| pJRL2 PHO5pr VYFP (EB#1547) | | PHO5 promoter (Saccharomyces cerevisiae) | Yeast Expression |
O'Shea
| Control of stochasticity in eukaryotic gene expression.
Science. 2004 Jun 18. 304(5678):1811-4.
|
| pJRL2 PHO5(UASm1)pr VYFP (EB#1549) | | PHO5 promoter UASm1 (Saccharomyces cerevisiae) | Yeast Expression |
O'Shea
| Control of stochasticity in eukaryotic gene expression.
Science. 2004 Jun 18. 304(5678):1811-4.
|
| pJRL2 PHO5(TATA-C2)pr ECFP (EB#1562) | | PHO5 promoter TATA-C2 (Saccharomyces cerevisiae) | Yeast Expression |
O'Shea
| Control of stochasticity in eukaryotic gene expression.
Science. 2004 Jun 18. 304(5678):1811-4.
|
| pJRL2 PHO5(TATA-A1T6)pr ECFP (EB#1560) | | PHO5 promoter TATA-A1T6 (Saccharomyces cerevisiae) | Yeast Expression |
O'Shea
| Control of stochasticity in eukaryotic gene expression.
Science. 2004 Jun 18. 304(5678):1811-4.
|
| pJRL2 GAL1pr VYFP (EB#1571) | | GAL1 promoter (Saccharomyces cerevisiae) | Yeast Expression |
O'Shea
| Control of stochasticity in eukaryotic gene expression.
Science. 2004 Jun 18. 304(5678):1811-4.
|
| pJRL2 GAL1(TATA-A1T6)pr VYFP (EB#1573) | | GAL1 promoter TATA-A1T6 (Saccharomyces cerevisiae) | Yeast Expression |
O'Shea
| Control of stochasticity in eukaryotic gene expression.
Science. 2004 Jun 18. 304(5678):1811-4.
|
| pJRL2 GAL1(TATA-A1T6)pr ECFP (EB#1574) | | GAL1 promoter TATA-A1T6 (Saccharomyces cerevisiae) | Yeast Expression |
O'Shea
| Control of stochasticity in eukaryotic gene expression.
Science. 2004 Jun 18. 304(5678):1811-4.
|
| pJRL2 GAL1(TATA-C2)pr VYFP (EB#1577) | | GAL1 promoter TATA-C2 (Saccharomyces cerevisiae) | Yeast Expression |
O'Shea
| Control of stochasticity in eukaryotic gene expression.
Science. 2004 Jun 18. 304(5678):1811-4.
|
| pJRL2 GAL1(TATA-C2)pr ECFP (EB#1578) | | GAL1 promoter TATA-C2 (Saccharomyces cerevisiae) | Yeast Expression |
O'Shea
| Control of stochasticity in eukaryotic gene expression.
Science. 2004 Jun 18. 304(5678):1811-4.
|
| pPro29b(+) | | | Bacterial Expression, Synthetic Biology |
Keasling
|
Keasling Lab
(unpublished)
|
| pMLLKA-J72016 | | N terminal 6X His tag with RBS | Synthetic Biology ; 2ab assembly vector |
Anderson
|
J.C. Anderson Lab Plasmids
(unpublished)
|
| pMLLKA-J72017 | | N terminal avi tag with RBS | Synthetic Biology ; 2ab assembly vector |
Anderson
|
J.C. Anderson Lab Plasmids
(unpublished)
|
| pMLLKA-J72018 | | N terminal E tag with RBS | Synthetic Biology ; 2ab assembly vector |
Anderson
|
J.C. Anderson Lab Plasmids
(unpublished)
|
| pMLLKA-J72019 | | N terminal Flag tag with RBS | Synthetic Biology ; 2ab assembly vector |
Anderson
|
J.C. Anderson Lab Plasmids
(unpublished)
|
| pMLLKA-J72020 | | N terminal HA tag with RBS | Synthetic Biology ; 2ab assembly vector |
Anderson
|
J.C. Anderson Lab Plasmids
(unpublished)
|
| pMLLKA-J72021 | | N terminal HSV tag with RBS | Synthetic Biology ; 2ab assembly vector |
Anderson
|
J.C. Anderson Lab Plasmids
(unpublished)
|
| pMLLKA-J72022 | | N terminal myc tag with RBS | Synthetic Biology ; 2ab assembly vector |
Anderson
|
J.C. Anderson Lab Plasmids
(unpublished)
|
| pMLLKA-J72023 | | N terminal S tag with RBS | Synthetic Biology ; 2ab assembly vector |
Anderson
|
J.C. Anderson Lab Plasmids
(unpublished)
|
| pMLLKA-J72024 | | N terminal Strep tag with RBS | Synthetic Biology ; 2ab assembly vector |
Anderson
|
J.C. Anderson Lab Plasmids
(unpublished)
|
| pMLLKA-J72025 | | N terminal T7 tag with RBS | Synthetic Biology ; 2ab assembly vector |
Anderson
|
J.C. Anderson Lab Plasmids
(unpublished)
|
| pMLLKA-J72026 | | N terminal V5 tag with RBS | Synthetic Biology ; 2ab assembly vector |
Anderson
|
J.C. Anderson Lab Plasmids
(unpublished)
|
| pMLLKA-J72027 | | N terminal VSV tag with RBS | Synthetic Biology ; 2ab assembly vector |
Anderson
|
J.C. Anderson Lab Plasmids
(unpublished)
|
| pMLLCK-J72028 | | C terminal 6XHIS tag | Synthetic Biology ; 2ab assembly vector |
Anderson
|
J.C. Anderson Lab Plasmids
(unpublished)
|
| pMLLCK-J72029 | | C terminal avi tag | Synthetic Biology ; 2ab assembly vector |
Anderson
|
J.C. Anderson Lab Plasmids
(unpublished)
|
| pMLLCK-J72030 | | C terminal E tag | Synthetic Biology ; 2ab assembly vector |
Anderson
|
J.C. Anderson Lab Plasmids
(unpublished)
|
| pMLLCK-K112603 | | C terminal Flag tag | Synthetic Biology ; 2ab assembly vector |
Anderson
|
J.C. Anderson Lab Plasmids
(unpublished)
|
| pMLLCK-K112507 | | C terminal HA tag | Synthetic Biology ; 2ab assembly vector |
Anderson
|
J.C. Anderson Lab Plasmids
(unpublished)
|
| pMLLCK-J72031 | | C terminal HSV tag | Synthetic Biology ; 2ab assembly vector |
Anderson
|
J.C. Anderson Lab Plasmids
(unpublished)
|
| pMLLCK-J72032 | | C terminal S tag | Synthetic Biology ; 2ab assembly vector |
Anderson
|
J.C. Anderson Lab Plasmids
(unpublished)
|
| pMLLCK-J72033 | | C terminal Strep tag | Synthetic Biology ; 2ab assembly vector |
Anderson
|
J.C. Anderson Lab Plasmids
(unpublished)
|
| pMLLCK-J72034 | | C terminal T7 tag | Synthetic Biology ; 2ab assembly vector |
Anderson
|
J.C. Anderson Lab Plasmids
(unpublished)
|
| pMLLCK-J72035 | | C terminal V5 tag | Synthetic Biology ; 2ab assembly vector |
Anderson
|
J.C. Anderson Lab Plasmids
(unpublished)
|
| pMLLCK-J72036 | | C terminal VSV tag | Synthetic Biology ; 2ab assembly vector |
Anderson
|
J.C. Anderson Lab Plasmids
(unpublished)
|
| pMLLCK-J72037 | | Med7 aa102-205 for N-terminal tagging | Synthetic Biology ; 2ab assembly vector |
Anderson
|
J.C. Anderson Lab Plasmids
(unpublished)
|
| pMLLCK-J72038 | | Med21 aa1-132 for N-terminal tagging (Saccharomyces cerevisiae) | Synthetic Biology ; 2ab assembly vector |
Anderson
|
J.C. Anderson Lab Plasmids
(unpublished)
|
| pMLLKA-J72039 | | Med7 aa102-205 for C-terminal tagging (Saccharomyces cerevisiae) | Synthetic Biology ; 2ab assembly vector |
Anderson
|
J.C. Anderson Lab Plasmids
(unpublished)
|
| pMLLKA-J72040 | | Med21 aa1-132 for C-terminal tagging (Saccharomyces cerevisiae) | Synthetic Biology ; 2ab assembly vector |
Anderson
|
J.C. Anderson Lab Plasmids
(unpublished)
|
| pMLLAC-J72041 | | Tev Protease Cleavage site | Synthetic Biology ; 2ab assembly vector |
Anderson
|
J.C. Anderson Lab Plasmids
(unpublished)
|
| pUCBB-eGFP | | eGFP | Bacterial Expression, Synthetic Biology |
Schmidt-dannert
| Optimized compatible set of BioBrick vectors for metabolic pathway engineering.
Appl Microbiol Biotechnol. 2011 Dec;92(6):1275-86. Epub 2011 Oct 28.
|
| pBBRBB-eGFP | | eGFP | Bacterial Expression, Synthetic Biology |
Schmidt-dannert
| Optimized compatible set of BioBrick vectors for metabolic pathway engineering.
Appl Microbiol Biotechnol. 2011 Dec;92(6):1275-86. Epub 2011 Oct 28.
|
| pCDFBB-eGFP | | egfp | Synthetic Biology |
Schmidt-dannert
| Optimized compatible set of BioBrick vectors for metabolic pathway engineering.
Appl Microbiol Biotechnol. 2011 Dec;92(6):1275-86. Epub 2011 Oct 28.
|
| pACBB-eGFP | | eGFP | Synthetic Biology |
Schmidt-dannert
| Optimized compatible set of BioBrick vectors for metabolic pathway engineering.
Appl Microbiol Biotechnol. 2011 Dec;92(6):1275-86. Epub 2011 Oct 28.
|
| pUCBB-pT7-eGFP | | eGFP | Bacterial Expression, Synthetic Biology |
Schmidt-dannert
| Optimized compatible set of BioBrick vectors for metabolic pathway engineering.
Appl Microbiol Biotechnol. 2011 Dec;92(6):1275-86. Epub 2011 Oct 28.
|
| pUCBB-pBAD-eGFP | | eGFP | Synthetic Biology |
Schmidt-dannert
| Optimized compatible set of BioBrick vectors for metabolic pathway engineering.
Appl Microbiol Biotechnol. 2011 Dec;92(6):1275-86. Epub 2011 Oct 28.
|
| pUCBB-ptet-eGFP | | eGFP | Synthetic Biology |
Schmidt-dannert
| Optimized compatible set of BioBrick vectors for metabolic pathway engineering.
Appl Microbiol Biotechnol. 2011 Dec;92(6):1275-86. Epub 2011 Oct 28.
|
| pACBB-TetT-LVA | | TetR | Bacterial Expression, Synthetic Biology |
Schmidt-dannert
| Optimized compatible set of BioBrick vectors for metabolic pathway engineering.
Appl Microbiol Biotechnol. 2011 Dec;92(6):1275-86. Epub 2011 Oct 28.
|
| pUCBB-oriT | | oriT | Bacterial Expression, Synthetic Biology |
Schmidt-dannert
| Optimized compatible set of BioBrick vectors for metabolic pathway engineering.
Appl Microbiol Biotechnol. 2011 Dec;92(6):1275-86. Epub 2011 Oct 28.
|
| pUCBB-ntH6-eGFP | | eGFP | Bacterial Expression, Synthetic Biology |
Schmidt-dannert
| Optimized compatible set of BioBrick vectors for metabolic pathway engineering.
Appl Microbiol Biotechnol. 2011 Dec;92(6):1275-86. Epub 2011 Oct 28.
|
| pUCBB-ctH6-eGFP | | eGFP | Synthetic Biology |
Schmidt-dannert
| Optimized compatible set of BioBrick vectors for metabolic pathway engineering.
Appl Microbiol Biotechnol. 2011 Dec;92(6):1275-86. Epub 2011 Oct 28.
|
| pUCBB-lacZa | | LacZ-alpha | Bacterial Expression, Synthetic Biology |
Schmidt-dannert
| Optimized compatible set of BioBrick vectors for metabolic pathway engineering.
Appl Microbiol Biotechnol. 2011 Dec;92(6):1275-86. Epub 2011 Oct 28.
|
| pBbE8a-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbE8c-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbE8k-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbA8a-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbA8c-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbA8k-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbS8a-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbS8c-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbS8k-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbE5a-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbE5c-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbE5k-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbA5a-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbA5c-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbA5k-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbS5a-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbS5c-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbS5k-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbE6a-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbE6c-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbE6k-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbA6a-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbA6c-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbA6k-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbS6a-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbS6c-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbS6k-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbE4a-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbE4c-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbE4k-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbA4a-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbA4c-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbA4k-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbS4a-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbS4c-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbS4k-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbE3a-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbE3c-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbE3k-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbA3a-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbA3c-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbA3k-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbS3a-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbS3c-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbS3k-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbE7a-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbE7c-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbE7k-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbA7a-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbA7c-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbA7k-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbS7c-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbS7k-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbE2a-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbE2c-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbE2k-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbA2a-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbA2c-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbA2k-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbS2a-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbS2c-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbS2k-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbE1a-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbE1c-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbE1k-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbA1a-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbA1c-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbA1k-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbS1a-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbS1c-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbS1k-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbB1a-GFP | | GFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbB1c-GFP | | GFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbB1k-GFP | | GFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbB2a-GFP | | GFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbB2c-GFP | | GFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbB2k-GFP | | GFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbB3a-GFP | | GFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbB3c-GFP | | GFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbB3k-GFP | | GFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbB4a-GFP | | GFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbB4c-GFP | | GFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbB4k-GFP | | GFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbB5a-GFP | | GFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbB5c-GFP | | GFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbB5k-GFP | | GFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbB6a-GFP | | GFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbB6c-GFP | | GFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbB6k-GFP | | GFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbB7a-GFP | | GFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbB7c-GFP | | GFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbB7k-GFP | | GFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbB8a-GFP | | GFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbB8c-GFP | | GFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbB8k-GFP | | GFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbE0c-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbE0k-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbB0c-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbS0a-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbS0k-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbB0k-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbB0a-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbE0a-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbA0c-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbA0k-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pBbA0a-RFP | | RFP (Synthetic) | Bacterial Expression, Synthetic Biology |
Keasling
| BglBrick vectors and datasheets: A synthetic biology platform for gene expression.
J Biol Eng. 2011 Sep 20;5:12.
|
| pRibo BsaHind | | | Bacterial Expression, Synthetic Biology ; Mycobacteria replicating vector |
Bertozzi
| A riboswitch-based inducible gene expression system for mycobacteria.
PLoS One. 2012;7(1):e29266. Epub 2012 Jan 18.
|
| pRibo EcoHind | | | Bacterial Expression, Synthetic Biology ; Mycobacteria replicating vector |
Bertozzi
| A riboswitch-based inducible gene expression system for mycobacteria.
PLoS One. 2012;7(1):e29266. Epub 2012 Jan 18.
|
| pRiboS BsaHind | | | Bacterial Expression, Synthetic Biology ; Mycobacteria replicating vector |
Bertozzi
| A riboswitch-based inducible gene expression system for mycobacteria.
PLoS One. 2012;7(1):e29266. Epub 2012 Jan 18.
|
| pRiboS EcoHind | | | Bacterial Expression, Synthetic Biology |
Bertozzi
| A riboswitch-based inducible gene expression system for mycobacteria.
PLoS One. 2012;7(1):e29266. Epub 2012 Jan 18.
|
| pST5552 | Mycobacteria replicating vector expressing eGFP. | eGFP | Bacterial Expression, Synthetic Biology ; Mycobacteria replicating vector |
Bertozzi
| A riboswitch-based inducible gene expression system for mycobacteria.
PLoS One. 2012;7(1):e29266. Epub 2012 Jan 18.
|
| pST5832 | | lacZ | Bacterial Expression, Synthetic Biology ; Mycobacteria replicating vector |
Bertozzi
| A riboswitch-based inducible gene expression system for mycobacteria.
PLoS One. 2012;7(1):e29266. Epub 2012 Jan 18.
|
| BBa_J72110-BBa_J72094 | | AraC-pBad + coi | Bacterial Expression, Synthetic Biology |
Anderson
| Scalable plasmid transfer using engineered P1-based phagemids.
ACS Synth Biol. 2012 Dec 21;1(12):583-9. doi: 10.1021/sb300054p. Epub 2012 Aug 30.
|
| BBa_J72110-BBa_J72097 | | RhaRS-pRhaB + Coi | Bacterial Expression, Synthetic Biology |
Anderson
| Scalable plasmid transfer using engineered P1-based phagemids.
ACS Synth Biol. 2012 Dec 21;1(12):583-9. doi: 10.1021/sb300054p. Epub 2012 Aug 30.
|
| BBa_J72110-BBa_J72103 | | AraC-pBad + coi + cin + repL + pacA | Bacterial Expression, Synthetic Biology ; Phagemid |
Anderson
| Scalable plasmid transfer using engineered P1-based phagemids.
ACS Synth Biol. 2012 Dec 21;1(12):583-9. doi: 10.1021/sb300054p. Epub 2012 Aug 30.
|
| BBa_J72114-BBa_J72100 | | lacZ | Bacterial Expression, Synthetic Biology ; Phagemid |
Anderson
| Scalable plasmid transfer using engineered P1-based phagemids.
ACS Synth Biol. 2012 Dec 21;1(12):583-9. doi: 10.1021/sb300054p. Epub 2012 Aug 30.
|
| BBa_J72214-BBa_J72090 | | VioABCDE | Bacterial Expression, Synthetic Biology ; phagemid |
Anderson
| Scalable plasmid transfer using engineered P1-based phagemids.
ACS Synth Biol. 2012 Dec 21;1(12):583-9. doi: 10.1021/sb300054p. Epub 2012 Aug 30.
|
| BBa_J72114-BBa_J72104 | | RhaRS + pRhaB + LuxCDABE, VioABCE, LacZ | Bacterial Expression, Luciferase, Synthetic Biology ; Phagemid |
Anderson
| Scalable plasmid transfer using engineered P1-based phagemids.
ACS Synth Biol. 2012 Dec 21;1(12):583-9. doi: 10.1021/sb300054p. Epub 2012 Aug 30.
|
| BBa_J72113-BBa_J72152 | | Phagemid + GFP | Bacterial Expression, Synthetic Biology |
Anderson
| Scalable plasmid transfer using engineered P1-based phagemids.
ACS Synth Biol. 2012 Dec 21;1(12):583-9. doi: 10.1021/sb300054p. Epub 2012 Aug 30.
|
| BBa_J72113-BBa_J72103 | | AraC-pBad + coi + cin + repL + pacA | Bacterial Expression, Synthetic Biology |
Anderson
| Scalable plasmid transfer using engineered P1-based phagemids.
ACS Synth Biol. 2012 Dec 21;1(12):583-9. doi: 10.1021/sb300054p. Epub 2012 Aug 30.
|
| pNH001 | | TAP-tag (Caenorhabditis elegans) | Worm Expression |
Dolphin
| A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones.
BMC Biotechnol. 2013 Jan 3;13(1):1.
|
| pNH002 | | N-TAP TAG::[(G4S)3]::mTFP1 (Caenorhabditis elegans) | Worm Expression |
Dolphin
| A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones.
BMC Biotechnol. 2013 Jan 3;13(1):1.
|
| pNH009 | | N-TAP-tag::mTFP1 (Caenorhabditis elegans) | Worm Expression |
Dolphin
| A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones.
BMC Biotechnol. 2013 Jan 3;13(1):1.
|
| pNH013 | | mTFP1 (Caenorhabditis elegans) | Synthetic Biology |
Dolphin
| A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones.
BMC Biotechnol. 2013 Jan 3;13(1):1.
|
| pNH026 | | mTFP1::linker::TAP TAG (Caenorhabditis elegans) | |
Dolphin
| A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones.
BMC Biotechnol. 2013 Jan 3;13(1):1.
|
| pNH030 | | mTFP1::linker::2xNLS (Caenorhabditis elegans) | Worm Expression |
Dolphin
| A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones.
BMC Biotechnol. 2013 Jan 3;13(1):1.
|
| pNH034 | | RT-cassette (Synthetic) | |
Dolphin
| A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones.
BMC Biotechnol. 2013 Jan 3;13(1):1.
|
| pNH039 | | CFP | |
Dolphin
| A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones.
BMC Biotechnol. 2013 Jan 3;13(1):1.
|
| pNH040 | | GFP | |
Dolphin
| A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones.
BMC Biotechnol. 2013 Jan 3;13(1):1.
|
| pNH041 | | YFP | |
Dolphin
| A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones.
BMC Biotechnol. 2013 Jan 3;13(1):1.
|
| pNH042 | | mCherry | |
Dolphin
| A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones.
BMC Biotechnol. 2013 Jan 3;13(1):1.
|
| pNH043 | | mTFP1 | |
Dolphin
| A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones.
BMC Biotechnol. 2013 Jan 3;13(1):1.
|
| pNH050 | | CFP-RT-CFP | |
Dolphin
| A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones.
BMC Biotechnol. 2013 Jan 3;13(1):1.
|
| pNH051 | | GFP-RT-GFP | |
Dolphin
| A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones.
BMC Biotechnol. 2013 Jan 3;13(1):1.
|
| pNH052 | | YFP-RT-YFP | |
Dolphin
| A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones.
BMC Biotechnol. 2013 Jan 3;13(1):1.
|
| pNH053 | | mCherry-RT-mCherry | |
Dolphin
| A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones.
BMC Biotechnol. 2013 Jan 3;13(1):1.
|
| pNH054 | | mTFP1-RT-mTFP1 | |
Dolphin
| A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones.
BMC Biotechnol. 2013 Jan 3;13(1):1.
|
| pNH058 | | TAP Tag::mTFP1 | |
Dolphin
| A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones.
BMC Biotechnol. 2013 Jan 3;13(1):1.
|
| pNH066 | | N-TAP Tag-RT-::mTFP1 (Caenorhabditis elegans) | |
Dolphin
| A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones.
BMC Biotechnol. 2013 Jan 3;13(1):1.
|
| pNH078 | | mTFP1 (Caenorhabditis elegans) | Worm Expression |
Dolphin
| A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones.
BMC Biotechnol. 2013 Jan 3;13(1):1.
|
| pNH082 | | mTFP1 (Caenorhabditis elegans) | Worm Expression |
Dolphin
| A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones.
BMC Biotechnol. 2013 Jan 3;13(1):1.
|
| pNH090 | | mTFP1::[(G4S)3]::C-TAP Tag (Caenorhabditis elegans) | |
Dolphin
| A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones.
BMC Biotechnol. 2013 Jan 3;13(1):1.
|
| pNH094 | | mTFP1-RT-::[(G4S)3]::C-TAP Tag (Caenorhabditis elegans) | |
Dolphin
| A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones.
BMC Biotechnol. 2013 Jan 3;13(1):1.
|
| pCsy4 | | csy4 gene | Bacterial Expression, Synthetic Biology |
Arkin
| RNA processing enables predictable programming of gene expression.
Nat Biotechnol. 2012 Oct;30(10):1002-6. doi: 10.1038/nbt.2355. Epub 2012 Sep 16.
|
| BB-ÂNIC-ÂII-ÂHisN-ÂTET12 | | TET12 (Synthetic) | Bacterial Expression, Synthetic Biology |
Jerala
| Design of a single-chain polypeptide tetrahedron assembled from coiled-coil segments.
Nat Chem Biol. 2013 Apr 28. doi: 10.1038/nchembio.1248.
|
| pBADmod1-linker2-gamS | | gamS (Other) | Bacterial Expression, Synthetic Biology |
Murray
|
Murray Lab gamS and ClpX plasmids
(unpublished)
|
| pE-FLP | | flp | Bacterial Expression, Synthetic Biology |
Endy
| One-step cloning and chromosomal integration of DNA.
ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
|
| pOSIP-CH (CamR, HK022) | | | Bacterial Expression, Synthetic Biology |
Endy
| One-step cloning and chromosomal integration of DNA.
ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
|
| pOSIP-CT (CamR, P21) | | | Bacterial Expression, Synthetic Biology |
Endy
| One-step cloning and chromosomal integration of DNA.
ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
|
| pOSIP-KC (KanR, PhiC31) | | | Bacterial Expression, Synthetic Biology |
Endy
| One-step cloning and chromosomal integration of DNA.
ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
|
| pOSIP-KH (KanR, HK022) | | | Bacterial Expression, Synthetic Biology |
Endy
| One-step cloning and chromosomal integration of DNA.
ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
|
| pOSIP-KL (KanR, Lambda) | | | Bacterial Expression, Synthetic Biology |
Endy
| One-step cloning and chromosomal integration of DNA.
ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
|
| pOSIP-KO (KanR, 186) | | | Bacterial Expression, Synthetic Biology |
Endy
| One-step cloning and chromosomal integration of DNA.
ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
|
| pOSIP-KP (KanR, Phi80) | | | Bacterial Expression, Synthetic Biology |
Endy
| One-step cloning and chromosomal integration of DNA.
ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
|
| pOSIP-KT (KanR, P21) | | | Bacterial Expression, Synthetic Biology |
Endy
| One-step cloning and chromosomal integration of DNA.
ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
|
| pOSIP-TH (TetR, HK022) | | | Bacterial Expression, Synthetic Biology |
Endy
| One-step cloning and chromosomal integration of DNA.
ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
|
| pOSIP-TT (TetR, P21) | | | Bacterial Expression, Synthetic Biology |
Endy
| One-step cloning and chromosomal integration of DNA.
ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.
|
| pGR | Reporter plasmid for measuring terminator strength by inserting terminator of interest between the GFP and RFP, expression driven by a single PBAD promoter inducible by arabinose | | Bacterial Expression, Synthetic Biology |
Voigt
| Characterization of 582 natural and synthetic terminators and quantification of their design constraints.
Nat Methods. 2013 Jun 2. doi: 10.1038/nmeth.2515.
|
| pCru5-ACC/ACC/ACC/ACC-mEGFP-IRES-mCherry | Retroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation. | mEGFP (Synthetic), mCherry (Synthetic) | Mammalian Expression, Retroviral, Synthetic Biology |
Wang
| Tuning gene expression with synthetic upstream open reading frames.
Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24.
|
| pCru5-ACC/ACC/ACC-mEGFP-IRES-mCherry | Retroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation. | mEGFP (Synthetic), mCherry (Synthetic) | Mammalian Expression, Retroviral, Synthetic Biology |
Wang
| Tuning gene expression with synthetic upstream open reading frames.
Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24.
|
| pCru5-ACC/ACC-mEGFP-IRES-mCherry | Retroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation. | mEGFP (Synthetic), mCherry (Synthetic) | Mammalian Expression, Retroviral, Synthetic Biology |
Wang
| Tuning gene expression with synthetic upstream open reading frames.
Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24.
|
| pCru5-GGG/ACC-mEGFP-IRES-mCherry | Retroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation. | mEGFP (Synthetic), mCherry (Synthetic) | Mammalian Expression, Retroviral, Synthetic Biology |
Wang
| Tuning gene expression with synthetic upstream open reading frames.
Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24.
|
| pCru5-CCC/ACC-mEGFP-IRES-mCherry | Retroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation. | mEGFP (Synthetic), mCherry (Synthetic) | Mammalian Expression, Retroviral, Synthetic Biology |
Wang
| Tuning gene expression with synthetic upstream open reading frames.
Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24.
|
| pCru5-UCU/ACC-mEGFP-IRES-mCherry | Retroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation. | mEGFP (Synthetic), mCherry (Synthetic) | Mammalian Expression, Retroviral, Synthetic Biology |
Wang
| Tuning gene expression with synthetic upstream open reading frames.
Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24.
|
| pCru5-/CGA-mEGFP-IRES-mCherry | Retroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation. | mEGFP (Synthetic), mCherry (Synthetic) | Mammalian Expression, Retroviral, Synthetic Biology |
Wang
| Tuning gene expression with synthetic upstream open reading frames.
Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24.
|
| pCru5-/UAA-mEGFP-IRES-mCherry | Retroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation. | mEGFP (Synthetic), mCherry (Synthetic) | Mammalian Expression, Retroviral, Synthetic Biology |
Wang
| Tuning gene expression with synthetic upstream open reading frames.
Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24.
|
| pCru5-/GCCACC-mEGFP-IRES-mCherry | Retroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation. | mEGFP (Synthetic), mCherry (Synthetic) | Mammalian Expression, Retroviral, Synthetic Biology |
Wang
| Tuning gene expression with synthetic upstream open reading frames.
Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24.
|
| pWPT-ACC/ACC/ACC/ACC-mEGFP-IRES-mCherry | Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation. | mEGFP (Synthetic), mCherry (Synthetic) | Mammalian Expression, Lentiviral, Synthetic Biology |
Wang
| Tuning gene expression with synthetic upstream open reading frames.
Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24.
|
| pWPT-ACC/ACC/ACC-mEGFP-IRES-mCherry | Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation. | mEGFP (Synthetic), mCherry (Synthetic) | Mammalian Expression, Lentiviral, Synthetic Biology |
Wang
| Tuning gene expression with synthetic upstream open reading frames.
Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24.
|
| pWPT-ACC/ACC-mEGFP-IRES-mCherry | Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation. | mEGFP (Synthetic), mCherry (Synthetic) | Mammalian Expression, Lentiviral, Synthetic Biology |
Wang
| Tuning gene expression with synthetic upstream open reading frames.
Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24.
|
| pWPT-GGG/ACC-mEGFP-IRES-mCherry | Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation. | mEGFP (Synthetic), mCherry (Synthetic) | Mammalian Expression, Lentiviral, Synthetic Biology |
Wang
| Tuning gene expression with synthetic upstream open reading frames.
Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24.
|
| pWPT-CCC/ACC-mEGFP-IRES-mCherry | Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation. | mEGFP (Synthetic), mCherry (Synthetic) | Mammalian Expression, Lentiviral, Synthetic Biology |
Wang
| Tuning gene expression with synthetic upstream open reading frames.
Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24.
|
| pWPT-UCU/ACC-mEGFP-IRES-mCherry | Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation. | mEGFP (Synthetic), mCherry (Synthetic) | Mammalian Expression, Lentiviral, Synthetic Biology |
Wang
| Tuning gene expression with synthetic upstream open reading frames.
Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24.
|
| pWPT-/CGA-mEGFP-IRES-mCherry | Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation. | mEGFP (Synthetic), mCherry (Synthetic) | Mammalian Expression, Lentiviral, Synthetic Biology |
Wang
| Tuning gene expression with synthetic upstream open reading frames.
Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24.
|
| pWPT-/UAA-mEGFP-IRES-mCherry | Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation. | mEGFP (Synthetic), mCherry (Synthetic) | Mammalian Expression, Lentiviral, Synthetic Biology |
Wang
| Tuning gene expression with synthetic upstream open reading frames.
Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24.
|
| pWPT-/GCCACC-mEGFP-IRES-mCherry | Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation. | mEGFP (Synthetic), mCherry (Synthetic) | Mammalian Expression, Lentiviral, Synthetic Biology |
Wang
| Tuning gene expression with synthetic upstream open reading frames.
Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24.
|
| pBS-67Sma | Chlamy Cp transformation for transgene integration via double-homologous recombination into the rbcL-psaB intergenic region | EcoRI 5.8 kB (Other) | Synthetic Biology |
Melis
| Marker-free genetic engineering of the chloroplast in the green microalga Chlamydomonas reinhardtii.
Plant Biotechnol J. 2013 Sep;11(7):818-28. doi: 10.1111/pbi.12073. Epub 2013 May 7.
|
| pJ207 Delta-cpc-KanR | Deletes the cpc operon in Synechocystis and confers kanamycin resistance | Replacing the cpc operon with kanamycin resistance (Synthetic) | Synthetic Biology |
Melis
| Maximizing photosynthetic efficiency and culture productivity in cyanobacteria upon minimizing the phycobilisome light-harvesting antenna size.
Biochim Biophys Acta. 2014 Oct;1837(10):1653-64. doi: 10.1016/j.bbabio.2014.07.009. Epub 2014 Jul 18.
|
| pBullet-cyt-n | destination vector with cytosol marker (ECFP) for tagged fluorescent protein (N-ter-EYFP) of plant gene | | Synthetic Biology |
Heazlewood
| The Plant Glycosyltransferase Clone Collection for Functional Genomics.
Plant J. 2014 Jun 6. doi: 10.1111/tpj.12577.
|
| pBullet-cyt-c | destination vector with cytosol marker (ECFP) for tagged fluorescent protein (C-ter-EYFP) of plant gene | | Synthetic Biology |
Heazlewood
| The Plant Glycosyltransferase Clone Collection for Functional Genomics.
Plant J. 2014 Jun 6. doi: 10.1111/tpj.12577.
|
| pBullet-er-n | destination vector with WAK2 (29 aa)-ECFP- ER marker (HDEL) for tagged fluorescent protein (N-ter-EYFP) of plant gene | | Synthetic Biology |
Heazlewood
| The Plant Glycosyltransferase Clone Collection for Functional Genomics.
Plant J. 2014 Jun 6. doi: 10.1111/tpj.12577.
|
| pBullet-er-c | destination vector with WAK2 (29 aa)-ECFP- ER marker (HDEL) for tagged fluorescent protein (C-ter-EYFP) of plant gene | | Synthetic Biology |
Heazlewood
| The Plant Glycosyltransferase Clone Collection for Functional Genomics.
Plant J. 2014 Jun 6. doi: 10.1111/tpj.12577.
|
| pBullet-cg-n | destination vector with cis-golgi marker (alpha-man I 49AA) -ECFP for tagged fluorescent protein (N-ter-EYFP) of plant gene | | Synthetic Biology |
Heazlewood
| The Plant Glycosyltransferase Clone Collection for Functional Genomics.
Plant J. 2014 Jun 6. doi: 10.1111/tpj.12577.
|
| pBullet-cg-c | destination vector with cis-golgi marker (alpha-man I 49AA) -ECFP for tagged fluorescent protein (C-ter-EYFP) of plant gene | | Synthetic Biology |
Heazlewood
| The Plant Glycosyltransferase Clone Collection for Functional Genomics.
Plant J. 2014 Jun 6. doi: 10.1111/tpj.12577.
|
| pBullet-tgn-n | destination vector with ECFP-trans golgi network marker (VTI12) for tagged fluorescent protein (N-ter-EYFP) of plant gene | | Synthetic Biology |
Heazlewood
| The Plant Glycosyltransferase Clone Collection for Functional Genomics.
Plant J. 2014 Jun 6. doi: 10.1111/tpj.12577.
|
| pBullet-tgn-c | destination vector with ECFP-trans golgi network marker (VTI12) for tagged fluorescent protein (C-ter-EYFP) of plant gene | | Synthetic Biology |
Heazlewood
| The Plant Glycosyltransferase Clone Collection for Functional Genomics.
Plant J. 2014 Jun 6. doi: 10.1111/tpj.12577.
|
| pBullet-end-n | destination vector with ECFP- endosome marker (RabF2a) for tagged fluorescent protein (N-ter-EYFP) of plant gene | | Synthetic Biology |
Heazlewood
| The Plant Glycosyltransferase Clone Collection for Functional Genomics.
Plant J. 2014 Jun 6. doi: 10.1111/tpj.12577.
|
| pBullet-end-c | destination vector with ECFP- endosome marker (RabF2a) for tagged fluorescent protein (C-ter-EYFP) of plant gene | | Synthetic Biology |
Heazlewood
| The Plant Glycosyltransferase Clone Collection for Functional Genomics.
Plant J. 2014 Jun 6. doi: 10.1111/tpj.12577.
|
| pBullet-vac-n | destination vector with vacuole marker (gamma-TIP) - ECFP for tagged fluorescent protein (N-ter-EYFP) of plant gene | | Synthetic Biology |
Heazlewood
| The Plant Glycosyltransferase Clone Collection for Functional Genomics.
Plant J. 2014 Jun 6. doi: 10.1111/tpj.12577.
|
| pBullet-vac-c | destination vector with vacuole marker (gamma-TIP) - ECFP for tagged fluorescent protein (C-ter-EYFP) of plant gene | | Synthetic Biology |
Heazlewood
| The Plant Glycosyltransferase Clone Collection for Functional Genomics.
Plant J. 2014 Jun 6. doi: 10.1111/tpj.12577.
|
| pBullet-pm-n | destination vector with PIP2a-ECFP (plasma membrane marker) for tagged fluorescent protein (N-ter-EYFP) of plant gene | | Synthetic Biology |
Heazlewood
| The Plant Glycosyltransferase Clone Collection for Functional Genomics.
Plant J. 2014 Jun 6. doi: 10.1111/tpj.12577.
|
| pBullet-pm-c | destination vector with PIP2a-ECFP (plasma membrane marker) for tagged fluorescent protein (C-ter-EYFP) of plant gene | | Synthetic Biology |
Heazlewood
| The Plant Glycosyltransferase Clone Collection for Functional Genomics.
Plant J. 2014 Jun 6. doi: 10.1111/tpj.12577.
|
| pCS1 | Produces aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase. | KanR (Other) | Bacterial Expression |
Bennett
| Stable maintenance of multiple plasmids in E. coli using a single selective marker.
ACS Synth Biol. 2012 Oct 19;1(10):445-50. doi: 10.1021/sb3000589. Epub 2012 Sep 4.
|
| pCS5 | Produces one half (beta) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase. | KanR (Other) | Bacterial Expression |
Bennett
| Stable maintenance of multiple plasmids in E. coli using a single selective marker.
ACS Synth Biol. 2012 Oct 19;1(10):445-50. doi: 10.1021/sb3000589. Epub 2012 Sep 4.
|
| pCS4 | Produces one half (alpha) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase. | KanR (Other) | Bacterial Expression |
Bennett
| Stable maintenance of multiple plasmids in E. coli using a single selective marker.
ACS Synth Biol. 2012 Oct 19;1(10):445-50. doi: 10.1021/sb3000589. Epub 2012 Sep 4.
|
| pCS6 | Produces T7 RNA Polymerase. | T7 RNA Polymerase (Other) | Bacterial Expression |
Bennett
| Stable maintenance of multiple plasmids in E. coli using a single selective marker.
ACS Synth Biol. 2012 Oct 19;1(10):445-50. doi: 10.1021/sb3000589. Epub 2012 Sep 4.
|
| pSEVAT1Z1A | GeneGuard vector cassette: thyA, R6K COR, Zeta toxin; with ampR as cargo. | | Bacterial Expression, Synthetic Biology |
Ellis
| GeneGuard: a Modular Plasmid System Designed for Biosafety.
ACS Synth Biol. 2014 May 13.
|
| pSEVAD1Z1A | GeneGuard vector cassette: dapA, R6K COR, Zeta toxin; with ampR as cargo. | | Bacterial Expression, Synthetic Biology |
Ellis
| GeneGuard: a Modular Plasmid System Designed for Biosafety.
ACS Synth Biol. 2014 May 13.
|
| pSEVAT1K1A | GeneGuard vector cassette: thyA, R6K COR, Kid toxin; with ampR as cargo. | | Bacterial Expression, Synthetic Biology |
Ellis
| GeneGuard: a Modular Plasmid System Designed for Biosafety.
ACS Synth Biol. 2014 May 13.
|
| pSEVAD1K1A | GeneGuard vector cassette: dapA, R6K COR, Kid toxin; with ampR as cargo. | | Bacterial Expression, Synthetic Biology |
Ellis
| GeneGuard: a Modular Plasmid System Designed for Biosafety.
ACS Synth Biol. 2014 May 13.
|
| pSEVAT7Z1A | GeneGuard vector cassette: thyA, ColE2 COR, Zeta toxin; with ampR as cargo. | | Bacterial Expression, Synthetic Biology |
Ellis
| GeneGuard: a Modular Plasmid System Designed for Biosafety.
ACS Synth Biol. 2014 May 13.
|
| pSEVAD7Z1A | GeneGuard vector cassette: dapA, ColE2 COR, Zeta toxin; with ampR as cargo. | | Bacterial Expression, Synthetic Biology |
Ellis
| GeneGuard: a Modular Plasmid System Designed for Biosafety.
ACS Synth Biol. 2014 May 13.
|
| pSEVAT7K1A | GeneGuard vector cassette: thyA, ColE2 COR, Kid toxin; with ampR as cargo. | | Bacterial Expression, Synthetic Biology |
Ellis
| GeneGuard: a Modular Plasmid System Designed for Biosafety.
ACS Synth Biol. 2014 May 13.
|
| pSEVAD7K1A | GeneGuard vector cassette: dapA, ColE2 COR, Kid toxin; with ampR as cargo. | | Bacterial Expression, Synthetic Biology |
Ellis
| GeneGuard: a Modular Plasmid System Designed for Biosafety.
ACS Synth Biol. 2014 May 13.
|
| pSEVA12DKR-A | GeneGuard genomic cassette: for constructing ΔdapA::RBS A pi, Kis, FRT-kanR-FRT (low-copy support for R6K COR). Will support pSEVAD1K. | Genomic cassette (Synthetic) | Bacterial Expression, Synthetic Biology |
Ellis
| GeneGuard: a Modular Plasmid System Designed for Biosafety.
ACS Synth Biol. 2014 May 13.
|
| pSEVA12DKR-E | GeneGuard genomic cassette: for constructing ΔdapA::RBS E pi, Kis, FRT-kanR-FRT (medium-copy support for R6K COR). Will support pSEVAD1K. | Genomic cassette (Synthetic) | Bacterial Expression, Synthetic Biology |
Ellis
| GeneGuard: a Modular Plasmid System Designed for Biosafety.
ACS Synth Biol. 2014 May 13.
|
| pSEVA12DKR-J | GeneGuard genomic cassette: for constructing ΔdapA::RBS J pi, Kis, FRT-kanR-FRT (high-copy support for R6K COR). Will support pSEVAD1K. | Genomic cassette (Synthetic) | Bacterial Expression, Synthetic Biology |
Ellis
| GeneGuard: a Modular Plasmid System Designed for Biosafety.
ACS Synth Biol. 2014 May 13.
|
| pSEVA12TEC-B | GeneGuard genomic cassette: for constructing ΔthyA::RBS B repA, Epsilon, FRT-kanR-FRT (low-copy support for ColE2 COR). Will support pSEVAT7Z. | Genomic cassette (Synthetic) | Bacterial Expression, Synthetic Biology |
Ellis
| GeneGuard: a Modular Plasmid System Designed for Biosafety.
ACS Synth Biol. 2014 May 13.
|
| pSEVA12TEC-I | GeneGuard genomic cassette: for constructing ΔthyA::RBS I repA, Epsilon, FRT-kanR-FRT (medium-copy support for ColE2 COR). Will support pSEVAT7Z. | Genomic cassette (Synthetic) | Bacterial Expression, Synthetic Biology |
Ellis
| GeneGuard: a Modular Plasmid System Designed for Biosafety.
ACS Synth Biol. 2014 May 13.
|
| p005kanRFP | RFP from coral Corynactis sp. under T5 promoter with lac operators; kanamycin resistant; to be used in practicalsyntheticbiology.net/resources | Red fluorescent protein (Other) | Bacterial Expression, Synthetic Biology |
Bryk
|
Practical synthetic biology
(unpublished)
|
| pSRKBB-empty | Contains BioBrick prefixed and suffixed insert with a modified lac promoter, Shine-Delgarno sequence, 5’ & 3’ MCS, 3’ 6xHis tag. | | Bacterial Expression |
Schmidt-dannert
|
BioBrickâ„¢ compatible plasmids for Rhodococcus
(unpublished)
|
| pCAP01 | The function of this material is to facilitate the capture and expression of bacterial gene clusters. | | Bacterial Expression, Yeast Expression, Synthetic Biology ; transformation-associated recombination cloning |
Moore
| Direct cloning and refactoring of a silent lipopeptide biosynthetic gene cluster yields the antibiotic taromycin A.
Proc Natl Acad Sci U S A. 2014 Feb 4;111(5):1957-62. doi: 10.1073/pnas.1319584111. Epub 2014 Jan 21.
|
| pBAM1 | This plasmids is a modular mini-Tn5 vector that could be used to generate random mutagenesis libraries or to insert heterologous DNA into a target genome | | Synthetic Biology |
de Lorenzo
| pBAM1: an all-synthetic genetic tool for analysis and construction of complex bacterial phenotypes.
BMC Microbiol. 2011 Feb 22;11:38. doi: 10.1186/1471-2180-11-38.
|
| pCAS | Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeast | S. pyogenese Cas9 (Other), RNA pol III promoter (tRNA-Tyr) (Saccharomyces cerevisiae), hepatitis delta virus ribozyme, genomic (Other), sgRNA (Synthetic) | Bacterial Expression, Yeast Expression, CRISPR, Synthetic Biology |
Cate
| Selection of chromosomal DNA libraries using a multiplex CRISPR system.
Elife. 2014 Aug 19;3. doi: 10.7554/eLife.03703.
|
| pAE03 | To construct C-terminal GFP fusions, single crossover integration vector | | Bacterial Expression, Synthetic Biology |
Veening
| Cellular localization of choline-utilization proteins in Streptococcus pneumoniae using novel fluorescent reporter systems.
Mol Microbiol. 2009 Oct;74(2):395-408. doi: 10.1111/j.1365-2958.2009.06872.x. Epub 2009 Sep 8.
|
| pJWV25 | To construct N-terminal GFP fusions under control of a Zn2+ inducible promoter, integrates via double crossover at bgaA | | Bacterial Expression, Synthetic Biology |
Veening
| Cellular localization of choline-utilization proteins in Streptococcus pneumoniae using novel fluorescent reporter systems.
Mol Microbiol. 2009 Oct;74(2):395-408. doi: 10.1111/j.1365-2958.2009.06872.x. Epub 2009 Sep 8.
|
| pPEP1 | BglFusion compatible integration vector for Streptococcus pneumoniae | | Bacterial Expression, Synthetic Biology |
Veening
| Gene Expression Platform for Synthetic Biology in the Human Pathogen Streptococcus pneumoniae.
ACS Synth Biol. 2014 Jun 4.
|
| pCM433kanT | Sucrose counterselection (sacB) vector for unmarked allelic exchange. | | Bacterial Expression, Synthetic Biology ; Sucrose counterselection |
Lidstrom
| Genetic tools for the industrially promising methanotroph Methylomicrobium buryatense.
Appl Environ Microbiol. 2014 Dec 29. pii: AEM.03795-14.
|
| pAWP78 | Small IncP-based broad host range replicating vector. | | Bacterial Expression, Synthetic Biology |
Lidstrom
| Genetic tools for the industrially promising methanotroph Methylomicrobium buryatense.
Appl Environ Microbiol. 2014 Dec 29. pii: AEM.03795-14.
|
| pAWP89 | IncP-based replicating vector expressing dTomato under tac promoter. | Ptac-dTomato | Bacterial Expression, Synthetic Biology |
Lidstrom
| Genetic tools for the industrially promising methanotroph Methylomicrobium buryatense.
Appl Environ Microbiol. 2014 Dec 29. pii: AEM.03795-14.
|
| Ai62(TITL-tdT) Flp-in replacement vector | Recombinase-mediated cassette exchange in ES cells to insert a Cre & tTA-dependent tdTomato expression cassette into a docking site within the mouse TIGRE genomic locus | chromatin insulator flanked TRE-LSL-tdTomato (Synthetic) | Recombinase-mediated cassette exchange using Flp recombinase |
Zeng
| Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance.
Neuron. 2015 Mar 4;85(5):942-58. doi: 10.1016/j.neuron.2015.02.022.
|
| pMU102 | Cloning and expression vector for Clostridium thermocellum | | Bacterial Expression, Synthetic Biology |
Lynd
| Computational design and characterization of a temperature-sensitive plasmid replicon for gram positive thermophiles.
J Biol Eng. 2012 May 11;6(1):5. doi: 10.1186/1754-1611-6-5.
|
| MXS_chaining vector | Empty Backbone | | Synthetic Biology |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
| MXS_MCS1 | MXS_chaining vector with MCS1 | EcoRV-MluI-XhoI-NheI-XbaI-NcoI-KpnI-BclI-BamHI-SalI MCS (Synthetic) | Synthetic Biology |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
| MXS_MCS2 | MXS_chaining vector with MCS2 | EcoRV-MluI-XhoI-EcoRI-BglII-KpnI-SalI MCS (Synthetic) | Synthetic Biology |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
| MXS_MCS3 | MXS_chaining vector with MCS3 | MluI-XhoI-HindIII-PstI-SalI MCS (Synthetic) | Synthetic Biology |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
| MXS_MCS4 | MXS_chaining vector with MCS4 | MluI-XhoI-PvuII-BglII-SalI MCS (Synthetic) | Synthetic Biology |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
| SMX DEST vector | Empty Backbone | | Synthetic Biology |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
| MXS_TagBFP | MXS_chaining vector with TagBFP | TagBFP (Synthetic) | Synthetic Biology |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
| MXS_Cerulean | MXS_chaining vector with Cerulean | Cerulean (Synthetic) | Synthetic Biology |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
| MXS_LSS-mKate2 | MXS_chaining vector with LSS-mKate2 | LSS-mKate2 (Synthetic) | Synthetic Biology |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
| MXS_mEGFP | MXS_chaining vector with mEGFP | mEGFP (Synthetic) | Synthetic Biology |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
| MXS_mAzamiGreen | MXS_chaining vector with mAzamiGreen | mAzamiGreen (Synthetic) | Synthetic Biology |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
| MXS_Citrine | MXS_chaining vector with Citrine | Citrine (Synthetic) | Synthetic Biology |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
| MXS_mKO2 | MXS_chaining vector with mKO2 | mKO2 (Synthetic) | Synthetic Biology |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
| MXS_tdTomato | MXS_chaining vector with tdTomato | tdTomato (Synthetic) | Synthetic Biology |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
| MXS_mCherry | MXS_chaining vector with mCherry | mCherry (Synthetic) | Synthetic Biology |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
| MXS_mPlum | MXS_chaining vector with mPlum | mPlum (Synthetic) | Synthetic Biology |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
| MXS_E2-Crimson | MXS_chaining vector with E2-Crimson | E2-Crimson (Synthetic) | Synthetic Biology |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
| MXS_iRFP670 | MXS_chaining vector with iRFP670 | iRFP670 (Synthetic) | Synthetic Biology |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
| MXS_PA-GFP | MXS_chaining vector with PA-GFP | PA-GFP (Synthetic) | Synthetic Biology |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
| MXS_Kaede | MXS_chaining vector with Kaede | Kaede (Synthetic) | Synthetic Biology |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
| MXS_H2B | MXS_chaining vector with H2B | Histone 2B with leading Kozak sequence (Synthetic) | Synthetic Biology |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
| MXS_P2A | MXS_chaining vector with P2A | Self-cleaving peptide (Synthetic) | Synthetic Biology |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
| MXS_CMV::HygroR-bGHpA | MXS_chaining vector with CMV::HygroR-bGHpA | resistance cassette against hygromycin B with CMV Promoter (Synthetic) | Synthetic Biology |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
| MXS_PGK::HygroR-bGHpA | MXS_chaining vector with PGK::HygroR-bGHpA | resistance cassette against hygromycin B with PGK Promoter (Synthetic) | Synthetic Biology |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
| MXS_CMV::NeoR-bGHpA | MXS_chaining vector with CMV::NeoR-bGHpA | resistance cassette against Neomycin with CMV Promoter (Synthetic) | Synthetic Biology |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
| MXS_PGK::NeoR-bGHpA | MXS_chaining vector with PGK::NeoR-bGHpA | resistance cassette against Neomycin with PGK Promoter (Synthetic) | Synthetic Biology |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
| MXS_CMV::PuroR-bGHpA | MXS_chaining vector with CMV::PuroR-bGHpA | resistance cassette against Puromycin with CMV Promoter (Synthetic) | Synthetic Biology |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
| MXS_PGK::PuroR-bGHpA | MXS_chaining vector with PGK::PuroR-bGHpA | resistance cassette against Puromycin with PGK Promoter (Synthetic) | Synthetic Biology |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
| MXS_CMV::ZeoR-bGHpA | MXS_chaining vector with CMV::ZeoR-bGHpA | resistance cassette against Zeocin with CMV Promoter (Synthetic) | Synthetic Biology |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
| MXS_PGK::ZeoR-bGHpA | MXS_chaining vector with PGK::ZeoR-bGHpA | resistance cassette against Zeocin with PGK Promoter (Synthetic) | Synthetic Biology |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
| ColorfulCell | MXS_chaining vector with 6 cassettes | CMV::GTS-Citrine-bGHpA (Synthetic), CMV::Lyn-Ceruleanx3-bGHpA (Synthetic), CMV::NLS-TagBFPx3-bGHpA (Synthetic), CMV::MTS-AzamiGreen-bGHpA (Synthetic), CMV::iRFP670-SKL-bGHpA (Synthetic), CMV::ETS-mCherry-KDEL-bGHpA (Synthetic) | Synthetic Biology |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
| ES-FUCCI | SMX_DEST vector with 3 cassettes | minPGK::Citrine-Geminin(1-110)-bGHpA (PGKEnhancer)x5 (Synthetic), minPGK::Cherry-hCdt1(30-120)-bGHpA (Synthetic), PGK::HygroR-bGpA (Synthetic) | Synthetic Biology |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
| rtTA and TagBFP cassettes | MXS_chaining vector with 2 cassettes | CMV::NLS-TagBFPx3-bGHpA (Synthetic), CMV::rtTA3-bGHpA (Synthetic) | Synthetic Biology |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
| EmptyTranscriptionalReporter | MXS_chaining vector with 2 cassettes | CMVmin::NLS-Citrine-bGHpA (Synthetic), CMV::NLS-Cherry-bGHpA (Synthetic) | Synthetic Biology |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
| pHL391 | Lim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + PLtetO-1::RBS (st7) hfq::mCherry::T1 terminator + ColE1 | gfp, mCherry | Bacterial Expression, Synthetic Biology |
Lim
| Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding.
Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
|
| pHL417 | Lim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + PLtetO-1::micC::Asp terminator + pLtetO-1::RBS (st7) hfq::T1 terminator + ColE1 | gfp, micC leader, hfq leader | Bacterial Expression, Synthetic Biology |
Lim
| Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding.
Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
|
| pHL518 | Lim Lab plasmid: AmpR + PLlacO-1::micC::mCherry::T1 terminator + PLtetO-1m9::OmpC::gfp::T1T2 terminator + ColE1 | mCherry, gfp | Bacterial Expression, Synthetic Biology |
Lim
| Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding.
Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
|
| pHL671 | Lim Lab plasmid: AmpR + PCon::glgC::gfp::T1T2 terminator + ColE1 | gfp | Bacterial Expression, Synthetic Biology |
Lim
| Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding.
Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
|
| pHL718 | Lim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + PLtetO-1::ipex::mCherry::T1 terminator + ColE1 | gfp, mCherry | Bacterial Expression, Synthetic Biology |
Lim
| Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding.
Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
|
| pHL836 | Lim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + PLtetO-1::ipex::Asp terminator + PLtetO-1::RBS (st7) hfq::T1 terminator + ColE1 | gfp, ipex leader, hfq leader | Bacterial Expression, Synthetic Biology |
Lim
| Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding.
Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
|
| pHL1017 | Lim Lab plasmid: AmpR + PConNoHindM8::glgC::gfp::T1T2 terminator + ColE1 | gfp | Bacterial Expression, Synthetic Biology |
Lim
| Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding.
Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
|
| pHL1632 | Lim Lab plasmid: AmpR + PLlacO-1::RBS(st7) gfp::Asp terminator + ColE1 | gfp | Bacterial Expression, Synthetic Biology |
Lim
| Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding.
Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
|
| pHL1633 | Lim Lab plasmid: AmpR + PLlacO-1::T710 gfp::Asp terminator + ColE1 | gfp | Bacterial Expression, Synthetic Biology |
Lim
| Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding.
Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
|
| pHL1954 | Lim Lab plasmid: AmpR + PconNoHindM8::ryhB::Asp terminator + PLlacO-1::RBS (st7) hfq::T1 terminator + PLtetO-1m9::sodB::gfp::T1T2 terminator + ColE1 | M8::ryhB leader, hfq leader, gfp | Bacterial Expression, Synthetic Biology |
Lim
| Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding.
Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
|
| pHL1969 | Lim Lab plasmid: AmpR + PconNoHindM8::ryhB::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::sodB::gfp::T1T2 terminator + ColE1 | M8::ryhB leader, T710 hfq leader, gfp | Bacterial Expression, Synthetic Biology |
Lim
| Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding.
Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
|
| pHL2021 | Lim Lab plasmid: AmpR + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::sodB::gfp::T1T2 terminator + ColE1 | T710 hfq leader, gfp | Bacterial Expression, Synthetic Biology |
Lim
| Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding.
Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
|
| pHL2026 | Lim Lab plasmid: AmpR + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::glmS::gfp::T1T2 terminator + ColE1 | T710 hfq leader, gfp | Bacterial Expression, Synthetic Biology |
Lim
| Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding.
Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
|
| pHL2027 | Lim Lab plasmid: AmpR + PLlacO-1::T710 hfq::T1terminator + PLtetO-1::glmS::gfp::T1T2 terminator + ColE1 | T710 hfq leader, gfp | Bacterial Expression, Synthetic Biology |
Lim
| Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding.
Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
|
| pHL2053 | Lim Lab plasmid: AmpR + PLlacO-1::T710::hfq::T1 termintor + PLtetO-1m9::ompC::gfp::T1T2 terminator + ColE1 | T710 hfq leader, gfp | Bacterial Expression, Synthetic Biology |
Lim
| Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding.
Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
|
| pHL2073 | Lim Lab plasmid: AmpR + PconShortNoHind::glmZ::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::glmS::gfp::T1T2 terminator + ColE1 | glmZ leader, T710 hfq leader, gfp | Bacterial Expression, Synthetic Biology |
Lim
| Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding.
Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
|
| pHL2075 | Lim Lab plasmid: AmpR + PconShortNoHind::glmZ::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1::glmS::gfp::T1T2 terminator + ColE1 | glmZ leader, T710 hfq leader, gfp | Bacterial Expression, Synthetic Biology |
Lim
| Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding.
Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
|
| pHL2084 | Lim Lab plasmid: AmpR + PconShortNoHindM8::glmZ::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::glmS::gfp::T1T2 terminator + ColE1 | m8::glmZ leader, T710 hfq leader, gfp | Bacterial Expression, Synthetic Biology |
Lim
| Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding.
Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
|
| pHL2085 | Lim Lab plasmid: AmpR + PconShortNoHindM8::glmZ::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1::glmS::gfp::T1T2 terminator + ColE1 | m8::glmZ leader, T710 hfq leader, gfp | Bacterial Expression, Synthetic Biology |
Lim
| Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding.
Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
|
| pHL2107 | Lim Lab plasmid: AmpR + PconNoHindM8::micC::Asp terminator + PLlacO-1::T710::hfq::T1 terminator + PLtetO-1m9::ompC::gfp::T1T2 terminator + ColE1 | m8::micC leader, T710 hfq leader, gfp | Bacterial Expression, Synthetic Biology |
Lim
| Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding.
Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
|
| pHL2117 | Lim Lab plasmid: AmpR + PconNoHindM8::ryhB::Asp terminator + PLtetO-1m9::sodB::gfp::T1T2 terminator + ColE1 | M8::ryhB leader, gfp | Bacterial Expression, Synthetic Biology |
Lim
| Paradoxical suppression of small RNA activity at high Hfq concentrations due to random-order binding.
Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
|
| pReporter_13_switchBP | A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp). attP upstream of attB in this plasmid. | Reporter_13_switchBP (Synthetic) | Bacterial Expression |
Voigt
| Permanent genetic memory with >1-byte capacity.
Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
|
| pAV113 | pRS413 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. | | Bacterial Expression, Yeast Expression, Synthetic Biology |
Boeke
| Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units.
ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
|
| pAV114 | pRS414 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. | | Bacterial Expression, Yeast Expression, Synthetic Biology |
Boeke
| Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units.
ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
|
| pAV115 | pRS415 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. | | Bacterial Expression, Yeast Expression, Synthetic Biology |
Boeke
| Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units.
ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
|
| pAV116 | pRS416 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. | | Bacterial Expression, Yeast Expression, Synthetic Biology |
Boeke
| Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units.
ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
|
| pAV11K | pRS416 backbone, URA3 replaced by KanMX, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. | | Bacterial Expression, Yeast Expression, Synthetic Biology |
Boeke
| Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units.
ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
|
| pAV113.loxP | pRS413 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites. | | Bacterial Expression, Yeast Expression, Synthetic Biology |
Boeke
| Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units.
ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
|
| pAV115.loxP | pRS415 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites. | | Bacterial Expression, Yeast Expression, Synthetic Biology |
Boeke
| Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units.
ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
|
| pAV116.loxP | pRS416 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites. | | Bacterial Expression, Yeast Expression, Synthetic Biology |
Boeke
| Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units.
ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
|
| pAV123 | pRS423 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. | | Bacterial Expression, Yeast Expression, Synthetic Biology |
Boeke
| Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units.
ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
|
| pAV124 | pRS424 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. | | Bacterial Expression, Yeast Expression, Synthetic Biology |
Boeke
| Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units.
ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
|
| pAV125 | pRS425 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. | | Bacterial Expression, Yeast Expression, Synthetic Biology |
Boeke
| Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units.
ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
|
| pAV126 | pRS426 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. | | Bacterial Expression, Yeast Expression, Synthetic Biology |
Boeke
| Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units.
ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
|
| pAV103 | pRS403 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. | | Yeast Expression, Worm Expression, Synthetic Biology |
Boeke
| Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units.
ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
|
| pAV104 | pRS404 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. | | Bacterial Expression, Yeast Expression, Synthetic Biology |
Boeke
| Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units.
ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
|
| pAV105 | pRS405 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. | | Bacterial Expression, Yeast Expression, Synthetic Biology |
Boeke
| Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units.
ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
|
| pAV106 | pRS406 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. | | Bacterial Expression, Yeast Expression, Synthetic Biology |
Boeke
| Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units.
ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
|
| pAV10.F3 | BioBrick pSB1C3 backbone (Cm), HIS3, RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. Integration into chrVI: 97873-98803 (NotI or BciVI). | | Bacterial Expression, Yeast Expression, Synthetic Biology |
Boeke
| Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units.
ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
|
| pAV10.F3.loxP | pCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into chrVI: 97873-98803 (NotI or BciVI). | | Bacterial Expression, Yeast Expression, Synthetic Biology |
Boeke
| Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units.
ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
|
| pAV10.F6.loxP | pCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into chrVI: 97873-98803 (NotI or BciVI). | | Bacterial Expression, Yeast Expression, Synthetic Biology |
Boeke
| Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units.
ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
|
| pAV10.K3.loxP | pCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI). | | Bacterial Expression, Yeast Expression, Synthetic Biology |
Boeke
| Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units.
ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
|
| pAV10.K6.loxP | pCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI or BciVI). | | Bacterial Expression, Yeast Expression, Synthetic Biology |
Boeke
| Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units.
ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
|
| pAV10.K5.loxP | pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI or BciVI). | | Bacterial Expression, Yeast Expression, Synthetic Biology |
Boeke
| Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units.
ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
|
| pAV10.HO3.loxP | pCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into HO locus (NotI). | | Bacterial Expression, Yeast Expression, Synthetic Biology |
Boeke
| Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units.
ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
|
| pAV10.HO5.loxP | pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into HO locus (NotI or BciVI). | | Bacterial Expression, Yeast Expression, Synthetic Biology |
Boeke
| Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units.
ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
|
| pAV10.KH | pCACS backbone (Amp), Hyg, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI). | | Bacterial Expression, Yeast Expression, Synthetic Biology |
Boeke
| Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units.
ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
|
| pAV10.KN | pCACS backbone (Amp), cloNAT, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI). | | Bacterial Expression, Yeast Expression, Synthetic Biology |
Boeke
| Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units.
ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
|
| pAV10.K5 | pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI or BciVI). | | Bacterial Expression, Yeast Expression, Synthetic Biology |
Boeke
| Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units.
ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
|
| pAV10.HO6 | pCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into HO locus (NotI or BciVI). | | Bacterial Expression, Yeast Expression, Synthetic Biology |
Boeke
| Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units.
ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
|
| pAV10.K4 | pCACS backbone (Amp), TRP1, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into chrIXR: 387328-388330. | | Bacterial Expression, Yeast Expression, Synthetic Biology |
Boeke
| Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units.
ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
|
| pAV10 | pUC19 backbone (Amp), contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. NotI or FseI sites for TU digest. | | Bacterial Expression, Synthetic Biology |
Boeke
| Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units.
ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
|
| pUAP1 | Accepts DNA sequences via BpiI cloning sites, resulting in Level 0 standard parts for Golden Gate cloning. Parts can be released with a user-defined 4-base-pair, 5 prime overhangs using BsaI. | RFP cloning selection cassette (Synthetic) | Synthetic Biology |
Patron
| Standards for plant synthetic biology: a common syntax for exchange of DNA parts.
New Phytol. 2015 Jul 14. doi: 10.1111/nph.13532.
|
| pEERM1 Km | Plasmid for integration into psbA2 site of Synechocystis 6803 | | Bacterial Expression, Synthetic Biology |
Lindberg
| Metabolic Engineering of Synechocystis sp. PCC 6803 for Production of the Plant Diterpenoid Manoyl Oxide.
ACS Synth Biol. 2015 Jul 13.
|
| pEERM3 Km | Plasmid for integration into neutral site of Synechocystis 6803 | | Bacterial Expression, Synthetic Biology |
Lindberg
| Metabolic Engineering of Synechocystis sp. PCC 6803 for Production of the Plant Diterpenoid Manoyl Oxide.
ACS Synth Biol. 2015 Jul 13.
|
| pEERM4 Cm | Plasmid for integration into neutral site 2 of Synechocystis 6803 | | Bacterial Expression, Synthetic Biology |
Lindberg
| Metabolic Engineering of Synechocystis sp. PCC 6803 for Production of the Plant Diterpenoid Manoyl Oxide.
ACS Synth Biol. 2015 Jul 13.
|
| pEERM6 Km | Plasmid for integration into sqs site of Synechocystis 6803 | | Bacterial Expression, Synthetic Biology |
Lindberg
| Metabolic Engineering of Synechocystis sp. PCC 6803 for Production of the Plant Diterpenoid Manoyl Oxide.
ACS Synth Biol. 2015 Jul 13.
|
| pCAPB02 | A transformation-associated recombination (TAR) based genomic capture and expression vector for antibiotic production in B. subtilis | | Bacterial Expression, Synthetic Biology |
Qian
| Directed natural product biosynthesis gene cluster capture and expression in the model bacterium Bacillus subtilis.
Sci Rep. 2015 Mar 24;5:9383. doi: 10.1038/srep09383.
|
| J23100_AB | MoClo Basic Part: Constitutive promoter - Anderson series - high strength [A:J23100:B] | Constitutive promoter (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| J23100_EB | MoClo Basic Part: Constitutive promoter - Anderson series - high strength [E:J23100:B] | Constitutive promoter (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| J23100_FB | MoClo Basic Part: Constitutive promoter - Anderson series - high strength [F:J23100:B] | Constitutive promoter (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| J23100_GB | MoClo Basic Part: Constitutive promoter - Anderson series - high strength [G:J23100:B] | Constitutive promoter (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| J23102_AB | MoClo Basic Part: Constitutive promoter - Anderson series - high strength [A:J23102:B] | Constitutive promoter (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| J23102_EB | MoClo Basic Part: Constitutive promoter - Anderson series - high strength [E:J23102:B] | Constitutive promoter (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| J23102_FB | MoClo Basic Part: Constitutive promoter - Anderson series - high strength [F:J23102:B] | Constitutive promoter (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| J23102_GB | MoClo Basic Part: Constitutive promoter - Anderson series - high strength [G:J23102:B] | Constitutive promoter (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| J23103_AB | MoClo Basic Part: Constitutive promoter - Anderson series - low strength [A:J23103:B] | Constitutive promoter (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| J23103_EB | MoClo Basic Part: Constitutive promoter - Anderson series - low strength [E:J23103:B] | Constitutive promoter (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| J23103_FB | MoClo Basic Part: Constitutive promoter - Anderson series - low strength [F:J23103:B] | Constitutive promoter (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| J23103_GB | MoClo Basic Part: Constitutive promoter - Anderson series - low strength [G:J23103:B] | Constitutive promoter (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| J23106_AB | MoClo Basic Part: Constitutive promoter - Anderson series - medium strength [A:J23106:B] | Constitutive promoter (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| J23106_EB | MoClo Basic Part: Constitutive promoter - Anderson series - medium strength [E:J23106:B] | Constitutive promoter (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| J23106_FB | MoClo Basic Part: Constitutive promoter - Anderson series - medium strength [F:J23106:B] | Constitutive promoter (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| J23106_GB | MoClo Basic Part: Constitutive promoter - Anderson series - medium strength [G:J23106:B] | Constitutive promoter (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| J23107_AB | MoClo Basic Part: Constitutive promoter - Anderson series - medium strength [A:J23107:B] | Constitutive promoter (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| J23107_EB | MoClo Basic Part: Constitutive promoter - Anderson series - medium strength [E:J23107:B] | Constitutive promoter (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| J23107_FB | MoClo Basic Part: Constitutive promoter - Anderson series - medium strength [F:J23107:B] | Constitutive promoter (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| J23107_GB | MoClo Basic Part: Constitutive promoter - Anderson series - medium strength [G:J23107:B] | Constitutive promoter (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| J23116_AB | MoClo Basic Part: Constitutive promoter - Anderson series - low strength [A:J23116:B] | Constitutive promoter (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| J23116_EB | MoClo Basic Part: Constitutive promoter - Anderson series - low strength [E:J23116:B] | Constitutive promoter (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| J23116_GB | MoClo Basic Part: Constitutive promoter - Anderson series - low strength [G:J23116:B] | Constitutive promoter (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| R0010 (pLacI)_AB | MoClo Basic Part: Controllable promoter - pLacI - lacI regulated (repressed by LacI+CAP. LacI, C0012, inhibited by IPTG) [A:R0010:B] | Controllable promoter (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| R0010 (pLacI)_EB | MoClo Basic Part: Controllable promoter - pLacI - lacI regulated (repressed by LacI+CAP. LacI, C0012, inhibited by IPTG) [E:R0010:B] | Controllable promoter (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| R0010 (pLacI)_FB | MoClo Basic Part: Controllable promoter - pLacI - lacI regulated (repressed by LacI+CAP. LacI, C0012, inhibited by IPTG) [F:R0010:B] | Controllable promoter (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| R0010 (pLacI)_GB | MoClo Basic Part: Controllable promoter - pLacI - lacI regulated (repressed by LacI+CAP. LacI, C0012, inhibited by IPTG) [G:R0010:B] | Controllable promoter (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| R0040 (pTet)_AB | MoClo Basic Part: Controllable promoter - pTet - tetR regulated (strong constitutive promoter repressed by tetR, C0040, which can itself be inhibited by tetracycline or aTc) [A:R0040:B] | Controllable promoter (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| R0040 (pTet)_EB | MoClo Basic Part: Controllable promoter - pTet - tetR regulated (strong constitutive promoter repressed by tetR, C0040, which can itself be inhibited by tetracycline or aTc) [E:R0040:B] | Controllable promoter (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| R0040 (pTet)_FB | MoClo Basic Part: Controllable promoter - pTet - tetR regulated (strong constitutive promoter repressed by tetR, C0040, which can itself be inhibited by tetracycline or aTc) [F:R0040:B] | Controllable promoter (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| R0040 (pTet)_GB | MoClo Basic Part: Controllable promoter - pTet - tetR regulated (strong constitutive promoter repressed by tetR, C0040, which can itself be inhibited by tetracycline or aTc) [G:R0040:B] | Controllable promoter (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| R0063 (pLuxR-pR)_AB | MoClo Basic Part: Controllable promoter - pLuxR(pR) - luxR regulated (right promoter, weak constitutive down regulated by LuxR, C0062 in combination with homoserine lactone (HSL)) [A:R0063:B] | Controllable promoter (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| R0063 (pLuxR-pR)_EB | MoClo Basic Part: Controllable promoter - pLuxR(pR) - luxR regulated (right promoter, weak constitutive down regulated by LuxR, C0062 in combination with homoserine lactone (HSL)) [E:R0063:B] | Controllable promoter (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| R0063 (pLuxR-pR)_FB | MoClo Basic Part: Controllable promoter - pLuxR(pR) - luxR regulated (right promoter, weak constitutive down regulated by LuxR, C0062 in combination with homoserine lactone (HSL)) [F:R0063:B] | Controllable promoter (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| R0063 (pLuxR-pR)_GB | MoClo Basic Part: Controllable promoter - pLuxR(pR) - luxR regulated (right promoter, weak constitutive down regulated by LuxR, C0062 in combination with homoserine lactone (HSL)) [G:R0063:B] | Controllable promoter (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| I13453 (pBAD)_AB | MoClo Basic Part: Controllable promoter - pBAD - araC regulated (pBAD promoter, inducible by expression of AraC, C0080) [A:I13453:B] | Controllable promoter (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| I13453 (pBAD)_EB | MoClo Basic Part: Controllable promoter - pBAD - araC regulated (pBAD promoter, inducible by expression of AraC, C0080) [E:I13453:B] | Controllable promoter (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| I13453 (pBAD)_FB | MoClo Basic Part: Controllable promoter - pBAD - araC regulated (pBAD promoter, inducible by expression of AraC, C0080) [F:I13453:B] | Controllable promoter (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| I13453 (pBAD)_GB | MoClo Basic Part: Controllable promoter - pBAD - araC regulated (pBAD promoter, inducible by expression of AraC, C0080) [G:I13453:B] | Controllable promoter (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| B0032m_BC | MoClo Basic Part: RBS - Weiss RBS, medium strength. Modified from Bba_B0032 to adjust spacing in MC system. [B:B0032m:C] | RBS (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| B0033m_BC | MoClo Basic Part: RBS - Weiss RBS, low strength. Modified from Bba_B0033 to adjust spacing in MC system. [B:B0033m:C] | RBS (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| B0034m_BC | MoClo Basic Part: RBS - Weiss RBS, high strength. Modified from Bba_B0034 to adjust spacing in MC system. [B:B0034m:C] | RBS (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| BCD12_BC | MoClo Basic Part: RBS - BiCistronic Design, medium strength (RBS part type, actually contains a small transcriptional unit and second RBS) [B:BCD12:C] | RBS (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| BCD2_BC | MoClo Basic Part: RBS - BiCistronic Design, high strength (RBS part type, actually contains a small transcriptional unit and second RBS) [B:BCD2:C] | RBS (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| BCD8_BC | MoClo Basic Part: RBS - BiCistronic Design, low strength (RBS part type, actually contains a small transcriptional unit and second RBS) [B:BCD8:C] | RBS (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| C0012m (lacI)_CD | MoClo Basic Part: CDS - Controller protein, lacI repressor (in concert with CAP, represses pLacI, R0010). Modified to fix illegal site. [C:C0012m:D] | Transcription factor (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| C0040 (tetR)_CD | MoClo Basic Part: CDS - Controller protein, tetR repressor (represses pTet, C0040. can be inhibited by tetracyclin or aTc) [C:C0040:D] | Transcription factor (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| C0062 (luxR)_CD | MoClo Basic Part: CDS - Controller protein, luxR repressor/activator (in concert with HSL, represses pLuxR(pR) R0063. Also up-regulates pLuxR(pL) R0062) [C:C0062:D] | Transcription factor (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| C0080 (araC)_CD | MoClo Basic Part: CDS - Controller protein, araC repressor/activator (activates pBAD, I13453) [C:C0080:D] | Transcription factor (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| cre_CD | MoClo Basic Part: CDS - cre recombinase (recombines DNA at loxP sites) [C:cre:D] | Recombinase (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| E0030 (YFP)_CD | MoClo Basic Part: CDS - Fluorescent protein. Yellow. [C:E0030:D] | Fluorescent reporter - YFP (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| E0040m (GFP)_CD | MoClo Basic Part: CDS - Fluorescent protein. Green. Modified from Bba_E0040 to fix illegal site. [C:E0040m:D] | Fluorescent reporter - GFP (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| E1010m (RFP)_CD | MoClo Basic Part: CDS - Fluorescent protein. Red. Modified from Bba_E1010 to fix illegal sites. [C:E1010m:D] | Fluorescent reporter - RFP (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| eBFP2 (eBFP2)_CD | MoClo Basic Part: CDS - Fluorescent protein. Blue. Derived from Addgene#14891. [C:eBFP2:D] | Fluorescent reporter - BFP (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| B0015_DE | MoClo Basic Part: Double terminator (B0010:B0012) [D:B0015:E] | Double Terminator (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| B0015_DF | MoClo Basic Part: Double terminator (B0010:B0012) [D:B0015:F] | Double Terminator (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| B0015_DG | MoClo Basic Part: Double terminator (B0010:B0012) [D:B0015:G] | Double Terminator (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| B0015_DH | MoClo Basic Part: Double terminator (B0010:B0012) [D:B0015:H] | Double Terminator (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| DVA_AB | MoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [A:LacZa:B] | None (Synthetic) | Synthetic Biology ; Destination Vector |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| DVA_AE | MoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [A:LacZa:E] | None (Synthetic) | Synthetic Biology ; Destination Vector |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| DVA_AF | MoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [A:LacZa:F] | None (Synthetic) | Synthetic Biology ; Destination Vector |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| DVA_AG | MoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [A:LacZa:G] | None (Synthetic) | Synthetic Biology ; Destination Vector |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| DVA_AH | MoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [A:LacZa:H] | None (Synthetic) | Synthetic Biology ; Destination Vector |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| DVA_BC | MoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [B:LacZa:C] | None (Synthetic) | Synthetic Biology ; Destination Vector |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| DVA_CD | MoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [C:LacZa:D] | None (Synthetic) | Synthetic Biology ; Destination Vector |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| DVA_DE | MoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [D:LacZa:E] | None (Synthetic) | Synthetic Biology ; Destination Vector |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| DVA_DF | MoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [D:LacZa:F] | None (Synthetic) | Synthetic Biology ; Destination Vector |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| DVA_DG | MoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [D:LacZa:G] | None (Synthetic) | Synthetic Biology ; Destination Vector |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| DVA_DH | MoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [D:LacZa:H] | None (Synthetic) | Synthetic Biology ; Destination Vector |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| DVA_EB | MoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [E:LacZa:B] | None (Synthetic) | Synthetic Biology ; Destination Vector |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| DVA_EF | MoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [E:LacZa:F] | None (Synthetic) | Synthetic Biology ; Destination Vector |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| DVA_EG | MoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [E:LacZa:G] | None (Synthetic) | Synthetic Biology ; Destination Vector |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| DVA_EH | MoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [E:LacZa:H] | None (Synthetic) | Synthetic Biology ; Destination Vector |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| DVA_FB | MoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [F:LacZa:B] | None (Synthetic) | Synthetic Biology ; Destination Vector |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| DVA_GB | MoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [G:LacZa:B] | None (Synthetic) | Synthetic Biology ; Destination Vector |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| DVA | MoClo Destination Vector: Empty vector for creating new DVA. Cut with SpeI and insert amplified LacZa (based on pSB1A2) | None (Synthetic) | Synthetic Biology ; Destination Vector |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| pJ02B2Gm_AE | MoClo Transcriptional Unit: FACS Standard Color Controls - High GFP expression cassette - Ampicillin plasmid [A:J23102:B:BCD2:C:E0040m:D:B0015:E] | FACS Controls (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| pJ02B2Gm_EF | MoClo Transcriptional Unit: FACS Standard Color Controls - High GFP expression cassette - Ampicillin plasmid [E:J23102:B:BCD2:C:E0040m:D:B0015:F] | FACS Controls (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| pJ02B2Rm_AE | MoClo Transcriptional Unit: FACS Standard Color Controls - High RFP expression cassette - Ampicillin plasmid [A:J23102:B:BCD2:C:E1010m:D:B0015:E] | FACS Controls (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| pJ02B2Rm_EF | MoClo Transcriptional Unit: FACS Standard Color Controls - High RFP expression cassette - Ampicillin plasmid [E:J23102:B:BCD2:C:E1010m:D:B0015:F] | FACS Controls (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| pJ02B2Gm:Rm_AF | MoClo Device: FACS Standard Color Controls - High GFP (first unit) & RFP (second unit) expression cassette - Ampicillin plasmid [A:J23102:B:BCD2:C:E0040m:D:B0015:E:J23102:B:BCD2:C:E1010m:D:B0015:F] | FACS Controls (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| pJ02B2Rm:Gm_AF | MoClo Device: FACS Standard Color Controls - High RFP (first unit) & GFP (second unit) expression cassette - Ampicillin plasmid [A:J23102:B:BCD2:C:E1010m:D:B0015:E:J23102:B:BCD2:C:E0040m:D:B0015:F] | FACS Controls (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| pJ02B2Gm_AE | MoClo Transcriptional Unit: FACS Standard Color Controls - High GFP expression cassette - Kanamycin plasmid [A:J23102:B:BCD2:C:E0040m:D:B0015:E] | FACS Controls (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| pJ02B2Gm_EF | MoClo Transcriptional Unit: FACS Standard Color Controls - High GFP expression cassette - Kanamycin plasmid [E:J23102:B:BCD2:C:E0040m:D:B0015:F] | FACS Controls (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| pJ02B2Rm_AE | MoClo Transcriptional Unit: FACS Standard Color Controls - High RFP expression cassette - Kanamycin plasmid [A:J23102:B:BCD2:C:E1010m:D:B0015:E] | FACS Controls (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| pJ02B2Rm_EF | MoClo Transcriptional Unit: FACS Standard Color Controls - High RFP expression cassette - Kanamycin plasmid [E:J23102:B:BCD2:C:E1010m:D:B0015:F] | FACS Controls (Synthetic) | Synthetic Biology |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| DVK_AE | MoClo Destination Vector: (based on pSB1K3) [A:LacZa:E] | None (Synthetic) | Synthetic Biology ; Destination Vector |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| DVK_AF | MoClo Destination Vector: (based on pSB1K3) [A:LacZa:F] | None (Synthetic) | Synthetic Biology ; Destination Vector |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| DVK_EF | MoClo Destination Vector: (based on pSB1K3) [E:LacZa:F] | None (Synthetic) | Synthetic Biology ; Destination Vector |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| DVK_FG | MoClo Destination Vector: (based on pSB1K3) [F:LacZa:G] | None (Synthetic) | Synthetic Biology ; Destination Vector |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| DVK_GH | MoClo Destination Vector: (based on pSB1K3) [G:LacZa:H] | None (Synthetic) | Synthetic Biology ; Destination Vector |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| DVK | MoClo Destination Vector: Empty vector for creating new DVK. Cut with SpeI and insert amplified LacZa (based on pSB1K3) | None (Synthetic) | Synthetic Biology ; Destination Vector |
Densmore
| CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology.
ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
|
| pShuttle | Mobilizible plasmid with EGFP. OriT from the F plasmid. | OriT (Other) | Synthetic Biology |
Leonard
|
Leonard Lab Conjugation plasmids
(unpublished)
|
| pCasper4-g-osk-TRICK | oskar translation biosensor with 12x copies of modified PP7 stem loops and 6xMS2 stem loops. . full genomic region, used for P element-mediated germline transformation in Drosophila. | oskar (Drosophila melanogaster) | Insect Expression ; P element-mediated germline transformation |
Ephrussi
| Translation. An RNA biosensor for imaging the first round of translation from single cells to living animals.
Science. 2015 Mar 20;347(6228):1367-671. doi: 10.1126/science.aaa3380.
|
| pTRK669 | Helper plasmid for pORI plasmids. Chloramphenicol resistant, temperature sensitive replication in E. coli and gram positives. Encodes RepA. | | Bacterial Expression ; E. coli-gram positive bacteria shuttle vector |
Klaenhammer
| Efficient system for directed integration into the Lactobacillus acidophilus and Lactobacillus gasseri chromosomes via homologous recombination.
Appl Environ Microbiol. 2001 Sep;67(9):4361-4.
|
| pTKDP-neo | Donor plasmid - kanamycin resistant donor cassette | | Bacterial Expression, Synthetic Biology |
Kuhlman
| An Integrated System for Precise Genome Modification in Escherichia coli.
PLoS One. 2015 Sep 2;10(9):e0136963. doi: 10.1371/journal.pone.0136963. eCollection 2015.
|
| pTKDP-cat | Donor plasmid - chloramphenicol resistant donor cassette | | Bacterial Expression, Synthetic Biology |
Kuhlman
| An Integrated System for Precise Genome Modification in Escherichia coli.
PLoS One. 2015 Sep 2;10(9):e0136963. doi: 10.1371/journal.pone.0136963. eCollection 2015.
|
| pTKDP-hph | Donor plasmid - hygromycin resistant donor cassette | | Bacterial Expression, Synthetic Biology |
Kuhlman
| An Integrated System for Precise Genome Modification in Escherichia coli.
PLoS One. 2015 Sep 2;10(9):e0136963. doi: 10.1371/journal.pone.0136963. eCollection 2015.
|
| pTKDP-dhfr | Donor plasmid - trimethoprim resistant donor cassette | | Bacterial Expression, Synthetic Biology |
Kuhlman
| An Integrated System for Precise Genome Modification in Escherichia coli.
PLoS One. 2015 Sep 2;10(9):e0136963. doi: 10.1371/journal.pone.0136963. eCollection 2015.
|
| pTKLP-tetA | tetA landing pad template plasmid | | Bacterial Expression, Synthetic Biology |
Kuhlman
| An Integrated System for Precise Genome Modification in Escherichia coli.
PLoS One. 2015 Sep 2;10(9):e0136963. doi: 10.1371/journal.pone.0136963. eCollection 2015.
|
| pSS9 | Contains 600 bp homology arms to SS9 for one-step markerless genome integration in E. coli | gfpuv (Synthetic) | Synthetic Biology |
Gill
| Rapid and Efficient One-Step Metabolic Pathway Integration in E. coli.
ACS Synth Biol. 2016 Apr 22.
|
| pAWp7-2 | pFUGW-UBCp-RFP-CMVp-GFP-[miR-128] | UBCp-RFP-CMVp-GFP-[miR-128] | Lentiviral |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
| pAWp7-14 | pFUGW-UBCp-RFP-[miR-128 sensor]-CMVp-GFP-[miR-128] | UBCp-RFP-[miR-128 sensor]-CMVp-GFP-[miR-128] | Lentiviral |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
| pAWSV-2 | pBT264-[let-7b/miR-4763 cluster] | [let-7b/miR-4763 cluster] | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
| pAWSV-3 | pBT264-[let-7c] | [let-7c] | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
| pAWSV-5 | pBT264-[let-7e/miR-99b cluster] | [let-7e/miR-99b cluster] | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
| pAWSV-7 | pBT264-[miR-10b] | [miR-10b] | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
| pAWSV-8 | pBT264-[miR-126] | [miR-126] | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
| pAWSV-9 | pBT264-[miR-128b] | [miR-128b] | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
| pAWSV-10 | pBT264-[miR-129-2] | [miR-129-2] | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
| pAWSV-11 | pBT264-[miR-132] | [miR-132] | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
| pAWSV-13 | pBT264-[miR-16-1/15a cluster] | [miR-16-1/15a cluster] | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
| pAWSV-14 | pBT264-[miR-181a] | [miR-181a] | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
| pAWSV-15 | pBT264-[miR-181c] | [miR-181c] | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
| pAWSV-16 | pBT264-[miR-188] | [miR-188] | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
| pAWSV-18 | pBT264-[miR-199b/3154 cluster] | [miR-199b/3154 cluster] | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
| pAWSV-19 | pBT264-[miR-211] | [miR-211] | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
| pAWSV-20 | pBT264-[miR-212] | [miR-212] | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
| pAWSV-21 | pBT264-[miR-216] | [miR-216] | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
| pAWSV-24 | pBT264-[miR-29b] | [miR-29b] | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
| pAWSV-26 | pBT264-[miR-31] | [miR-31] | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
| pAWSV-28 | pBT264-[miR-328] | [miR-328] | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
| pAWSV-29 | pBT264-[miR-34a] | [miR-34a] | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
| pAWSV-31 | pBT264-[miR-376a] | [miR-376a] | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
| pAWSV-32 | pBT264-[miR-429] | [miR-429] | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
| pAWSV-33 | pBT264-[miR-451/144/4732 cluster] | [miR-451/144/4732 cluster] | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
| pAWSV-34 | pBT264-[miR-488] | [miR-488] | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
| pAWSV-37 | pBT264-[miR-9-1] | [miR-9-1] | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
| pAWSV-38 | pBT264-[miR-93/106b cluster] | [miR-93/106b cluster] | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
| pTU1 | Backbone vector with internal BsaI removed by site-directed mutagenesis | | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pTU1-A-lacZ | Level 1 Destination vector - Position A | Level 1 - A site (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pTU1-B-lacZ | Level 1 Destination vector - Position B | Level 1 - B site (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pTU1-C-lacZ | Level 1 Destination vector - Position C | Level 1 - C site (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pTU1-D-lacZ | Level 1 Destination vector - Position D | Level 1 - D site (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pTU1-A-RFP | Level 1 Destination vector - Position A | Level 1 - A site (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pTU1-B-RFP | Level 1 Destination vector - Position B | Level 1 - B site (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pTU1-C-RFP | Level 1 Destination vector - Position C | Level 1 - C site (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pTU1-D-RFP | Level 1 Destination vector - Position D | Level 1 - D site (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pTU1-D1-RFP | Level 1 Destination vector - Position D1 | Level 1 - D1 site (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pTU1-E-RFP | Level 1 Destination vector - Position E | Level 1 - E site (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pTU3-A-RFP | Level 3 Destination vector - Assembly of 2 Level 2 modules - AB | Level 3 - A site (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pTU3-B-RFP | Level 3 Destination vector - Assembly of 4 Level 2 modules - ABCD | Level 3 - B site (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP | Backbone vector with internal BsmBI removed by site-directed mutagenesis | | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP-lacZ | Entry vector for Bioparts - Oligo anneal or PCR with internal BsaI sites at 5' and 3' ends using NdeI/SphI cloning | Level 0 - Bioparts (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP-ORF | Entry vector for ORF's - PCR, gene synthesis or sub-clone using NdeI/BamHI. Alternatively use BsaI with CATA and TCGA fusion sites. Stop codon must be included upstream of chosen 3' restriction site | Level 0 - ORFs (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pTU2-A-lacZ | Level 2 Destination vector - Position A - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE | Level 2 - A site (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pTU2-B-lacZ | Level 2 Destination vector - Position B - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE | Level 2 - B site (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pTU2-C-lacZ | Level 2 Destination vector - Position C - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE | Level 2 - C site (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pTU2-D-lacZ | Level 2 Destination vector - Position D - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE | Level 2 - D site (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pTU2-A-RFP | Level 2 Destination vector - Position A - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE | Level 2 - A site (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pTU2-C-RFP | Level 2 Destination vector - Position C - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE | Level 2 - C site (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pTU2-D-RFP | Level 2 Destination vector - Position D - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE | Level 2 - D site (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pTU2-a-RFP | Level 2 Destination vector - Position A - Assembly of 2 TU's from Level 1-AB | Level 2 - a site (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pTU2-b-RFP | Level 2 Destination vector - Position B - Assembly of 3 TU's from Level 1-ABC | Level 2 - b site (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP-ORF-eGFP | Level 0 Entry vector" ORF - eGFP " | Enhanced green flourescent protein (eGFP) (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP-ORF-eCFP | Level 0 Entry vector" ORF - eCFP" | Enhanced cyan fluorescent protein (eCFP) (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP-ORF-mCherry | Level 0 Entry vector" ORF - mCherry " | mCherry fluorescent protein (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP-J23100 | Level 0 - J23100 standard iGEM promoter (5' CTAT/3' GTAC fusion) | J23100 promoter (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP-J23108 | Level 0 - J23108 standard iGEM promoter (5' CTAT/3' GTAC fusion) | J23108 promoter (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP-J23114 | Level 0 - J23114 standard iGEM promoter (5' CTAT/3' GTAC fusion) | J23114 promoter (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP-SJM901 | Level 0 - SJM901 promoter (5' CTAT/3' GTAC fusion) | SJM901 promoter (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP-SJM902 | Level 0 - SJM902 promoter (5' CTAT/3' GTAC fusion) | SJM902 promoter (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP-SJM903 | Level 0 - SJM903 promoter (5' CTAT/3' GTAC fusion) | SJM903 promoter (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP-SJM905 | Level 0 - SJM905 promoter (5' CTAT/3' GTAC fusion) | SJM905 promoter (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP-SJM906 | Level 0 - SJM906 promoter (5' CTAT/3' GTAC fusion) | SJM906 promoter (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP-SJM908 | Level 0 - SJM908 promoter (5' CTAT/3' GTAC fusion) | SJM908 promoter (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP-SJM910 | Level 0 - SJM910 promoter (5' CTAT/3' GTAC fusion) | SJM910 promoter (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP-SJM911 | Level 0 - SJM911 promoter (5' CTAT/3' GTAC fusion) | SJM911 promoter (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP-SJM912 | Level 0 - SJM912 promoter (5' CTAT/3' GTAC fusion) | SJM912 promoter (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP-SJM914 | Level 0 - SJM914 promoter (5' CTAT/3' GTAC fusion) | SJM914 promoter (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP-SJM915 | Level 0 - SJM915 promoter (5' CTAT/3' GTAC fusion) | SJM915 promoter (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP-T7 | T7 promoter (5' CTAT/3' GTAC fusion) | T7 promoter (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP-T7_RBS | T7 promoter and RBS - derived from pET3a (5' CTAT/3' CATA) fusion) | T7+RBS (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP-T7_RBS-His6-Thrombin | T7 promoter, RBS, His6-tag and thrombin cleave site - derived from pET15b (5' CTAT/3' CATA) fusion) | T7+RBS+His6+thrombin (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP_BBa_B0034 | Bba_B0034 iGEM RBS (5' GTAC/3' CATA fusion) | Bba_B0034 RBS (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP-pET-RBS | PET RBS - derived from pET vectors (5' GTAC/3' CATA fusion) | pET RBS derived from pET vectors (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP-Tag_linker | N-terminal linker with pET-RBS (5' GTAC/3' TAAA fusion) | N-terminal linker with pET-RBS (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP-HexHis | Hexahistidine N-terminal tag (5' TAAA/3' CATA fusion) | Polyhistidine (HHHHHH) N-terminal tag (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP-Strep(II) | Streptavidin(II) N-terminal tag (5' TAAA/3' CATA fusion) | Strep(II) N-terminal tag (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP-TL1 | RBS_H1 | H1 RBS (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP-TL2
| RBS_H2 | H2 RBS (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP-TL3 | RBS_H3 | H3 RBS (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP-TL4 | RBS_H5 | H5 RBS (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP-TL5 | RBS_M2 | M2 RBS (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP-TL6 | RBS_M3 | M3 RBS (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP-TL7 | RBS_M5 | M5 RBS (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP-TL8 | RBS_L1 | L1 RBS (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP-TL9 | RBS_L2 | L2 RBS (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP-TL10 | RBS_L3 | L3 RBS (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP-TL11 | RBS_L4 | L4 RBS (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP-TL12 | RBS_L5 | L5 RBS (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP-BBa_B0012 | BBa_B0012 iGEM terminator (5' TCGA/ 3' TGTT fusion) | Bba_B0012 terminator (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP-BBa_B0015 | BBa_B0015 iGEM terminator (5' TCGA/ 3' TGTT fusion) | BBa_B0015 terminator (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP-L3S2P21 | L3S2P21 terminator | L3S2P21 terminator (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP-L3S1P51 | L3S1P51 terminator | L3S1P51 terminator (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP-L3S1P32 | L3S1P32 terminator | L3S1P32 terminator (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP-L3S1P11 | L3S1P11 terminator | L3S1P11 terminator (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP-L2U8H11 | L2U8H11 terminator | L2U8H11 terminator (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP-L2U5H11 | L2U5H11 terminator | L2U5H11 terminator (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP-L2U5H08 | L2U5H08 terminator | L2U5H08 terminator (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP-L2U3H03 | L2U3H03 terminator | L2U3H03 terminator (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP-L2U2H09 | L2U2H09 terminator | L2U2H09 terminator (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP-L1U1H09 | L1U1H09 terminator | L1U1H09 terminator (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP-L1U1H08 | L1U1H08 terminator | L1U1H08 terminator (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pTU2_2a | Secondary module (BpiI) site in pTU2a backbone | Secondary module + Golden Gate pTU2a BsmBI destination site (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pTU2_2b | Secondary module (BpiI) site in pTU2b backbone | Secondary module + Golden Gate pTU2b BsmBI destination site (Synthetic) | Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pCfB2899(X-2 MarkerFree) | EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-2 (Chr X: 194944..195980) | | Yeast Expression, CRISPR, Synthetic Biology |
Borodina
| EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9.
Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23.
|
| pCfB3034(X-3 MarkerFree) | EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-3 (Chr X: 223616..224744) | | Yeast Expression, CRISPR, Synthetic Biology |
Borodina
| EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9.
Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23.
|
| pCfB3035(X-4 MarkerFree) | EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-4 (Chr X: 236336..237310) | | Yeast Expression, CRISPR, Synthetic Biology |
Borodina
| EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9.
Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23.
|
| pCfB3036(XI-1 MarkerFree) | EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-1 (Chr XI: 67491..68573) | | Yeast Expression, CRISPR, Synthetic Biology |
Borodina
| EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9.
Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23.
|
| pCfB2903(XI-2 MarkerFree) | EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-2 (Chr XI: 91575..92913) | | Yeast Expression, CRISPR, Synthetic Biology |
Borodina
| EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9.
Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23.
|
| pCfB2904(XI-3 MarkerFree) | EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-3 (Chr XI: 93378..94567) | | Yeast Expression, CRISPR, Synthetic Biology |
Borodina
| EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9.
Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23.
|
| pCfB3037(XI-5 MarkerFree) | EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-5 (Chr XI: 11779..118967) | | Yeast Expression, CRISPR, Synthetic Biology |
Borodina
| EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9.
Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23.
|
| pCfB3038(XII-1 MarkerFree) | EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-1 (Chr XII: 795787..796720) | | Yeast Expression, CRISPR, Synthetic Biology |
Borodina
| EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9.
Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23.
|
| pCfB3039(XII-2 MarkerFree) | EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-2 (Chr XII: 808805..809939) | | Yeast Expression, CRISPR, Synthetic Biology |
Borodina
| EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9.
Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23.
|
| pCfB3040(XII-4 MarkerFree) | EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-4 (Chr XII:830227..831248) | | Yeast Expression, CRISPR, Synthetic Biology |
Borodina
| EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9.
Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23.
|
| pCfB2909(XII-5 MarkerFree) | EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-5 (Chr XII: 839226..840357) | | Yeast Expression, CRISPR, Synthetic Biology |
Borodina
| EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9.
Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23.
|
| pTU2S-a (p15A origin) | Secondary module (BpiI) site in pTU2-a backbone with p15A (low-copy) origin of replication | Golden Gate destination vector - Level 2, 2 TU's with secondary module and p15A origin (Synthetic) | Bacterial Expression, Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pTU2S-b (p15A origin) | Secondary module (BpiI) site in pTU2-a backbone with p15A (low-copy) origin of replication | Golden Gate destination vector - Level 2, 3 TU's with secondary module and p15A origin (Synthetic) | Bacterial Expression, Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pTU2-A-RFP (p15A origin) | pTU2-A backbone, with RFP negative selection and p15A (low-copy) origin of replication | Golden Gate destination vector - Level 2, 4 TU's with RFP and p15A origin (Synthetic) | Bacterial Expression, Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pTU2S-a (colE1 origin) | Secondary module (BpiI) site in pTU2-a backbone with colE1 (medium-copy) origin of replication | Golden Gate destination vector - Level 2, 2 TU's with secondary module and colE1 origin (Synthetic) | Bacterial Expression, Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pTU2S-b (colE1 origin) | Secondary module (BpiI) site in pTU2-a backbone with colE1 (medium-copy) origin of replication | Golden Gate destination vector - Level 2, 3 TU's with secondary module and colE1 origin (Synthetic) | Bacterial Expression, Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pTU2-A-RFP (colE1 origin) | pTU2-A backbone, with RFP negative selection and colE1 (medium-copy) origin of replication | Golden Gate destination vector - Level 2, 4 TU's with RFP and colE1 origin of replication (Synthetic) | Bacterial Expression, Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP-Bba_K822000 | L-Lactate inducible promoter from E. coli MG1655 | Bba_K822000 (L-Lactate promoter - E. coli MG1655) (Other) | Bacterial Expression, Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP-BBa_J23100 | BBa_J23100 promoter (iGEM collection) | Bba_J23100 constitutive promoter (Sigma70) (Synthetic) | Bacterial Expression, Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| pBP-TL9 | TL9 RBS (AAAAAG) | TL9 RBS (Synthetic) | Bacterial Expression, Synthetic Biology |
Freemont
| EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
ACS Synth Biol. 2016 May 2.
|
| p5E--3mnx1 | Can be used to drive expression specifically in zebrafish motor neurons | motor neuron and pancreas homeobox 1 promoter (Danio rerio) | 5' entry vector for Multisite Gateway Three Fragment vector construction kit(Invitrogen) |
Cole
| A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous System and Neurodegenerative Disease.
Zebrafish. 2016 Sep 15.
|
| p5E-gfap | Can be used to drive expression in zebrafish astrocytes. | glial fibrillary acidic protein promoter (Danio rerio) | 5' entry vector for Multisite Gateway Three Fragment vector construction kit (Invitrogen) |
Cole
| A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous System and Neurodegenerative Disease.
Zebrafish. 2016 Sep 15.
|
| pdCas9 (GB1079) | Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part for C-terminal fusions | Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised) (Other) | Plant Expression, CRISPR, Synthetic Biology |
Orzaez
| A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard.
Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. eCollection 2016.
|
| pEGB2Ω2_SF-35s:hCas9:tNos (GB1103) | Transcriptional unit for (human codon optimized) Cas9 plant expression driven by the 35S promoter. Specially conceived to be linked with the TU of the kanamycin resistance gene GB1181. | hCas9 (Other) | Plant Expression, CRISPR, Synthetic Biology |
Orzaez
| A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard.
Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. eCollection 2016.
|
| pEGB 35s:dCas:BRD:tNos (GB1172) | Transcriptional unit of (human codon optimized) inactivated Cas9 fused to the BRD Transcriptional Repressor | dCas9:BRD (Synthetic) | Plant Expression, CRISPR, Synthetic Biology |
Orzaez
| A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard.
Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. eCollection 2016.
|
| pEGB 35s:dCas:VP64:tNos (GB1189) | Transcriptional unit of (human codon optimized) inactivated Cas9 fused to the VP64 Transcriptional Activator | dCas:VP64 (Synthetic) | Plant Expression, CRISPR, Synthetic Biology |
Orzaez
| A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard.
Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. eCollection 2016.
|
| pEGB 35s:dCas:EDLL:tNos (GB1190) | Transcriptional unit of (human codon optimized) inactivated Cas9 fused to the EDLL Transcriptional Activator | dCas:EDLL (Synthetic) | Plant Expression, CRISPR, Synthetic Biology |
Orzaez
| A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard.
Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. eCollection 2016.
|
| pU6-1 (GB1204) | Provides the A. thaliana U6-1 RNA polIII promoter as a level 0 GoldenBraid part | AtU6-1 promoter (Arabidopsis thaliana) | Plant Expression, CRISPR, Synthetic Biology |
Orzaez
| A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard.
Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. eCollection 2016.
|
| tRNA-gRNA position [D1_2] (GB1205) | tRNA and scaffold for the assembly of GBoligomers for the first position (positon [D1_2]) of a polycistronic tRNA-gRNA regulated by the dicot U6-26 or U6-1 promoter (3-part multiplexing) | tRNA-gRNA (Synthetic) | Plant Expression, CRISPR, Synthetic Biology |
Orzaez
| A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard.
Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. eCollection 2016.
|
| tRNA-gRNA position [2_n-1] (GB1206) | tRNA and scaffold for the assembly of GBoligomers for the intermediate position (positon [2_n-1]) of a polycistronic tRNA-gRNA (3-part multiplexing) | tRNA-gRNA position [2_n-1] (Synthetic) | Plant Expression, CRISPR, Synthetic Biology |
Orzaez
| A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard.
Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. eCollection 2016.
|
| tRNA-gRNA position [n] (GB1207) | tRNA and scaffold for the assembly of GBoligomers for the last position (positon [n]) of a polycistronic tRNA-gRNA (2- and 3-part multiplexing) | tRNA-gRNA position [n] (Synthetic) | Plant Expression, CRISPR, Synthetic Biology |
Orzaez
| A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard.
Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. eCollection 2016.
|
| tRNA-gRNA position [M1_2] (GB1209) | tRNA and scaffold for the assembly of GBoligomers for the first position (positon [M1_2]) of a polycistronic tRNA-gRNA regulated by the (monocot) U3 promoter (3-part multiplexing) | tRNA-gRNA position [M1_2] (Synthetic) | Plant Expression, CRISPR, Synthetic Biology |
Orzaez
| A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard.
Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. eCollection 2016.
|
| tRNA-gRNA position [M1_n-1] (GB1210) | tRNA and scaffold for the assembly of GBoligomers for the first position (positon [M1_n-1]) of a polycistronic tRNA-gRNA regulated by the (monocot) U3 promoter (2-part multiplexing) | tRNA-gRNA position [M1_n-1] (Synthetic) | Plant Expression, CRISPR, Synthetic Biology |
Orzaez
| A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard.
Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. eCollection 2016.
|
| pEGB SF-35S:Renilla:TNOS-35S:P19:TNOS (GB0160) | Module for the expression of the Renilla Luciferase with the silencing suppressor P19 | Renilla / P19 (Other) | Plant Expression, Luciferase, Synthetic Biology |
Orzaez
| A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard.
Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. eCollection 2016.
|
| pECM1 | Plasmid that serves as the PCR template to create the genomic pdt1 insertions contained in the EPD library. Plasmid contains the R6K origin of replication so needs pir gene to replicate | pdt1 (Other) | Bacterial Expression |
Collins
| Tunable protein degradation in bacteria.
Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub 2014 Nov 17.
|
| pECT1 | Plasmid that serves as the PCR template to create genomic pdt1 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate | pdt1 (Other) | Bacterial Expression |
Collins
| Tunable protein degradation in bacteria.
Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub 2014 Nov 17.
|
| pECT1B | Plasmid that serves as the PCR template to create genomic pdt1B insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate | pdt1B (Other) | Bacterial Expression |
Collins
| Tunable protein degradation in bacteria.
Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub 2014 Nov 17.
|
| pECT1D | Plasmid that serves as the PCR template to create genomic pdt1D insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate | pdt1D (Other) | Bacterial Expression |
Collins
| Tunable protein degradation in bacteria.
Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub 2014 Nov 17.
|
| pECT1E | Plasmid that serves as the PCR template to create genomic pdt1E insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate | pdt1E (Other) | Bacterial Expression |
Collins
| Tunable protein degradation in bacteria.
Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub 2014 Nov 17.
|
| pECT3 | Plasmid that serves as the PCR template to create genomic pdt3 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate | pdt3 (Other) | Bacterial Expression |
Collins
| Tunable protein degradation in bacteria.
Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub 2014 Nov 17.
|
| pEGB3omega1R Tnos:NptII:Pnos-SF (GB1181) | Transcriptional unit for kanamycin resistance gene | NptII (Other) | Plant Expression, Synthetic Biology |
Orzaez
| A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard.
Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. eCollection 2016.
|
| PhiC31-Neo-ins-5xTetO-pEF-H2B-Citrine-ins | Nuclear fluorescent reporter in mammalian cells, phiC31 attB for integration, can be silenced with TetR-CR or rTetR-CR (e.g. CR can be KRAB or DNMT3B). | Histone H2B - Citrine (Homo sapiens) | Mammalian Expression, Synthetic Biology |
Elowitz
| Dynamics of epigenetic regulation at the single-cell level.
Science. 2016 Feb 12;351(6274):720-4. doi: 10.1126/science.aab2956.
|
| MXS_BidirectionalCAG | Bidirectional CAG promoter (compatible with the MXS Chaining Kit) | Bidirectional CAG Promoter (Synthetic) | Mammalian Expression, Synthetic Biology |
Neveu
| Bidirectional Promoter Engineering for Single Cell MicroRNA Sensors in Embryonic Stem Cells.
PLoS One. 2016 May 6;11(5):e0155177. doi: 10.1371/journal.pone.0155177. eCollection 2016.
|
| 36INS-J23100_AB | Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected.Modified from BBa_J23100. [A:36INS-J23100:B] | Insulated J23100 Promoter (Synthetic) | Synthetic Biology |
Densmore
|
Insulated E. coli promoters
(unpublished)
|
| 36INS-J23100_EB | 36INS-J23100_EB Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_J23100. [E:36INS-J23100:B] | Insulated J23100 Promoter (Synthetic) | Synthetic Biology |
Densmore
|
Insulated E. coli promoters
(unpublished)
|
| 36INS-J23100_FB | Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_J23100. [F:36INS-J23100:B] | Insulated J23100 Promoter (Synthetic) | Synthetic Biology |
Densmore
|
Insulated E. coli promoters
(unpublished)
|
| 36INS-J23100_GB | 36INS-J23100_GB Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_J23100. [G:36INS-J23100:B] | Insulated J23100 Promoter (Synthetic) | Synthetic Biology |
Densmore
|
Insulated E. coli promoters
(unpublished)
|
| 36INS1-pBAD_AB | Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected.Modified from BBa_I13453 to adjust spacing in MoClo system. [A:36INS1-pBAD:B] | Insulated pBAD Promoter (Synthetic) | Synthetic Biology |
Densmore
|
Insulated E. coli promoters
(unpublished)
|
| 36INS1-pBAD_EB | Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. [E:36INS1-pBAD:B] | Insulated pBAD Promoter (Synthetic) | Synthetic Biology |
Densmore
|
Insulated E. coli promoters
(unpublished)
|
| 36INS1-pBAD_FB | Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. [F:36INS1-pBAD:B] | Insulated pBAD Promoter (Synthetic) | Synthetic Biology |
Densmore
|
Insulated E. coli promoters
(unpublished)
|
| 36INS1-pBAD_GB | Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. [G:36INS1-pBAD:B] | Insulated pBAD Promoter (Synthetic) | Synthetic Biology |
Densmore
|
Insulated E. coli promoters
(unpublished)
|
| 36INS2-pBAD_AB | Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [A:36INS2-pBAD:B] | Insulated pBAD Promoter (Synthetic) | Synthetic Biology |
Densmore
|
Insulated E. coli promoters
(unpublished)
|
| 36INS2-pBAD_EB | Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [E:36INS2-pBAD:B] | Insulated pBAD Promoter (Synthetic) | Synthetic Biology |
Densmore
|
Insulated E. coli promoters
(unpublished)
|
| 36INS2-pBAD_FB | Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [F:36INS2-pBAD:B] | Insulated pBAD Promoter (Synthetic) | Synthetic Biology |
Densmore
|
Insulated E. coli promoters
(unpublished)
|
| 36INS2-pBAD_GB | Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [G:36INS2-pBAD:B] | Insulated pBAD Promoter (Synthetic) | Synthetic Biology |
Densmore
|
Insulated E. coli promoters
(unpublished)
|
| 36INS-pTet_AB | Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_R0040. [A:36INS-pTet:B] | Insulated pTet Promoter (Synthetic) | Synthetic Biology |
Densmore
|
Insulated E. coli promoters
(unpublished)
|
| 36INS-pTet_EB | Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_R0040. [E:36INS-pTet:B] | Insulated pTet Promoter (Synthetic) | Synthetic Biology |
Densmore
|
Insulated E. coli promoters
(unpublished)
|
| 36INS-pTet_FB | Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_R0040. [F:36INS-pTet:B] | Insulated pTet Promoter (Synthetic) | Synthetic Biology |
Densmore
|
Insulated E. coli promoters
(unpublished)
|
| 36INS-pTet_GB | Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_R0040. [G:36INS-pTet:B] | Insulated pTet Promoter (Synthetic) | Synthetic Biology |
Densmore
|
Insulated E. coli promoters
(unpublished)
|