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Musunuru/Cowan Lab TALEN Kit
(Kit # 1000000034 )

Depositing Labs:   Kiran Musunuru, Chad Cowan

TALENs are fusions of transcription activator-like (TAL) effectors of plant pathogenic Xanthomonas spp. to the FokI nuclease. TALENs bind and cleave DNA in pairs, and their binding specificity is determined by customizable arrays of polymorphic amino acid repeats in the TAL effectors. This collection and accompanying documentation allow one to efficiently assemble TALEN constructs with custom repeat arrays, each containing 15 repeats.

This kit consists of 9 plates, and will be shipped as bacterial glycerol stocks, 96-well format, on dry ice. Samples should be frozen at -80 degrees Celsius immediately upon arrival. Individual plasmids can be ordered at $65 per plasmid, and will be shipped as bacterial stabs.

Add to Cart
$1400 USD + shipping

Available to academics and nonprofits only.

Original Publication

A TALEN Genome-Editing System for Generating Human Stem Cell-Based Disease Models. Ding Q, Lee Y, Schaefer E, Peters D, Veres A, Kim K, Kuperwasser N, Motola D, Meissner T, Hendriks W, Trevisan M, Gupta R, Moisan A, Banks E, Friesen M, Schinzel R, Xia F, Tang A, Xia Y, Figueroa E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin L, Peng L, Chung R, Musunuru K, Cowan C. Cell Stem Cell . 2013 Feb 7;12(2):238-51. doi: 10.1016/j.stem.2012.11.011. Epub 2012 Dec 13. PubMed .

Description

Transcription activator-like effector nucleases (TALENs) are a new class of engineered nucleases that, due to their modular domain structure, have proven more straightforward to design and construct for performing genome editing than other types of nucleases. TALENs are typically designed as a pair that binds to genomic sequences flanking a target site and generates a double-strand break, which is repaired by the cell using either homology-directed repair (HDR) or the error-prone process of nonhomologous end-joining (NHEJ).

talen-musunuru-cowan-figure-1.png

The DNA-binding domain of a TALEN comprises an array of 34-amino-acid monomers that are ‘‘coded’’ to recognize and bind specific DNA base pairs (bp) in a 1:1 fashion. We have built upon previously described modular Golden Gate methodologies to allow assembly of multiple DNA fragments in an ordered fashion, such that a single ligation of preassembled tetramers and trimers generates TALENs that recognize any 15 bp recognition site in the genome.

This assembly method requires only 1-2 days for completion and is not prone to errors that complicate methods that rely on PCR amplification of monomers. Furthermore, we have developed a set of optimized vectors and methods for the delivery of TALENs into mammalian cells and, in particular, human pluripotent stem cells and other difficult-to-transfect cell types.

In brief, we transfect or electroporate TALEN pairs into cells and then subject them to fluorescence-activated cell sorting (FACS) 48 hr posttransfection based on fluorescent-marker expression. We replate the sorted cells at low density and allow them to recover and grow for 1 week, resulting in the formation of distinct single colonies. Colonies are expanded, genomic DNA purified, and mutations analyzed by PCR, agarose-gel screening, and Sanger sequencing. The entire process from start to finish can be completed in less than one month.

TALEN Illustration

To assemble a TALEN that recognizes the sequence N1-N2-N3-N4-N5-N6-N7-N8-N9-N10-N11-N12-N13-N14-N15, use the plasmids called I-N1-N2-N3-N4, II-N5-N6-N7-N8, III-N9-N10-N11-N12, and IV-N13-N14-N15. (It is optimal, but not essential, for the nucleotide preceding N1 to be T, as TALENs have an intrinsic preference for a T in the "N0" position.)

For example, to recognize the sequence 5'-GATGATCTCAGAGGC-3', use the plasmids called I-GATG, II-ATCT, III-CAGA, and IV-GGC. These plasmids can be ordered either individually or as part of the full 834-plasmid Kit.

The inserts from these plasmids are then assembled into either pTAL_GFP or pTAL_RFP via a single ligation reaction. To create a working TALEN pair, one of the TALENs should be assembled in pTAL_GFP and the other in pTAL_RFP, as the two pTAL plasmids encode distinct FokI nuclease domains that form an obligate heterodimer.

The full sequences for the pTAL_GFP and pTAL_RFP plasmids in GenBank format, as well as a spreadsheet with the insert sequences of the I/II/III/IV vectors, is available as a ZIP file (see "Protocols & Resources" tab).

Kit Documentation

Icon Musunuru/Cowan Lab TALEN Kit Info (93.9 KB)

Protocols

A detailed protocol for the assembly of TALENs with this Kit and their use to perform genome editing of cells is available at StemBook . A PDF of the protocol can be downloaded from that page.

Links

Icon Musunuru/Cowan Lab TALEN Kit Sequences (51.7 KB)

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:
"The plasmid kit used for generation of TALENs was a gift from Kiran Musunuru and Chad Cowan (Addgene kit # 1000000034)"

For your Reference section:
A TALEN Genome-Editing System for Generating Human Stem Cell-Based Disease Models. Ding Q, Lee Y, Schaefer E, Peters D, Veres A, Kim K, Kuperwasser N, Motola D, Meissner T, Hendriks W, Trevisan M, Gupta R, Moisan A, Banks E, Friesen M, Schinzel R, Xia F, Tang A, Xia Y, Figueroa E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin L, Peng L, Chung R, Musunuru K, Cowan C. Cell Stem Cell . 2013 Feb 7;12(2):238-51. doi: 10.1016/j.stem.2012.11.011. Epub 2012 Dec 13. PubMed .

Musunuru-Cowan TALEN Kit - #1000000034

Resistance Color Key

Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.

Inventory

Searchable and sortable table of all plasmids in kit. The Well column links to its kit plate map on the right. The Plasmid column links to a plasmid's individual web page.

Kit Plate Map

96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.

Resistance Color Key

Ampicillin and Kanamycin
Kanamycin
Ampicillin

Inventory

Well Plasmid Resistance
Plate 1 / A / 1 I-AAAA
Ampicillin and Kanamycin
Plate 1 / A / 2 I-AAAC
Ampicillin and Kanamycin
Plate 1 / A / 3 I-AAAG
Ampicillin and Kanamycin
Plate 1 / A / 4 I-AAAT
Ampicillin and Kanamycin
Plate 1 / A / 5 I-AACA
Ampicillin and Kanamycin
Plate 1 / A / 6 I-AACC
Ampicillin and Kanamycin
Plate 1 / A / 7 I-AACG
Ampicillin and Kanamycin
Plate 1 / A / 8 I-AACT
Ampicillin and Kanamycin
Plate 1 / A / 9 I-AAGA
Ampicillin and Kanamycin
Plate 1 / A / 10 I-AAGC
Ampicillin and Kanamycin
Plate 1 / A / 11 I-AAGG
Ampicillin and Kanamycin
Plate 1 / A / 12 I-AAGT
Ampicillin and Kanamycin
Plate 1 / B / 1 I-AATA
Ampicillin and Kanamycin
Plate 1 / B / 2 I-AATC
Ampicillin and Kanamycin
Plate 1 / B / 3 I-AATG
Ampicillin and Kanamycin
Plate 1 / B / 4 I-AATT
Ampicillin and Kanamycin
Plate 1 / B / 5 I-ACAA
Ampicillin and Kanamycin
Plate 1 / B / 6 I-ACAC
Ampicillin and Kanamycin
Plate 1 / B / 7 I-ACAG
Ampicillin and Kanamycin
Plate 1 / B / 8 I-ACAT
Ampicillin and Kanamycin
Plate 1 / B / 9 I-ACCA
Ampicillin and Kanamycin
Plate 1 / B / 10 I-ACCC
Ampicillin and Kanamycin
Plate 1 / B / 11 I-ACCG
Ampicillin and Kanamycin
Plate 1 / B / 12 I-ACCT
Ampicillin and Kanamycin
Plate 1 / C / 1 I-ACGA
Ampicillin and Kanamycin
Plate 1 / C / 2 I-ACGC
Ampicillin and Kanamycin
Plate 1 / C / 3 I-ACGG
Ampicillin and Kanamycin
Plate 1 / C / 4 I-ACGT
Ampicillin and Kanamycin
Plate 1 / C / 5 I-ACTA
Ampicillin and Kanamycin
Plate 1 / C / 6 I-ACTC
Ampicillin and Kanamycin
Plate 1 / C / 7 I-ACTG
Ampicillin and Kanamycin
Plate 1 / C / 8 I-ACTT
Ampicillin and Kanamycin
Plate 1 / C / 9 I-AGAA
Ampicillin and Kanamycin
Plate 1 / C / 10 I-AGAC
Ampicillin and Kanamycin
Plate 1 / C / 11 I-AGAG
Ampicillin and Kanamycin
Plate 1 / C / 12 I-AGAT
Ampicillin and Kanamycin
Plate 1 / D / 1 I-AGCA
Ampicillin and Kanamycin
Plate 1 / D / 2 I-AGCC
Ampicillin and Kanamycin
Plate 1 / D / 3 I-AGCG
Ampicillin and Kanamycin
Plate 1 / D / 4 I-AGCT
Ampicillin and Kanamycin
Plate 1 / D / 5 I-AGGA
Ampicillin and Kanamycin
Plate 1 / D / 6 I-AGGC
Ampicillin and Kanamycin
Plate 1 / D / 7 I-AGGG
Ampicillin and Kanamycin
Plate 1 / D / 8 I-AGGT
Ampicillin and Kanamycin
Plate 1 / D / 9 I-AGTA
Ampicillin and Kanamycin
Plate 1 / D / 10 I-AGTC
Ampicillin and Kanamycin
Plate 1 / D / 11 I-AGTG
Ampicillin and Kanamycin
Plate 1 / D / 12 I-AGTT
Ampicillin and Kanamycin
Plate 1 / E / 1 I-ATAA
Ampicillin and Kanamycin
Plate 1 / E / 2 I-ATAC
Ampicillin and Kanamycin
Plate 1 / E / 3 I-ATAG
Ampicillin and Kanamycin
Plate 1 / E / 4 I-ATAT
Ampicillin and Kanamycin
Plate 1 / E / 5 I-ATCA
Ampicillin and Kanamycin
Plate 1 / E / 6 I-ATCC
Ampicillin and Kanamycin
Plate 1 / E / 7 I-ATCG
Ampicillin and Kanamycin
Plate 1 / E / 8 I-ATCT
Ampicillin and Kanamycin
Plate 1 / E / 9 I-ATGA
Ampicillin and Kanamycin
Plate 1 / E / 10 I-ATGC
Ampicillin and Kanamycin
Plate 1 / E / 11 I-ATGG
Ampicillin and Kanamycin
Plate 1 / E / 12 I-ATGT
Ampicillin and Kanamycin
Plate 1 / F / 1 I-ATTA
Ampicillin and Kanamycin
Plate 1 / F / 2 I-ATTC
Ampicillin and Kanamycin
Plate 1 / F / 3 I-ATTG
Ampicillin and Kanamycin
Plate 1 / F / 4 I-ATTT
Ampicillin and Kanamycin
Plate 1 / F / 5 I-CAAA
Ampicillin and Kanamycin
Plate 1 / F / 6 I-CAAC
Ampicillin and Kanamycin
Plate 1 / F / 7 I-CAAG
Ampicillin and Kanamycin
Plate 1 / F / 8 I-CAAT
Ampicillin and Kanamycin
Plate 1 / F / 9 I-CACA
Ampicillin and Kanamycin
Plate 1 / F / 10 I-CACC
Ampicillin and Kanamycin
Plate 1 / F / 11 I-CACG
Ampicillin and Kanamycin
Plate 1 / F / 12 I-CACT
Ampicillin and Kanamycin
Plate 1 / G / 1 I-CAGA
Ampicillin and Kanamycin
Plate 1 / G / 2 I-CAGC
Ampicillin and Kanamycin
Plate 1 / G / 3 I-CAGG
Ampicillin and Kanamycin
Plate 1 / G / 4 I-CAGT
Ampicillin and Kanamycin
Plate 1 / G / 5 I-CATA
Ampicillin and Kanamycin
Plate 1 / G / 6 I-CATC
Ampicillin and Kanamycin
Plate 1 / G / 7 I-CATG
Ampicillin and Kanamycin
Plate 1 / G / 8 I-CATT
Ampicillin and Kanamycin
Plate 1 / G / 9 I-CCAA
Ampicillin and Kanamycin
Plate 1 / G / 10 I-CCAC
Ampicillin and Kanamycin
Plate 1 / G / 11 I-CCAG
Ampicillin and Kanamycin
Plate 1 / G / 12 I-CCAT
Ampicillin and Kanamycin
Plate 1 / H / 1 I-CCCA
Ampicillin and Kanamycin
Plate 1 / H / 2 I-CCCC
Ampicillin and Kanamycin
Plate 1 / H / 3 I-CCCG
Ampicillin and Kanamycin
Plate 1 / H / 4 I-CCCT
Ampicillin and Kanamycin
Plate 1 / H / 5 I-CCGA
Ampicillin and Kanamycin
Plate 1 / H / 6 I-CCGC
Ampicillin and Kanamycin
Plate 1 / H / 7 I-CCGG
Ampicillin and Kanamycin
Plate 1 / H / 8 I-CCGT
Ampicillin and Kanamycin
Plate 1 / H / 9 I-CCTA
Ampicillin and Kanamycin
Plate 1 / H / 10 I-CCTC
Ampicillin and Kanamycin
Plate 1 / H / 11 I-CCTG
Ampicillin and Kanamycin
Plate 1 / H / 12 I-CCTT
Ampicillin and Kanamycin
Plate 2 / A / 1 I-CGAA
Ampicillin and Kanamycin
Plate 2 / A / 2 I-CGAC
Ampicillin and Kanamycin
Plate 2 / A / 3 I-CGAG
Ampicillin and Kanamycin
Plate 2 / A / 4 I-CGAT
Ampicillin and Kanamycin
Plate 2 / A / 5 I-CGCA
Ampicillin and Kanamycin
Plate 2 / A / 6 I-CGCC
Ampicillin and Kanamycin
Plate 2 / A / 7 I-CGCG
Ampicillin and Kanamycin
Plate 2 / A / 8 I-CGCT
Ampicillin and Kanamycin
Plate 2 / A / 9 I-CGGA
Ampicillin and Kanamycin
Plate 2 / A / 10 I-CGGC
Ampicillin and Kanamycin
Plate 2 / A / 11 I-CGGG
Ampicillin and Kanamycin
Plate 2 / A / 12 I-CGGT
Ampicillin and Kanamycin
Plate 2 / B / 1 I-CGTA
Ampicillin and Kanamycin
Plate 2 / B / 2 I-CGTC
Ampicillin and Kanamycin
Plate 2 / B / 3 I-CGTG
Ampicillin and Kanamycin
Plate 2 / B / 4 I-CGTT
Ampicillin and Kanamycin
Plate 2 / B / 5 I-CTAA
Ampicillin and Kanamycin
Plate 2 / B / 6 I-CTAC
Ampicillin and Kanamycin
Plate 2 / B / 7 I-CTAG
Ampicillin and Kanamycin
Plate 2 / B / 8 I-CTAT
Ampicillin and Kanamycin
Plate 2 / B / 9 I-CTCA
Ampicillin and Kanamycin
Plate 2 / B / 10 I-CTCC
Ampicillin and Kanamycin
Plate 2 / B / 11 I-CTCG
Ampicillin and Kanamycin
Plate 2 / B / 12 I-CTCT
Ampicillin and Kanamycin
Plate 2 / C / 1 I-CTGA
Ampicillin and Kanamycin
Plate 2 / C / 2 I-CTGC
Ampicillin and Kanamycin
Plate 2 / C / 3 I-CTGG
Ampicillin and Kanamycin
Plate 2 / C / 4 I-CTGT
Ampicillin and Kanamycin
Plate 2 / C / 5 I-CTTA
Ampicillin and Kanamycin
Plate 2 / C / 6 I-CTTC
Ampicillin and Kanamycin
Plate 2 / C / 7 I-CTTG
Ampicillin and Kanamycin
Plate 2 / C / 8 I-CTTT
Ampicillin and Kanamycin
Plate 2 / C / 9 I-GAAA
Ampicillin and Kanamycin
Plate 2 / C / 10 I-GAAC
Ampicillin and Kanamycin
Plate 2 / C / 11 I-GAAG
Ampicillin and Kanamycin
Plate 2 / C / 12 I-GAAT
Ampicillin and Kanamycin
Plate 2 / D / 1 I-GACA
Ampicillin and Kanamycin
Plate 2 / D / 2 I-GACC
Ampicillin and Kanamycin
Plate 2 / D / 3 I-GACG
Ampicillin and Kanamycin
Plate 2 / D / 4 I-GACT
Ampicillin and Kanamycin
Plate 2 / D / 5 I-GAGA
Ampicillin and Kanamycin
Plate 2 / D / 6 I-GAGC
Ampicillin and Kanamycin
Plate 2 / D / 7 I-GAGG
Ampicillin and Kanamycin
Plate 2 / D / 8 I-GAGT
Ampicillin and Kanamycin
Plate 2 / D / 9 I-GATA
Ampicillin and Kanamycin
Plate 2 / D / 10 I-GATC
Ampicillin and Kanamycin
Plate 2 / D / 11 I-GATG
Ampicillin and Kanamycin
Plate 2 / D / 12 I-GATT
Ampicillin and Kanamycin
Plate 2 / E / 1 I-GCAA
Ampicillin and Kanamycin
Plate 2 / E / 2 I-GCAC
Ampicillin and Kanamycin
Plate 2 / E / 3 I-GCAG
Ampicillin and Kanamycin
Plate 2 / E / 4 I-GCAT
Ampicillin and Kanamycin
Plate 2 / E / 5 I-GCCA
Ampicillin and Kanamycin
Plate 2 / E / 6 I-GCCC
Ampicillin and Kanamycin
Plate 2 / E / 7 I-GCCG
Ampicillin and Kanamycin
Plate 2 / E / 8 I-GCCT
Ampicillin and Kanamycin
Plate 2 / E / 9 I-GCGA
Ampicillin and Kanamycin
Plate 2 / E / 10 I-GCGC
Ampicillin and Kanamycin
Plate 2 / E / 11 I-GCGG
Ampicillin and Kanamycin
Plate 2 / E / 12 I-GCGT
Ampicillin and Kanamycin
Plate 2 / F / 1 I-GCTA
Ampicillin and Kanamycin
Plate 2 / F / 2 I-GCTC
Ampicillin and Kanamycin
Plate 2 / F / 3 I-GCTG
Ampicillin and Kanamycin
Plate 2 / F / 4 I-GCTT
Ampicillin and Kanamycin
Plate 2 / F / 5 I-GGAA
Ampicillin and Kanamycin
Plate 2 / F / 6 I-GGAC
Ampicillin and Kanamycin
Plate 2 / F / 7 I-GGAG
Ampicillin and Kanamycin
Plate 2 / F / 8 I-GGAT
Ampicillin and Kanamycin
Plate 2 / F / 9 I-GGCA
Ampicillin and Kanamycin
Plate 2 / F / 10 I-GGCC
Ampicillin and Kanamycin
Plate 2 / F / 11 I-GGCG
Ampicillin and Kanamycin
Plate 2 / F / 12 I-GGCT
Ampicillin and Kanamycin
Plate 2 / G / 1 I-GGGA
Ampicillin and Kanamycin
Plate 2 / G / 2 I-GGGC
Ampicillin and Kanamycin
Plate 2 / G / 3 I-GGGG
Ampicillin and Kanamycin
Plate 2 / G / 4 I-GGGT
Ampicillin and Kanamycin
Plate 2 / G / 5 I-GGTA
Ampicillin and Kanamycin
Plate 2 / G / 6 I-GGTC
Ampicillin and Kanamycin
Plate 2 / G / 7 I-GGTG
Ampicillin and Kanamycin
Plate 2 / G / 8 I-GGTT
Ampicillin and Kanamycin
Plate 2 / G / 9 I-GTAA
Ampicillin and Kanamycin
Plate 2 / G / 10 I-GTAC
Ampicillin and Kanamycin
Plate 2 / G / 11 I-GTAG
Ampicillin and Kanamycin
Plate 2 / G / 12 I-GTAT
Ampicillin and Kanamycin
Plate 2 / H / 1 I-GTCA
Ampicillin and Kanamycin
Plate 2 / H / 2 I-GTCC
Ampicillin and Kanamycin
Plate 2 / H / 3 I-GTCG
Ampicillin and Kanamycin
Plate 2 / H / 4 I-GTCT
Ampicillin and Kanamycin
Plate 2 / H / 5 I-GTGA
Ampicillin and Kanamycin
Plate 2 / H / 6 I-GTGC
Ampicillin and Kanamycin
Plate 2 / H / 7 I-GTGG
Ampicillin and Kanamycin
Plate 2 / H / 8 I-GTGT
Ampicillin and Kanamycin
Plate 2 / H / 9 I-GTTA
Ampicillin and Kanamycin
Plate 2 / H / 10 I-GTTC
Ampicillin and Kanamycin
Plate 2 / H / 11 I-GTTG
Ampicillin and Kanamycin
Plate 2 / H / 12 I-GTTT
Ampicillin and Kanamycin
Plate 3 / A / 1 I-TAAA
Ampicillin and Kanamycin
Plate 3 / A / 2 I-TAAC
Ampicillin and Kanamycin
Plate 3 / A / 3 I-TAAG
Ampicillin and Kanamycin
Plate 3 / A / 4 I-TAAT
Ampicillin and Kanamycin
Plate 3 / A / 5 I-TACA
Ampicillin and Kanamycin
Plate 3 / A / 6 I-TACC
Ampicillin and Kanamycin
Plate 3 / A / 7 I-TACG
Ampicillin and Kanamycin
Plate 3 / A / 8 I-TACT
Ampicillin and Kanamycin
Plate 3 / A / 9 I-TAGA
Ampicillin and Kanamycin
Plate 3 / A / 10 I-TAGC
Ampicillin and Kanamycin
Plate 3 / A / 11 I-TAGG
Ampicillin and Kanamycin
Plate 3 / A / 12 I-TAGT
Ampicillin and Kanamycin
Plate 3 / B / 1 I-TATA
Ampicillin and Kanamycin
Plate 3 / B / 2 I-TATC
Ampicillin and Kanamycin
Plate 3 / B / 3 I-TATG
Ampicillin and Kanamycin
Plate 3 / B / 4 I-TATT
Ampicillin and Kanamycin
Plate 3 / B / 5 I-TCAA
Ampicillin and Kanamycin
Plate 3 / B / 6 I-TCAC
Ampicillin and Kanamycin
Plate 3 / B / 7 I-TCAG
Ampicillin and Kanamycin
Plate 3 / B / 8 I-TCAT
Ampicillin and Kanamycin
Plate 3 / B / 9 I-TCCA
Ampicillin and Kanamycin
Plate 3 / B / 10 I-TCCC
Ampicillin and Kanamycin
Plate 3 / B / 11 I-TCCG
Ampicillin and Kanamycin
Plate 3 / B / 12 I-TCCT
Ampicillin and Kanamycin
Plate 3 / C / 1 I-TCGA
Ampicillin and Kanamycin
Plate 3 / C / 2 I-TCGC
Ampicillin and Kanamycin
Plate 3 / C / 3 I-TCGG
Ampicillin and Kanamycin
Plate 3 / C / 4 I-TCGT
Ampicillin and Kanamycin
Plate 3 / C / 5 I-TCTA
Ampicillin and Kanamycin
Plate 3 / C / 6 I-TCTC
Ampicillin and Kanamycin
Plate 3 / C / 7 I-TCTG
Ampicillin and Kanamycin
Plate 3 / C / 8 I-TCTT
Ampicillin and Kanamycin
Plate 3 / C / 9 I-TGAA
Ampicillin and Kanamycin
Plate 3 / C / 10 I-TGAC
Ampicillin and Kanamycin
Plate 3 / C / 11 I-TGAG
Ampicillin and Kanamycin
Plate 3 / C / 12 I-TGAT
Ampicillin and Kanamycin
Plate 3 / D / 1 I-TGCA
Ampicillin and Kanamycin
Plate 3 / D / 2 I-TGCC
Ampicillin and Kanamycin
Plate 3 / D / 3 I-TGCG
Ampicillin and Kanamycin
Plate 3 / D / 4 I-TGCT
Ampicillin and Kanamycin
Plate 3 / D / 5 I-TGGA
Ampicillin and Kanamycin
Plate 3 / D / 6 I-TGGC
Ampicillin and Kanamycin
Plate 3 / D / 7 I-TGGG
Ampicillin and Kanamycin
Plate 3 / D / 8 I-TGGT
Ampicillin and Kanamycin
Plate 3 / D / 9 I-TGTA
Ampicillin and Kanamycin
Plate 3 / D / 10 I-TGTC
Ampicillin and Kanamycin
Plate 3 / D / 11 I-TGTG
Ampicillin and Kanamycin
Plate 3 / D / 12 I-TGTT
Ampicillin and Kanamycin
Plate 3 / E / 1 I-TTAA
Ampicillin and Kanamycin
Plate 3 / E / 2 I-TTAC
Ampicillin and Kanamycin
Plate 3 / E / 3 I-TTAG
Ampicillin and Kanamycin
Plate 3 / E / 4 I-TTAT
Ampicillin and Kanamycin
Plate 3 / E / 5 I-TTCA
Ampicillin and Kanamycin
Plate 3 / E / 6 I-TTCC
Ampicillin and Kanamycin
Plate 3 / E / 7 I-TTCG
Ampicillin and Kanamycin
Plate 3 / E / 8 I-TTCT
Ampicillin and Kanamycin
Plate 3 / E / 9 I-TTGA
Ampicillin and Kanamycin
Plate 3 / E / 10 I-TTGC
Ampicillin and Kanamycin
Plate 3 / E / 11 I-TTGG
Ampicillin and Kanamycin
Plate 3 / E / 12 I-TTGT
Ampicillin and Kanamycin
Plate 3 / F / 1 I-TTTA
Ampicillin and Kanamycin
Plate 3 / F / 2 I-TTTC
Ampicillin and Kanamycin
Plate 3 / F / 3 I-TTTG
Ampicillin and Kanamycin
Plate 3 / F / 4 I-TTTT
Ampicillin and Kanamycin
Plate 3 / F / 5 II-AAAA
Ampicillin and Kanamycin
Plate 3 / F / 6 II-AAAC
Ampicillin and Kanamycin
Plate 3 / F / 7 II-AAAG
Ampicillin and Kanamycin
Plate 3 / F / 8 II-AAAT
Ampicillin and Kanamycin
Plate 3 / F / 9 II-AACA
Ampicillin and Kanamycin
Plate 3 / F / 10 II-AACC
Ampicillin and Kanamycin
Plate 3 / F / 11 II-AACG
Ampicillin and Kanamycin
Plate 3 / F / 12 II-AACT
Ampicillin and Kanamycin
Plate 3 / G / 1 II-AAGA
Ampicillin and Kanamycin
Plate 3 / G / 2 II-AAGC
Ampicillin and Kanamycin
Plate 3 / G / 3 II-AAGG
Ampicillin and Kanamycin
Plate 3 / G / 4 II-AAGT
Ampicillin and Kanamycin
Plate 3 / G / 5 II-AATA
Ampicillin and Kanamycin
Plate 3 / G / 6 II-AATC
Ampicillin and Kanamycin
Plate 3 / G / 7 II-AATG
Ampicillin and Kanamycin
Plate 3 / G / 8 II-AATT
Ampicillin and Kanamycin
Plate 3 / G / 9 II-ACAA
Ampicillin and Kanamycin
Plate 3 / G / 10 II-ACAC
Ampicillin and Kanamycin
Plate 3 / G / 11 II-ACAG
Ampicillin and Kanamycin
Plate 3 / G / 12 II-ACAT
Ampicillin and Kanamycin
Plate 3 / H / 1 II-ACCA
Ampicillin and Kanamycin
Plate 3 / H / 2 II-ACCC
Ampicillin and Kanamycin
Plate 3 / H / 3 II-ACCG
Ampicillin and Kanamycin
Plate 3 / H / 4 II-ACCT
Ampicillin and Kanamycin
Plate 3 / H / 5 II-ACGA
Ampicillin and Kanamycin
Plate 3 / H / 6 II-ACGC
Ampicillin and Kanamycin
Plate 3 / H / 7 II-ACGG
Ampicillin and Kanamycin
Plate 3 / H / 8 II-ACGT
Ampicillin and Kanamycin
Plate 3 / H / 9 II-ACTA
Ampicillin and Kanamycin
Plate 3 / H / 10 II-ACTC
Ampicillin and Kanamycin
Plate 3 / H / 11 II-ACTG
Ampicillin and Kanamycin
Plate 3 / H / 12 II-ACTT
Ampicillin and Kanamycin
Plate 4 / A / 1 II-AGAA
Ampicillin and Kanamycin
Plate 4 / A / 2 II-AGAC
Ampicillin and Kanamycin
Plate 4 / A / 3 II-AGAG
Ampicillin and Kanamycin
Plate 4 / A / 4 II-AGAT
Ampicillin and Kanamycin
Plate 4 / A / 5 II-AGCA
Ampicillin and Kanamycin
Plate 4 / A / 6 II-AGCC
Ampicillin and Kanamycin
Plate 4 / A / 7 II-AGCG
Ampicillin and Kanamycin
Plate 4 / A / 8 II-AGCT
Ampicillin and Kanamycin
Plate 4 / A / 9 II-AGGA
Ampicillin and Kanamycin
Plate 4 / A / 10 II-AGGC
Ampicillin and Kanamycin
Plate 4 / A / 11 II-AGGG
Ampicillin and Kanamycin
Plate 4 / A / 12 II-AGGT
Ampicillin and Kanamycin
Plate 4 / B / 1 II-AGTA
Ampicillin and Kanamycin
Plate 4 / B / 2 II-AGTC
Ampicillin and Kanamycin
Plate 4 / B / 3 II-AGTG
Ampicillin and Kanamycin
Plate 4 / B / 4 II-AGTT
Ampicillin and Kanamycin
Plate 4 / B / 5 II-ATAA
Ampicillin and Kanamycin
Plate 4 / B / 6 II-ATAC
Ampicillin and Kanamycin
Plate 4 / B / 7 II-ATAG
Ampicillin and Kanamycin
Plate 4 / B / 8 II-ATAT
Ampicillin and Kanamycin
Plate 4 / B / 9 II-ATCA
Ampicillin and Kanamycin
Plate 4 / B / 10 II-ATCC
Ampicillin and Kanamycin
Plate 4 / B / 11 II-ATCG
Ampicillin and Kanamycin
Plate 4 / B / 12 II-ATCT
Ampicillin and Kanamycin
Plate 4 / C / 1 II-ATGA
Ampicillin and Kanamycin
Plate 4 / C / 2 II-ATGC
Ampicillin and Kanamycin
Plate 4 / C / 3 II-ATGG
Ampicillin and Kanamycin
Plate 4 / C / 4 II-ATGT
Ampicillin and Kanamycin
Plate 4 / C / 5 II-ATTA
Ampicillin and Kanamycin
Plate 4 / C / 6 II-ATTC
Ampicillin and Kanamycin
Plate 4 / C / 7 II-ATTG
Ampicillin and Kanamycin
Plate 4 / C / 8 II-ATTT
Ampicillin and Kanamycin
Plate 4 / C / 9 II-CAAA
Ampicillin and Kanamycin
Plate 4 / C / 10 II-CAAC
Ampicillin and Kanamycin
Plate 4 / C / 11 II-CAAG
Ampicillin and Kanamycin
Plate 4 / C / 12 II-CAAT
Ampicillin and Kanamycin
Plate 4 / D / 1 II-CACA
Ampicillin and Kanamycin
Plate 4 / D / 2 II-CACC
Ampicillin and Kanamycin
Plate 4 / D / 3 II-CACG
Ampicillin and Kanamycin
Plate 4 / D / 4 II-CACT
Ampicillin and Kanamycin
Plate 4 / D / 5 II-CAGA
Ampicillin and Kanamycin
Plate 4 / D / 6 II-CAGC
Ampicillin and Kanamycin
Plate 4 / D / 7 II-CAGG
Ampicillin and Kanamycin
Plate 4 / D / 8 II-CAGT
Ampicillin and Kanamycin
Plate 4 / D / 9 II-CATA
Ampicillin and Kanamycin
Plate 4 / D / 10 II-CATC
Ampicillin and Kanamycin
Plate 4 / D / 11 II-CATG
Ampicillin and Kanamycin
Plate 4 / D / 12 II-CATT
Ampicillin and Kanamycin
Plate 4 / E / 1 II-CCAA
Ampicillin and Kanamycin
Plate 4 / E / 2 II-CCAC
Ampicillin and Kanamycin
Plate 4 / E / 3 II-CCAG
Ampicillin and Kanamycin
Plate 4 / E / 4 II-CCAT
Ampicillin and Kanamycin
Plate 4 / E / 5 II-CCCA
Ampicillin and Kanamycin
Plate 4 / E / 6 II-CCCC
Ampicillin and Kanamycin
Plate 4 / E / 7 II-CCCG
Ampicillin and Kanamycin
Plate 4 / E / 8 II-CCCT
Ampicillin and Kanamycin
Plate 4 / E / 9 II-CCGA
Ampicillin and Kanamycin
Plate 4 / E / 10 II-CCGC
Ampicillin and Kanamycin
Plate 4 / E / 11 II-CCGG
Ampicillin and Kanamycin
Plate 4 / E / 12 II-CCGT
Ampicillin and Kanamycin
Plate 4 / F / 1 II-CCTA
Ampicillin and Kanamycin
Plate 4 / F / 2 II-CCTC
Ampicillin and Kanamycin
Plate 4 / F / 3 II-CCTG
Ampicillin and Kanamycin
Plate 4 / F / 4 II-CCTT
Ampicillin and Kanamycin
Plate 4 / F / 5 II-CGAA
Ampicillin and Kanamycin
Plate 4 / F / 6 II-CGAC
Ampicillin and Kanamycin
Plate 4 / F / 7 II-CGAG
Ampicillin and Kanamycin
Plate 4 / F / 8 II-CGAT
Ampicillin and Kanamycin
Plate 4 / F / 9 II-CGCA
Ampicillin and Kanamycin
Plate 4 / F / 10 II-CGCC
Ampicillin and Kanamycin
Plate 4 / F / 11 II-CGCG
Ampicillin and Kanamycin
Plate 4 / F / 12 II-CGCT
Ampicillin and Kanamycin
Plate 4 / G / 1 II-CGGA
Ampicillin and Kanamycin
Plate 4 / G / 2 II-CGGC
Ampicillin and Kanamycin
Plate 4 / G / 3 II-CGGG
Ampicillin and Kanamycin
Plate 4 / G / 4 II-CGGT
Ampicillin and Kanamycin
Plate 4 / G / 5 II-CGTA
Ampicillin and Kanamycin
Plate 4 / G / 6 II-CGTC
Ampicillin and Kanamycin
Plate 4 / G / 7 II-CGTG
Ampicillin and Kanamycin
Plate 4 / G / 8 II-CGTT
Ampicillin and Kanamycin
Plate 4 / G / 9 II-CTAA
Ampicillin and Kanamycin
Plate 4 / G / 10 II-CTAC
Ampicillin and Kanamycin
Plate 4 / G / 11 II-CTAG
Ampicillin and Kanamycin
Plate 4 / G / 12 II-CTAT
Ampicillin and Kanamycin
Plate 4 / H / 1 II-CTCA
Ampicillin and Kanamycin
Plate 4 / H / 2 II-CTCC
Ampicillin and Kanamycin
Plate 4 / H / 3 II-CTCG
Ampicillin and Kanamycin
Plate 4 / H / 4 II-CTCT
Ampicillin and Kanamycin
Plate 4 / H / 5 II-CTGA
Ampicillin and Kanamycin
Plate 4 / H / 6 II-CTGC
Ampicillin and Kanamycin
Plate 4 / H / 7 II-CTGG
Ampicillin and Kanamycin
Plate 4 / H / 8 II-CTGT
Ampicillin and Kanamycin
Plate 4 / H / 9 II-CTTA
Ampicillin and Kanamycin
Plate 4 / H / 10 II-CTTC
Ampicillin and Kanamycin
Plate 4 / H / 11 II-CTTG
Ampicillin and Kanamycin
Plate 4 / H / 12 II-CTTT
Ampicillin and Kanamycin
Plate 5 / A / 1 II-GAAA
Ampicillin and Kanamycin
Plate 5 / A / 2 II-GAAC
Ampicillin and Kanamycin
Plate 5 / A / 3 II-GAAG
Ampicillin and Kanamycin
Plate 5 / A / 4 II-GAAT
Ampicillin and Kanamycin
Plate 5 / A / 5 II-GACA
Ampicillin and Kanamycin
Plate 5 / A / 6 II-GACC
Ampicillin and Kanamycin
Plate 5 / A / 7 II-GACG
Ampicillin and Kanamycin
Plate 5 / A / 8 II-GACT
Ampicillin and Kanamycin
Plate 5 / A / 9 II-GAGA
Ampicillin and Kanamycin
Plate 5 / A / 10 II-GAGC
Ampicillin and Kanamycin
Plate 5 / A / 11 II-GAGG
Ampicillin and Kanamycin
Plate 5 / A / 12 II-GAGT
Ampicillin and Kanamycin
Plate 5 / B / 1 II-GATA
Ampicillin and Kanamycin
Plate 5 / B / 2 II-GATC
Ampicillin and Kanamycin
Plate 5 / B / 3 II-GATG
Ampicillin and Kanamycin
Plate 5 / B / 4 II-GATT
Ampicillin and Kanamycin
Plate 5 / B / 5 II-GCAA
Ampicillin and Kanamycin
Plate 5 / B / 6 II-GCAC
Ampicillin and Kanamycin
Plate 5 / B / 7 II-GCAG
Ampicillin and Kanamycin
Plate 5 / B / 8 II-GCAT
Ampicillin and Kanamycin
Plate 5 / B / 9 II-GCCA
Ampicillin and Kanamycin
Plate 5 / B / 10 II-GCCC
Ampicillin and Kanamycin
Plate 5 / B / 11 II-GCCG
Ampicillin and Kanamycin
Plate 5 / B / 12 II-GCCT
Ampicillin and Kanamycin
Plate 5 / C / 1 II-GCGA
Ampicillin and Kanamycin
Plate 5 / C / 2 II-GCGC
Ampicillin and Kanamycin
Plate 5 / C / 3 II-GCGG
Ampicillin and Kanamycin
Plate 5 / C / 4 II-GCGT
Ampicillin and Kanamycin
Plate 5 / C / 5 II-GCTA
Ampicillin and Kanamycin
Plate 5 / C / 6 II-GCTC
Ampicillin and Kanamycin
Plate 5 / C / 7 II-GCTG
Ampicillin and Kanamycin
Plate 5 / C / 8 II-GCTT
Ampicillin and Kanamycin
Plate 5 / C / 9 II-GGAA
Ampicillin and Kanamycin
Plate 5 / C / 10 II-GGAC
Ampicillin and Kanamycin
Plate 5 / C / 11 II-GGAG
Ampicillin and Kanamycin
Plate 5 / C / 12 II-GGAT
Ampicillin and Kanamycin
Plate 5 / D / 1 II-GGCA
Ampicillin and Kanamycin
Plate 5 / D / 2 II-GGCC
Ampicillin and Kanamycin
Plate 5 / D / 3 II-GGCG
Ampicillin and Kanamycin
Plate 5 / D / 4 II-GGCT
Ampicillin and Kanamycin
Plate 5 / D / 5 II-GGGA
Ampicillin and Kanamycin
Plate 5 / D / 6 II-GGGC
Ampicillin and Kanamycin
Plate 5 / D / 7 II-GGGG
Ampicillin and Kanamycin
Plate 5 / D / 8 II-GGGT
Ampicillin and Kanamycin
Plate 5 / D / 9 II-GGTA
Ampicillin and Kanamycin
Plate 5 / D / 10 II-GGTC
Ampicillin and Kanamycin
Plate 5 / D / 11 II-GGTG
Ampicillin and Kanamycin
Plate 5 / D / 12 II-GGTT
Ampicillin and Kanamycin
Plate 5 / E / 1 II-GTAA
Ampicillin and Kanamycin
Plate 5 / E / 2 II-GTAC
Ampicillin and Kanamycin
Plate 5 / E / 3 II-GTAG
Ampicillin and Kanamycin
Plate 5 / E / 4 II-GTAT
Ampicillin and Kanamycin
Plate 5 / E / 5 II-GTCA
Ampicillin and Kanamycin
Plate 5 / E / 6 II-GTCC
Ampicillin and Kanamycin
Plate 5 / E / 7 II-GTCG
Ampicillin and Kanamycin
Plate 5 / E / 8 II-GTCT
Ampicillin and Kanamycin
Plate 5 / E / 9 II-GTGA
Ampicillin and Kanamycin
Plate 5 / E / 10 II-GTGC
Ampicillin and Kanamycin
Plate 5 / E / 11 II-GTGG
Ampicillin and Kanamycin
Plate 5 / E / 12 II-GTGT
Ampicillin and Kanamycin
Plate 5 / F / 1 II-GTTA
Ampicillin and Kanamycin
Plate 5 / F / 2 II-GTTC
Ampicillin and Kanamycin
Plate 5 / F / 3 II-GTTG
Ampicillin and Kanamycin
Plate 5 / F / 4 II-GTTT
Ampicillin and Kanamycin
Plate 5 / F / 5 II-TAAA
Ampicillin and Kanamycin
Plate 5 / F / 6 II-TAAC
Ampicillin and Kanamycin
Plate 5 / F / 7 II-TAAG
Ampicillin and Kanamycin
Plate 5 / F / 8 II-TAAT
Ampicillin and Kanamycin
Plate 5 / F / 9 II-TACA
Ampicillin and Kanamycin
Plate 5 / F / 10 II-TACC
Ampicillin and Kanamycin
Plate 5 / F / 11 II-TACG
Ampicillin and Kanamycin
Plate 5 / F / 12 II-TACT
Ampicillin and Kanamycin
Plate 5 / G / 1 II-TAGA
Ampicillin and Kanamycin
Plate 5 / G / 2 II-TAGC
Ampicillin and Kanamycin
Plate 5 / G / 3 II-TAGG
Ampicillin and Kanamycin
Plate 5 / G / 4 II-TAGT
Ampicillin and Kanamycin
Plate 5 / G / 5 II-TATA
Ampicillin and Kanamycin
Plate 5 / G / 6 II-TATC
Ampicillin and Kanamycin
Plate 5 / G / 7 II-TATG
Ampicillin and Kanamycin
Plate 5 / G / 8 II-TATT
Ampicillin and Kanamycin
Plate 5 / G / 9 II-TCAA
Ampicillin and Kanamycin
Plate 5 / G / 10 II-TCAC
Ampicillin and Kanamycin
Plate 5 / G / 11 II-TCAG
Ampicillin and Kanamycin
Plate 5 / G / 12 II-TCAT
Ampicillin and Kanamycin
Plate 5 / H / 1 II-TCCA
Ampicillin and Kanamycin
Plate 5 / H / 2 II-TCCC
Ampicillin and Kanamycin
Plate 5 / H / 3 II-TCCG
Ampicillin and Kanamycin
Plate 5 / H / 4 II-TCCT
Ampicillin and Kanamycin
Plate 5 / H / 5 II-TCGA
Ampicillin and Kanamycin
Plate 5 / H / 6 II-TCGC
Ampicillin and Kanamycin
Plate 5 / H / 7 II-TCGG
Ampicillin and Kanamycin
Plate 5 / H / 8 II-TCGT
Ampicillin and Kanamycin
Plate 5 / H / 9 II-TCTA
Ampicillin and Kanamycin
Plate 5 / H / 10 II-TCTC
Ampicillin and Kanamycin
Plate 5 / H / 11 II-TCTG
Ampicillin and Kanamycin
Plate 5 / H / 12 II-TCTT
Ampicillin and Kanamycin
Plate 6 / A / 1 II-TGAA
Ampicillin and Kanamycin
Plate 6 / A / 2 II-TGAC
Ampicillin and Kanamycin
Plate 6 / A / 3 II-TGAG
Ampicillin and Kanamycin
Plate 6 / A / 4 II-TGAT
Ampicillin and Kanamycin
Plate 6 / A / 5 II-TGCA
Ampicillin and Kanamycin
Plate 6 / A / 6 II-TGCC
Ampicillin and Kanamycin
Plate 6 / A / 7 II-TGCG
Ampicillin and Kanamycin
Plate 6 / A / 8 II-TGCT
Ampicillin and Kanamycin
Plate 6 / A / 9 II-TGGA
Ampicillin and Kanamycin
Plate 6 / A / 10 II-TGGC
Ampicillin and Kanamycin
Plate 6 / A / 11 II-TGGG
Ampicillin and Kanamycin
Plate 6 / A / 12 II-TGGT
Ampicillin and Kanamycin
Plate 6 / B / 1 II-TGTA
Ampicillin and Kanamycin
Plate 6 / B / 2 II-TGTC
Ampicillin and Kanamycin
Plate 6 / B / 3 II-TGTG
Ampicillin and Kanamycin
Plate 6 / B / 4 II-TGTT
Ampicillin and Kanamycin
Plate 6 / B / 5 II-TTAA
Ampicillin and Kanamycin
Plate 6 / B / 6 II-TTAC
Ampicillin and Kanamycin
Plate 6 / B / 7 II-TTAG
Ampicillin and Kanamycin
Plate 6 / B / 8 II-TTAT
Ampicillin and Kanamycin
Plate 6 / B / 9 II-TTCA
Ampicillin and Kanamycin
Plate 6 / B / 10 II-TTCC
Ampicillin and Kanamycin
Plate 6 / B / 11 II-TTCG
Ampicillin and Kanamycin
Plate 6 / B / 12 II-TTCT
Ampicillin and Kanamycin
Plate 6 / C / 1 II-TTGA
Ampicillin and Kanamycin
Plate 6 / C / 2 II-TTGC
Ampicillin and Kanamycin
Plate 6 / C / 3 II-TTGG
Ampicillin and Kanamycin
Plate 6 / C / 4 II-TTGT
Ampicillin and Kanamycin
Plate 6 / C / 5 II-TTTA
Ampicillin and Kanamycin
Plate 6 / C / 6 II-TTTC
Ampicillin and Kanamycin
Plate 6 / C / 7 II-TTTG
Ampicillin and Kanamycin
Plate 6 / C / 8 II-TTTT
Ampicillin and Kanamycin
Plate 6 / C / 9 III-AAAA
Ampicillin and Kanamycin
Plate 6 / C / 10 III-AAAC
Ampicillin and Kanamycin
Plate 6 / C / 11 III-AAAG
Ampicillin and Kanamycin
Plate 6 / C / 12 III-AAAT
Ampicillin and Kanamycin
Plate 6 / D / 1 III-AACA
Ampicillin and Kanamycin
Plate 6 / D / 2 III-AACC
Ampicillin and Kanamycin
Plate 6 / D / 3 III-AACG
Ampicillin and Kanamycin
Plate 6 / D / 4 III-AACT
Ampicillin and Kanamycin
Plate 6 / D / 5 III-AAGA
Ampicillin and Kanamycin
Plate 6 / D / 6 III-AAGC
Ampicillin and Kanamycin
Plate 6 / D / 7 III-AAGG
Ampicillin and Kanamycin
Plate 6 / D / 8 III-AAGT
Ampicillin and Kanamycin
Plate 6 / D / 9 III-AATA
Ampicillin and Kanamycin
Plate 6 / D / 10 III-AATC
Ampicillin and Kanamycin
Plate 6 / D / 11 III-AATG
Ampicillin and Kanamycin
Plate 6 / D / 12 III-AATT
Ampicillin and Kanamycin
Plate 6 / E / 1 III-ACAA
Ampicillin and Kanamycin
Plate 6 / E / 2 III-ACAC
Ampicillin and Kanamycin
Plate 6 / E / 3 III-ACAG
Ampicillin and Kanamycin
Plate 6 / E / 4 III-ACAT
Ampicillin and Kanamycin
Plate 6 / E / 5 III-ACCA
Ampicillin and Kanamycin
Plate 6 / E / 6 III-ACCC
Ampicillin and Kanamycin
Plate 6 / E / 7 III-ACCG
Ampicillin and Kanamycin
Plate 6 / E / 8 III-ACCT
Ampicillin and Kanamycin
Plate 6 / E / 9 III-ACGA
Ampicillin and Kanamycin
Plate 6 / E / 10 III-ACGC
Ampicillin and Kanamycin
Plate 6 / E / 11 III-ACGG
Ampicillin and Kanamycin
Plate 6 / E / 12 III-ACGT
Ampicillin and Kanamycin
Plate 6 / F / 1 III-ACTA
Ampicillin and Kanamycin
Plate 6 / F / 2 III-ACTC
Ampicillin and Kanamycin
Plate 6 / F / 3 III-ACTG
Ampicillin and Kanamycin
Plate 6 / F / 4 III-ACTT
Ampicillin and Kanamycin
Plate 6 / F / 5 III-AGAA
Ampicillin and Kanamycin
Plate 6 / F / 6 III-AGAC
Ampicillin and Kanamycin
Plate 6 / F / 7 III-AGAG
Ampicillin and Kanamycin
Plate 6 / F / 8 III-AGAT
Ampicillin and Kanamycin
Plate 6 / F / 9 III-AGCA
Ampicillin and Kanamycin
Plate 6 / F / 10 III-AGCC
Ampicillin and Kanamycin
Plate 6 / F / 11 III-AGCG
Ampicillin and Kanamycin
Plate 6 / F / 12 III-AGCT
Ampicillin and Kanamycin
Plate 6 / G / 1 III-AGGA
Ampicillin and Kanamycin
Plate 6 / G / 2 III-AGGC
Ampicillin and Kanamycin
Plate 6 / G / 3 III-AGGG
Ampicillin and Kanamycin
Plate 6 / G / 4 III-AGGT
Ampicillin and Kanamycin
Plate 6 / G / 5 III-AGTA
Ampicillin and Kanamycin
Plate 6 / G / 6 III-AGTC
Ampicillin and Kanamycin
Plate 6 / G / 7 III-AGTG
Ampicillin and Kanamycin
Plate 6 / G / 8 III-AGTT
Ampicillin and Kanamycin
Plate 6 / G / 9 III-ATAA
Ampicillin and Kanamycin
Plate 6 / G / 10 III-ATAC
Ampicillin and Kanamycin
Plate 6 / G / 11 III-ATAG
Ampicillin and Kanamycin
Plate 6 / G / 12 III-ATAT
Ampicillin and Kanamycin
Plate 6 / H / 1 III-ATCA
Ampicillin and Kanamycin
Plate 6 / H / 2 III-ATCC
Ampicillin and Kanamycin
Plate 6 / H / 3 III-ATCG
Ampicillin and Kanamycin
Plate 6 / H / 4 III-ATCT
Ampicillin and Kanamycin
Plate 6 / H / 5 III-ATGA
Ampicillin and Kanamycin
Plate 6 / H / 6 III-ATGC
Ampicillin and Kanamycin
Plate 6 / H / 7 III-ATGG
Ampicillin and Kanamycin
Plate 6 / H / 8 III-ATGT
Ampicillin and Kanamycin
Plate 6 / H / 9 III-ATTA
Ampicillin and Kanamycin
Plate 6 / H / 10 III-ATTC
Ampicillin and Kanamycin
Plate 6 / H / 11 III-ATTG
Ampicillin and Kanamycin
Plate 6 / H / 12 III-ATTT
Ampicillin and Kanamycin
Plate 7 / A / 1 III-CAAA
Ampicillin and Kanamycin
Plate 7 / A / 2 III-CAAC
Ampicillin and Kanamycin
Plate 7 / A / 3 III-CAAG
Ampicillin and Kanamycin
Plate 7 / A / 4 III-CAAT
Ampicillin and Kanamycin
Plate 7 / A / 5 III-CACA
Ampicillin and Kanamycin
Plate 7 / A / 6 III-CACC
Ampicillin and Kanamycin
Plate 7 / A / 7 III-CACG
Ampicillin and Kanamycin
Plate 7 / A / 8 III-CACT
Ampicillin and Kanamycin
Plate 7 / A / 9 III-CAGA
Ampicillin and Kanamycin
Plate 7 / A / 10 III-CAGC
Ampicillin and Kanamycin
Plate 7 / A / 11 III-CAGG
Ampicillin and Kanamycin
Plate 7 / A / 12 III-CAGT
Ampicillin and Kanamycin
Plate 7 / B / 1 III-CATA
Ampicillin and Kanamycin
Plate 7 / B / 2 III-CATC
Ampicillin and Kanamycin
Plate 7 / B / 3 III-CATG
Ampicillin and Kanamycin
Plate 7 / B / 4 III-CATT
Ampicillin and Kanamycin
Plate 7 / B / 5 III-CCAA
Ampicillin and Kanamycin
Plate 7 / B / 6 III-CCAC
Ampicillin and Kanamycin
Plate 7 / B / 7 III-CCAG
Ampicillin and Kanamycin
Plate 7 / B / 8 III-CCAT
Ampicillin and Kanamycin
Plate 7 / B / 9 III-CCCA
Ampicillin and Kanamycin
Plate 7 / B / 10 III-CCCC
Ampicillin and Kanamycin
Plate 7 / B / 11 III-CCCG
Ampicillin and Kanamycin
Plate 7 / B / 12 III-CCCT
Ampicillin and Kanamycin
Plate 7 / C / 1 III-CCGA
Ampicillin and Kanamycin
Plate 7 / C / 2 III-CCGC
Ampicillin and Kanamycin
Plate 7 / C / 3 III-CCGG
Ampicillin and Kanamycin
Plate 7 / C / 4 III-CCGT
Ampicillin and Kanamycin
Plate 7 / C / 5 III-CCTA
Ampicillin and Kanamycin
Plate 7 / C / 6 III-CCTC
Ampicillin and Kanamycin
Plate 7 / C / 7 III-CCTG
Ampicillin and Kanamycin
Plate 7 / C / 8 III-CCTT
Ampicillin and Kanamycin
Plate 7 / C / 9 III-CGAA
Ampicillin and Kanamycin
Plate 7 / C / 10 III-CGAC
Ampicillin and Kanamycin
Plate 7 / C / 11 III-CGAG
Ampicillin and Kanamycin
Plate 7 / C / 12 III-CGAT
Ampicillin and Kanamycin
Plate 7 / D / 1 III-CGCA
Ampicillin and Kanamycin
Plate 7 / D / 2 III-CGCC
Ampicillin and Kanamycin
Plate 7 / D / 3 III-CGCG
Ampicillin and Kanamycin
Plate 7 / D / 4 III-CGCT
Ampicillin and Kanamycin
Plate 7 / D / 5 III-CGGA
Ampicillin and Kanamycin
Plate 7 / D / 6 III-CGGC
Ampicillin and Kanamycin
Plate 7 / D / 7 III-CGGG
Ampicillin and Kanamycin
Plate 7 / D / 8 III-CGGT
Ampicillin and Kanamycin
Plate 7 / D / 9 III-CGTA
Ampicillin and Kanamycin
Plate 7 / D / 10 III-CGTC
Ampicillin and Kanamycin
Plate 7 / D / 11 III-CGTG
Ampicillin and Kanamycin
Plate 7 / D / 12 III-CGTT
Ampicillin and Kanamycin
Plate 7 / E / 1 III-CTAA
Ampicillin and Kanamycin
Plate 7 / E / 2 III-CTAC
Ampicillin and Kanamycin
Plate 7 / E / 3 III-CTAG
Ampicillin and Kanamycin
Plate 7 / E / 4 III-CTAT
Ampicillin and Kanamycin
Plate 7 / E / 5 III-CTCA
Ampicillin and Kanamycin
Plate 7 / E / 6 III-CTCC
Ampicillin and Kanamycin
Plate 7 / E / 7 III-CTCG
Ampicillin and Kanamycin
Plate 7 / E / 8 III-CTCT
Ampicillin and Kanamycin
Plate 7 / E / 9 III-CTGA
Ampicillin and Kanamycin
Plate 7 / E / 10 III-CTGC
Ampicillin and Kanamycin
Plate 7 / E / 11 III-CTGG
Ampicillin and Kanamycin
Plate 7 / E / 12 III-CTGT
Ampicillin and Kanamycin
Plate 7 / F / 1 III-CTTA
Ampicillin and Kanamycin
Plate 7 / F / 2 III-CTTC
Ampicillin and Kanamycin
Plate 7 / F / 3 III-CTTG
Ampicillin and Kanamycin
Plate 7 / F / 4 III-CTTT
Ampicillin and Kanamycin
Plate 7 / F / 5 III-GAAA
Ampicillin and Kanamycin
Plate 7 / F / 6 III-GAAC
Ampicillin and Kanamycin
Plate 7 / F / 7 III-GAAG
Ampicillin and Kanamycin
Plate 7 / F / 8 III-GAAT
Ampicillin and Kanamycin
Plate 7 / F / 9 III-GACA
Ampicillin and Kanamycin
Plate 7 / F / 10 III-GACC
Ampicillin and Kanamycin
Plate 7 / F / 11 III-GACG
Ampicillin and Kanamycin
Plate 7 / F / 12 III-GACT
Ampicillin and Kanamycin
Plate 7 / G / 1 III-GAGA
Ampicillin and Kanamycin
Plate 7 / G / 2 III-GAGC
Ampicillin and Kanamycin
Plate 7 / G / 3 III-GAGG
Ampicillin and Kanamycin
Plate 7 / G / 4 III-GAGT
Ampicillin and Kanamycin
Plate 7 / G / 5 III-GATA
Ampicillin and Kanamycin
Plate 7 / G / 6 III-GATC
Ampicillin and Kanamycin
Plate 7 / G / 7 III-GATG
Ampicillin and Kanamycin
Plate 7 / G / 8 III-GATT
Ampicillin and Kanamycin
Plate 7 / G / 9 III-GCAA
Ampicillin and Kanamycin
Plate 7 / G / 10 III-GCAC
Ampicillin and Kanamycin
Plate 7 / G / 11 III-GCAG
Ampicillin and Kanamycin
Plate 7 / G / 12 III-GCAT
Ampicillin and Kanamycin
Plate 7 / H / 1 III-GCCA
Ampicillin and Kanamycin
Plate 7 / H / 2 III-GCCC
Ampicillin and Kanamycin
Plate 7 / H / 3 III-GCCG
Ampicillin and Kanamycin
Plate 7 / H / 4 III-GCCT
Ampicillin and Kanamycin
Plate 7 / H / 5 III-GCGA
Ampicillin and Kanamycin
Plate 7 / H / 6 III-GCGC
Ampicillin and Kanamycin
Plate 7 / H / 7 III-GCGG
Ampicillin and Kanamycin
Plate 7 / H / 8 III-GCGT
Ampicillin and Kanamycin
Plate 7 / H / 9 III-GCTA
Ampicillin and Kanamycin
Plate 7 / H / 10 III-GCTC
Ampicillin and Kanamycin
Plate 7 / H / 11 III-GCTG
Ampicillin and Kanamycin
Plate 7 / H / 12 III-GCTT
Ampicillin and Kanamycin
Plate 8 / A / 1 III-GGAA
Ampicillin and Kanamycin
Plate 8 / A / 2 III-GGAC
Ampicillin and Kanamycin
Plate 8 / A / 3 III-GGAG
Ampicillin and Kanamycin
Plate 8 / A / 4 III-GGAT
Ampicillin and Kanamycin
Plate 8 / A / 5 III-GGCA
Ampicillin and Kanamycin
Plate 8 / A / 6 III-GGCC
Ampicillin and Kanamycin
Plate 8 / A / 7 III-GGCG
Ampicillin and Kanamycin
Plate 8 / A / 8 III-GGCT
Ampicillin and Kanamycin
Plate 8 / A / 9 III-GGGA
Ampicillin and Kanamycin
Plate 8 / A / 10 III-GGGC
Ampicillin and Kanamycin
Plate 8 / A / 11 III-GGGG
Ampicillin and Kanamycin
Plate 8 / A / 12 III-GGGT
Ampicillin and Kanamycin
Plate 8 / B / 1 III-GGTA
Ampicillin and Kanamycin
Plate 8 / B / 2 III-GGTC
Ampicillin and Kanamycin
Plate 8 / B / 3 III-GGTG
Ampicillin and Kanamycin
Plate 8 / B / 4 III-GGTT
Ampicillin and Kanamycin
Plate 8 / B / 5 III-GTAA
Ampicillin and Kanamycin
Plate 8 / B / 6 III-GTAC
Ampicillin and Kanamycin
Plate 8 / B / 7 III-GTAG
Ampicillin and Kanamycin
Plate 8 / B / 8 III-GTAT
Ampicillin and Kanamycin
Plate 8 / B / 9 III-GTCA
Ampicillin and Kanamycin
Plate 8 / B / 10 III-GTCC
Ampicillin and Kanamycin
Plate 8 / B / 11 III-GTCG
Ampicillin and Kanamycin
Plate 8 / B / 12 III-GTCT
Ampicillin and Kanamycin
Plate 8 / C / 1 III-GTGA
Ampicillin and Kanamycin
Plate 8 / C / 2 III-GTGC
Ampicillin and Kanamycin
Plate 8 / C / 3 III-GTGG
Ampicillin and Kanamycin
Plate 8 / C / 4 III-GTGT
Ampicillin and Kanamycin
Plate 8 / C / 5 III-GTTA
Ampicillin and Kanamycin
Plate 8 / C / 6 III-GTTC
Ampicillin and Kanamycin
Plate 8 / C / 7 III-GTTG
Ampicillin and Kanamycin
Plate 8 / C / 8 III-GTTT
Ampicillin and Kanamycin
Plate 8 / C / 9 III-TAAA
Ampicillin and Kanamycin
Plate 8 / C / 10 III-TAAC
Ampicillin and Kanamycin
Plate 8 / C / 11 III-TAAG
Ampicillin and Kanamycin
Plate 8 / C / 12 III-TAAT
Ampicillin and Kanamycin
Plate 8 / D / 1 III-TACA
Ampicillin and Kanamycin
Plate 8 / D / 2 III-TACC
Ampicillin and Kanamycin
Plate 8 / D / 3 III-TACG
Ampicillin and Kanamycin
Plate 8 / D / 4 III-TACT
Ampicillin and Kanamycin
Plate 8 / D / 5 III-TAGA
Ampicillin and Kanamycin
Plate 8 / D / 6 III-TAGC
Ampicillin and Kanamycin
Plate 8 / D / 7 III-TAGG
Ampicillin and Kanamycin
Plate 8 / D / 8 III-TAGT
Ampicillin and Kanamycin
Plate 8 / D / 9 III-TATA
Ampicillin and Kanamycin
Plate 8 / D / 10 III-TATC
Ampicillin and Kanamycin
Plate 8 / D / 11 III-TATG
Ampicillin and Kanamycin
Plate 8 / D / 12 III-TATT
Ampicillin and Kanamycin
Plate 8 / E / 1 III-TCAA
Ampicillin and Kanamycin
Plate 8 / E / 2 III-TCAC
Ampicillin and Kanamycin
Plate 8 / E / 3 III-TCAG
Ampicillin and Kanamycin
Plate 8 / E / 4 III-TCAT
Ampicillin and Kanamycin
Plate 8 / E / 5 III-TCCA
Ampicillin and Kanamycin
Plate 8 / E / 6 III-TCCC
Ampicillin and Kanamycin
Plate 8 / E / 7 III-TCCG
Ampicillin and Kanamycin
Plate 8 / E / 8 III-TCCT
Ampicillin and Kanamycin
Plate 8 / E / 9 III-TCGA
Ampicillin and Kanamycin
Plate 8 / E / 10 III-TCGC
Ampicillin and Kanamycin
Plate 8 / E / 11 III-TCGG
Ampicillin and Kanamycin
Plate 8 / E / 12 III-TCGT
Ampicillin and Kanamycin
Plate 8 / F / 1 III-TCTA
Ampicillin and Kanamycin
Plate 8 / F / 2 III-TCTC
Ampicillin and Kanamycin
Plate 8 / F / 3 III-TCTG
Ampicillin and Kanamycin
Plate 8 / F / 4 III-TCTT
Ampicillin and Kanamycin
Plate 8 / F / 5 III-TGAA
Ampicillin and Kanamycin
Plate 8 / F / 6 III-TGAC
Ampicillin and Kanamycin
Plate 8 / F / 7 III-TGAG
Ampicillin and Kanamycin
Plate 8 / F / 8 III-TGAT
Ampicillin and Kanamycin
Plate 8 / F / 9 III-TGCA
Ampicillin and Kanamycin
Plate 8 / F / 10 III-TGCC
Ampicillin and Kanamycin
Plate 8 / F / 11 III-TGCG
Ampicillin and Kanamycin
Plate 8 / F / 12 III-TGCT
Ampicillin and Kanamycin
Plate 8 / G / 1 III-TGGA
Ampicillin and Kanamycin
Plate 8 / G / 2 III-TGGC
Ampicillin and Kanamycin
Plate 8 / G / 3 III-TGGG
Ampicillin and Kanamycin
Plate 8 / G / 4 III-TGGT
Ampicillin and Kanamycin
Plate 8 / G / 5 III-TGTA
Ampicillin and Kanamycin
Plate 8 / G / 6 III-TGTC
Ampicillin and Kanamycin
Plate 8 / G / 7 III-TGTG
Ampicillin and Kanamycin
Plate 8 / G / 8 III-TGTT
Ampicillin and Kanamycin
Plate 8 / G / 9 III-TTAA
Ampicillin and Kanamycin
Plate 8 / G / 10 III-TTAC
Ampicillin and Kanamycin
Plate 8 / G / 11 III-TTAG
Ampicillin and Kanamycin
Plate 8 / G / 12 III-TTAT
Ampicillin and Kanamycin
Plate 8 / H / 1 III-TTCA
Ampicillin and Kanamycin
Plate 8 / H / 2 III-TTCC
Ampicillin and Kanamycin
Plate 8 / H / 3 III-TTCG
Ampicillin and Kanamycin
Plate 8 / H / 4 III-TTCT
Ampicillin and Kanamycin
Plate 8 / H / 5 III-TTGA
Ampicillin and Kanamycin
Plate 8 / H / 6 III-TTGC
Ampicillin and Kanamycin
Plate 8 / H / 7 III-TTGG
Ampicillin and Kanamycin
Plate 8 / H / 8 III-TTGT
Ampicillin and Kanamycin
Plate 8 / H / 9 III-TTTA
Ampicillin and Kanamycin
Plate 8 / H / 10 III-TTTC
Ampicillin and Kanamycin
Plate 8 / H / 11 III-TTTG
Ampicillin and Kanamycin
Plate 8 / H / 12 III-TTTT
Ampicillin and Kanamycin
Plate 9 / A / 1 IV-AAA
Ampicillin and Kanamycin
Plate 9 / A / 2 IV-AAC
Ampicillin and Kanamycin
Plate 9 / A / 3 IV-AAG
Ampicillin and Kanamycin
Plate 9 / A / 4 IV-AAT
Ampicillin and Kanamycin
Plate 9 / A / 5 IV-ACA
Ampicillin and Kanamycin
Plate 9 / A / 6 IV-ACC
Ampicillin and Kanamycin
Plate 9 / A / 7 IV-ACG
Ampicillin and Kanamycin
Plate 9 / A / 8 IV-ACT
Ampicillin and Kanamycin
Plate 9 / A / 9 IV-AGA
Ampicillin and Kanamycin
Plate 9 / A / 10 IV-AGC
Ampicillin and Kanamycin
Plate 9 / A / 11 IV-AGG
Ampicillin and Kanamycin
Plate 9 / A / 12 IV-AGT
Ampicillin and Kanamycin
Plate 9 / B / 1 IV-ATA
Ampicillin and Kanamycin
Plate 9 / B / 2 IV-ATC
Ampicillin and Kanamycin
Plate 9 / B / 3 IV-ATG
Ampicillin and Kanamycin
Plate 9 / B / 4 IV-ATT
Ampicillin and Kanamycin
Plate 9 / B / 5 IV-CAA
Ampicillin and Kanamycin
Plate 9 / B / 6 IV-CAC
Ampicillin and Kanamycin
Plate 9 / B / 7 IV-CAG
Ampicillin and Kanamycin
Plate 9 / B / 8 IV-CAT
Ampicillin and Kanamycin
Plate 9 / B / 9 IV-CCA
Ampicillin and Kanamycin
Plate 9 / B / 10 IV-CCC
Ampicillin and Kanamycin
Plate 9 / B / 11 IV-CCG
Ampicillin and Kanamycin
Plate 9 / B / 12 IV-CCT
Ampicillin and Kanamycin
Plate 9 / C / 1 IV-CGA
Ampicillin and Kanamycin
Plate 9 / C / 2 IV-CGC
Ampicillin and Kanamycin
Plate 9 / C / 3 IV-CGG
Ampicillin and Kanamycin
Plate 9 / C / 4 IV-CGT
Ampicillin and Kanamycin
Plate 9 / C / 5 IV-CTA
Ampicillin and Kanamycin
Plate 9 / C / 6 IV-CTC
Ampicillin and Kanamycin
Plate 9 / C / 7 IV-CTG
Ampicillin and Kanamycin
Plate 9 / C / 8 IV-CTT
Ampicillin and Kanamycin
Plate 9 / C / 9 IV-GAA
Ampicillin and Kanamycin
Plate 9 / C / 10 IV-GAC
Ampicillin and Kanamycin
Plate 9 / C / 11 IV-GAG
Ampicillin and Kanamycin
Plate 9 / C / 12 IV-GAT
Ampicillin and Kanamycin
Plate 9 / D / 1 IV-GCA
Ampicillin and Kanamycin
Plate 9 / D / 2 IV-GCC
Ampicillin and Kanamycin
Plate 9 / D / 3 IV-GCG
Ampicillin and Kanamycin
Plate 9 / D / 4 IV-GCT
Ampicillin and Kanamycin
Plate 9 / D / 5 IV-GGA
Ampicillin and Kanamycin
Plate 9 / D / 6 IV-GGC
Ampicillin and Kanamycin
Plate 9 / D / 7 IV-GGG
Ampicillin and Kanamycin
Plate 9 / D / 8 IV-GGT
Ampicillin and Kanamycin
Plate 9 / D / 9 IV-GTA
Ampicillin and Kanamycin
Plate 9 / D / 10 IV-GTC
Ampicillin and Kanamycin
Plate 9 / D / 11 IV-GTG
Ampicillin and Kanamycin
Plate 9 / D / 12 IV-GTT
Ampicillin and Kanamycin
Plate 9 / E / 1 IV-TAA
Ampicillin and Kanamycin
Plate 9 / E / 2 IV-TAC
Ampicillin and Kanamycin
Plate 9 / E / 3 IV-TAG
Ampicillin and Kanamycin
Plate 9 / E / 4 IV-TAT
Ampicillin and Kanamycin
Plate 9 / E / 5 IV-TCA
Ampicillin and Kanamycin
Plate 9 / E / 6 IV-TCC
Ampicillin and Kanamycin
Plate 9 / E / 7 IV-TCG
Ampicillin and Kanamycin
Plate 9 / E / 8 IV-TCT
Ampicillin and Kanamycin
Plate 9 / E / 9 IV-TGA
Ampicillin and Kanamycin
Plate 9 / E / 10 IV-TGC
Ampicillin and Kanamycin
Plate 9 / E / 11 mCerulean3-Keratin-17
Kanamycin
Plate 9 / E / 12 mCerulean3-H2B-C-10
Kanamycin
Plate 9 / F / 1 mCerulean3-Vimentin-7
Kanamycin
Plate 9 / F / 2 mCerulean3-Alpha Actinin-19
Kanamycin
Plate 9 / F / 3 mCerulean3-EB3-7
Kanamycin
Plate 9 / F / 4 cmv 16 E7 68CVQ
Ampicillin
Data calculated @ 2016-12-04

Kit Plate Map - #1000000034