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logicgate1.png Synthetic Biology: Mammalian


Browse Addgene's collection of synthetic biology plasmids for use in mammalian systems.






Mammalian Plasmids

Search the table by keyword or sort by the table headings. Click on the publication link to view all plasmids available from the article. Click on the PI link to view all plasmids available from this depositor's lab.

Plasmid Purpose Gene/Insert Vector Type PI Publication
LTRiMammalian Expression, Synthetic Biology Collins A tunable genetic switch based on RNAi and repressor proteins for regulating gene expression in mammalian cells. Cell. 2007 Jul 27. 130(2):363-72.
TLD_026Mammalian Expression, Synthetic Biology Collins A tunable genetic switch based on RNAi and repressor proteins for regulating gene expression in mammalian cells. Cell. 2007 Jul 27. 130(2):363-72.
TLD_009EGFPMammalian Expression, Synthetic Biology Collins A tunable genetic switch based on RNAi and repressor proteins for regulating gene expression in mammalian cells. Cell. 2007 Jul 27. 130(2):363-72.
TLD_010EGFPMammalian Expression, Synthetic Biology Collins A tunable genetic switch based on RNAi and repressor proteins for regulating gene expression in mammalian cells. Cell. 2007 Jul 27. 130(2):363-72.
TLD_027Mammalian Expression, Synthetic Biology Collins A tunable genetic switch based on RNAi and repressor proteins for regulating gene expression in mammalian cells. Cell. 2007 Jul 27. 130(2):363-72.
TLD_062Mammalian Expression, Synthetic Biology Collins A tunable genetic switch based on RNAi and repressor proteins for regulating gene expression in mammalian cells. Cell. 2007 Jul 27. 130(2):363-72.
pAL149pCMV-PhyB(1-908)-10aaLinker-mCherry-Kras4BCT (Arabidopsis thaliana)Mammalian Expression, Synthetic Biology Voigt Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature. 2009 Sep 13. ():.
pAL175p!SV40-mYFP-PIF6APB (Arabidopsis thaliana)Mammalian Expression, Synthetic Biology Voigt Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature. 2009 Sep 13. ():.
pAL188p!SV40-TiamDHPH-20aaLinker-mYFP-PIF6APB (Arabidopsis thaliana)Mammalian Expression, Synthetic Biology Voigt Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature. 2009 Sep 13. ():.
pAL189p!SV40-IntersectinDHPH-20aaLinker-mYFP-PIF6APB (Arabidopsis thaliana)Mammalian Expression, Synthetic Biology Voigt Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature. 2009 Sep 13. ():.
pAL190p!SV40-TimDHPH-20aaLinker-mYFP-PIF6APB (Arabidopsis thaliana)Mammalian Expression, Synthetic Biology Voigt Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature. 2009 Sep 13. ():.
pAL197p!SV40-PakGBD-mCherry (Arabidopsis thaliana)Mammalian Expression, Synthetic Biology Voigt Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature. 2009 Sep 13. ():.
pAL198p!SV40-mCherry-WaspGBD (Arabidopsis thaliana)Mammalian Expression, Synthetic Biology Voigt Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature. 2009 Sep 13. ():.
pAL204pCMV-PhyB(1-908)-10aaLinker-mCFP-Kras4BCT (Arabidopsis thaliana)Mammalian Expression, Synthetic Biology Voigt Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature. 2009 Sep 13. ():.
pAL113pCMV-PhyBNT(Y276H)-10aaLinker-mCFP-HrasCT (Mus musculus)Mammalian Expression, Synthetic Biology Voigt Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature. 2009 Sep 13. ():.
pAL101pCMV-PIF6APB-mYFP (Mus musculus)Mammalian Expression, Synthetic Biology Voigt Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature. 2009 Sep 13. ():.
pAL130pCMV-PIF3NT-mYFP (Mus musculus)Mammalian Expression, Synthetic Biology Voigt Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature. 2009 Sep 13. ():.
pDN-D2irTNG4kwhpCMV-D2i promoter (Synthetic), htetR::NLS::EGFP (Homo sapiens)Mammalian Expression, Synthetic Biology ; Expression regulator/reporter; Expression "Linearizer" system Balazsi Transferring a synthetic gene circuit from yeast to mammalian cells. Nat Commun. 2013 Feb 5;4:1451. doi: 10.1038/ncomms2471.
pDN-D2irG4kwhpCMV-D2i promoter (Synthetic), EGFP (Homo sapiens)Mammalian Expression, Synthetic Biology ; Expression regulator/reporter Balazsi Transferring a synthetic gene circuit from yeast to mammalian cells. Nat Commun. 2013 Feb 5;4:1451. doi: 10.1038/ncomms2471.
pDN-D2irTN6kwhpCMV-D2i promoter (Synthetic), htetR::NLS (Homo sapiens)Mammalian Expression, Synthetic Biology ; Expression regulator/reporter; Expression "Linearizer" system Balazsi Transferring a synthetic gene circuit from yeast to mammalian cells. Nat Commun. 2013 Feb 5;4:1451. doi: 10.1038/ncomms2471.
pDN-D2irC6kwhpCMV-D2i promoter (Synthetic), mCherry (Homo sapiens)Mammalian Expression, Synthetic Biology ; Expression regulator/reporter Balazsi Transferring a synthetic gene circuit from yeast to mammalian cells. Nat Commun. 2013 Feb 5;4:1451. doi: 10.1038/ncomms2471.
pCOCK CMVwtMammalian expression plasmid with EGFP expressed from CMV wild-type promoter.CMV wt promoter (Other)Mammalian Expression, Synthetic Biology Wang Modulating ectopic gene expression levels by using retroviral vectors equipped with synthetic promoters. Syst Synth Biol. 2011 Dec;5(3-4):131-8. doi: 10.1007/s11693-011-9089-0. Epub 2011 Nov 20.
pCOCK CM-C18Mammalian expression plasmid with EGFP expressed from synthetic promoter (CMVwt CAAT box mutant).CMV mutant promoter (Other)Mammalian Expression, Synthetic Biology Wang Modulating ectopic gene expression levels by using retroviral vectors equipped with synthetic promoters. Syst Synth Biol. 2011 Dec;5(3-4):131-8. doi: 10.1007/s11693-011-9089-0. Epub 2011 Nov 20.
pCOCK CM-C02Mammalian expression plasmid with EGFP expressed from synthetic promoter (CMVwt CAAT box mutant).CMV mutant promoter (Other)Mammalian Expression, Synthetic Biology Wang Modulating ectopic gene expression levels by using retroviral vectors equipped with synthetic promoters. Syst Synth Biol. 2011 Dec;5(3-4):131-8. doi: 10.1007/s11693-011-9089-0. Epub 2011 Nov 20.
pCOCK CM-R03Mammalian expression plasmid with EGFP expressed from synthetic promoter (random mutagenesis of CMVwt 3' end).CMV mutant promoter (Other)Mammalian Expression, Synthetic Biology Wang Modulating ectopic gene expression levels by using retroviral vectors equipped with synthetic promoters. Syst Synth Biol. 2011 Dec;5(3-4):131-8. doi: 10.1007/s11693-011-9089-0. Epub 2011 Nov 20.
pCOCK CM-R05Mammalian expression plasmid with EGFP expressed from synthetic promoter (random mutagenesis of CMVwt 3' end).CMV mutant promoter (Other)Mammalian Expression, Synthetic Biology Wang Modulating ectopic gene expression levels by using retroviral vectors equipped with synthetic promoters. Syst Synth Biol. 2011 Dec;5(3-4):131-8. doi: 10.1007/s11693-011-9089-0. Epub 2011 Nov 20.
pCru5-ACC/ACC/ACC/ACC-mEGFP-IRES-mCherryRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.mEGFP (Synthetic), mCherry (Synthetic)Mammalian Expression, Retroviral, Synthetic Biology Wang Tuning gene expression with synthetic upstream open reading frames. Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24.
pCru5-ACC/ACC/ACC-mEGFP-IRES-mCherryRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.mEGFP (Synthetic), mCherry (Synthetic)Mammalian Expression, Retroviral, Synthetic Biology Wang Tuning gene expression with synthetic upstream open reading frames. Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24.
pCru5-ACC/ACC-mEGFP-IRES-mCherryRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.mEGFP (Synthetic), mCherry (Synthetic)Mammalian Expression, Retroviral, Synthetic Biology Wang Tuning gene expression with synthetic upstream open reading frames. Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24.
pCru5-GGG/ACC-mEGFP-IRES-mCherryRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.mEGFP (Synthetic), mCherry (Synthetic)Mammalian Expression, Retroviral, Synthetic Biology Wang Tuning gene expression with synthetic upstream open reading frames. Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24.
pCru5-CCC/ACC-mEGFP-IRES-mCherryRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.mEGFP (Synthetic), mCherry (Synthetic)Mammalian Expression, Retroviral, Synthetic Biology Wang Tuning gene expression with synthetic upstream open reading frames. Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24.
pCru5-UCU/ACC-mEGFP-IRES-mCherryRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.mEGFP (Synthetic), mCherry (Synthetic)Mammalian Expression, Retroviral, Synthetic Biology Wang Tuning gene expression with synthetic upstream open reading frames. Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24.
pCru5-/CGA-mEGFP-IRES-mCherryRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.mEGFP (Synthetic), mCherry (Synthetic)Mammalian Expression, Retroviral, Synthetic Biology Wang Tuning gene expression with synthetic upstream open reading frames. Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24.
pCru5-/UAA-mEGFP-IRES-mCherryRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.mEGFP (Synthetic), mCherry (Synthetic)Mammalian Expression, Retroviral, Synthetic Biology Wang Tuning gene expression with synthetic upstream open reading frames. Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24.
pCru5-/GCCACC-mEGFP-IRES-mCherryRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.mEGFP (Synthetic), mCherry (Synthetic)Mammalian Expression, Retroviral, Synthetic Biology Wang Tuning gene expression with synthetic upstream open reading frames. Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24.
pWPT-ACC/ACC/ACC/ACC-mEGFP-IRES-mCherryLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.mEGFP (Synthetic), mCherry (Synthetic)Mammalian Expression, Lentiviral, Synthetic Biology Wang Tuning gene expression with synthetic upstream open reading frames. Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24.
pWPT-ACC/ACC/ACC-mEGFP-IRES-mCherryLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.mEGFP (Synthetic), mCherry (Synthetic)Mammalian Expression, Lentiviral, Synthetic Biology Wang Tuning gene expression with synthetic upstream open reading frames. Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24.
pWPT-ACC/ACC-mEGFP-IRES-mCherryLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.mEGFP (Synthetic), mCherry (Synthetic)Mammalian Expression, Lentiviral, Synthetic Biology Wang Tuning gene expression with synthetic upstream open reading frames. Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24.
pWPT-GGG/ACC-mEGFP-IRES-mCherryLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.mEGFP (Synthetic), mCherry (Synthetic)Mammalian Expression, Lentiviral, Synthetic Biology Wang Tuning gene expression with synthetic upstream open reading frames. Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24.
pWPT-CCC/ACC-mEGFP-IRES-mCherryLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.mEGFP (Synthetic), mCherry (Synthetic)Mammalian Expression, Lentiviral, Synthetic Biology Wang Tuning gene expression with synthetic upstream open reading frames. Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24.
pWPT-UCU/ACC-mEGFP-IRES-mCherryLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.mEGFP (Synthetic), mCherry (Synthetic)Mammalian Expression, Lentiviral, Synthetic Biology Wang Tuning gene expression with synthetic upstream open reading frames. Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24.
pWPT-/CGA-mEGFP-IRES-mCherryLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.mEGFP (Synthetic), mCherry (Synthetic)Mammalian Expression, Lentiviral, Synthetic Biology Wang Tuning gene expression with synthetic upstream open reading frames. Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24.
pWPT-/UAA-mEGFP-IRES-mCherryLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.mEGFP (Synthetic), mCherry (Synthetic)Mammalian Expression, Lentiviral, Synthetic Biology Wang Tuning gene expression with synthetic upstream open reading frames. Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24.
pWPT-/GCCACC-mEGFP-IRES-mCherryLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.mEGFP (Synthetic), mCherry (Synthetic)Mammalian Expression, Lentiviral, Synthetic Biology Wang Tuning gene expression with synthetic upstream open reading frames. Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24.
pHR SFFVp PHAkt CeruleanFluorescent reporter for membrane PIP3 levelsPHAktLentiviral, Synthetic Biology Lim Light-based feedback for controlling intracellular signaling dynamics. Nat Methods. 2011 Sep 11;8(10):837-9. doi: 10.1038/nmeth.1700.
pHR SFFVp Phy-mCherry-CAAXOptogenetic PI3K activation system; membrane componentPhyB 1-908 (Arabidopsis thaliana)Lentiviral, Synthetic Biology Lim Light-based feedback for controlling intracellular signaling dynamics. Nat Methods. 2011 Sep 11;8(10):837-9. doi: 10.1038/nmeth.1700.
pHR iSH-YFP-PIFOptogenetic PI3K activation system; cytoplasmic componentp85 iSH2 (Mus musculus), PIF6 1-100 (Arabidopsis thaliana)Lentiviral, Synthetic Biology Lim Light-based feedback for controlling intracellular signaling dynamics. Nat Methods. 2011 Sep 11;8(10):837-9. doi: 10.1038/nmeth.1700.
pHR SFFVp BFP-Erk2Fluorescent reporter for Erk nuclear translocationErk2 (Mus musculus)Lentiviral Lim Using optogenetics to interrogate the dynamic control of signal transmission by the ras/erk module. Cell. 2013 Dec 5;155(6):1422-34. doi: 10.1016/j.cell.2013.11.004.
pHR SFFVp BFP-PIF-SOS2catOptogenetic Ras activation system; cytoplasmic componentSOS2cat (Homo sapiens), PIF6 1-100 (Arabidopsis thaliana)Lentiviral Lim Using optogenetics to interrogate the dynamic control of signal transmission by the ras/erk module. Cell. 2013 Dec 5;155(6):1422-34. doi: 10.1016/j.cell.2013.11.004.
pHR SFFVp YFP-PIF-SOS2catOptogenetic Ras activation system; cytoplasmic componentSOS2cat (Homo sapiens), PIF6 1-100 (Arabidopsis thaliana)Lentiviral Lim Using optogenetics to interrogate the dynamic control of signal transmission by the ras/erk module. Cell. 2013 Dec 5;155(6):1422-34. doi: 10.1016/j.cell.2013.11.004.
pExp_CAG:EBFP2Constitutively expresses blue fluorescent protein, EBFP2EBFP2 (Synthetic)Mammalian Expression, Synthetic Biology Weiss Accurate Predictions of Genetic Circuit Behavior from Part Characterization and Modular Composition. ACS Synth Biol. 2014 Nov 17.
pExp_CAG:EYFPConstitutively expresses yellow fluorescent protein , EYFPEYFPMammalian Expression, Synthetic Biology Weiss Accurate Predictions of Genetic Circuit Behavior from Part Characterization and Modular Composition. ACS Synth Biol. 2014 Nov 17.
pExp_TRE:EBFP2Inducible expression of EBFP2 with rtTA for input fluorescenceEBFP2Mammalian Expression, Synthetic Biology Weiss Accurate Predictions of Genetic Circuit Behavior from Part Characterization and Modular Composition. ACS Synth Biol. 2014 Nov 17.
pExp_TRE:TAL14Inducible expression of TAL14 as input repressorTAL14Mammalian Expression, Synthetic Biology Weiss Accurate Predictions of Genetic Circuit Behavior from Part Characterization and Modular Composition. ACS Synth Biol. 2014 Nov 17.
pExp_TRE:TAL21Inducible expression of TAL21 as input repressorTAL21Mammalian Expression, Synthetic Biology Weiss Accurate Predictions of Genetic Circuit Behavior from Part Characterization and Modular Composition. ACS Synth Biol. 2014 Nov 17.
CMVp-dCas9-3xNLS-VP64 (Construct 1)Expresses taCas9 in Mammalian cells for transactivating endogenous and synthetic promoters. The backbone is a lentiviral vector.dCas9 (Homo sapiens)Mammalian Expression, CRISPR, Synthetic Biology Lu Multiplexed and Programmable Regulation of Gene Networks with an Integrated RNA and CRISPR/Cas Toolkit in Human Cells. Mol Cell. 2014 May 14. pii: S1097-2765(14)00355-4. doi: 10.1016/j.molcel.2014.04.022.
PGK1p-Csy4-pA (Construct 2)Expresses Csy4 in Mammalian cells for site-specific RNA processingCsy4/Cas6 (Homo sapiens)Mammalian Expression, Synthetic Biology Lu Multiplexed and Programmable Regulation of Gene Networks with an Integrated RNA and CRISPR/Cas Toolkit in Human Cells. Mol Cell. 2014 May 14. pii: S1097-2765(14)00355-4. doi: 10.1016/j.molcel.2014.04.022.
P1-EYFP-pA (Construct 5)Synthetic promoter responsive to gRNA1EYFP (Homo sapiens)Mammalian Expression, Synthetic Biology Lu Multiplexed and Programmable Regulation of Gene Networks with an Integrated RNA and CRISPR/Cas Toolkit in Human Cells. Mol Cell. 2014 May 14. pii: S1097-2765(14)00355-4. doi: 10.1016/j.molcel.2014.04.022.
P2-ECFP-pA (Construct 6)Synthetic promoter responsive to gRNA2ECFP (Homo sapiens)Mammalian Expression, Synthetic Biology Lu Multiplexed and Programmable Regulation of Gene Networks with an Integrated RNA and CRISPR/Cas Toolkit in Human Cells. Mol Cell. 2014 May 14. pii: S1097-2765(14)00355-4. doi: 10.1016/j.molcel.2014.04.022.
CMVp-ECFP-Triplex-28-8xmiRNA-BS-28-pA (Construct 22)CFP reporter along with FF4 miRNA binding sites and 28 nt sequencesECFP (Homo sapiens)Mammalian Expression, Synthetic Biology Lu Multiplexed and Programmable Regulation of Gene Networks with an Integrated RNA and CRISPR/Cas Toolkit in Human Cells. Mol Cell. 2014 May 14. pii: S1097-2765(14)00355-4. doi: 10.1016/j.molcel.2014.04.022.
CMVp-dsRed2-Triplex-28-gRNA1-28-pAPlasmid encoding the Triplex/gRNA architecture.This is a modified form of the original plasmid described in the paper (Construct 3). mKate2 was replaced with dsRed2 because of distribution issues.dsRed2 (Homo sapiens)Mammalian Expression, Synthetic Biology Lu Multiplexed and Programmable Regulation of Gene Networks with an Integrated RNA and CRISPR/Cas Toolkit in Human Cells. Mol Cell. 2014 May 14. pii: S1097-2765(14)00355-4. doi: 10.1016/j.molcel.2014.04.022.
CMVp-dsRed2-Triplex-HHRibo-gRNA1-HDVRibo-pAPlasmid encoding the Ribozyme/gRNA architecture. This is a modified form of the original plasmid described in the paper (Construct 13). mKate2 was replaced with dsRed2 because of distribution issues.dsRed2 (Homo sapiens)Mammalian Expression, Synthetic Biology Lu Multiplexed and Programmable Regulation of Gene Networks with an Integrated RNA and CRISPR/Cas Toolkit in Human Cells. Mol Cell. 2014 May 14. pii: S1097-2765(14)00355-4. doi: 10.1016/j.molcel.2014.04.022.
CMVp-dsRed2-Triplex-28-gRNA3-28-gRNA4-28-gRNA5-28-gRNA6-28-pAPlasmid encoding multiplexed 4x gRNAs. This is a modified form of the original plasmid described in the paper (Construct 19). mKate2 was replaced with dsRed2 because of distribution issues.dsRed2 (Homo sapiens)Mammalian Expression, Synthetic Biology Lu Multiplexed and Programmable Regulation of Gene Networks with an Integrated RNA and CRISPR/Cas Toolkit in Human Cells. Mol Cell. 2014 May 14. pii: S1097-2765(14)00355-4. doi: 10.1016/j.molcel.2014.04.022.
tTA_BFPConstitutive expression of tTA-EBFP fusion protein (tTA from CL-CTIG, Addgene plasmid 14901)tTA transcription factor fused to enhanced blue fluorescent protein (Synthetic)Mammalian Expression Leonard Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11.
pBI-MCS-EYFPThe tet-responsive reporter pBI-MCS-EGFP (Addgene plasmid 16542) was modified to express YFP instead of GFPenhanced yellow fluorescent protein (Synthetic)Mammalian Expression Leonard Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11.
mCherry_LD 0_CS G_tTA-BFPMESA target chain with mCherry ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion MESA target chain with mCherry ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence (Synthetic)Mammalian Expression, AAV Leonard Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11.
mCherry_LD 0_TEVMESA protease chain with mCherry ectodomain and no linker domainMESA protease chain with mCherry ectodomain and TEV protease (Synthetic)Mammalian Expression, AAV Leonard Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11.
CD4_LD 0_CS G_tTA-BFPMESA target chain with CD4 ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion MESA target chain with CD4 ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence (Synthetic)Mammalian Expression, AAV Leonard Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11.
CD4_LD 0_TEVMESA protease chain with CD4 ectodomain and no linker domainMESA protease chain with CD4 ectodomain and TEV protease (Synthetic)Mammalian Expression, AAV Leonard Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11.
dTomato_LD 0_CS G_tTA-BFPMESA target chain with dTomato ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion MESA target chain with dTomato ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence (Synthetic)Mammalian Expression, AAV Leonard Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11.
dTomato_LD 0_TEVMESA protease chain with dTomato ectodomain and no linker domainMESA protease chain with dTomato ectodomain and TEV protease (Synthetic)Mammalian Expression, AAV Leonard Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11.
mCherry_LD 0_CS A_tTA-BFPMESA target chain with mCherry ectodomain, no linker domain, A cleavage sequence, and tTA-BFP fusion MESA target chain with mCherry ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (A in P1') (Synthetic)Mammalian Expression, AAV Leonard Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11.
mCherry_LD 0_CS M_tTA-BFPMESA target chain with mCherry ectodomain, no linker domain, M cleavage sequence, and tTA-BFP fusion MESA target chain with mCherry ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1') (Synthetic)Mammalian Expression, AAV Leonard Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11.
dTomato_LD 0_CS M_tTA-BFPMESA target chain with dTomato ectodomain, no linker domain, M cleavage sequence, and tTA-BFP fusion MESA target chain with dTomato ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1') (Synthetic)Mammalian Expression, AAV Leonard Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11.
FRB_LD 0_CS G_tTA-BFPMESA target chain with Rapamycin FRB ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion MESA target chain with FRB ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence (Synthetic)Mammalian Expression, AAV Leonard Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11.
FKBP_LD 0_TEVMESA protease chain with Rapamycin FKBP ectodomain and no linker domainMESA protease chain with FKBP rapamycin-binding ectodomain, TEV protease and mCherry fusion (Synthetic)Mammalian Expression, AAV Leonard Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11.
FKBP_SCF2_LD6_NTEVMESA split-TEV protease chain with Rapamycin FKBP ectodomain, 2 aa scaffold, 6 aa linker domain, N-terminal TEV proteaseMESA split protease chain with FKBP rapamycin-binding ectodomain and N terminal fragment of TEV protease (Synthetic)Mammalian Expression, AAV Leonard Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11.
FRB_SCF2_LD6_CTEVMESA split-TEV protease chain with Rapamycin FRB ectodomain 2 aa scaffold, 6 aa linker domain, C-terminal TEV proteaseMESA split protease chain with FRB rapamycin-binding ectodomain and C terminal fragment of TEV protease (Synthetic)Mammalian Expression, AAV Leonard Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11.
pTK095SIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak L7Ae-P2A-EYFP 4xFF4 8xMS2SGP(14) Kozak L7Ae-P2A-EYFP 4xFF4 8xMS2 Weiss Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery. Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301.
pTK101SIN SP6 P1234 (nsP2 P726S) SGP(14) mKate-G8-L7Ae-PEST 4xFF4SGP(14) mKate-G8-L7Ae-PEST 4xFF4 Weiss Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery. Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301.
pTK105SIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn EYFP-PESTSGP(14) 2xK-turn EYFP-PEST Weiss Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery. Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301.
pTK194TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak mKate opal attB2 AscI (truncated E1) 3'UTR Poly A I-SceISGP XbaI attB1 Kozak mKate opal attB2 AscI Weiss Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery. Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301.
pTK295SIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn Kozak EGFPSGP(14) 2xK-turn Kozak EGFP Weiss Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery. Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301.
pTK296SIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn Kozak EGFP-PESTSGP(14) 2xK-turn Kozak EGFP-PEST Weiss Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery. Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301.
pTK297SIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak mKate-G8-L7Ae 4xFF4SGP(14) Kozak mKate-G8-L7Ae 4xFF4 Weiss Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery. Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301.
pTK298SIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak mKate-G8-L7Ae-PEST 4xFF4SGP(14) Kozak mKate-G8-L7Ae-PEST 4xFF4 Weiss Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery. Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301.
pTK299SIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak L7Ae-P2A-EYFP 4xFF4SGP(14) Kozak L7Ae-P2A-EYFP 4xFF4 Weiss Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery. Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301.
pTK312TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak EGFP ochre attB2 AscI (truncated E1) 3'UTR Poly A I-SceISGP XbaI attB1 Kozak EGFP ochre attB2 AscI Weiss Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery. Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301.
pTK313TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak EGFP-PEST ochre attB2 AscI (truncated E1) 3'UTR Poly A I-SceISGP XbaI attB1 Kozak EGFP-PEST ochre attB2 AscI Weiss Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery. Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301.
pTK317TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP(16) 2xK-turn Kozak EGFP attB2 AscI (truncated E1) 3'UTR Poly A I-SceISGP(16) 2xK-turn Kozak EGFP attB2 AscI Weiss Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery. Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301.
pTK331TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP(14) Kozak mKate-G8-L7Ae-PEST 4xFF4 attB2 SGP2(98/30) XbaI attB1 EBFP2 ochre attB2 (insert) AscI (truncated E1) 3'UTR Poly A I-SceISGP(16) 2xK-turn Kozak EGFP attB2 AscI Weiss Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery. Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301.
pTK332SIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2-4xFF5SGP(14) 2xK-turn Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2-4xFF5 Weiss Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery. Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301.
pTK333SIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2 4xFF5SGP(14) Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2 4xFF5 Weiss Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery. Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301.
pcDNA-dCas9-HAencodes c-terminal HA-tagged S. pyogenes dCas9 driven by CMV promoterS.pyogenes dCas9 with c-terminal HA tag (Synthetic)Mammalian Expression, CRISPR, Synthetic Biology Gersbach Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers. Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6.
pcDNA-dCas9-FLp300encodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 (aa 2-2414) driven by CMV promoterS.pyogenes dCas9 with c-terminal full length human p300 (aa 2-2414) (Homo sapiens)Mammalian Expression, CRISPR, Synthetic Biology Gersbach Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers. Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6.
pcDNA-dCas9-p300 Coreencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoterS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (Homo sapiens)Mammalian Expression, CRISPR, Synthetic Biology Gersbach Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers. Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6.
pcDNA-dCas9-p300 Core (D1399Y)encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV promoterS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (Homo sapiens)Mammalian Expression, CRISPR, Synthetic Biology Gersbach Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers. Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6.
pcDNA-dCas9-p300 Core (1645/1646 RR/EE)encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation 1645/1646 RR/EE) driven by CMV promoterS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (Homo sapiens)Mammalian Expression, CRISPR, Synthetic Biology Gersbach Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers. Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6.
pcDNA-dCas9-p300 Core (C1204R)encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation C1204R) driven by CMV promoterS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (Homo sapiens)Mammalian Expression, CRISPR, Synthetic Biology Gersbach Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers. Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6.
pcDNA-dCas9-p300 Core (Y1467F)encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inavtivating mutation Y1467F) driven by CMV promoterS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (Homo sapiens)Mammalian Expression, CRISPR, Synthetic Biology Gersbach Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers. Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6.
pcDNA-dCas9-p300 Core (1396/1397 SY/WW)encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation 1396/1397 SY/WW) driven by CMV promoterS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (Homo sapiens)Mammalian Expression, CRISPR, Synthetic Biology Gersbach Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers. Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6.
pcDNA-dCas9-p300 Core (H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A)encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutations H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A) driven by CMV promoterS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (Homo sapiens)Mammalian Expression, CRISPR, Synthetic Biology Gersbach Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers. Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6.
pcDNA3.3-Nm-dCas9-p300 Coreencodes N. meningiditis dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoterNm-dCas9-p300 Core (Homo sapiens)Mammalian Expression, CRISPR, Synthetic Biology Gersbach Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers. Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6.
pZDonor-Nm-Cas9-gRNA-hU6empty backbone for cloning N. meningiditis protospacers. Encodes constant region of N. meningiditis Cas9 synthetic gRNAMammalian Expression, CRISPR, Synthetic Biology Gersbach Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers. Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6.
Ai62(TITL-tdT) Flp-in replacement vectorRecombinase-mediated cassette exchange in ES cells to insert a Cre & tTA-dependent tdTomato expression cassette into a docking site within the mouse TIGRE genomic locuschromatin insulator flanked TRE-LSL-tdTomato (Synthetic)Recombinase-mediated cassette exchange using Flp recombinase Zeng Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance. Neuron. 2015 Mar 4;85(5):942-58. doi: 10.1016/j.neuron.2015.02.022.
pJZC625sgRNA with 1x MS2, Pol II promoter with ribozyme-gRNA-ribozyme design for yeast cellssgRNA +1x MS2, Pol II promoter with ribozyme cleavageYeast Expression Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
pJZC32sgRNA (no RNA aptamer addition) with MCP-VP64 effector for mammalian cellssgRNA, MCP-VP64Mammalian Expression, Lentiviral Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
pJZC25sgRNA + 1x MS2 with MCP-VP64 effector for mammalian cellssgRNA + 1x MS2 binding module, MCP-VP64Mammalian Expression, Lentiviral Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
pJZC33sgRNA + 2x MS2 with MCP-VP64 effector for mammalian cellssgRNA + 2x MS2 binding module, MCP-VP64Mammalian Expression, Lentiviral Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
pJZC34sgRNA + 2x MS2(wt+f6) with MCP-VP64 effector for mammalian cellssgRNA + 2x MS2(wt+f6) binding module, MCP-VP64Mammalian Expression, Lentiviral Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
pJZC41sgRNA (no RNA aptamer addition) with PCP-VP64 effector for mammalian cellssgRNA, PCP-VP64Mammalian Expression, Lentiviral Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
pJZC39sgRNA + 1x PP7 with mCherry for mammalian cellssgRNA + 1x PP7, mCherryMammalian Expression, Lentiviral Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
pJZC40sgRNA + 2x PP7 with mCherry for mammalian cellssgRNA + 2x PP7, mCherryMammalian Expression, Lentiviral Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
pJZC101sgRNA (no RNA aptamer addition) with COM-VP64 effector for mammalian cellssgRNA, COM-VP64Mammalian Expression, Lentiviral Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
pJZC48sgRNA + 1x COM with COM-VP64 effector for mammalian cellssgRNA + 1x COM binding module, COM-VP64Mammalian Expression, Lentiviral Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
pJZC77sgRNA (no RNA aptamer addition) with COM-KRAB effector for mammalian cellssgRNA, COM-KRABMammalian Expression, Lentiviral Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
pJZC78sgRNA + 1x COM with COM-KRAB effector for mammalian cellssgRNA + 1x COM binding module, COM-KRABMammalian Expression, Lentiviral Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
pJZC73sgRNA (no RNA aptamer addition) with COM-KRAB effector for mammalian cellssgRNA, COM-KRABMammalian Expression, Lentiviral Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
pJZC74sgRNA + 1x COM with COM-KRAB effector for mammalian cellssgRNA + 1x COM binding module, COM-KRABMammalian Expression, Lentiviral Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
pJZC116sgRNA + 2x MS2(wt+f6) with MCP-VP64 effector for mammalian cells, marked by BFPsgRNA + 2x MS2 (wt+f6) binding module, MCP-VP64Mammalian Expression, Lentiviral Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
MXS_chaining vectorEmpty BackboneSynthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_MCS1MXS_chaining vector with MCS1EcoRV-MluI-XhoI-NheI-XbaI-NcoI-KpnI-BclI-BamHI-SalI MCS (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_MCS2MXS_chaining vector with MCS2EcoRV-MluI-XhoI-EcoRI-BglII-KpnI-SalI MCS (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_MCS3MXS_chaining vector with MCS3MluI-XhoI-HindIII-PstI-SalI MCS (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_MCS4MXS_chaining vector with MCS4MluI-XhoI-PvuII-BglII-SalI MCS (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
SMX DEST vectorEmpty BackboneSynthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_TagBFPMXS_chaining vector with TagBFPTagBFP (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_CeruleanMXS_chaining vector with CeruleanCerulean (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_LSS-mKate2MXS_chaining vector with LSS-mKate2LSS-mKate2 (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_mEGFPMXS_chaining vector with mEGFPmEGFP (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_mAzamiGreenMXS_chaining vector with mAzamiGreenmAzamiGreen (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_CitrineMXS_chaining vector with CitrineCitrine (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_mKO2MXS_chaining vector with mKO2mKO2 (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_tdTomatoMXS_chaining vector with tdTomatotdTomato (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_mCherryMXS_chaining vector with mCherrymCherry (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_mPlumMXS_chaining vector with mPlummPlum (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_E2-CrimsonMXS_chaining vector with E2-CrimsonE2-Crimson (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_iRFP670MXS_chaining vector with iRFP670iRFP670 (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_PA-GFPMXS_chaining vector with PA-GFPPA-GFP (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_KaedeMXS_chaining vector with KaedeKaede (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_PGKMXS_chaining vector with PGKPGK Promoter (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_minPGKMXS_chaining vector with minPGKMinimal PGK Promoter (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_PGKenhancerMXS_chaining vector with PGKenhancerPGK enhancer (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_CMVMXS_chaining vector with CMVCMV Promoter (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_minCMVMXS_chaining vector with minCMVMinimal CMV Promoter (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_CMVenhancerMXS_chaining vector with CMVenhancerCMV enhancer (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_CAGMXS_chaining vector with CAGCAG Promoter (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_EF1aMXS_chaining vector with EF1aEF1a Promoter (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_TetMXS_chaining vector with TetTetracycline inducible promoter (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_BiTetMXS_chaining vector with BiTetBidirectional tetracycline inducible promoter (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_bGpAMXS_chaining vector with bGpAbeta-Globin poly A (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_bGHpAMXS_chaining vector with bGHpABovine growth hormone poly A (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_SV40pAMXS_chaining vector with SV40pASV40 poly A (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_CreERT2MXS_chaining vector with CreERT2Tamoxifen inducible Cre recombinase (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_rtTA3MXS_chaining vector with rtTA3Reverse tetracycline transactivator (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_tTA2MXS_chaining vector with rTA2Tetracycline transactivator (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_loxPMXS_chaining vector with loxPloxP site (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_linkerMXS_chaining vector with linkerLinker 12AA Gly-/Ser-rich (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_H2BMXS_chaining vector with H2BHistone 2B with leading Kozak sequence (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_P2AMXS_chaining vector with P2ASelf-cleaving peptide (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_PEST2DMXS_chaining vector with PEST2D Destabilization tag (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_CMV::HygroR-bGHpAMXS_chaining vector with CMV::HygroR-bGHpA resistance cassette against hygromycin B with CMV Promoter (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_PGK::HygroR-bGHpAMXS_chaining vector with PGK::HygroR-bGHpA resistance cassette against hygromycin B with PGK Promoter (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_CMV::NeoR-bGHpAMXS_chaining vector with CMV::NeoR-bGHpA resistance cassette against Neomycin with CMV Promoter (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_PGK::NeoR-bGHpAMXS_chaining vector with PGK::NeoR-bGHpA resistance cassette against Neomycin with PGK Promoter (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_CMV::PuroR-bGHpAMXS_chaining vector with CMV::PuroR-bGHpA resistance cassette against Puromycin with CMV Promoter (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_PGK::PuroR-bGHpAMXS_chaining vector with PGK::PuroR-bGHpA resistance cassette against Puromycin with PGK Promoter (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_CMV::ZeoR-bGHpAMXS_chaining vector with CMV::ZeoR-bGHpA resistance cassette against Zeocin with CMV Promoter (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_PGK::ZeoR-bGHpAMXS_chaining vector with PGK::ZeoR-bGHpA resistance cassette against Zeocin with PGK Promoter (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_CMV::CreERT2-bGHpAMXS_chaining vector with CMV::CreERT2-bGHpA cassette containing the tamoxifen inducible Cre recombinase with CMV Promoter (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_PGK::CreERT2-bGHpAMXS_chaining vector with PGK::CreERT2-bGHpA cassette containing the tamoxifen inducible Cre recombinase with PGK Promoter (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_CMV::rtTA3-bGHpAMXS_chaining vector with CMV::rtTA3-bGHpA cassette containing the reverse tetracycline transactivator with CMV promoter (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_PGK::rtTA3-bGHpAMXS_chaining vector with PGK::rtTA3-bGHpA cassette containing the reverse tetracycline transactivator with PGK promoter (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_CMV::tTA2-bGHpAMXS_chaining vector with CMV::tTA2-bGHpA cassette containing the tetracycline transactivator with CMV promoter (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
MXS_PGK::tTA2-bGHpAMXS_chaining vector with PGK::tTA2-bGHpA cassette containing the tetracycline transactivator with PGK promoter (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
ColorfulCellMXS_chaining vector with 6 cassettes CMV::GTS-Citrine-bGHpA (Synthetic), CMV::Lyn-Ceruleanx3-bGHpA (Synthetic), CMV::NLS-TagBFPx3-bGHpA (Synthetic), CMV::MTS-AzamiGreen-bGHpA (Synthetic), CMV::iRFP670-SKL-bGHpA (Synthetic), CMV::ETS-mCherry-KDEL-bGHpA (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
ChromosomesMembranesandCytoskeletonMXS_chaining vector with 4 cassettes CMV::H2B-TagBFPx3-bGHpA (Synthetic), CMV::Lyn-Ceruleanx3-bGHpA (Synthetic), CMV::mCherryx3-linker-betaActin-bGHpA (Synthetic), CMV::Citrinex3-linker-alphaTubulin-bGHpA (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
ES-FUCCISMX_DEST vector with 3 cassettesminPGK::Citrine-Geminin(1-110)-bGHpA (PGKEnhancer)x5 (Synthetic), minPGK::Cherry-hCdt1(30-120)-bGHpA (Synthetic), PGK::HygroR-bGpA (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
rtTA and TagBFP cassettesMXS_chaining vector with 2 cassettes CMV::NLS-TagBFPx3-bGHpA (Synthetic), CMV::rtTA3-bGHpA (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
EmptyTranscriptionalReporterMXS_chaining vector with 2 cassettes CMVmin::NLS-Citrine-bGHpA (Synthetic), CMV::NLS-Cherry-bGHpA (Synthetic)Synthetic Biology Neveu MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
SP-dCas9-VPRSP-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; mammalian vectorSP-dCas9-VPR (Homo sapiens)Mammalian Expression, CRISPR, Synthetic Biology Church Highly efficient Cas9-mediated transcriptional programming. Nat Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312.
M_ST1n_VPRST1-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; mammalian vectorST1-dCas9-VPR (Homo sapiens)Mammalian Expression, CRISPR, Synthetic Biology Church Highly efficient Cas9-mediated transcriptional programming. Nat Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312.
PB-TRE-dCas9-VPRSP-dCas9-VPR with doxycycline-inducible expressiondCas9-VPR (Homo sapiens)Mammalian Expression, CRISPR, Synthetic Biology ; Piggybac Church Highly efficient Cas9-mediated transcriptional programming. Nat Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312.
pSB700Lentiviral vector for expressing U6 sgRNA and CAGGS Cerulean fluorescent proteinMammalian Expression, Lentiviral, CRISPR, Synthetic Biology Church Highly efficient Cas9-mediated transcriptional programming. Nat Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312.
pJZC42sgRNA + 1XPP7 with PCP-VP64 effector for mammalian cells, marked by mCherrysgRNA + 1XPP7, PCP-VP64 IRES mCherryMammalian Expression Lim Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
pJZC43sgRNA + 2XPP7 with PCP-VP64 effector for mammalian cells, marked by mCherrysgRNA + 2XPP7, PCP-VP64 IRES mCherryMammalian Expression Lim Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
Dazl-CPG-TVa targeting vector, to target the endogenous DAZL CPG island with Snrp-GFP-polyA5'hDAZL-snrp-GFP-polyA-pgK-PURO-3'hDAZL (Synthetic)Mouse Targeting, Synthetic Biology Jaenisch Tracing Dynamic Changes of DNA Methylation at Single-Cell Resolution. Cell. 2015 Sep 24;163(1):218-29. doi: 10.1016/j.cell.2015.08.046.
GAPDH-CPG-TVa targeting vector, to target the endogenous Gapdh CPG island with Snrp-GFP-polyA5'hGapdh-Snrp-GFP-polyA-PGK-PURO-3'hGapdh (Synthetic)Mouse Targeting, Synthetic Biology Jaenisch Tracing Dynamic Changes of DNA Methylation at Single-Cell Resolution. Cell. 2015 Sep 24;163(1):218-29. doi: 10.1016/j.cell.2015.08.046.
SOX2se-CPG-TVa targeting vector to target the endogenous Sox2 SE CPG island with Snrp-Tomato-polyA5'hSOX2se-Snrp-Tomato-polyA-Pgk-PURO-3'hSOX2se (Synthetic)Mouse Targeting, Synthetic Biology Jaenisch Tracing Dynamic Changes of DNA Methylation at Single-Cell Resolution. Cell. 2015 Sep 24;163(1):218-29. doi: 10.1016/j.cell.2015.08.046.
pAWp7pFUGW-UBCp-RFP-CMVp-GFPUBCp-RFP-CMVp-GFPLentiviral Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWp11pFUGW-CMVpLentiviral Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWp12pFUGW-CMVp-GFPCMVp-GFPLentiviral Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWp7-1pFUGW-UBCp-RFP-CMVp-GFP-[miR-124]UBCp-RFP-CMVp-GFP-[miR-124]Lentiviral Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWp7-2pFUGW-UBCp-RFP-CMVp-GFP-[miR-128]UBCp-RFP-CMVp-GFP-[miR-128]Lentiviral Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWp7-3pFUGW-UBCp-RFP-CMVp-GFP-[miR-132]UBCp-RFP-CMVp-GFP-[miR-132]Lentiviral Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWp7-5pFUGW-UBCp-RFP-CMVp-GFP-[miR-128 + miR-132]UBCp-RFP-CMVp-GFP-[miR-128 + miR-132]Lentiviral Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWp7-6pFUGW-UBCp-RFP-CMVp-GFP-[miR-124 + miR-128 + miR-132]UBCp-RFP-CMVp-GFP-[miR-124 + miR-128 + miR-132]Lentiviral Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWp7-7pFUGW-UBCp-RFP-[miR-124 sensor]-CMVp-GFPUBCp-RFP-[miR-124 sensor]-CMVp-GFPLentiviral Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWp7-9pFUGW-UBCp-RFP-[miR-132 sensor]-CMVp-GFPUBCp-RFP-[miR-132 sensor]-CMVp-GFPLentiviral Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWp7-10pFUGW-UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-124]UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-124]Lentiviral Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWp7-11pFUGW-UBCp-RFP-[miR-128 sensor]-CMVp-GFP-[miR-124]UBCp-RFP-[miR-128 sensor]-CMVp-GFP-[miR-124]Lentiviral Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWp7-12pFUGW-UBCp-RFP-[miR-132 sensor]-CMVp-GFP-[miR-124]UBCp-RFP-[miR-132 sensor]-CMVp-GFP-[miR-124]Lentiviral Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWp7-13pFUGW-UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-128]UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-128]Lentiviral Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWp7-14pFUGW-UBCp-RFP-[miR-128 sensor]-CMVp-GFP-[miR-128]UBCp-RFP-[miR-128 sensor]-CMVp-GFP-[miR-128]Lentiviral Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWp7-15pFUGW-UBCp-RFP-[miR-132 sensor]-CMVp-GFP-[miR-128]UBCp-RFP-[miR-132 sensor]-CMVp-GFP-[miR-128]Lentiviral Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWp7-16pFUGW-UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-132]UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-132]Lentiviral Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWp7-17pFUGW-UBCp-RFP-[miR-128 sensor]-CMVp-GFP-[miR-132]UBCp-RFP-[miR-128 sensor]-CMVp-GFP-[miR-132]Lentiviral Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWp7-18pFUGW-UBCp-RFP-[miR-132 sensor]-CMVp-GFP-[miR-132]UBCp-RFP-[miR-132 sensor]-CMVp-GFP-[miR-132]Lentiviral Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWp7-19pFUGW-UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-124 + miR-128]UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-124 + miR-128]Lentiviral Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWp7-20pFUGW-UBCp-RFP-[miR-128 sensor]-CMVp-GFP-[miR-128 + miR-132]UBCp-RFP-[miR-128 sensor]-CMVp-GFP-[miR-128 + miR-132]Lentiviral Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-1pBT264-[let-7a][let-7a] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-2pBT264-[let-7b/miR-4763 cluster][let-7b/miR-4763 cluster] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-3pBT264-[let-7c][let-7c] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-5pBT264-[let-7e/miR-99b cluster][let-7e/miR-99b cluster] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-7pBT264-[miR-10b][miR-10b] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-8pBT264-[miR-126][miR-126] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-9pBT264-[miR-128b][miR-128b] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-10pBT264-[miR-129-2][miR-129-2] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-11pBT264-[miR-132][miR-132] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-12pBT264-[miR-15b/16-2 cluster][miR-15b/16-2 cluster] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-13pBT264-[miR-16-1/15a cluster][miR-16-1/15a cluster] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-14pBT264-[miR-181a][miR-181a] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-15pBT264-[miR-181c][miR-181c] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-16pBT264-[miR-188][miR-188] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-17pBT264-[miR-196][miR-196] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-18pBT264-[miR-199b/3154 cluster][miR-199b/3154 cluster] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-19pBT264-[miR-211][miR-211] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-20pBT264-[miR-212][miR-212] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-21pBT264-[miR-216][miR-216] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-22pBT264-[miR-24-2/27a/23a cluster][miR-24-2/27a/23a cluster] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-24pBT264-[miR-29b][miR-29b] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-25pBT264-[miR-29c][miR-29c] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-26pBT264-[miR-31][miR-31] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-28pBT264-[miR-328][miR-328] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-29pBT264-[miR-34a][miR-34a] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-30pBT264-[miR-373][miR-373] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-31pBT264-[miR-376a][miR-376a] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-32pBT264-[miR-429][miR-429] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-33pBT264-[miR-451/144/4732 cluster][miR-451/144/4732 cluster] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-34pBT264-[miR-488][miR-488] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-35pBT264-[miR-489][miR-489] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-36pBT264-[miR-708][miR-708] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-37pBT264-[miR-9-1][miR-9-1] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-38pBT264-[miR-93/106b cluster][miR-93/106b cluster] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pBP-TL3RBS_H3H3 RBS (Synthetic)Synthetic Biology Freemont EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth Biol. 2016 May 2.
pTriEx-mCherry-LINXa3Light-induced nuclear export switch for microscopy in mammalian cells.LINXa3 (Synthetic)Mammalian Expression, Bacterial Expression, Insect Expression Kuhlman Light-induced nuclear export reveals rapid dynamics of epigenetic modifications. Nat Chem Biol. 2016 Jun;12(6):399-401. doi: 10.1038/nchembio.2068. Epub 2016 Apr 18.
pTriEx-mCherry-LINXb3Light-induced nuclear export switch for microscopy in mammalian cells.LINXb3 (Synthetic)Mammalian Expression, Bacterial Expression, Insect Expression Kuhlman Light-induced nuclear export reveals rapid dynamics of epigenetic modifications. Nat Chem Biol. 2016 Jun;12(6):399-401. doi: 10.1038/nchembio.2068. Epub 2016 Apr 18.
pTriEx-mCherry-LINXa3-nanoLight-induced nuclear export switch fusion to nano for microscopy in mammalian cells.LINXa3-nano (Synthetic)Mammalian Expression, Bacterial Expression, Insect Expression Kuhlman Light-induced nuclear export reveals rapid dynamics of epigenetic modifications. Nat Chem Biol. 2016 Jun;12(6):399-401. doi: 10.1038/nchembio.2068. Epub 2016 Apr 18.
Flamindo2Expresses biosensor for cAMP in mammalian cellsFlamindo2Mammalian Expression Kitaguchi Genetically-encoded yellow fluorescent cAMP indicator with an expanded dynamic range for dual-color imaging. PLoS One. 2014 Jun 24;9(6):e100252. doi: 10.1371/journal.pone.0100252. eCollection 2014.
nls-Flamindo2Expresses biosensor for cAMP in mammalian cells with nuclear localization signalnls-Flamindo2Mammalian Expression Kitaguchi Genetically-encoded yellow fluorescent cAMP indicator with an expanded dynamic range for dual-color imaging. PLoS One. 2014 Jun 24;9(6):e100252. doi: 10.1371/journal.pone.0100252. eCollection 2014.
mRFP-Lact-C2Fluorescent biosensor for phosphatidylserine.Lactadherin-C2 (Bos taurus)Mammalian Expression Grinstein Membrane phosphatidylserine regulates surface charge and protein localization. Science. 2008 Jan 11. 319(5860):210-3.
PhiC31-Neo-ins-5xTetO-pEF-H2B-Citrine-insNuclear fluorescent reporter in mammalian cells, phiC31 attB for integration, can be silenced with TetR-CR or rTetR-CR (e.g. CR can be KRAB or DNMT3B).Histone H2B - Citrine (Homo sapiens)Mammalian Expression, Synthetic Biology Elowitz Dynamics of epigenetic regulation at the single-cell level. Science. 2016 Feb 12;351(6274):720-4. doi: 10.1126/science.aab2956.
pEx1-pEF-H2B-mCherry-T2A-rTetR-EEDExpresses rTetR-EED for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.H2B-mCherry-T2A-rTetR-EED (Homo sapiens)Mammalian Expression, Synthetic Biology Elowitz Dynamics of epigenetic regulation at the single-cell level. Science. 2016 Feb 12;351(6274):720-4. doi: 10.1126/science.aab2956.
MXS_BidirectionalCAGBidirectional CAG promoter (compatible with the MXS Chaining Kit)Bidirectional CAG Promoter (Synthetic)Mammalian Expression, Synthetic Biology Neveu Bidirectional Promoter Engineering for Single Cell MicroRNA Sensors in Embryonic Stem Cells. PLoS One. 2016 May 6;11(5):e0155177. doi: 10.1371/journal.pone.0155177. eCollection 2016.
pEx1-pEF-H2B-mCherry-T2A-rTetR-KRABExpresses rTetR-KRAB for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.H2B-mCherry-T2A-rTetR-KRAB (Homo sapiens)Mammalian Expression, Synthetic Biology Elowitz Dynamics of epigenetic regulation at the single-cell level. Science. 2016 Feb 12;351(6274):720-4. doi: 10.1126/science.aab2956.
pEx1-pEF-H2B-mCherry-T2A-rTetR-HDAC4Expresses rTetR-HDAC4 for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.H2B-mCherry-T2A-rTetR-HDAC4 (Homo sapiens)Mammalian Expression, Synthetic Biology Elowitz Dynamics of epigenetic regulation at the single-cell level. Science. 2016 Feb 12;351(6274):720-4. doi: 10.1126/science.aab2956.
pEx1-pEF-H2B-mCherry-T2A-rTetR-KRAB-ZeoExpresses rTetR-KRAB for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.H2B-mCherry-T2A-rTetR-KRAB (Homo sapiens)Mammalian Expression, Synthetic Biology Elowitz Dynamics of epigenetic regulation at the single-cell level. Science. 2016 Feb 12;351(6274):720-4. doi: 10.1126/science.aab2956.
pEx1-pEF-H2B-mCherry-T2A-rTetR-HDAC4-ZeoExpresses rTetR-HDAC4 for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.H2B-mCherry-T2A-rTetR-HDAC4 (Homo sapiens)Mammalian Expression, Synthetic Biology Elowitz Dynamics of epigenetic regulation at the single-cell level. Science. 2016 Feb 12;351(6274):720-4. doi: 10.1126/science.aab2956.

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