| pBAD33MevT | MevT (Saccharomyces cerevisiae) | Bacterial Expression, Synthetic Biology | |
Keasling
| Engineering a mevalonate pathway in Escherichia coli for production of terpenoids.
Nat Biotechnol. 2003 Jul . 21(7):796-802.
|
| pMevT | (Saccharomyces cerevisiae) | Bacterial Expression, Synthetic Biology | |
Keasling
| Engineering a mevalonate pathway in Escherichia coli for production of terpenoids.
Nat Biotechnol. 2003 Jul . 21(7):796-802.
|
| pMBI | mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase (Saccharomyces cerevisiae) | Bacterial Expression, Synthetic Biology | |
Keasling
| Engineering a mevalonate pathway in Escherichia coli for production of terpenoids.
Nat Biotechnol. 2003 Jul . 21(7):796-802.
|
| pMBIS | mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase, FPP synthase (Saccharomyces cerevisiae) | Bacterial Expression, Synthetic Biology | |
Keasling
| Engineering a mevalonate pathway in Escherichia coli for production of terpenoids.
Nat Biotechnol. 2003 Jul . 21(7):796-802.
|
| pMevB | mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse (Saccharomyces cerevisiae) | Bacterial Expression, Synthetic Biology | |
Keasling
| Engineering a mevalonate pathway in Escherichia coli for production of terpenoids.
Nat Biotechnol. 2003 Jul . 21(7):796-802.
|
| pBAD24MevT | MevT (Saccharomyces cerevisiae) | Bacterial Expression, Synthetic Biology | |
Keasling
| Balancing a heterologous mevalonate pathway for improved isoprenoid production in Escherichia coli.
Metab Eng. 2007 Mar . 9(2):193-207.
|
| pBdxs | dxs | Bacterial Expression, Synthetic Biology | |
Keasling
| Metabolic engineering of the nonmevalonate isopentenyl diphosphate synthesis pathway in Escherichia coli enhances lycopene production.
Biotechnol Bioeng. 2001 Feb 20. 72(4):408-15.
|
| pBAD*RFPEC2 | E. coli optimized red flourescent protein (RFPec2) | Bacterial Expression, Synthetic Biology | |
Keasling
| Optimization of DsRed production in Escherichia coli: effect of ribosome binding site sequestration on translation efficiency.
Biotechnol Bioeng. 2005 Dec 5. 92(5):553-8.
|
| pET30-R5-GFP | pET30-GFP | Bacterial Expression, Synthetic Biology | |
Keasling
| Isotopomer distributions in amino acids from a highly expressed protein as a proxy for those from total protein.
Anal Chem. 2008 Feb 1. 80(3):886-90.
|
| pAC-PHYT | crtE, crtB | Bacterial Expression, Synthetic Biology | |
Keasling
| Controlling the metabolic flux through the carotenoid pathway using directed mRNA processing and stabilization.
Metab Eng. 2001 Oct . 3(4):313-21.
|
| pADS | codon-optimized synthetic amorphadiene synthase | Bacterial Expression, Synthetic Biology | |
Keasling
| Engineering a mevalonate pathway in Escherichia coli for production of terpenoids.
Nat Biotechnol. 2003 Jul . 21(7):796-802.
|
| pBAD18HMGR | truncated 3-hydroxy-3-methyl-glutaryl-coenzyme A Reductase (Saccharomyces cerevisiae) | Bacterial Expression, Synthetic Biology | |
Keasling
| Balancing a heterologous mevalonate pathway for improved isoprenoid production in Escherichia coli.
Metab Eng. 2007 Mar . 9(2):193-207.
|
| pSB77 | thiosulfate reductase | Bacterial Expression, Synthetic Biology | |
Keasling
| Engineering hydrogen sulfide production and cadmium removal by expression of the thiosulfate reductase gene (phsABC) from Salmonella enterica serovar typhimurium in Escherichia coli.
Appl Environ Microbiol. 2000 Sep . 66(9):3939-44.
|
| pSB74 | thiosulfate reductase | Bacterial Expression, Synthetic Biology | |
Keasling
| Engineering hydrogen sulfide production and cadmium removal by expression of the thiosulfate reductase gene (phsABC) from Salmonella enterica serovar typhimurium in Escherichia coli.
Appl Environ Microbiol. 2000 Sep . 66(9):3939-44.
|
| pDUET-ctCPR-nAMO | p450 reductase and native Amorphadiene oxidase | Bacterial Expression, Synthetic Biology | |
Keasling
| Engineering Escherichia coli for production of functionalized terpenoids using plant P450s.
Nat Chem Biol. 2007 May . 3(5):274-7.
|
| pDUET-ctCPR-sAMO | p450 reductase and codon optimized amorphadiene oxidase | Bacterial Expression, Synthetic Biology | |
Keasling
| Engineering Escherichia coli for production of functionalized terpenoids using plant P450s.
Nat Chem Biol. 2007 May . 3(5):274-7.
|
| pDUET-ctCPR-trAMO | p450 reductase and truncated amorphadiene oxidase | Bacterial Expression, Synthetic Biology | |
Keasling
| Engineering Escherichia coli for production of functionalized terpenoids using plant P450s.
Nat Chem Biol. 2007 May . 3(5):274-7.
|
| pDUET-ctCPR-BovAMO | p450 reductase, and amorphadiene oxidase | Bacterial Expression, Synthetic Biology | |
Keasling
| Engineering Escherichia coli for production of functionalized terpenoids using plant P450s.
Nat Chem Biol. 2007 May . 3(5):274-7.
|
| pDUET-ctCPR-A13AMO | p450 reductase and amorphadiene oxidase | Bacterial Expression, Synthetic Biology | |
Keasling
| Engineering Escherichia coli for production of functionalized terpenoids using plant P450s.
Nat Chem Biol. 2007 May . 3(5):274-7.
|
| pDUET-ctCPR-A17AMO | p450 reductase and amorphadiene oxidase | Bacterial Expression, Synthetic Biology | |
Keasling
| Engineering Escherichia coli for production of functionalized terpenoids using plant P450s.
Nat Chem Biol. 2007 May . 3(5):274-7.
|
| pDUET-aaCPRct-A13AMO | p450 reductase and Amorphadiene oxidase | Bacterial Expression, Synthetic Biology | |
Keasling
| Engineering Escherichia coli for production of functionalized terpenoids using plant P450s.
Nat Chem Biol. 2007 May . 3(5):274-7.
|
| pCWori-A13AMO-aaCPRct | Amorphadiene oxidase and p450 reductase | Bacterial Expression, Synthetic Biology | |
Keasling
| Engineering Escherichia coli for production of functionalized terpenoids using plant P450s.
Nat Chem Biol. 2007 May . 3(5):274-7.
|
| pESC-URA::CYP71AV1/CPR | amorphadiene oxidase and p450 reductase | Yeast Expression, Synthetic Biology | |
Keasling
| Production of the antimalarial drug precursor artemisinic acid in engineered yeast.
Nature. 2006 Apr 13. 440(7086):940-3.
|
| pRS425ADS | amorphadiene synthase | Yeast Expression, Synthetic Biology | |
Keasling
| Production of the antimalarial drug precursor artemisinic acid in engineered yeast.
Nature. 2006 Apr 13. 440(7086):940-3.
|
| pKS2 | 'tesA | Bacterial Expression, Synthetic Biology | |
Keasling
| Microbial production of fatty-acid-derived fuels and chemicals from plant biomass.
Nature. 2010 Jan 28;463(7280):559-62.
|
| pKS13 | pdc, adhB | Bacterial Expression, Synthetic Biology | |
Keasling
| Microbial production of fatty-acid-derived fuels and chemicals from plant biomass.
Nature. 2010 Jan 28;463(7280):559-62.
|
| pKS17 | pdc, adhB, atfA | Bacterial Expression, Synthetic Biology | |
Keasling
| Microbial production of fatty-acid-derived fuels and chemicals from plant biomass.
Nature. 2010 Jan 28;463(7280):559-62.
|
| pKS18 | mFar1 (Mus musculus) | Bacterial Expression, Synthetic Biology | |
Keasling
| Microbial production of fatty-acid-derived fuels and chemicals from plant biomass.
Nature. 2010 Jan 28;463(7280):559-62.
|
| pKS100 | fadD | Bacterial Expression, Synthetic Biology | |
Keasling
| Microbial production of fatty-acid-derived fuels and chemicals from plant biomass.
Nature. 2010 Jan 28;463(7280):559-62.
|
| pKS101 | FAA2 (Saccharomyces cerevisiae) | Bacterial Expression, Synthetic Biology | |
Keasling
| Microbial production of fatty-acid-derived fuels and chemicals from plant biomass.
Nature. 2010 Jan 28;463(7280):559-62.
|
| pKS104 | fadD (M3351), atfA | Bacterial Expression, Synthetic Biology | |
Keasling
| Microbial production of fatty-acid-derived fuels and chemicals from plant biomass.
Nature. 2010 Jan 28;463(7280):559-62.
|
| pKS1 | 'tesA | Bacterial Expression, Synthetic Biology | |
Keasling
| Microbial production of fatty-acid-derived fuels and chemicals from plant biomass.
Nature. 2010 Jan 28;463(7280):559-62.
|
| pGB3 | xynB | Bacterial Expression, Synthetic Biology | |
Keasling
| Microbial production of fatty-acid-derived fuels and chemicals from plant biomass.
Nature. 2010 Jan 28;463(7280):559-62.
|
| pBbA5c-MevT-MBIS | AtoB(o)-HMGS(o)-HMGR(o)-MK(o)-PMK(o)-PMD-Idi-IspA (Saccharomyces cerevisiae) | Bacterial Expression, Synthetic Biology | LacUV5, |
Lee
| Identification and microbial production of a terpene-based advanced biofuel.
Nat Commun. 2011 Sep 27;2:483. doi: 10.1038/ncomms1494.
|
| pBbA5c-MevT(CO)-MBIS(CO, ispA) | AtoB(co)-HMGS(co)-HMGR(co)-MK(co)-PMK(co)-PMD-Idi-IspA (Saccharomyces cerevisiae) | Bacterial Expression, Synthetic Biology | LacUV5, |
Lee
| Identification and microbial production of a terpene-based advanced biofuel.
Nat Commun. 2011 Sep 27;2:483. doi: 10.1038/ncomms1494.
|
| pBbA5c-MevT(CO)-T1-MBIS(CO, ispA) | AtoB(co)-HMGS(co)-HMGR(co)-pTrc-MK(co)-PMK(co)-PMD-Idi-IspA (Saccharomyces cerevisiae) | Bacterial Expression, Synthetic Biology | LacUV5, pTrc |
Lee
| Identification and microbial production of a terpene-based advanced biofuel.
Nat Commun. 2011 Sep 27;2:483. doi: 10.1038/ncomms1494.
|
| pTrcAgBIS (CO) | Bisabolene Synthase | Bacterial Expression, Synthetic Biology | pTrc |
Lee
| Identification and microbial production of a terpene-based advanced biofuel.
Nat Commun. 2011 Sep 27;2:483. doi: 10.1038/ncomms1494.
|
| pRSLeu2d-BISopt | Bisabolene Synthase | Yeast Expression, Synthetic Biology | P GAL 1 |
Lee
| Identification and microbial production of a terpene-based advanced biofuel.
Nat Commun. 2011 Sep 27;2:483. doi: 10.1038/ncomms1494.
|
| pAC-4CL1 | 4-coumaroyl CoA ligase 1 (Arabidopsis thaliana) | Bacterial Expression, Synthetic Biology | pLac-mod |
Schmidt-dannert
| Biosynthesis of plant-specific stilbene polyketides in metabolically engineered Escherichia coli.
BMC Biotechnol. 2006 Mar 21;6:22.
|
| pUC-STS | Stilbene synthase | Synthetic Biology | LacPMod |
Schmidt-dannert
| Biosynthesis of plant-specific stilbene polyketides in metabolically engineered Escherichia coli.
BMC Biotechnol. 2006 Mar 21;6:22.
|
| pCellulose | pCellulose | Synthetic Biology | Two promoters from the E coli genome drive two different genes: PcspD and PwrbA |
Keasling
| Synthesis of three advanced biofuels from ionic liquid-pretreated switchgrass using engineered Escherichia coli.
Proc Natl Acad Sci U S A. 2011 Dec 13;108(50):19949-54. Epub 2011 Nov 28.
|
| pXylan | pXylan | Synthetic Biology | Two promoters from the E coli genome drive two different genes: PcspD and PcstA |
Keasling
| Synthesis of three advanced biofuels from ionic liquid-pretreated switchgrass using engineered Escherichia coli.
Proc Natl Acad Sci U S A. 2011 Dec 13;108(50):19949-54. Epub 2011 Nov 28.
|
| pBA2kIKmA2 | Kudzu Isoprene Synthase (Synthetic) | Synthetic Biology | psbA2 |
Melis
| Engineering a platform for photosynthetic isoprene production in cyanobacteria, using Synechocystis as the model organism.
Metab Eng. 2010 Jan;12(1):70-9. Epub 2009 Oct 13.
|
| pBA2SkIKmA2 | Synechocystis kudzu IspS | Synthetic Biology | psbA2 |
Melis
| Engineering a platform for photosynthetic isoprene production in cyanobacteria, using Synechocystis as the model organism.
Metab Eng. 2010 Jan;12(1):70-9. Epub 2009 Oct 13.
|
| pAM1518 | psbAI promoter, luxCDE | Cyanobacteria cloning vector | n/a |
Golden
| Application of bioluminescence to the study of circadian rhythms in cyanobacteria.
Methods Enzymol. 2000;305:527-42.
|
| pBbA5k-EPL123 | AcrAB | Bacterial Expression, Synthetic Biology | LacUV5 |
Mukhopadhyay
| Engineering microbial biofuel tolerance and export using efflux pumps.
Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
|
| pBbA5k-EPL3 | mexF, Avin_33870 (Synthetic) | Bacterial Expression, Synthetic Biology | LacUV5 |
Mukhopadhyay
| Engineering microbial biofuel tolerance and export using efflux pumps.
Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
|
| pBbA5k-EPL4 | Avin_44060 (Synthetic) | Bacterial Expression, Synthetic Biology | LacUV5 |
Mukhopadhyay
| Engineering microbial biofuel tolerance and export using efflux pumps.
Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
|
| pBbA5k-EPL11 | ttgB, PP_1385 (Synthetic) | Synthetic Biology | LacUV5 |
Mukhopadhyay
| Engineering microbial biofuel tolerance and export using efflux pumps.
Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
|
| pBbA5k-EPL12 | PP_3456 (Synthetic) | Synthetic Biology | LacUV5 |
Mukhopadhyay
| Engineering microbial biofuel tolerance and export using efflux pumps.
Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
|
| pBbA5k-EPL14 | mexF, PP_3426 (Synthetic) | Synthetic Biology | LacUV5 |
Mukhopadhyay
| Engineering microbial biofuel tolerance and export using efflux pumps.
Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
|
| pBbA5k-EPL15 | PP_0906 (Synthetic) | Synthetic Biology | LacUV5 |
Mukhopadhyay
| Engineering microbial biofuel tolerance and export using efflux pumps.
Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
|
| pBbA5k-EPL33 | PA_2018 (Synthetic) | Synthetic Biology | LacUV5 |
Mukhopadhyay
| Engineering microbial biofuel tolerance and export using efflux pumps.
Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
|
| pBbA5k-EPL36 | mexF, PA2494 (Synthetic) | Synthetic Biology | LacUV5 |
Mukhopadhyay
| Engineering microbial biofuel tolerance and export using efflux pumps.
Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
|
| pBbA5k-EPL37 | PA_4375 (Synthetic) | Synthetic Biology | LacUV5 |
Mukhopadhyay
| Engineering microbial biofuel tolerance and export using efflux pumps.
Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
|
| pBbA5k-EPL42 | PFL_1331 (Synthetic) | Synthetic Biology | LacUV5 |
Mukhopadhyay
| Engineering microbial biofuel tolerance and export using efflux pumps.
Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
|
| pBbA5k-EPL43 | PFL_3271 (Synthetic) | Synthetic Biology | LacUV5 |
Mukhopadhyay
| Engineering microbial biofuel tolerance and export using efflux pumps.
Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
|
| pBbA5k-EPL45 | PFL_1028 (Synthetic) | Synthetic Biology | LacUV5 |
Mukhopadhyay
| Engineering microbial biofuel tolerance and export using efflux pumps.
Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
|
| pBbA5k-EPL48 | PFL_1158 (Synthetic) | Synthetic Biology | LacUV5 |
Mukhopadhyay
| Engineering microbial biofuel tolerance and export using efflux pumps.
Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
|
| pBbA5k-EPL52 | Rmet_4866 (Synthetic) | Synthetic Biology | LacUV5 |
Mukhopadhyay
| Engineering microbial biofuel tolerance and export using efflux pumps.
Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
|
| pBbA5k-EPL54 | Rmet_1702 (Synthetic) | Synthetic Biology | LacUV5 |
Mukhopadhyay
| Engineering microbial biofuel tolerance and export using efflux pumps.
Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
|
| pBbA5k-EPL55 | Maqu_3494 (Synthetic) | Synthetic Biology | LacUV5 |
Mukhopadhyay
| Engineering microbial biofuel tolerance and export using efflux pumps.
Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
|
| pBbA5k-EPL56 | Maqu_0582 (Synthetic) | Synthetic Biology | LacUV5 |
Mukhopadhyay
| Engineering microbial biofuel tolerance and export using efflux pumps.
Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
|
| pBbA5k-EPL59 | PSPPH_4014 (Synthetic) | Synthetic Biology | LacUV5 |
Mukhopadhyay
| Engineering microbial biofuel tolerance and export using efflux pumps.
Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
|
| pBbA5k-EPL61 | PSPPH_2907 (Synthetic) | Synthetic Biology | LacUV5 |
Mukhopadhyay
| Engineering microbial biofuel tolerance and export using efflux pumps.
Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
|
| pBbA5k-EPL63 | mdtB, PSPPH_2641 (Synthetic) | Synthetic Biology | LacUV5 |
Mukhopadhyay
| Engineering microbial biofuel tolerance and export using efflux pumps.
Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
|
| pBbA5k-EPL64 | PSPPH_0733 (Synthetic) | Synthetic Biology | LacUV5 |
Mukhopadhyay
| Engineering microbial biofuel tolerance and export using efflux pumps.
Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
|
| pBbA5k-EPL65 | PSPPH_1196 (Synthetic) | Synthetic Biology | LacUV5 |
Mukhopadhyay
| Engineering microbial biofuel tolerance and export using efflux pumps.
Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
|
| pBbA5k-EPL66 | PSPPH_0329 | Synthetic Biology | LacUV5 |
Mukhopadhyay
| Engineering microbial biofuel tolerance and export using efflux pumps.
Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
|
| pBbA5k-EPL68 | Gmet_1664 (Synthetic) | Synthetic Biology | LacUV5 |
Mukhopadhyay
| Engineering microbial biofuel tolerance and export using efflux pumps.
Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
|
| pBbA5k-EPL69 | Gmet_2518 (Synthetic) | Synthetic Biology | LacUV5 |
Mukhopadhyay
| Engineering microbial biofuel tolerance and export using efflux pumps.
Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
|
| pBbA5k-EPL70 | Gmet_1652 (Synthetic) | Synthetic Biology | LacUV5 |
Mukhopadhyay
| Engineering microbial biofuel tolerance and export using efflux pumps.
Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
|
| pBbA5k-EPL84 | BP_2076 (Synthetic) | Synthetic Biology | LacUV5 |
Mukhopadhyay
| Engineering microbial biofuel tolerance and export using efflux pumps.
Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
|
| pBbA5k-EPL90 | DVU_0438 (Synthetic) | Synthetic Biology | LacUV5 |
Mukhopadhyay
| Engineering microbial biofuel tolerance and export using efflux pumps.
Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
|
| pBbA5k-EPL95 | AcrB/AcrD/AcrFa, ABO_0964 (Synthetic) | Synthetic Biology | LacUV5 |
Mukhopadhyay
| Engineering microbial biofuel tolerance and export using efflux pumps.
Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
|
| pBbA5k-EPL98 | acrB, PSHAb_0324 (Synthetic) | Synthetic Biology | LacUV5 |
Mukhopadhyay
| Engineering microbial biofuel tolerance and export using efflux pumps.
Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
|
| pBbA5k-EPL100 | Shal_0198 (Synthetic) | Synthetic Biology | LacUV5 |
Mukhopadhyay
| Engineering microbial biofuel tolerance and export using efflux pumps.
Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
|
| pBbA5k-EPL101 | Shal_1195 (Synthetic) | Synthetic Biology | LacUV5 |
Mukhopadhyay
| Engineering microbial biofuel tolerance and export using efflux pumps.
Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
|
| pBbA5k-EPL102 | Shal_2167 (Synthetic) | Synthetic Biology | LacUV5 |
Mukhopadhyay
| Engineering microbial biofuel tolerance and export using efflux pumps.
Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
|
| pBbA5k-EPL122 | acrF (Synthetic) | Synthetic Biology | LacUV5 |
Mukhopadhyay
| Engineering microbial biofuel tolerance and export using efflux pumps.
Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
|
| pBbA5k-EPL124 | acrD (Synthetic) | Synthetic Biology | LacUV5 |
Mukhopadhyay
| Engineering microbial biofuel tolerance and export using efflux pumps.
Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
|
| pJBEI-6409 | Codon-optimized sequences for MEV pathway expression in E. coli to produce limonene (Saccharomyces cerevisiae) | Bacterial Expression, Synthetic Biology ; BglBrick | PlacUV5 |
Lee
| Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production.
Metab Eng. 2013 May 29;19C:33-41. doi: 10.1016/j.ymben.2013.05.004.
|
| pJBEI-6410 | Codon-optimized sequences for MEV pathway expression in E. coli to produce limonene (Saccharomyces cerevisiae) | Bacterial Expression, Synthetic Biology ; BglBrick | PlacUV5 |
Lee
| Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production.
Metab Eng. 2013 May 29;19C:33-41. doi: 10.1016/j.ymben.2013.05.004.
|
| pJBEI-6411 | ahpG-ahpH-ahpI coding for cytochrome P450 (CYP153A6) and its associated ferredoxin and ferredoxin reductase (Other) | Bacterial Expression, Synthetic Biology ; BglBrick | Pbad |
Lee
| Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production.
Metab Eng. 2013 May 29;19C:33-41. doi: 10.1016/j.ymben.2013.05.004.
|
| pS3 | aroE, aroD, aroB, aroG*, ppsA, tktA | Bacterial Expression, Synthetic Biology | lacUV5, lacUV5, lacUV5, lacUV5, lacUV5, lacUV5 |
Keasling
| Modular engineering of L-tyrosine production in Escherichia coli.
Appl Environ Microbiol. 2012 Jan;78(1):89-98. doi: 10.1128/AEM.06017-11. Epub 2011 Oct 21.
|
| pY1 | none | Bacterial Expression, Synthetic Biology ; Empty Vector | PLacUV5 |
Keasling
| Modular engineering of L-tyrosine production in Escherichia coli.
Appl Environ Microbiol. 2012 Jan;78(1):89-98. doi: 10.1128/AEM.06017-11. Epub 2011 Oct 21.
|
| pTrc-trGPPS(CO)-LS | Limonene Synthase, ApR, LacIq | Bacterial Expression, Synthetic Biology | Trc, Trc, Trc |
Keasling
| Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production.
Metab Eng. 2013 May 29;19C:33-41. doi: 10.1016/j.ymben.2013.05.004.
|
| pY3 | tyrB, tyrA, aroC, aroA, aroL | Bacterial Expression, Synthetic Biology | lacUV5, lacUV5, lacUV5, lacUV5, lacUV5 |
Keasling
| Modular engineering of L-tyrosine production in Escherichia coli.
Appl Environ Microbiol. 2012 Jan;78(1):89-98. doi: 10.1128/AEM.06017-11. Epub 2011 Oct 21.
|
| pET-Ce25 | arginyl-tRNA synthetase (Other) | Bacterial Expression | T7 |
Igloi
| Expression and properties of arginyl-tRNA synthetase from jack bean (Canavalia ensiformis).
Protein Expr Purif. 2008 Oct;61(2):163-7. doi: 10.1016/j.pep.2008.05.008. Epub 2008 May 23.
|
| psbA2-PHLS (b) | beta-phellandrene synthase (Other), Chloramphenicol resistance | Bacterial Expression, Synthetic Biology | psbA2 |
Melis
| Regulation of β-phellandrene synthase gene expression, recombinant protein accumulation, and monoterpene hydrocarbons production in Synechocystis transformants.
Planta
May 2014
|
| psbA2(noATbox)-PHLS (c) | beta-phellandrene synthase (Other), Chloramphenicol resistance | Bacterial Expression, Synthetic Biology | psbA2 |
Melis
| Regulation of β-phellandrene synthase gene expression, recombinant protein accumulation, and monoterpene hydrocarbons production in Synechocystis transformants.
Planta
May 2014
|
| psbA2-Ptrc-PHLS (d) | beta-phellandrene synthase (Other), Chloramphenicol resistance | Bacterial Expression, Synthetic Biology | Ptrc |
Melis
| Regulation of β-phellandrene synthase gene expression, recombinant protein accumulation, and monoterpene hydrocarbons production in Synechocystis transformants.
Planta
May 2014
|
| psbA2-Ptrc-T7-g10-PHLS (e) | beta-phellandrene synthase (Other), Chloramphenicol resistance | Bacterial Expression, Synthetic Biology | Ptrc-T7 |
Melis
| Regulation of β-phellandrene synthase gene expression, recombinant protein accumulation, and monoterpene hydrocarbons production in Synechocystis transformants.
Planta
May 2014
|
| cpc-PHLS (g) | beta-phellandrene synthase (Other), Chloramphenicol resistance | Bacterial Expression, Synthetic Biology | Synechocystis endogenous cpc-operon promoter |
Melis
| Regulation of β-phellandrene synthase gene expression, recombinant protein accumulation, and monoterpene hydrocarbons production in Synechocystis transformants.
Planta
May 2014
|
| pNtCp-rbcL-PHLS-aadA-accD | rbcL-PHLS-aadA-accD (Other) | Bacterial Expression, Synthetic Biology | |
Melis
|
Melis lab pNtCp-rbcL-accD
(unpublished)
|
| pNtCp-rbcL-PHLS-GPPS-aadA-accD | rbcL-PHLS-GPPS-aadA-accD (Other) | Bacterial Expression, Synthetic Biology | |
Melis
|
Melis lab pNtCp-rbcL-accD
(unpublished)
|
| pET-Gm1 | arginyl-tRNA synthetase (Other) | Bacterial Expression | T7 |
Igloi
| Amino acid discrimination by arginyl-tRNA synthetases as revealed by an examination of natural specificity variants.
FEBS J. 2009 Mar;276(5):1307-18. doi: 10.1111/j.1742-4658.2009.06866.x.
|
| pET-Cb2 | mitochondrial arginyl-tRNA synthetase (Other) | Bacterial Expression | T7 |
Igloi
| Amino acid discrimination by the nuclear encoded mitochondrial arginyl-tRNA synthetase of the larva of a bruchid beetle (Caryedes brasiliensis) from northwestern Costa Rica.
Insect Biochem Mol Biol. 2013 Dec;43(12):1172-80.
|
| pEU-Gm23 | arginyl-tRNA synthetase (Other) | Synthetic Biology | SP6 |
Igloi
|
Wheat germ in vitro translation of soybean arginyl-tRNA synthetase
(unpublished)
|
| pBbE1k-2 | dihydropterine reductase, pterin-4a-carbinolamine dehydratase | | pTrc, pTrc |
Lee
| Engineering of L-tyrosine oxidation in Escherichia coli and microbial production of hydroxytyrosol.
Metab Eng. 2012 Nov;14(6):603-10. doi: 10.1016/j.ymben.2012.08.002. Epub 2012 Aug 29.
|
| pBAMD1-2 | | Synthetic Biology ; Mini-Tn5 vector | |
de Lorenzo
| New transposon tools tailored for metabolic engineering of gram-negative microbial cell factories.
Front Bioeng Biotechnol. 2014 Oct 28;2:46. doi: 10.3389/fbioe.2014.00046. eCollection 2014.
|
| pBAMD1-4 | | Synthetic Biology ; Mini-Tn5 vector | |
de Lorenzo
| New transposon tools tailored for metabolic engineering of gram-negative microbial cell factories.
Front Bioeng Biotechnol. 2014 Oct 28;2:46. doi: 10.3389/fbioe.2014.00046. eCollection 2014.
|
| pBAMD1-6 | | Synthetic Biology ; Mini-Tn5 vector | |
de Lorenzo
| New transposon tools tailored for metabolic engineering of gram-negative microbial cell factories.
Front Bioeng Biotechnol. 2014 Oct 28;2:46. doi: 10.3389/fbioe.2014.00046. eCollection 2014.
|
| p148 | flavaone 3B-hydroxylase (Other), dihydroflavonol 4-reductase (Other), leucoanthocyanidin reductase (Other) | Bacterial Expression, Synthetic Biology | T7, T7, T7 |
Koffas
| Improvement of catechin production in Escherichia coli through combinatorial metabolic engineering.
Metab Eng. 2014 Dec 17;28C:43-53. doi: 10.1016/j.ymben.2014.12.002.
|
| p158 | flavaone 3B-hydroxylase (Other), dihydroflavonol 4-reductase (Other), leucoanthocyanidin reductase (Other) | Bacterial Expression, Synthetic Biology | T7, T7, T7 |
Koffas
| Improvement of catechin production in Escherichia coli through combinatorial metabolic engineering.
Metab Eng. 2014 Dec 17;28C:43-53. doi: 10.1016/j.ymben.2014.12.002.
|
| p168 | flavaone 3B-hydroxylase (Other), dihydroflavonol 4-reductase (Other), leucoanthocyanidin reductase (Other) | Bacterial Expression, Synthetic Biology | T7, T7, T7 |
Koffas
| Improvement of catechin production in Escherichia coli through combinatorial metabolic engineering.
Metab Eng. 2014 Dec 17;28C:43-53. doi: 10.1016/j.ymben.2014.12.002.
|
| p168-759 | flavaone 3B-hydroxylase (Other), dihydroflavonol 4-reductase (Other), leucoanthocyanidin reductase (Other), Scaffold Protein encoding GBD:SH3:PDZ (1:1:4) (Synthetic) | Bacterial Expression, Synthetic Biology | T7, T7, T7, T7 |
Koffas
| Improvement of catechin production in Escherichia coli through combinatorial metabolic engineering.
Metab Eng. 2014 Dec 17;28C:43-53. doi: 10.1016/j.ymben.2014.12.002.
|
| p1585588 | flavaone 3B-hydroxylase (Other), dihydroflavonol 4-reductase (Other), leucoanthocyanidin reductase (Other), dihydroflavonol 4-reductase (Other), dihydroflavonol 4-reductase (Other), leucoanthocyanidin reductase (Other), leucoanthocyanidin reductase (Other) | Bacterial Expression, Synthetic Biology | T7, T7, T7, T7, T7, T7, T7 |
Koffas
| Improvement of catechin production in Escherichia coli through combinatorial metabolic engineering.
Metab Eng. 2014 Dec 17;28C:43-53. doi: 10.1016/j.ymben.2014.12.002.
|
| LAMP1-ABKAR | ABKAR biosensor | Mammalian Expression | CMV |
Inoue
| Compartmentalized AMPK Signaling Illuminated by Genetically Encoded Molecular Sensors and Actuators
Cell Reports
|
| pDCAF3-IS5 | ndmA (Other), ndmB (Other), ndmC (Other), ndmD (Other), gst9 (Other), chlR (Other) | Bacterial Expression, Synthetic Biology | BBa_J23100, BBa_J23100, BBa_J23100, BBa_J23100, BBa_J23100 |
Barrick
| Decaffeination and measurement of caffeine content by addicted Escherichia coli with a refactored N-demethylation operon from Pseudomonas putida CBB5.
ACS Synth Biol. 2013 Jun 21;2(6):301-7. doi: 10.1021/sb4000146. Epub 2013 Mar 22.
|
| HcKan_P | RFP (Other) | Synthetic Biology | |
Dai
| YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae.
Nucleic Acids Res. 2015 May 8. pii: gkv464.
|
| HcKan_O | RFP (Other) | Synthetic Biology | |
Dai
| YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae.
Nucleic Acids Res. 2015 May 8. pii: gkv464.
|
| HcKan_T | RFP (Other) | Synthetic Biology | |
Dai
| YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae.
Nucleic Acids Res. 2015 May 8. pii: gkv464.
|
| POT2-RFP | RFP (Other) | Synthetic Biology | |
Dai
| YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae.
Nucleic Acids Res. 2015 May 8. pii: gkv464.
|
| POT3-RFP | RFP (Other) | Synthetic Biology | |
Dai
| YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae.
Nucleic Acids Res. 2015 May 8. pii: gkv464.
|
| POT4-RFP | RFP (Other) | Synthetic Biology | |
Dai
| YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae.
Nucleic Acids Res. 2015 May 8. pii: gkv464.
|
| POT5-RFP | RFP (Other) | Synthetic Biology | |
Dai
| YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae.
Nucleic Acids Res. 2015 May 8. pii: gkv464.
|
| POT6-RFP | RFP (Other) | Synthetic Biology | |
Dai
| YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae.
Nucleic Acids Res. 2015 May 8. pii: gkv464.
|
| POT7-RFP | RFP (Other) | Synthetic Biology | |
Dai
| YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae.
Nucleic Acids Res. 2015 May 8. pii: gkv464.
|
| POT8-RFP | RFP (Other) | Synthetic Biology | |
Dai
| YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae.
Nucleic Acids Res. 2015 May 8. pii: gkv464.
|
| POT9-RFP | RFP (Other) | Synthetic Biology | |
Dai
| YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae.
Nucleic Acids Res. 2015 May 8. pii: gkv464.
|
| POT10-RFP | RFP (Other) | Synthetic Biology | |
Dai
| YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae.
Nucleic Acids Res. 2015 May 8. pii: gkv464.
|
| POT11-RFP | RFP (Other) | Synthetic Biology | |
Dai
| YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae.
Nucleic Acids Res. 2015 May 8. pii: gkv464.
|
| pRS414-URR | URR1-RFP-Leu2-URR2 (Synthetic) | Synthetic Biology | |
Dai
| YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae.
Nucleic Acids Res. 2015 May 8. pii: gkv464.
|
| pRS424-URR | URR1-RFP-Leu2-URR2 (Synthetic) | Synthetic Biology | |
Dai
| YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae.
Nucleic Acids Res. 2015 May 8. pii: gkv464.
|
| pMV-URR1 | URR1 (Synthetic) | Synthetic Biology | |
Dai
| YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae.
Nucleic Acids Res. 2015 May 8. pii: gkv464.
|
| pMV-URR2 | URR2 (Synthetic) | Synthetic Biology | |
Dai
| YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae.
Nucleic Acids Res. 2015 May 8. pii: gkv464.
|
| pMV-HIS3 | HIS3 (Saccharomyces cerevisiae) | Synthetic Biology | |
Dai
| YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae.
Nucleic Acids Res. 2015 May 8. pii: gkv464.
|
| pMV-LEU2 | LEU2 (Saccharomyces cerevisiae) | Synthetic Biology | |
Dai
| YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae.
Nucleic Acids Res. 2015 May 8. pii: gkv464.
|
| pMV-TRP1 | TRP1 (Saccharomyces cerevisiae) | Synthetic Biology | |
Dai
| YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae.
Nucleic Acids Res. 2015 May 8. pii: gkv464.
|
| HCKan-P-TDH3 | TDH3 promoter (Saccharomyces cerevisiae) | Synthetic Biology | |
Dai
| YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae.
Nucleic Acids Res. 2015 May 8. pii: gkv464.
|
| HCKan-P-TEF2 | TEF2 promoter (Saccharomyces cerevisiae) | Synthetic Biology | |
Dai
| YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae.
Nucleic Acids Res. 2015 May 8. pii: gkv464.
|
| HCKan-P-CYC1 | CYC1 promoter (Saccharomyces cerevisiae) | Synthetic Biology | |
Dai
| YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae.
Nucleic Acids Res. 2015 May 8. pii: gkv464.
|
| HCKan-T-ADH1 | ADH1 terminator (Saccharomyces cerevisiae) | Synthetic Biology | |
Dai
| YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae.
Nucleic Acids Res. 2015 May 8. pii: gkv464.
|
| HCKan-T-TEF2 | TEF2 terminator (Saccharomyces cerevisiae) | Synthetic Biology | |
Dai
| YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae.
Nucleic Acids Res. 2015 May 8. pii: gkv464.
|
| PMV-ycrtE | ycrtE (Synthetic) | Synthetic Biology | |
Dai
| YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae.
Nucleic Acids Res. 2015 May 8. pii: gkv464.
|
| PMV-ycrtI | ycrtI (Synthetic) | Synthetic Biology | |
Dai
| YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae.
Nucleic Acids Res. 2015 May 8. pii: gkv464.
|
| PMV-ycrtYB | ycrtYB (Synthetic) | Synthetic Biology | |
Dai
| YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae.
Nucleic Acids Res. 2015 May 8. pii: gkv464.
|
| HCKan-O-ycrtE | ycrtE (Synthetic) | Synthetic Biology | |
Dai
| YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae.
Nucleic Acids Res. 2015 May 8. pii: gkv464.
|
| HCKan-O-ycrtI | ycrtI (Synthetic) | Synthetic Biology | |
Dai
| YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae.
Nucleic Acids Res. 2015 May 8. pii: gkv464.
|
| HCKan-O-ycrtYB | ycrtYB (Synthetic) | Synthetic Biology | |
Dai
| YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae.
Nucleic Acids Res. 2015 May 8. pii: gkv464.
|
| pKMV-VioA | vioA (Synthetic) | Synthetic Biology | |
Dai
| YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae.
Nucleic Acids Res. 2015 May 8. pii: gkv464.
|
| pKMV-VioB | vioB (Synthetic) | Synthetic Biology | |
Dai
| YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae.
Nucleic Acids Res. 2015 May 8. pii: gkv464.
|
| pKMV-VioC | vioC (Synthetic) | Synthetic Biology | |
Dai
| YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae.
Nucleic Acids Res. 2015 May 8. pii: gkv464.
|
| pKMV-VioD | vioD (Synthetic) | Synthetic Biology | |
Dai
| YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae.
Nucleic Acids Res. 2015 May 8. pii: gkv464.
|
| pKMV-VioE | vioE (Synthetic) | Synthetic Biology | |
Dai
| YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae.
Nucleic Acids Res. 2015 May 8. pii: gkv464.
|
| pKS042 | clpA | Bacterial Expression, Synthetic Biology | trc |
Tyo
| N-Terminal-Based Targeted, Inducible Protein Degradation in Escherichia coli.
PLoS One. 2016 Feb 22;11(2):e0149746. doi: 10.1371/journal.pone.0149746. eCollection 2016.
|
| pKS043 | clpS | Bacterial Expression, Synthetic Biology | trc |
Tyo
| N-Terminal-Based Targeted, Inducible Protein Degradation in Escherichia coli.
PLoS One. 2016 Feb 22;11(2):e0149746. doi: 10.1371/journal.pone.0149746. eCollection 2016.
|
| pKS044 | clpP | Bacterial Expression, Synthetic Biology | trc |
Tyo
| N-Terminal-Based Targeted, Inducible Protein Degradation in Escherichia coli.
PLoS One. 2016 Feb 22;11(2):e0149746. doi: 10.1371/journal.pone.0149746. eCollection 2016.
|
| PAS501 (TJF040) | FadD with Q338R mutation (Other) | Bacterial Expression | T7/lacO |
Silver
| Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids.
PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
|
| PAS500 (TJF032) | FadD (Other) | Bacterial Expression | T7 lacO |
Silver
| Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids.
PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
|
| PAS502 (TJF065) | FadD (Other) | Bacterial Expression | T7 lacO |
Silver
| Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids.
PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
|
| PAS503 (TJF066) | FadD with V4F, W5L mutations (Other) | Bacterial Expression | T7 lacO |
Silver
| Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids.
PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
|
| PAS504 (TJF067) | FadD with F447S mutation (Other) | Bacterial Expression | T7 lacO |
Silver
| Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids.
PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
|
| PAS505 (TJF068) | FadD with V451A mutation (Other) | Bacterial Expression | T7 lacO |
Silver
| Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids.
PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
|
| PAS506 (TJF069) | FadD D372G (Other) | Bacterial Expression | T7 lacO |
Silver
| Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids.
PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
|
| PAS507 (TJF070) | FadD (Other) | Bacterial Expression | T7 lacO |
Silver
| Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids.
PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
|
| PAS508 (TJF071) | FadD (Other) | Bacterial Expression | T7 lacO |
Silver
| Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids.
PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
|
| PAS509 (TJF032 L448S) | FadD (Other) | Bacterial Expression | T7 lacO |
Silver
| Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids.
PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
|
| PAS510 (TJF032 G446S) | FadD (Other) | Bacterial Expression | T7 lacO |
Silver
| Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids.
PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
|
| PAS511 (TJF032 Y375R) | FadD (Other) | Bacterial Expression | T7 lacO |
Silver
| Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids.
PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
|
| PAS512 (TJF032 S377R) | FadD (Other) | Bacterial Expression | T7 lacO |
Silver
| Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids.
PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
|
| PAS513 (TJF080) | FadD (Other) | Bacterial Expression | T7 lacO |
Silver
| Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids.
PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
|
| PAS514 (TJF081) | FadD (Other) | Bacterial Expression | T7 lacO |
Silver
| Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids.
PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
|
| PAS515 (TJF082) | FadD (Other) | Bacterial Expression | T7 lacO |
Silver
| Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids.
PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
|
| PAS516 (TJF083) | FadD (Other) | Bacterial Expression | T7 lacO |
Silver
| Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids.
PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
|
| PAS517 (TJF084) | FadD (Other) | Bacterial Expression | T7 lacO |
Silver
| Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids.
PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
|
| PAS518 (TJF085) | FadD (Other) | Bacterial Expression | T7 lacO |
Silver
| Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids.
PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
|
| PAS519 (TJF086) | FadD (Other) | Bacterial Expression | T7 lacO |
Silver
| Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids.
PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
|
| PAS520 (TJF087) | FadD (Other) | Bacterial Expression | T7 lacO |
Silver
| Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids.
PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
|
| PAS521 (TJF088) | FadD (Other) | Bacterial Expression | T7 lacO |
Silver
| Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids.
PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
|
| PAS522 (TJF089) | FadD (Other) | Bacterial Expression | T7 lacO |
Silver
| Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids.
PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
|
| PAS523 (TJF090) | FadD (Other) | Bacterial Expression | T7 lacO |
Silver
| Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids.
PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
|
| PAS524 (TJF091) | FadD (Other) | Bacterial Expression | T7 lacO |
Silver
| Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids.
PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
|
| PAS525 (TJF092) | FadD (Other) | Bacterial Expression | T7 lacO |
Silver
| Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids.
PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
|
| PAS526 (TJF093) | FadD (Other) | Bacterial Expression | T7 lacO |
Silver
| Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids.
PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
|
| PAS527 (TJF094) | FadD (Other) | Bacterial Expression | T7 lacO |
Silver
| Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids.
PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
|
| PAS528 (TJF059) | FadD (Other) | Bacterial Expression | T7 lacO |
Silver
| Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids.
PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
|
| PAS529 (TJF072) | FadD (Other) | Bacterial Expression | T7 lacO |
Silver
| Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids.
PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
|
| PAS530 (TJF073) | FadD (Other) | Bacterial Expression | T7 lacO |
Silver
| Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids.
PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
|
| PAS531 (TJF074) | FadD (Other) | Bacterial Expression | T7 lacO |
Silver
| Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids.
PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
|
| PAS532 (TJF075) | FadD (Other) | Bacterial Expression | T7 lacO |
Silver
| Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids.
PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
|
| PAS533 (TJF076) | FadD (Other) | Bacterial Expression | T7 lacO |
Silver
| Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids.
PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
|
| PAS534 (TJF077) | FadD (Other) | Bacterial Expression | T7 lacO |
Silver
| Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids.
PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
|
| PAS535 (TJF078) | FadD (Other) | Bacterial Expression | T7 lacO |
Silver
| Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids.
PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
|
| PAS536 (TJF079) | FadD (Other) | Bacterial Expression | T7 lacO |
Silver
| Enhancement of E. coli acyl-CoA synthetase FadD activity on medium chain fatty acids.
PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 2015.
|
| pETM6-C4-mCherry | mCherry (Synthetic) | Bacterial Expression, Synthetic Biology | Mutant T7 Promoter - C4 |
Koffas
| ePathOptimize: A Combinatorial Approach for Transcriptional Balancing of Metabolic Pathways.
Sci Rep. 2015 Jun 11;5:11301. doi: 10.1038/srep11301.
|
| pETM6-H10-mCherry | mCherry (Synthetic) | Bacterial Expression, Synthetic Biology | Mutant T7 Promoter - H10 |
Koffas
| ePathOptimize: A Combinatorial Approach for Transcriptional Balancing of Metabolic Pathways.
Sci Rep. 2015 Jun 11;5:11301. doi: 10.1038/srep11301.
|
| pETM6-H9-mCherry | mCherry (Synthetic) | Bacterial Expression, Synthetic Biology | Mutant T7 Promoter - H9 |
Koffas
| ePathOptimize: A Combinatorial Approach for Transcriptional Balancing of Metabolic Pathways.
Sci Rep. 2015 Jun 11;5:11301. doi: 10.1038/srep11301.
|
| pETM6-G6-mCherry | mCherry (Synthetic) | Bacterial Expression, Synthetic Biology | Mutant T7 Promoter - G6 |
Koffas
| ePathOptimize: A Combinatorial Approach for Transcriptional Balancing of Metabolic Pathways.
Sci Rep. 2015 Jun 11;5:11301. doi: 10.1038/srep11301.
|
| pETM6-mCherry | mCherry (Synthetic) | Bacterial Expression, Synthetic Biology | Consensus T7 |
Koffas
| ePathOptimize: A Combinatorial Approach for Transcriptional Balancing of Metabolic Pathways.
Sci Rep. 2015 Jun 11;5:11301. doi: 10.1038/srep11301.
|
| pETM6-vioABECD | VioA (Other), VioB (Other), VioE (Other), VioC (Other), VioD (Other) | Bacterial Expression, Synthetic Biology | Consensus T7, Consensus T7, Consensus T7, Consensus T7, Consensus T7 |
Koffas
| ePathOptimize: A Combinatorial Approach for Transcriptional Balancing of Metabolic Pathways.
Sci Rep. 2015 Jun 11;5:11301. doi: 10.1038/srep11301.
|
| pETM6-G6-vioABECD | VioA (Other), VioB (Other), VioE (Other), VioC (Other), VioD (Other) | Bacterial Expression, Synthetic Biology | Mutant T7 Promoter - G6, Mutant T7 Promoter - G6, Mutant T7 Promoter - G6, Mutant T7 Promoter - G6, Mutant T7 Promoter - G6 |
Koffas
| ePathOptimize: A Combinatorial Approach for Transcriptional Balancing of Metabolic Pathways.
Sci Rep. 2015 Jun 11;5:11301. doi: 10.1038/srep11301.
|
| pETM6-E12-vioABECD | VioA (Other), VioB (Other), VioE (Other), VioC (Other), VioD (Other) | Bacterial Expression, Synthetic Biology | Mutant T7 Promoter - H10, Mutant T7 Promoter - G6, Mutant T7 Promoter - G6, Mutant T7 Promoter - H10, Mutant T7 Promoter - G6 |
Koffas
| ePathOptimize: A Combinatorial Approach for Transcriptional Balancing of Metabolic Pathways.
Sci Rep. 2015 Jun 11;5:11301. doi: 10.1038/srep11301.
|
| pETM6-M.accABCD | accA (Other), accB (Other), accC (Other), accD (Other) | Bacterial Expression, Synthetic Biology | T7, T7, T7, T7 |
Koffas
| Modular optimization of multi-gene pathways for fatty acids production in E. coli.
Nat Commun. 2013;4:1409. doi: 10.1038/ncomms2425.
|
| pETM6-P.accABCD | accA (Other), accB (Other), accC (Other), accD (Other) | Bacterial Expression, Synthetic Biology | T7, T7, T7, T7 |
Koffas
| Modular optimization of multi-gene pathways for fatty acids production in E. coli.
Nat Commun. 2013;4:1409. doi: 10.1038/ncomms2425.
|
| pWKS1583 | amyEopt (Synthetic) | Bacterial Expression, Synthetic Biology | Ptet |
Smits
| The Signal Sequence of the Abundant Extracellular Metalloprotease PPEP-1 Can Be Used to Secrete Synthetic Reporter Proteins in Clostridium difficile.
ACS Synth Biol. 2016 Jun 23.
|
| pETM6-HpaBC | HpaB (Other), HpaC (Other) | Bacterial Expression, Synthetic Biology | T7, T7 |
Koffas
| "Optimization of naringenin and p-coumaric acid hydroxylation using the native E. coli hydroxylase complex, HpaBC".
Biotechnol Prog. 2015 Oct 21. doi: 10.1002/btpr.2185.
|
| pBbE1k-3 | dihydropterine reductase (Homo sapiens), pterin-4a-carbinolamine dehydratase (Homo sapiens) | | pTrc, pTrc |
Lee
| Engineering of L-tyrosine oxidation in Escherichia coli and microbial production of hydroxytyrosol.
Metab Eng. 2012 Nov;14(6):603-10. doi: 10.1016/j.ymben.2012.08.002. Epub 2012 Aug 29.
|
| pBbE1k-DDC | dopa decarboxylase (Other) | | pTrc |
Lee
| Engineering of L-tyrosine oxidation in Escherichia coli and microbial production of hydroxytyrosol.
Metab Eng. 2012 Nov;14(6):603-10. doi: 10.1016/j.ymben.2012.08.002. Epub 2012 Aug 29.
|
| pJK108 | delta-12 fatty acid acetylenase (Other) | Bacterial Expression | T7 |
Kappock
|
Additional fatty acid desaturase and acetylenase constructs
(unpublished)
|
| PsbA2-HMGS-HMGR-ATOB-NPTI | HMGS-HMGR-ATOB-NPTI (Other) | | psbA2 |
Melis
|
Expression of the MVA pathway in cyanobacteria
(unpublished)
|
| pETM6-G6-vioABE | PG6-vioABE (Other) | Bacterial Expression, CRISPR, Synthetic Biology | PG6 (orthogonal T7-lac variant) |
Koffas
| Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli.
Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
|
| pETM6-G6-vioABE-4A6-vioC | PG6-vioABE-P4A6-vioC (Other) | Bacterial Expression, CRISPR, Synthetic Biology | PG6 and P4A6 (orthogonal T7-lac variants) |
Koffas
| Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli.
Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
|
| pETM6-G6-vioABE-4A6-vioC-3A2-vioD | PG6-vioABE-P4A6-vioC-P3A2-vioD (Other) | Bacterial Expression, CRISPR, Synthetic Biology | PG6, P4A6, and P3A2 (orthogonal T7-lac variants) |
Koffas
| Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli.
Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
|
| pJKR-H-benM | muconate biosensing apparatus | | |
Church
| Genetically encoded sensors enable real-time observation of metabolite production.
Proc Natl Acad Sci U S A. 2016 Feb 8. pii: 201600375.
|
| pZ8-P_dCas9 | dcas9 | Bacterial Expression, CRISPR, Synthetic Biology | Propionate inducible promoter (prp) |
Lu
| Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi).
ACS Synth Biol. 2016 Feb 16.
|
| pZ8-Ptac | | Bacterial Expression, Synthetic Biology | ptac |
Lu
| Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi).
ACS Synth Biol. 2016 Feb 16.
|
| pAL-pgi_T | sgRNA pgi (T) | Bacterial Expression, CRISPR, Synthetic Biology | ptac |
Lu
| Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi).
ACS Synth Biol. 2016 Feb 16.
|
| pAL-pgi_NT | sgRNA pgi (NT) (Synthetic) | Bacterial Expression, CRISPR, Synthetic Biology | ptac |
Lu
| Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi).
ACS Synth Biol. 2016 Feb 16.
|
| pAL-pck_T | sgRNA pck (T) (Synthetic) | Bacterial Expression, CRISPR, Synthetic Biology | ptac |
Lu
| Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi).
ACS Synth Biol. 2016 Feb 16.
|
| pAL-pck_NT | sgRNA pck (NT) (Synthetic) | Bacterial Expression, CRISPR, Synthetic Biology | ptac |
Lu
| Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi).
ACS Synth Biol. 2016 Feb 16.
|
| pAL-pyk_T | sgRNA pyk (T) (Synthetic) | Bacterial Expression, CRISPR, Synthetic Biology | ptac |
Lu
| Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi).
ACS Synth Biol. 2016 Feb 16.
|
| pAL-pyk_NT | sgRNA pyk (NT) (Synthetic) | Bacterial Expression, CRISPR | ptac |
Lu
| Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi).
ACS Synth Biol. 2016 Feb 16.
|
| pAL-rfp_T | sgRNA rfp (T) (Synthetic) | Bacterial Expression, Synthetic Biology | ptac |
Lu
| Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi).
ACS Synth Biol. 2016 Feb 16.
|
| PP259 | GFP | Yeast Expression | |
Lu
| Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care.
Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
|
| PP257 | GFP | Yeast Expression | |
Lu
| Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care.
Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
|
| PP245 | GFP | Yeast Expression | |
Lu
| Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care.
Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
|
| PP277 | GFP | Yeast Expression | |
Lu
| Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care.
Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
|
| PP190 | GFP | Yeast Expression | |
Lu
| Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care.
Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
|
| PP273 | GFP | Yeast Expression | |
Lu
| Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care.
Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
|
| PP160 | GFP | Yeast Expression | |
Lu
| Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care.
Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
|
| PP155 | GFP | Yeast Expression | |
Lu
| Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care.
Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
|
| PP163 | GFP | Yeast Expression | |
Lu
| Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care.
Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
|
Synthetic Biology: Metabolism