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SynBio_Icon_Metabolism.png Synthetic Biology: Metabolism


Browse Addgene's collection of synthetic biology plasmids related to metabolic pathways and components.

Examples Include:

  • Biofuels
  • Natural Product Pathways
  • Efflux Pumps
  • Flux Control

Metabolism Plasmids

Search the table by keyword or sort by the table headings. Click on the publication link to view all plasmids available from the article. Click on the PI link to view all plasmids available from this depositor's lab.

Plasmid Gene/Insert Vector Type Promoter PI Publication
pBAD33MevTMevT (Saccharomyces cerevisiae)Bacterial Expression, Synthetic Biology Keasling Engineering a mevalonate pathway in Escherichia coli for production of terpenoids. Nat Biotechnol. 2003 Jul . 21(7):796-802.
pMevT (Saccharomyces cerevisiae)Bacterial Expression, Synthetic Biology Keasling Engineering a mevalonate pathway in Escherichia coli for production of terpenoids. Nat Biotechnol. 2003 Jul . 21(7):796-802.
pMBImevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase (Saccharomyces cerevisiae)Bacterial Expression, Synthetic Biology Keasling Engineering a mevalonate pathway in Escherichia coli for production of terpenoids. Nat Biotechnol. 2003 Jul . 21(7):796-802.
pMBISmevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase, FPP synthase (Saccharomyces cerevisiae)Bacterial Expression, Synthetic Biology Keasling Engineering a mevalonate pathway in Escherichia coli for production of terpenoids. Nat Biotechnol. 2003 Jul . 21(7):796-802.
pMevBmevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse (Saccharomyces cerevisiae)Bacterial Expression, Synthetic Biology Keasling Engineering a mevalonate pathway in Escherichia coli for production of terpenoids. Nat Biotechnol. 2003 Jul . 21(7):796-802.
pBAD24MevTMevT (Saccharomyces cerevisiae)Bacterial Expression, Synthetic Biology Keasling Balancing a heterologous mevalonate pathway for improved isoprenoid production in Escherichia coli. Metab Eng. 2007 Mar . 9(2):193-207.
pBdxsdxsBacterial Expression, Synthetic Biology Keasling Metabolic engineering of the nonmevalonate isopentenyl diphosphate synthesis pathway in Escherichia coli enhances lycopene production. Biotechnol Bioeng. 2001 Feb 20. 72(4):408-15.
pBAD*RFPEC2E. coli optimized red flourescent protein (RFPec2)Bacterial Expression, Synthetic Biology Keasling Optimization of DsRed production in Escherichia coli: effect of ribosome binding site sequestration on translation efficiency. Biotechnol Bioeng. 2005 Dec 5. 92(5):553-8.
pET30-R5-GFPpET30-GFPBacterial Expression, Synthetic Biology Keasling Isotopomer distributions in amino acids from a highly expressed protein as a proxy for those from total protein. Anal Chem. 2008 Feb 1. 80(3):886-90.
pAC-PHYTcrtE, crtBBacterial Expression, Synthetic Biology Keasling Controlling the metabolic flux through the carotenoid pathway using directed mRNA processing and stabilization. Metab Eng. 2001 Oct . 3(4):313-21.
pADScodon-optimized synthetic amorphadiene synthaseBacterial Expression, Synthetic Biology Keasling Engineering a mevalonate pathway in Escherichia coli for production of terpenoids. Nat Biotechnol. 2003 Jul . 21(7):796-802.
pBAD18HMGRtruncated 3-hydroxy-3-methyl-glutaryl-coenzyme A Reductase (Saccharomyces cerevisiae)Bacterial Expression, Synthetic Biology Keasling Balancing a heterologous mevalonate pathway for improved isoprenoid production in Escherichia coli. Metab Eng. 2007 Mar . 9(2):193-207.
pSB77thiosulfate reductaseBacterial Expression, Synthetic Biology Keasling Engineering hydrogen sulfide production and cadmium removal by expression of the thiosulfate reductase gene (phsABC) from Salmonella enterica serovar typhimurium in Escherichia coli. Appl Environ Microbiol. 2000 Sep . 66(9):3939-44.
pSB74thiosulfate reductaseBacterial Expression, Synthetic Biology Keasling Engineering hydrogen sulfide production and cadmium removal by expression of the thiosulfate reductase gene (phsABC) from Salmonella enterica serovar typhimurium in Escherichia coli. Appl Environ Microbiol. 2000 Sep . 66(9):3939-44.
pDUET-ctCPR-nAMOp450 reductase and native Amorphadiene oxidaseBacterial Expression, Synthetic Biology Keasling Engineering Escherichia coli for production of functionalized terpenoids using plant P450s. Nat Chem Biol. 2007 May . 3(5):274-7.
pDUET-ctCPR-sAMOp450 reductase and codon optimized amorphadiene oxidaseBacterial Expression, Synthetic Biology Keasling Engineering Escherichia coli for production of functionalized terpenoids using plant P450s. Nat Chem Biol. 2007 May . 3(5):274-7.
pDUET-ctCPR-trAMOp450 reductase and truncated amorphadiene oxidaseBacterial Expression, Synthetic Biology Keasling Engineering Escherichia coli for production of functionalized terpenoids using plant P450s. Nat Chem Biol. 2007 May . 3(5):274-7.
pDUET-ctCPR-BovAMOp450 reductase, and amorphadiene oxidaseBacterial Expression, Synthetic Biology Keasling Engineering Escherichia coli for production of functionalized terpenoids using plant P450s. Nat Chem Biol. 2007 May . 3(5):274-7.
pDUET-ctCPR-A13AMOp450 reductase and amorphadiene oxidaseBacterial Expression, Synthetic Biology Keasling Engineering Escherichia coli for production of functionalized terpenoids using plant P450s. Nat Chem Biol. 2007 May . 3(5):274-7.
pDUET-ctCPR-A17AMOp450 reductase and amorphadiene oxidaseBacterial Expression, Synthetic Biology Keasling Engineering Escherichia coli for production of functionalized terpenoids using plant P450s. Nat Chem Biol. 2007 May . 3(5):274-7.
pDUET-aaCPRct-A13AMOp450 reductase and Amorphadiene oxidaseBacterial Expression, Synthetic Biology Keasling Engineering Escherichia coli for production of functionalized terpenoids using plant P450s. Nat Chem Biol. 2007 May . 3(5):274-7.
pCWori-A13AMO-aaCPRctAmorphadiene oxidase and p450 reductaseBacterial Expression, Synthetic Biology Keasling Engineering Escherichia coli for production of functionalized terpenoids using plant P450s. Nat Chem Biol. 2007 May . 3(5):274-7.
pESC-URA::CYP71AV1/CPRamorphadiene oxidase and p450 reductaseYeast Expression, Synthetic Biology Keasling Production of the antimalarial drug precursor artemisinic acid in engineered yeast. Nature. 2006 Apr 13. 440(7086):940-3.
pRS425ADSamorphadiene synthaseYeast Expression, Synthetic Biology Keasling Production of the antimalarial drug precursor artemisinic acid in engineered yeast. Nature. 2006 Apr 13. 440(7086):940-3.
pKS2'tesABacterial Expression, Synthetic Biology Keasling Microbial production of fatty-acid-derived fuels and chemicals from plant biomass. Nature. 2010 Jan 28;463(7280):559-62.
pKS13pdc, adhBBacterial Expression, Synthetic Biology Keasling Microbial production of fatty-acid-derived fuels and chemicals from plant biomass. Nature. 2010 Jan 28;463(7280):559-62.
pKS17pdc, adhB, atfABacterial Expression, Synthetic Biology Keasling Microbial production of fatty-acid-derived fuels and chemicals from plant biomass. Nature. 2010 Jan 28;463(7280):559-62.
pKS18mFar1 (Mus musculus)Bacterial Expression, Synthetic Biology Keasling Microbial production of fatty-acid-derived fuels and chemicals from plant biomass. Nature. 2010 Jan 28;463(7280):559-62.
pKS100fadDBacterial Expression, Synthetic Biology Keasling Microbial production of fatty-acid-derived fuels and chemicals from plant biomass. Nature. 2010 Jan 28;463(7280):559-62.
pKS101FAA2 (Saccharomyces cerevisiae)Bacterial Expression, Synthetic Biology Keasling Microbial production of fatty-acid-derived fuels and chemicals from plant biomass. Nature. 2010 Jan 28;463(7280):559-62.
pKS104fadD (M3351), atfABacterial Expression, Synthetic Biology Keasling Microbial production of fatty-acid-derived fuels and chemicals from plant biomass. Nature. 2010 Jan 28;463(7280):559-62.
pKS1'tesABacterial Expression, Synthetic Biology Keasling Microbial production of fatty-acid-derived fuels and chemicals from plant biomass. Nature. 2010 Jan 28;463(7280):559-62.
pGB3xynBBacterial Expression, Synthetic Biology Keasling Microbial production of fatty-acid-derived fuels and chemicals from plant biomass. Nature. 2010 Jan 28;463(7280):559-62.
pBbA5c-MevT-MBISAtoB(o)-HMGS(o)-HMGR(o)-MK(o)-PMK(o)-PMD-Idi-IspA (Saccharomyces cerevisiae)Bacterial Expression, Synthetic BiologyLacUV5, Lee Identification and microbial production of a terpene-based advanced biofuel. Nat Commun. 2011 Sep 27;2:483. doi: 10.1038/ncomms1494.
pBbA5c-MevT(CO)-MBIS(CO, ispA)AtoB(co)-HMGS(co)-HMGR(co)-MK(co)-PMK(co)-PMD-Idi-IspA (Saccharomyces cerevisiae)Bacterial Expression, Synthetic BiologyLacUV5, Lee Identification and microbial production of a terpene-based advanced biofuel. Nat Commun. 2011 Sep 27;2:483. doi: 10.1038/ncomms1494.
pBbA5c-MevT(CO)-T1-MBIS(CO, ispA)AtoB(co)-HMGS(co)-HMGR(co)-pTrc-MK(co)-PMK(co)-PMD-Idi-IspA (Saccharomyces cerevisiae)Bacterial Expression, Synthetic BiologyLacUV5, pTrc Lee Identification and microbial production of a terpene-based advanced biofuel. Nat Commun. 2011 Sep 27;2:483. doi: 10.1038/ncomms1494.
pTrcAgBIS (CO)Bisabolene SynthaseBacterial Expression, Synthetic Biology pTrc Lee Identification and microbial production of a terpene-based advanced biofuel. Nat Commun. 2011 Sep 27;2:483. doi: 10.1038/ncomms1494.
pRSLeu2d-BISoptBisabolene SynthaseYeast Expression, Synthetic BiologyP GAL 1 Lee Identification and microbial production of a terpene-based advanced biofuel. Nat Commun. 2011 Sep 27;2:483. doi: 10.1038/ncomms1494.
pAC-4CL14-coumaroyl CoA ligase 1 (Arabidopsis thaliana)Bacterial Expression, Synthetic BiologypLac-mod Schmidt-dannert Biosynthesis of plant-specific stilbene polyketides in metabolically engineered Escherichia coli. BMC Biotechnol. 2006 Mar 21;6:22.
pUC-STSStilbene synthaseSynthetic BiologyLacPMod Schmidt-dannert Biosynthesis of plant-specific stilbene polyketides in metabolically engineered Escherichia coli. BMC Biotechnol. 2006 Mar 21;6:22.
pCellulosepCelluloseSynthetic BiologyTwo promoters from the E coli genome drive two different genes: PcspD and PwrbA Keasling Synthesis of three advanced biofuels from ionic liquid-pretreated switchgrass using engineered Escherichia coli. Proc Natl Acad Sci U S A. 2011 Dec 13;108(50):19949-54. Epub 2011 Nov 28.
pXylanpXylanSynthetic BiologyTwo promoters from the E coli genome drive two different genes: PcspD and PcstA Keasling Synthesis of three advanced biofuels from ionic liquid-pretreated switchgrass using engineered Escherichia coli. Proc Natl Acad Sci U S A. 2011 Dec 13;108(50):19949-54. Epub 2011 Nov 28.
pBA2kIKmA2Kudzu Isoprene Synthase (Synthetic)Synthetic BiologypsbA2 Melis Engineering a platform for photosynthetic isoprene production in cyanobacteria, using Synechocystis as the model organism. Metab Eng. 2010 Jan;12(1):70-9. Epub 2009 Oct 13.
pBA2SkIKmA2Synechocystis kudzu IspSSynthetic BiologypsbA2 Melis Engineering a platform for photosynthetic isoprene production in cyanobacteria, using Synechocystis as the model organism. Metab Eng. 2010 Jan;12(1):70-9. Epub 2009 Oct 13.
pAM1518psbAI promoter, luxCDECyanobacteria cloning vectorn/a Golden Application of bioluminescence to the study of circadian rhythms in cyanobacteria. Methods Enzymol. 2000;305:527-42.
pBbA5k-EPL123AcrABBacterial Expression, Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL3mexF, Avin_33870 (Synthetic)Bacterial Expression, Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL4Avin_44060 (Synthetic)Bacterial Expression, Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL11ttgB, PP_1385 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL12PP_3456 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL14mexF, PP_3426 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL15PP_0906 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL33PA_2018 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL36mexF, PA2494 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL37PA_4375 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL42PFL_1331 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL43PFL_3271 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL45PFL_1028 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL48PFL_1158 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL52Rmet_4866 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL54Rmet_1702 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL55Maqu_3494 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL56Maqu_0582 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL59PSPPH_4014 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL61PSPPH_2907 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL63mdtB, PSPPH_2641 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL64PSPPH_0733 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL65PSPPH_1196 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL66PSPPH_0329Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL68Gmet_1664 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL69Gmet_2518 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL70Gmet_1652 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL84BP_2076 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL90DVU_0438 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL95AcrB/AcrD/AcrFa, ABO_0964 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL98acrB, PSHAb_0324 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL100Shal_0198 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL101Shal_1195 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL102Shal_2167 (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL122acrF (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pBbA5k-EPL124acrD (Synthetic)Synthetic BiologyLacUV5 Mukhopadhyay Engineering microbial biofuel tolerance and export using efflux pumps. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
pJBEI-6409Codon-optimized sequences for MEV pathway expression in E. coli to produce limonene (Saccharomyces cerevisiae)Bacterial Expression, Synthetic Biology ; BglBrickPlacUV5 Lee Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production. Metab Eng. 2013 May 29;19C:33-41. doi: 10.1016/j.ymben.2013.05.004.
pJBEI-6410Codon-optimized sequences for MEV pathway expression in E. coli to produce limonene (Saccharomyces cerevisiae)Bacterial Expression, Synthetic Biology ; BglBrickPlacUV5 Lee Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production. Metab Eng. 2013 May 29;19C:33-41. doi: 10.1016/j.ymben.2013.05.004.
pJBEI-6411ahpG-ahpH-ahpI coding for cytochrome P450 (CYP153A6) and its associated ferredoxin and ferredoxin reductase (Other)Bacterial Expression, Synthetic Biology ; BglBrickPbad Lee Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production. Metab Eng. 2013 May 29;19C:33-41. doi: 10.1016/j.ymben.2013.05.004.
pS3aroE, aroD, aroB, aroG*, ppsA, tktABacterial Expression, Synthetic BiologylacUV5, lacUV5, lacUV5, lacUV5, lacUV5, lacUV5 Keasling Modular engineering of L-tyrosine production in Escherichia coli. Appl Environ Microbiol. 2012 Jan;78(1):89-98. doi: 10.1128/AEM.06017-11. Epub 2011 Oct 21.
pY1noneBacterial Expression, Synthetic Biology ; Empty VectorPLacUV5 Keasling Modular engineering of L-tyrosine production in Escherichia coli. Appl Environ Microbiol. 2012 Jan;78(1):89-98. doi: 10.1128/AEM.06017-11. Epub 2011 Oct 21.
pTrc-trGPPS(CO)-LSLimonene Synthase, ApR, LacIqBacterial Expression, Synthetic BiologyTrc, Trc, Trc Keasling Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production. Metab Eng. 2013 May 29;19C:33-41. doi: 10.1016/j.ymben.2013.05.004.
pY3tyrB, tyrA, aroC, aroA, aroLBacterial Expression, Synthetic BiologylacUV5, lacUV5, lacUV5, lacUV5, lacUV5 Keasling Modular engineering of L-tyrosine production in Escherichia coli. Appl Environ Microbiol. 2012 Jan;78(1):89-98. doi: 10.1128/AEM.06017-11. Epub 2011 Oct 21.
pET-Ce25arginyl-tRNA synthetase (Other)Bacterial ExpressionT7 Igloi Expression and properties of arginyl-tRNA synthetase from jack bean (Canavalia ensiformis). Protein Expr Purif. 2008 Oct;61(2):163-7. doi: 10.1016/j.pep.2008.05.008. Epub 2008 May 23.
psbA2-PHLS (b)beta-phellandrene synthase (Other), Chloramphenicol resistanceBacterial Expression, Synthetic BiologypsbA2 Melis Regulation of β-phellandrene synthase gene expression, recombinant protein accumulation, and monoterpene hydrocarbons production in Synechocystis transformants. Planta May 2014
psbA2(noATbox)-PHLS (c)beta-phellandrene synthase (Other), Chloramphenicol resistanceBacterial Expression, Synthetic BiologypsbA2 Melis Regulation of β-phellandrene synthase gene expression, recombinant protein accumulation, and monoterpene hydrocarbons production in Synechocystis transformants. Planta May 2014
psbA2-Ptrc-PHLS (d)beta-phellandrene synthase (Other), Chloramphenicol resistanceBacterial Expression, Synthetic BiologyPtrc Melis Regulation of β-phellandrene synthase gene expression, recombinant protein accumulation, and monoterpene hydrocarbons production in Synechocystis transformants. Planta May 2014
psbA2-Ptrc-T7-g10-PHLS (e)beta-phellandrene synthase (Other), Chloramphenicol resistanceBacterial Expression, Synthetic BiologyPtrc-T7 Melis Regulation of β-phellandrene synthase gene expression, recombinant protein accumulation, and monoterpene hydrocarbons production in Synechocystis transformants. Planta May 2014
cpc-PHLS (g)beta-phellandrene synthase (Other), Chloramphenicol resistanceBacterial Expression, Synthetic BiologySynechocystis endogenous cpc-operon promoter Melis Regulation of β-phellandrene synthase gene expression, recombinant protein accumulation, and monoterpene hydrocarbons production in Synechocystis transformants. Planta May 2014
pNtCp-rbcL-PHLS-aadA-accDrbcL-PHLS-aadA-accD (Other)Bacterial Expression, Synthetic Biology Melis Melis lab pNtCp-rbcL-accD (unpublished)
pNtCp-rbcL-PHLS-GPPS-aadA-accDrbcL-PHLS-GPPS-aadA-accD (Other)Bacterial Expression, Synthetic Biology Melis Melis lab pNtCp-rbcL-accD (unpublished)
pET-Gm1arginyl-tRNA synthetase (Other)Bacterial ExpressionT7 Igloi Amino acid discrimination by arginyl-tRNA synthetases as revealed by an examination of natural specificity variants. FEBS J. 2009 Mar;276(5):1307-18. doi: 10.1111/j.1742-4658.2009.06866.x.
pET-Cb2mitochondrial arginyl-tRNA synthetase (Other)Bacterial ExpressionT7 Igloi Amino acid discrimination by the nuclear encoded mitochondrial arginyl-tRNA synthetase of the larva of a bruchid beetle (Caryedes brasiliensis) from northwestern Costa Rica. Insect Biochem Mol Biol. 2013 Dec;43(12):1172-80.
pEU-Gm23arginyl-tRNA synthetase (Other)Synthetic BiologySP6 Igloi Wheat germ in vitro translation of soybean arginyl-tRNA synthetase (unpublished)
pBbE1k-2dihydropterine reductase, pterin-4a-carbinolamine dehydratasepTrc, pTrc Lee Engineering of L-tyrosine oxidation in Escherichia coli and microbial production of hydroxytyrosol. Metab Eng. 2012 Nov;14(6):603-10. doi: 10.1016/j.ymben.2012.08.002. Epub 2012 Aug 29.
pBAMD1-2Synthetic Biology ; Mini-Tn5 vector de Lorenzo New transposon tools tailored for metabolic engineering of gram-negative microbial cell factories. Front Bioeng Biotechnol. 2014 Oct 28;2:46. doi: 10.3389/fbioe.2014.00046. eCollection 2014.
pBAMD1-4Synthetic Biology ; Mini-Tn5 vector de Lorenzo New transposon tools tailored for metabolic engineering of gram-negative microbial cell factories. Front Bioeng Biotechnol. 2014 Oct 28;2:46. doi: 10.3389/fbioe.2014.00046. eCollection 2014.
pBAMD1-6Synthetic Biology ; Mini-Tn5 vector de Lorenzo New transposon tools tailored for metabolic engineering of gram-negative microbial cell factories. Front Bioeng Biotechnol. 2014 Oct 28;2:46. doi: 10.3389/fbioe.2014.00046. eCollection 2014.
p148flavaone 3B-hydroxylase (Other), dihydroflavonol 4-reductase (Other), leucoanthocyanidin reductase (Other)Bacterial Expression, Synthetic BiologyT7, T7, T7 Koffas Improvement of catechin production in Escherichia coli through combinatorial metabolic engineering. Metab Eng. 2014 Dec 17;28C:43-53. doi: 10.1016/j.ymben.2014.12.002.
p158flavaone 3B-hydroxylase (Other), dihydroflavonol 4-reductase (Other), leucoanthocyanidin reductase (Other)Bacterial Expression, Synthetic BiologyT7, T7, T7 Koffas Improvement of catechin production in Escherichia coli through combinatorial metabolic engineering. Metab Eng. 2014 Dec 17;28C:43-53. doi: 10.1016/j.ymben.2014.12.002.
p168flavaone 3B-hydroxylase (Other), dihydroflavonol 4-reductase (Other), leucoanthocyanidin reductase (Other)Bacterial Expression, Synthetic BiologyT7, T7, T7 Koffas Improvement of catechin production in Escherichia coli through combinatorial metabolic engineering. Metab Eng. 2014 Dec 17;28C:43-53. doi: 10.1016/j.ymben.2014.12.002.
p168-759flavaone 3B-hydroxylase (Other), dihydroflavonol 4-reductase (Other), leucoanthocyanidin reductase (Other), Scaffold Protein encoding GBD:SH3:PDZ (1:1:4) (Synthetic)Bacterial Expression, Synthetic BiologyT7, T7, T7, T7 Koffas Improvement of catechin production in Escherichia coli through combinatorial metabolic engineering. Metab Eng. 2014 Dec 17;28C:43-53. doi: 10.1016/j.ymben.2014.12.002.
p1585588flavaone 3B-hydroxylase (Other), dihydroflavonol 4-reductase (Other), leucoanthocyanidin reductase (Other), dihydroflavonol 4-reductase (Other), dihydroflavonol 4-reductase (Other), leucoanthocyanidin reductase (Other), leucoanthocyanidin reductase (Other)Bacterial Expression, Synthetic BiologyT7, T7, T7, T7, T7, T7, T7 Koffas Improvement of catechin production in Escherichia coli through combinatorial metabolic engineering. Metab Eng. 2014 Dec 17;28C:43-53. doi: 10.1016/j.ymben.2014.12.002.
LAMP1-ABKARABKAR biosensorMammalian ExpressionCMV Inoue Compartmentalized AMPK Signaling Illuminated by Genetically Encoded Molecular Sensors and Actuators Cell Reports
pDCAF3-IS5ndmA (Other), ndmB (Other), ndmC (Other), ndmD (Other), gst9 (Other), chlR (Other)Bacterial Expression, Synthetic BiologyBBa_J23100, BBa_J23100, BBa_J23100, BBa_J23100, BBa_J23100 Barrick Decaffeination and measurement of caffeine content by addicted Escherichia coli with a refactored N-demethylation operon from Pseudomonas putida CBB5. ACS Synth Biol. 2013 Jun 21;2(6):301-7. doi: 10.1021/sb4000146. Epub 2013 Mar 22.
HcKan_PRFP (Other)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
HcKan_ORFP (Other)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
HcKan_TRFP (Other)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
POT2-RFPRFP (Other)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
POT3-RFPRFP (Other)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
POT4-RFPRFP (Other)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
POT5-RFPRFP (Other)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
POT6-RFPRFP (Other)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
POT7-RFPRFP (Other)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
POT8-RFPRFP (Other)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
POT9-RFPRFP (Other)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
POT10-RFPRFP (Other)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
POT11-RFPRFP (Other)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
pRS414-URRURR1-RFP-Leu2-URR2 (Synthetic)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
pRS424-URRURR1-RFP-Leu2-URR2 (Synthetic)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
pMV-URR1URR1 (Synthetic)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
pMV-URR2URR2 (Synthetic)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
pMV-HIS3HIS3 (Saccharomyces cerevisiae)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
pMV-LEU2LEU2 (Saccharomyces cerevisiae)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
pMV-TRP1TRP1 (Saccharomyces cerevisiae)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
HCKan-P-TDH3TDH3 promoter (Saccharomyces cerevisiae)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
HCKan-P-TEF2TEF2 promoter (Saccharomyces cerevisiae)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
HCKan-P-CYC1CYC1 promoter (Saccharomyces cerevisiae)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
HCKan-T-ADH1ADH1 terminator (Saccharomyces cerevisiae)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
HCKan-T-TEF2TEF2 terminator (Saccharomyces cerevisiae)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
PMV-ycrtEycrtE (Synthetic)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
PMV-ycrtIycrtI (Synthetic)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
PMV-ycrtYBycrtYB (Synthetic)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
HCKan-O-ycrtEycrtE (Synthetic)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
HCKan-O-ycrtIycrtI (Synthetic)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
HCKan-O-ycrtYBycrtYB (Synthetic)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
pKMV-VioAvioA (Synthetic)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
pKMV-VioBvioB (Synthetic)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
pKMV-VioCvioC (Synthetic)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
pKMV-VioDvioD (Synthetic)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
pKMV-VioEvioE (Synthetic)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
pKS042clpABacterial Expression, Synthetic Biologytrc Tyo N-Terminal-Based Targeted, Inducible Protein Degradation in Escherichia coli. PLoS One. 2016 Feb 22;11(2):e0149746. doi: 10.1371/journal.pone.0149746. eCollection 2016.
pKS043clpSBacterial Expression, Synthetic Biologytrc Tyo N-Terminal-Based Targeted, Inducible Protein Degradation in Escherichia coli. PLoS One. 2016 Feb 22;11(2):e0149746. doi: 10.1371/journal.pone.0149746. eCollection 2016.
pKS044clpPBacterial Expression, Synthetic Biologytrc Tyo N-Terminal-Based Targeted, Inducible Protein Degradation in Escherichia coli. PLoS One. 2016 Feb 22;11(2):e0149746. doi: 10.1371/journal.pone.0149746. eCollection 2016.
PAS501 (TJF040)FadD with Q338R mutation (Other)Bacterial ExpressionT7/lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS500 (TJF032)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS502 (TJF065)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS503 (TJF066)FadD with V4F, W5L mutations (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS504 (TJF067)FadD with F447S mutation (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS505 (TJF068)FadD with V451A mutation (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS506 (TJF069)FadD D372G (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS507 (TJF070)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS508 (TJF071)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS509 (TJF032 L448S)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS510 (TJF032 G446S)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS511 (TJF032 Y375R)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS512 (TJF032 S377R)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS513 (TJF080)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS514 (TJF081)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS515 (TJF082)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS516 (TJF083)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS517 (TJF084)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS518 (TJF085)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS519 (TJF086)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS520 (TJF087)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS521 (TJF088)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS522 (TJF089)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS523 (TJF090)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS524 (TJF091)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS525 (TJF092)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS526 (TJF093)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS527 (TJF094)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS528 (TJF059)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS529 (TJF072)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS530 (TJF073)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS531 (TJF074)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS532 (TJF075)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS533 (TJF076)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS534 (TJF077)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS535 (TJF078)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
PAS536 (TJF079)FadD (Other)Bacterial ExpressionT7 lacO Silver Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids. PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
pETM6-C4-mCherrymCherry (Synthetic)Bacterial Expression, Synthetic BiologyMutant T7 Promoter - C4 Koffas ePathOptimize: A Combinatorial Approach for Transcriptional Balancing of Metabolic Pathways. Sci Rep. 2015 Jun 11;5:11301. doi: 10.1038/srep11301.
pETM6-H10-mCherrymCherry (Synthetic)Bacterial Expression, Synthetic BiologyMutant T7 Promoter - H10 Koffas ePathOptimize: A Combinatorial Approach for Transcriptional Balancing of Metabolic Pathways. Sci Rep. 2015 Jun 11;5:11301. doi: 10.1038/srep11301.
pETM6-H9-mCherrymCherry (Synthetic)Bacterial Expression, Synthetic BiologyMutant T7 Promoter - H9 Koffas ePathOptimize: A Combinatorial Approach for Transcriptional Balancing of Metabolic Pathways. Sci Rep. 2015 Jun 11;5:11301. doi: 10.1038/srep11301.
pETM6-G6-mCherrymCherry (Synthetic)Bacterial Expression, Synthetic BiologyMutant T7 Promoter - G6 Koffas ePathOptimize: A Combinatorial Approach for Transcriptional Balancing of Metabolic Pathways. Sci Rep. 2015 Jun 11;5:11301. doi: 10.1038/srep11301.
pETM6-mCherrymCherry (Synthetic)Bacterial Expression, Synthetic BiologyConsensus T7 Koffas ePathOptimize: A Combinatorial Approach for Transcriptional Balancing of Metabolic Pathways. Sci Rep. 2015 Jun 11;5:11301. doi: 10.1038/srep11301.
pETM6-vioABECDVioA (Other), VioB (Other), VioE (Other), VioC (Other), VioD (Other)Bacterial Expression, Synthetic BiologyConsensus T7, Consensus T7, Consensus T7, Consensus T7, Consensus T7 Koffas ePathOptimize: A Combinatorial Approach for Transcriptional Balancing of Metabolic Pathways. Sci Rep. 2015 Jun 11;5:11301. doi: 10.1038/srep11301.
pETM6-G6-vioABECDVioA (Other), VioB (Other), VioE (Other), VioC (Other), VioD (Other)Bacterial Expression, Synthetic BiologyMutant T7 Promoter - G6, Mutant T7 Promoter - G6, Mutant T7 Promoter - G6, Mutant T7 Promoter - G6, Mutant T7 Promoter - G6 Koffas ePathOptimize: A Combinatorial Approach for Transcriptional Balancing of Metabolic Pathways. Sci Rep. 2015 Jun 11;5:11301. doi: 10.1038/srep11301.
pETM6-E12-vioABECDVioA (Other), VioB (Other), VioE (Other), VioC (Other), VioD (Other)Bacterial Expression, Synthetic BiologyMutant T7 Promoter - H10, Mutant T7 Promoter - G6, Mutant T7 Promoter - G6, Mutant T7 Promoter - H10, Mutant T7 Promoter - G6 Koffas ePathOptimize: A Combinatorial Approach for Transcriptional Balancing of Metabolic Pathways. Sci Rep. 2015 Jun 11;5:11301. doi: 10.1038/srep11301.
pETM6-M.accABCDaccA (Other), accB (Other), accC (Other), accD (Other)Bacterial Expression, Synthetic BiologyT7, T7, T7, T7 Koffas Modular optimization of multi-gene pathways for fatty acids production in E. coli. Nat Commun. 2013;4:1409. doi: 10.1038/ncomms2425.
pETM6-P.accABCDaccA (Other), accB (Other), accC (Other), accD (Other)Bacterial Expression, Synthetic BiologyT7, T7, T7, T7 Koffas Modular optimization of multi-gene pathways for fatty acids production in E. coli. Nat Commun. 2013;4:1409. doi: 10.1038/ncomms2425.
pWKS1583amyEopt (Synthetic)Bacterial Expression, Synthetic BiologyPtet Smits The Signal Sequence of the Abundant Extracellular Metalloprotease PPEP-1 Can Be Used to Secrete Synthetic Reporter Proteins in Clostridium difficile. ACS Synth Biol. 2016 Jun 23.
pETM6-HpaBCHpaB (Other), HpaC (Other)Bacterial Expression, Synthetic BiologyT7, T7 Koffas "Optimization of naringenin and p-coumaric acid hydroxylation using the native E. coli hydroxylase complex, HpaBC". Biotechnol Prog. 2015 Oct 21. doi: 10.1002/btpr.2185.
pBbE1k-3dihydropterine reductase (Homo sapiens), pterin-4a-carbinolamine dehydratase (Homo sapiens)pTrc, pTrc Lee Engineering of L-tyrosine oxidation in Escherichia coli and microbial production of hydroxytyrosol. Metab Eng. 2012 Nov;14(6):603-10. doi: 10.1016/j.ymben.2012.08.002. Epub 2012 Aug 29.
pBbE1k-DDCdopa decarboxylase (Other)pTrc Lee Engineering of L-tyrosine oxidation in Escherichia coli and microbial production of hydroxytyrosol. Metab Eng. 2012 Nov;14(6):603-10. doi: 10.1016/j.ymben.2012.08.002. Epub 2012 Aug 29.
pJK108delta-12 fatty acid acetylenase (Other)Bacterial ExpressionT7 Kappock Additional fatty acid desaturase and acetylenase constructs (unpublished)
PsbA2-HMGS-HMGR-ATOB-NPTIHMGS-HMGR-ATOB-NPTI (Other)psbA2 Melis Expression of the MVA pathway in cyanobacteria (unpublished)
pETM6-G6-vioABEPG6-vioABE (Other)Bacterial Expression, CRISPR, Synthetic BiologyPG6 (orthogonal T7-lac variant) Koffas Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
pETM6-G6-vioABE-4A6-vioCPG6-vioABE-P4A6-vioC (Other)Bacterial Expression, CRISPR, Synthetic BiologyPG6 and P4A6 (orthogonal T7-lac variants) Koffas Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
pETM6-G6-vioABE-4A6-vioC-3A2-vioDPG6-vioABE-P4A6-vioC-P3A2-vioD (Other)Bacterial Expression, CRISPR, Synthetic BiologyPG6, P4A6, and P3A2 (orthogonal T7-lac variants) Koffas Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
pJKR-H-benMmuconate biosensing apparatus Church Genetically encoded sensors enable real-time observation of metabolite production. Proc Natl Acad Sci U S A. 2016 Feb 8. pii: 201600375.
pZ8-P_dCas9dcas9Bacterial Expression, CRISPR, Synthetic BiologyPropionate inducible promoter (prp) Lu Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi). ACS Synth Biol. 2016 Feb 16.
pZ8-PtacBacterial Expression, Synthetic Biologyptac Lu Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi). ACS Synth Biol. 2016 Feb 16.
pAL-pgi_TsgRNA pgi (T)Bacterial Expression, CRISPR, Synthetic Biologyptac Lu Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi). ACS Synth Biol. 2016 Feb 16.
pAL-pgi_NTsgRNA pgi (NT) (Synthetic)Bacterial Expression, CRISPR, Synthetic Biologyptac Lu Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi). ACS Synth Biol. 2016 Feb 16.
pAL-pck_TsgRNA pck (T) (Synthetic)Bacterial Expression, CRISPR, Synthetic Biologyptac Lu Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi). ACS Synth Biol. 2016 Feb 16.
pAL-pck_NTsgRNA pck (NT) (Synthetic)Bacterial Expression, CRISPR, Synthetic Biologyptac Lu Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi). ACS Synth Biol. 2016 Feb 16.
pAL-pyk_TsgRNA pyk (T) (Synthetic)Bacterial Expression, CRISPR, Synthetic Biologyptac Lu Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi). ACS Synth Biol. 2016 Feb 16.
pAL-pyk_NTsgRNA pyk (NT) (Synthetic)Bacterial Expression, CRISPRptac Lu Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi). ACS Synth Biol. 2016 Feb 16.
pAL-rfp_TsgRNA rfp (T) (Synthetic)Bacterial Expression, Synthetic Biologyptac Lu Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi). ACS Synth Biol. 2016 Feb 16.
PP259GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP257GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP245GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP277GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP190GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP273GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP160GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP155GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP163GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.

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