Engineered TALENs for the EGFP Reporter Gene
The Joung Lab recently used their high-throughput FLASH method to construct a large series of engineered TALENs targeted to 48 sites in the EGFP reporter gene (Reyon & Tsai et al., Nat Biotechnol. 2012 ). The table below lists plasmids encoding these 48 pairs of TALENs that were shown to be active at their target sites on an integrated EGFP reporter gene in cultured U2OS cells.
| EGFP TALEN Plasmid | Plasmid Name | Target Half-Site |
|---|---|---|
| eGFP-TALEN-01-Left | TAL2000 | TCGCCACCAT |
| eGFP-TALEN-01-Right | TAL2001 | TGAACAGCTC |
| eGFP-TALEN-02-Left | TAL2002 | TGGTGCCCAT |
| eGFP-TALEN-02-Right | TAL2003 | TTACGTCGCC |
| eGFP-TALEN-03-Left | TAL2004 | TCTGCACCAC |
| eGFP-TALEN-03-Right | TAL2005 | TGGGCCAGGG |
| eGFP-TALEN-04-Left | TAL2006 | TGGAGTACAA |
| eGFP-TALEN-04-Right | TAL2007 | TGATATAGAC |
| eGFP-TALEN-05-Left | TAL2008 | TTCAGCGTGTC |
| eGFP-TALEN-05-Right | TAL2009 | TAGGTGGCATC |
| eGFP-TALEN-06-Left | TAL2010 | TGCCACCTACG |
| eGFP-TALEN-06-Right | TAL2011 | TGCAGATGAAC |
| eGFP-TALEN-07-Left | TAL2012 | TGGCCCACCCT |
| eGFP-TALEN-07-Right | TAL2013 | TGCACGCCGTA |
| eGFP-TALEN-08-Left | TAL2014 | TTCAAGATCCG |
| eGFP-TALEN-08-Right | TAL2015 | TGCACGCTGCC |
| eGFP-TALEN-09-Left | TAL2016 | TAGAGGATCCAC |
| eGFP-TALEN-09-Right | TAL2017 | TCGCCCTTGCTC |
| eGFP-TALEN-10-Left | TAL2018 | TCCGGCGAGGGC |
| eGFP-TALEN-10-Right | TAL2019 | TCAGCTTGCCGT |
| eGFP-TALEN-11-Left | TAL2020 | TGACCTACGGCG |
| eGFP-TALEN-11-Right | TAL2021 | TGGTCGGGGTAG |
| eGFP-TALEN-12-Left | TAL2022 | TCCGCCACAACA |
| eGFP-TALEN-12-Right | TAL2023 | TCGGCGAGCTGC |
| eGFP-TALEN-13-Left | TAL2024 | TCCTGGTCGAGCT |
| eGFP-TALEN-13-Right | TAL2025 | TGAACTTGTGGCC |
| eGFP-TALEN-14-Left | TAL2026 | TCAGCCGCTACCC |
| eGFP-TALEN-14-Right | TAL2027 | TGAAGAAGTCGTG |
| eGFP-TALEN-15-Left | TAL2028 | TCTTCAAGTCCGC |
| eGFP-TALEN-15-Right | TAL2029 | TGCGCTCCTGGAC |
| eGFP-TALEN-16-Left | TAL2030 | TCAAGGAGGACGG |
| eGFP-TALEN-16-Right | TAL2031 | TGTACTCCAGCTT |
| eGFP-TALEN-17-Left | TAL2032 | TCAAGGTGAACTT |
| eGFP-TALEN-17-Right | TAL2033 | TGCCGTCCTCGAT |
| eGFP-TALEN-18-Left | TAL2034 | TCCACCGGTCGCCA |
| eGFP-TALEN-18-Right | TAL2035 | TGAACAGCTCCTCG |
| eGFP-TALEN-19-Left | TAL2036 | TTCAGCGTGTCCGG |
| eGFP-TALEN-19-Right | TAL2037 | TTGCCGTAGGTGGC |
| eGFP-TALEN-20-Left | TAL2038 | TTCAGCCGCTACCC |
| eGFP-TALEN-20-Right | TAL2039 | TTGAAGAAGTCGTG |
| eGFP-TALEN-21-Left | TAL2040 | TACAACAGCCACAA |
| eGFP-TALEN-21-Right | TAL2041 | TTCTTCTGCTTGTC |
| eGFP-TALEN-22-Left | TAL2042 | TGGTGCCCATCCTGG |
| eGFP-TALEN-22-Right | TAL2043 | TTGTGGCCGTTTACG |
| eGFP-TALEN-23-Left | TAL2044 | TGCCCGAAGGCTACG |
| eGFP-TALEN-23-Right | TAL2045 | TCGTCCTTGAAGAAG |
| eGFP-TALEN-24-Left | TAL2046 | TGAACCGCATCGAGC |
| eGFP-TALEN-24-Right | TAL2047 | TTGCCGTCCTCCTTG |
| eGFP-TALEN-25-Left | TAL2048 | TCAAGATCCGCCACA |
| eGFP-TALEN-25-Right | TAL2049 | TCGGCGAGCTGCACG |
| eGFP-TALEN-26-Left | TAL2050 | TGTTCACCGGGGTGGT |
| eGFP-TALEN-26-Right | TAL2051 | TTACGTCGCCGTCCAG |
| eGFP-TALEN-27-Left | TAL2052 | TGCCCATCCTGGTCGA |
| eGFP-TALEN-27-Right | TAL2053 | TGAACTTGTGGCCGTT |
| eGFP-TALEN-28-Left | TAL2054 | TGTCCGGCGAGGGCGA |
| eGFP-TALEN-28-Right | TAL2055 | TCAGGGTCAGCTTGCC |
| eGFP-TALEN-29-Left | TAL2056 | TCATCTGCACCACCGG |
| eGFP-TALEN-29-Right | TAL2057 | TCACGAGGGTGGGCCA |
| eGFP-TALEN-30-Left | TAL2058 | TCTATATCATGGCCGA |
| eGFP-TALEN-30-Right | TAL2059 | TGAAGTTCACCTTGAT |
| eGFP-TALEN-31-Left | TAL2060 | TACGGCGTGCAGTGCTT |
| eGFP-TALEN-31-Right | TAL2061 | TCGTGCTGCTTCATGTG |
| eGFP-TALEN-32-Left | TAL2086 | TACCCCGACCACATGAA |
| eGFP-TALEN-32-Right | TAL2087 | TCGGGCATGGCGGACTT |
| eGFP-TALEN-33-Left | TAL2062 | TCCGCCATGCCCGAAGG |
| eGFP-TALEN-33-Right | TAL2063 | TCCTTGAAGAAGATGGT |
| eGFP-TALEN-34-Left | TAL2090 | TTCAAGGAGGACGGCAA |
| eGFP-TALEN-34-Right | TAL2091 | TTGTAGTTGTACTCCAG |
| eGFP-TALEN-35-Left | TAL2092 | TACAACTACAACAGCCA |
| eGFP-TALEN-35-Right | TAL2093 | TTCTTCTGCTTGTCGGC |
| eGFP-TALEN-36-Left | TAL2094 | TCACCGGGGTGGTGCCCA |
| eGFP-TALEN-36-Right | TAL2095 | TGGCCGTTTACGTCGCCG |
| eGFP-TALEN-37-Left | TAL2096 | TAAACGGCCACAAGTTCA |
| eGFP-TALEN-37-Right | TAL2097 | TAGGTGGCATCGCCCTCG |
| eGFP-TALEN-38-Left | TAL2098 | TGCAGTGCTTCAGCCGCT |
| eGFP-TALEN-38-Right | TAL2099 | TTGAAGAAGTCGTGCTGC |
| eGFP-TALEN-39-Left | TAL2064 | TCAGCGTGTCCGGCGAGGG |
| eGFP-TALEN-39-Right | TAL2065 | TCAGGGTCAGCTTGCCGTA |
| eGFP-TALEN-40-Left | TAL2066 | TGAAGTTCATCTGCACCAC |
| eGFP-TALEN-40-Right | TAL2067 | TCACGAGGGTGGGCCAGGG |
| eGFP-TALEN-41-Left | TAL2068 | TCGTGACCACCCTGACCTA |
| eGFP-TALEN-41-Right | TAL2069 | TGTGGTCGGGGTAGCGGCT |
| eGFP-TALEN-42-Left | TAL2070 | TCATGGCCGACAAGCAGAA |
| eGFP-TALEN-42-Right | TAL2071 | TGTGGCGGATCTTGAAGTT |
| eGFP-TALEN-43-Left | TAL2072 | TTCACCGGGGTGGTGCCCAT |
| eGFP-TALEN-43-Right | TAL2073 | TTGTGGCCGTTTACGTCGCC |
| eGFP-TALEN-44-Left | TAL2074 | TGCACCACCGGCAAGCTGCC |
| eGFP-TALEN-44-Right | TAL2075 | TAGGTCAGGGTGGTCACGAG |
| eGFP-TALEN-45-Left | TAL2076 | TTCAAGTCCGCCATGCCCGA |
| eGFP-TALEN-45-Right | TAL2077 | TCCTTGAAGAAGATGGTGCG |
| eGFP-TALEN-46-Left | TAL2078 | TATATCATGGCCGACAAGCA |
| eGFP-TALEN-46-Right | TAL2079 | TGGCGGATCTTGAAGTTCAC |
| eGFP-TALEN-47-Left | TAL2080 | TTCATCTGCACCACCGGCAAG |
| eGFP-TALEN-47-Right | TAL2081 | TCAGGGTGGTCACGAGGGTGG |
| eGFP-TALEN-48-Left | TAL2084 | TGAAGTTCGAGGGCGACACCC |
| eGFP-TALEN-48-Right | TAL2085 | TTGAAGTCGATGCCCTTCAGC |