3R1-B3 gatePTac-RBSB3-BM3R1-PBM3R1-YFP (Other)Bacterial Expression Voigt Genetic circuit design automation. Science. 2016 Apr 1;352(6281):aac7341. doi: 10.1126/science.aac7341.
pHlyIIR-H1HlyIIR-H1 gatePTac-RBSH1-HlyIIR-PHlyIIR-YFP (Other)Bacterial Expression Voigt Genetic circuit design automation. Science. 2016 Apr 1;352(6281):aac7341. doi: 10.1126/science.aac7341.
pIcaRA-I1IcaRA-I1 gatePTac-RBSI1-IcaRA-PIcaR-YFP (Other)Bacterial Expression Voigt Genetic circuit design automation. Science. 2016 Apr 1;352(6281):aac7341. doi: 10.1126/science.aac7341.
pLitR-L1LitR-L1 gatePTac-RBSL1-LitR-PLitR-YFP (Other)Bacterial Expression Voigt Genetic circuit design automation. Science. 2016 Apr 1;352(6281):aac7341. doi: 10.1126/science.aac7341.
pLmrA-N1LmrA-N1 gatePTac-RBSN1-LmrA-PLmrA-YFP (Other)Bacterial Expression Voigt Genetic circuit design automation. Science. 2016 Apr 1;352(6281):aac7341. doi: 10.1126/science.aac7341.
pPhlF-P1PhlF-P1 gatePTac-RBSP1-PhlF-PPhlF-YFP (Other)Bacterial Expression Voigt Genetic circuit design automation. Science. 2016 Apr 1;352(6281):aac7341. doi: 10.1126/science.aac7341.
pPhlF-P3PhlF-P3 gatePTac-RBSP3-PhlF-PPhlF-YFP (Other)Bacterial Expression Voigt Genetic circuit design automation. Science. 2016 Apr 1;352(6281):aac7341. doi: 10.1126/science.aac7341.
pQacR-Q1QacR-Q1 gatePTac-RBSQ1-QacR-PQacR-YFP (Other)Bacterial Expression Voigt Genetic circuit design automation. Science. 2016 Apr 1;352(6281):aac7341. doi: 10.1126/science.aac7341.
pQacR-Q2QacR-Q2 gatePTac-RBSQ2-QacR-PQacR-YFP (Other)Bacterial Expression Voigt Genetic circuit design automation. Science. 2016 Apr 1;352(6281):aac7341. doi: 10.1126/science.aac7341.
pSrpR-S1SrpR-S1 gatePTac-RBSS1-SrpR-PSrpR-YFP (Other)Bacterial Expression Voigt Genetic circuit design automation. Science. 2016 Apr 1;352(6281):aac7341. doi: 10.1126/science.aac7341.
pSrpR-S2SrpR-S2 gatePTac-RBSS2-SrpR-PSrpR-YFP (Other)Bacterial Expression Voigt Genetic circuit design automation. Science. 2016 Apr 1;352(6281):aac7341. doi: 10.1126/science.aac7341.
pSrpR-S3SrpR-S3 gatePTac-RBSS3-SrpR-PSrpR-YFP (Other)Bacterial Expression Voigt Genetic circuit design automation. Science. 2016 Apr 1;352(6281):aac7341. doi: 10.1126/science.aac7341.
pSrpR-S4SrpR-S4 gatePTac-RBSS4-SrpR-PSrpR-YFP (Other)Bacterial Expression Voigt Genetic circuit design automation. Science. 2016 Apr 1;352(6281):aac7341. doi: 10.1126/science.aac7341.
pAN1201Circuit backbone plasmid (LacZa insert)Bacterial Expression Voigt Genetic circuit design automation. Science. 2016 Apr 1;352(6281):aac7341. doi: 10.1126/science.aac7341.
pAN3938Circuit plasmid: 0xF6Circuit 0xF6 (Other)Bacterial Expression Voigt Genetic circuit design automation. Science. 2016 Apr 1;352(6281):aac7341. doi: 10.1126/science.aac7341.
pAN4036Output plasmid: PPhlF-PAmtR-YFPPPhlF-PBetI-YFP (Other)Bacterial Expression Voigt Genetic circuit design automation. Science. 2016 Apr 1;352(6281):aac7341. doi: 10.1126/science.aac7341.
pAN3945Circuit plasmid: 0x41Circuit 0x41 (Other)Bacterial Expression Voigt Genetic circuit design automation. Science. 2016 Apr 1;352(6281):aac7341. doi: 10.1126/science.aac7341.
pAN4023Output plasmid: PBM3R1-YFPPBM3R1-YFP (Other)Bacterial Expression Voigt Genetic circuit design automation. Science. 2016 Apr 1;352(6281):aac7341. doi: 10.1126/science.aac7341.
pAN3944Circuit plasmid: 0x1CCircuit 0x1C (Other)Bacterial Expression Voigt Genetic circuit design automation. Science. 2016 Apr 1;352(6281):aac7341. doi: 10.1126/science.aac7341.
pAN4037Output plasmid: PPhlF-PBetI-YFPPPhlF-PBetI-YFP (Other)Bacterial Expression Voigt Genetic circuit design automation. Science. 2016 Apr 1;352(6281):aac7341. doi: 10.1126/science.aac7341.
pJM100: no promoter RFP on pCM66TpCM66T RFP control vector: no promoter driving maxRFP (BBa_E1010)RFP (BioBrick part number BBa_E1010) (Synthetic)Bacterial Expression, Synthetic Biology Lidstrom broad host range pcm66t (unpublished)
pJM102: pHps2 RFP on pCM66TpCM66T with promoter pHps2 driving RFP expression (maxRFP = BBa_E1010)RFP (BioBrick part number BBa_E1010) (Synthetic)Bacterial Expression, Synthetic Biology Lidstrom broad host range pcm66t (unpublished)
pJM103: 150bp Hps2 RFP on pCM66TpCM66T with shortened pHps2 promoter driving RFP expression (maxRFP = BBa_E1010)RFP (BioBrick part number BBa_E1010) (Synthetic)Bacterial Expression, Synthetic Biology Lidstrom broad host range pcm66t (unpublished)
pJM104: pTrc RFP on pCM66TpCM66T with promoter pTrc driving RFP expression (maxRFP = BBa_E1010)RFP (BioBrick part number BBa_E1010) Lidstrom broad host range pcm66t (unpublished)
pJM105: pTac RFP on pCM66TpCM66T with promoter pTac driving RFP expression (maxRFP = BBa_E1010)RFP (BioBrick part number BBa_E1010)Bacterial Expression, Synthetic Biology Lidstrom broad host range pcm66t (unpublished)
ZIKV_Sensor_27B_LacZToehold switch sensor to detect Zika VirusZIKV_sensor_27B_LacZ (Synthetic)Bacterial Expression Collins Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components. Cell. 2016 May 6. pii: S0092-8674(16)30505-0. doi: 10.1016/j.cell.2016.04.059.
ZIKV_Sensor_32B_LacZToehold switch sensor to detect Zika VirusZIKV_sensor_32B_LacZ (Synthetic)Bacterial Expression Collins Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components. Cell. 2016 May 6. pii: S0092-8674(16)30505-0. doi: 10.1016/j.cell.2016.04.059.
ZIKV_Trigger_27BPortion of Zika Virus genome (KU312312: 1348-1494) containing sensor 27B trigger sequenceZIKV_Trigger_27B (Synthetic)Bacterial Expression Collins Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components. Cell. 2016 May 6. pii: S0092-8674(16)30505-0. doi: 10.1016/j.cell.2016.04.059.
ZIKV_Trigger_32BPortion of Zika Virus genome (KU312312: 7166-7299) containing sensor 32B trigger sequenceZIKV_Trigger_32B (Synthetic)Bacterial Expression Collins Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components. Cell. 2016 May 6. pii: S0092-8674(16)30505-0. doi: 10.1016/j.cell.2016.04.059.
ZIKV_NASBA_27BPortion of Zika Virus genome (KU312312: 1248-1594) containing sensor 27B trigger sequence and NASBA amplification sitesZIKV_NASBA_27B (Synthetic)Bacterial Expression Collins Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components. Cell. 2016 May 6. pii: S0092-8674(16)30505-0. doi: 10.1016/j.cell.2016.04.059.
ZIKV_NASBA_32BPortion of Zika Virus genome (KU312312: 7066-7399) containing sensor 32B trigger sequence and NASBA amplification sitesZIKV_NASBA_32B (Synthetic)Bacterial Expression Collins Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components. Cell. 2016 May 6. pii: S0092-8674(16)30505-0. doi: 10.1016/j.cell.2016.04.059.
pZA16mflonPlasmid for arabinose-induced expression of mf-lon. Note that this plasmid contains the original mf-lon cassette that is not codon optimized for expression in E. coli.mf-lon (Other)Bacterial Expression Collins Tunable protein degradation in bacteria. Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub 2014 Nov 17.
pECGMC3Plasmid for mf-lon mediated mCherry degradation in Lactococcus lactis. Contains mcherry-pdt3mf-lon (Other)Bacterial Expression Collins Tunable protein degradation in bacteria. Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub 2014 Nov 17.
pECM1Plasmid that serves as the PCR template to create the genomic pdt1 insertions contained in the EPD library. Plasmid contains the R6K origin of replication so needs pir gene to replicatepdt1 (Other)Bacterial Expression Collins Tunable protein degradation in bacteria. Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub 2014 Nov 17.
pECT1Plasmid that serves as the PCR template to create genomic pdt1 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicatepdt1 (Other)Bacterial Expression Collins Tunable protein degradation in bacteria. Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub 2014 Nov 17.
pECT1BPlasmid that serves as the PCR template to create genomic pdt1B insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicatepdt1B (Other)Bacterial Expression Collins Tunable protein degradation in bacteria. Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub 2014 Nov 17.
pECT1DPlasmid that serves as the PCR template to create genomic pdt1D insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicatepdt1D (Other)Bacterial Expression Collins Tunable protein degradation in bacteria. Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub 2014 Nov 17.
pECT1EPlasmid that serves as the PCR template to create genomic pdt1E insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicatepdt1E (Other)Bacterial Expression Collins Tunable protein degradation in bacteria. Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub 2014 Nov 17.
pECT3Plasmid that serves as the PCR template to create genomic pdt3 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicatepdt3 (Other)Bacterial Expression Collins Tunable protein degradation in bacteria. Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub 2014 Nov 17.
pECT5Plasmid that serves as the PCR template to create genomic pdt5 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicatepdt5 (Other)Bacterial Expression Collins Tunable protein degradation in bacteria. Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub 2014 Nov 17.
pZE27GFPPlasmid for constitutive expression of gfpgfp (Other)Bacterial Expression Collins Tunable protein degradation in bacteria. Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub 2014 Nov 17.
pZE27GFP0Plasmid for constitutive expression of gfp-pdtgfp-pdt (Other)Bacterial Expression Collins Tunable protein degradation in bacteria. Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub 2014 Nov 17.
pZE27GFP1Plasmid for constitutive expression of gfp-pdt1gfp-pdt#1 (Other)Bacterial Expression Collins Tunable protein degradation in bacteria. Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub 2014 Nov 17.
pZE27GFP2Plasmid for constitutive expression of gfp-pdt2gfp-pdt#2 (Other)Bacterial Expression Collins Tunable protein degradation in bacteria. Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub 2014 Nov 17.
pZE27GFP3Plasmid for constitutive expression of gfp-pdt3gfp-pdt#3 (Other)Bacterial Expression Collins Tunable protein degradation in bacteria. Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub 2014 Nov 17.
pZE27GFP4Plasmid for constitutive expression of gfp-pdt4gfp-pdt#4 (Other)Bacterial Expression Collins Tunable protein degradation in bacteria. Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub 2014 Nov 17.
pZE27GFP5Plasmid for constitutive expression of gfp-pdt5gfp-pdt#5 (Other)Bacterial Expression Collins Tunable protein degradation in bacteria. Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub 2014 Nov 17.
pZE27GFP3APlasmid for constitutive expression of gfp-pdt3Agfp-pdt#3A (Other)Bacterial Expression Collins Tunable protein degradation in bacteria. Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub 2014 Nov 17.
pZE27GFP3BPlasmid for constitutive expression of gfp-pdt3Bgfp-pdt#3C (Other)Bacterial Expression Collins Tunable protein degradation in bacteria. Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub 2014 Nov 17.
pZE27GFP3CPlasmid for constitutive expression of gfp-pdt3Cgfp-pdt#3C (Other)Bacterial Expression Collins Tunable protein degradation in bacteria. Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub 2014 Nov 17.
pZE27GFP3DPlasmid for constitutive expression of gfp-pdt3Dgfp-pdt#3D (Other)Bacterial Expression Collins Tunable protein degradation in bacteria. Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub 2014 Nov 17.
pZE27GFP3EPlasmid for constitutive expression of gfp-pdt3Egfp-pdt#3E (Other)Bacterial Expression Collins Tunable protein degradation in bacteria. Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub 2014 Nov 17.
pZE27MC3Plasmid for constitutive expression of mcherry-pdt3mcherry-pdt#3 (Other)Bacterial Expression Collins Tunable protein degradation in bacteria. Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub 2014 Nov 17.
pECJ3Toggle switch plasmid containing lacI-pdt3lacI-pdt3 (Other)Bacterial Expression Collins Tunable protein degradation in bacteria. Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub 2014 Nov 17.
pECJ3BToggle switch plasmid containing lacI-pdt3BlacI-pdt3B (Other)Bacterial Expression Collins Tunable protein degradation in bacteria. Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub 2014 Nov 17.
pECJ3DToggle switch plasmid containing lacI-pdt3DlacI-pdt3D (Other)Bacterial Expression Collins Tunable protein degradation in bacteria. Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub 2014 Nov 17.
pET22b_PstS_1Expression of single-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensorpstS (A197C) (Other)Bacterial Expression Webb Development of a Reagentless Biosensor for Inorganic Phosphate, Applicable over a Wide Concentration Range. Biochemistry. 2015 Aug 18;54(32):5054-62. doi: 10.1021/acs.biochem.5b00449. Epub 2015 Aug 4.
pET22b_PstS_2Expression of double-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensorpstS (A17C,A197C) (Other)Bacterial Expression Webb Development of a Reagentless Biosensor for Inorganic Phosphate, Applicable over a Wide Concentration Range. Biochemistry. 2015 Aug 18;54(32):5054-62. doi: 10.1021/acs.biochem.5b00449. Epub 2015 Aug 4.
pHis17_ParM _1 Expression of single-cysteine variant of plasmid segregation protein, ParM, as scaffold for ADP biosensorparM (K33A I27C T174A T175N C287A) (Other)Bacterial Expression Webb A biosensor for fluorescent determination of ADP with high time resolution. J Biol Chem. 2009 Nov 27;284(48):33130-8. doi: 10.1074/jbc.M109.047118. Epub 2009 Sep 29.
pET22b_EcSSB_1Expression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensorssb (G26C) (Other)Bacterial Expression Webb Fluorescent single-stranded DNA binding protein as a probe for sensitive, real-time assays of helicase activity. Biophys J. 2008 Oct;95(7):3330-9. doi: 10.1529/biophysj.108.133512. Epub 2008 Jul 3.
pET22b_EcSSB_2Expression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensorssb (W88C) (Other)Bacterial Expression Webb Visualizing helicases unwinding DNA at the single molecule level. Nucleic Acids Res. 2010 Jul;38(13):4448-57. doi: 10.1093/nar/gkq173. Epub 2010 Mar 28.
pTEV5_ RpMatB_1Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensormatB (His6 C106A R286C Q457C K488A) (Other)Bacterial Expression Webb A Fluorescent, Reagentless Biosensor for ATP, Based on Malonyl-Coenzyme A Synthetase. ACS Chem Biol. 2015 Nov 20;10(11):2650-7. doi: 10.1021/acschembio.5b00346. Epub 2015 Sep 21.
pTEV5_ RpMatB_2Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - tighter bindingmatB (His6 C106A S170A R286C Q457C K488A) (Other)Bacterial Expression Webb Development of a range of fluorescent reagentless biosensors for ATP, based on malonyl-coenzyme A synthetase. PLoS One. 2017 Jun 21;12(6):e0179547. doi: 10.1371/journal.pone.0179547. eCollection 2017.
pTEV5_ RpMatB_3Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - very weak bindingmatB (His6 C106A T167A R286C Q457C K488A) (Other)Bacterial Expression Webb Development of a range of fluorescent reagentless biosensors for ATP, based on malonyl-coenzyme A synthetase. PLoS One. 2017 Jun 21;12(6):e0179547. doi: 10.1371/journal.pone.0179547. eCollection 2017.
pTEV5_ RpMatB_4Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - weak bindingmatB (His6 C106A R286C T303A Q457C K488A) (Other)Bacterial Expression Webb Development of a range of fluorescent reagentless biosensors for ATP, based on malonyl-coenzyme A synthetase. PLoS One. 2017 Jun 21;12(6):e0179547. doi: 10.1371/journal.pone.0179547. eCollection 2017.
pZS2oxySp*-RBS30-Bxbi-proD-oxyRSynthetic gene circuit synthetic circuit (Other) Lu Synthetic mixed-signal computation in living cells. Nat Commun. 2016 Jun 3;7:11658. doi: 10.1038/ncomms11658.
Bxbi GFP BAC ReporterSynthetic gene circuitsynthetic circuit (Other) Lu Synthetic mixed-signal computation in living cells. Nat Commun. 2016 Jun 3;7:11658. doi: 10.1038/ncomms11658.
pZS2katGp-RBS31-PhiC31-proD-oxyRSynthetic gene circuitsynthetic circuit (Other) Lu Synthetic mixed-signal computation in living cells. Nat Commun. 2016 Jun 3;7:11658. doi: 10.1038/ncomms11658.
PhiC31 GFP BAC ReporterSynthetic gene circuitsynthetic circuit (Other) Lu Synthetic mixed-signal computation in living cells. Nat Commun. 2016 Jun 3;7:11658. doi: 10.1038/ncomms11658.
pZS2katGp-RBS33-TP901-proD-oxyRsynthetic gene circuitsynthetic circuit (Other) Lu Synthetic mixed-signal computation in living cells. Nat Commun. 2016 Jun 3;7:11658. doi: 10.1038/ncomms11658.
TP901 GFP BAC Reportersynthetic gene circuitsynthetic circuit (Other) Lu Synthetic mixed-signal computation in living cells. Nat Commun. 2016 Jun 3;7:11658. doi: 10.1038/ncomms11658.
pZS1katGp-RBS31-PhiC31-proD-oxyRsynthetic gene circuitsynthetic circuit (Other) Lu Synthetic mixed-signal computation in living cells. Nat Commun. 2016 Jun 3;7:11658. doi: 10.1038/ncomms11658.
Bxbi+PhiC31 GFP Bandpass BAC Reportersynthetic gene circuitsynthetic circuit (Other) Lu Synthetic mixed-signal computation in living cells. Nat Commun. 2016 Jun 3;7:11658. doi: 10.1038/ncomms11658.
pZS1katGp-RBS33-TP901-proD-oxyRsynthetic gene circuitsynthetic gene circuit (Other) Lu Synthetic mixed-signal computation in living cells. Nat Commun. 2016 Jun 3;7:11658. doi: 10.1038/ncomms11658.
Ternary BAC Reportersynthetic gene circuitsynthetic gene circuit (Other) Lu Synthetic mixed-signal computation in living cells. Nat Commun. 2016 Jun 3;7:11658. doi: 10.1038/ncomms11658.
ADC BAC Reportersynthetic gene circuitsynthetic gene circuit (Other) Lu Synthetic mixed-signal computation in living cells. Nat Commun. 2016 Jun 3;7:11658. doi: 10.1038/ncomms11658.
Mixed-signal integration BAC Reportersynthetic gene circuitsynthetic gene circuit (Other) Lu Synthetic mixed-signal computation in living cells. Nat Commun. 2016 Jun 3;7:11658. doi: 10.1038/ncomms11658.
pZS2oxySp-GFP-proD-oxyRsynthetic gene circuitsynthetic gene circuit (Other) Lu Synthetic mixed-signal computation in living cells. Nat Commun. 2016 Jun 3;7:11658. doi: 10.1038/ncomms11658.
Bxbi GFP MCP Reportersynthetic gene circuitsynthetic gene circuit (Other) Lu Synthetic mixed-signal computation in living cells. Nat Commun. 2016 Jun 3;7:11658. doi: 10.1038/ncomms11658.
pBAC Bxbi TetRsynthetic gene circuitsynthetic gene circuit (Other) Lu Synthetic mixed-signal computation in living cells. Nat Commun. 2016 Jun 3;7:11658. doi: 10.1038/ncomms11658.
pZA1pLtetO-GFPsynthetic gene circuitsynthetic gene circuit (Other) Lu Synthetic mixed-signal computation in living cells. Nat Commun. 2016 Jun 3;7:11658. doi: 10.1038/ncomms11658.
pZS1(pLtetO-TP901-aav-proA-tetR)-katGp-PhiC31-proD-oxyRsynthetic gene circuitsynthetic gene circuit (Other) Lu Synthetic mixed-signal computation in living cells. Nat Commun. 2016 Jun 3;7:11658. doi: 10.1038/ncomms11658.
Bxbi+TP901 GFP Bandpass BAC Reportersynthetic gene circuitsynthetic circuit (Other) Lu Synthetic mixed-signal computation in living cells. Nat Commun. 2016 Jun 3;7:11658. doi: 10.1038/ncomms11658.
pHIV_pTp66p51Expresses HIV reverse transcriptasep66 (Synthetic), p51 (Synthetic), LacI (Synthetic)Bacterial Expression Voigt Genetic encoding of DNA nanostructures and their self-assembly in living bacteria. Nat Commun. 2016 Apr 19;7:11179. doi: 10.1038/ncomms11179.
pMLRTExpresses Murine Leukemia Reverse Transcriptase (MLRT)Murine leukemia reverse transcriptase (Synthetic)Bacterial Expression Voigt Genetic encoding of DNA nanostructures and their self-assembly in living bacteria. Nat Commun. 2016 Apr 19;7:11179. doi: 10.1038/ncomms11179.
pO_72Transcripts the r_oligo part that includes the HIV reverse transcriptase binding site (HTBS)r_oligo (Synthetic)Bacterial Expression Voigt Genetic encoding of DNA nanostructures and their self-assembly in living bacteria. Nat Commun. 2016 Apr 19;7:11179. doi: 10.1038/ncomms11179.
pSEVA331BbpSEVA331 with Biobrick multiple cloning site Ellis Engineering control of bacterial cellulose production using a genetic toolkit and a new cellulose-producing strain. Proc Natl Acad Sci U S A. 2016 Jun 14;113(24):E3431-40. doi: 10.1073/pnas.1522985113. Epub 2016 May 31.
J23100-mRFP1-331BbmRFP1 behind constitutive promoter J23100 in pSEVA331Bb backboneJ23100-mRFP1 Ellis Engineering control of bacterial cellulose production using a genetic toolkit and a new cellulose-producing strain. Proc Natl Acad Sci U S A. 2016 Jun 14;113(24):E3431-40. doi: 10.1073/pnas.1522985113. Epub 2016 May 31.
J23101-mRFP1-331BbmRFP1 behind constitutive promoter J23101 in pSEVA331Bb backboneJ23101-mRFP1 Ellis Engineering control of bacterial cellulose production using a genetic toolkit and a new cellulose-producing strain. Proc Natl Acad Sci U S A. 2016 Jun 14;113(24):E3431-40. doi: 10.1073/pnas.1522985113. Epub 2016 May 31.