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TALEngineering icon

Joung Lab REAL Assembly TALEN kit
(Kit # 1000000017 )

This 32 plasmid TALEN kit is used to assemble any combination of TALE repeats into arrays of any desired length using the REAL, REAL-Fast or FLASH assembly methods described by the Joung lab.

This kit consists of one 96-well plate, and will be shipped as bacterial glycerol stocks on dry ice. Samples should be frozen at -80°C immediately upon arrival. Individual plasmids can be ordered at $65 per plasmid, and will be shipped as bacterial stabs.

Add to Cart
$350 USD + shipping

Available to academics and nonprofits only.

Original Publication

Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Sanders JD, Cade L, Khayter C, Reyon D, Peterson RT, Joung KJ, Yeh JRJ. Nat Biotechnol. 2011 Aug 5;29(8):697-8. PubMed

Description

The Joung Lab has described a rapid and robust protocol for constructing TALE repeat arrays of any desired sequence, referred to as the REAL (Restriction Enzyme And Ligation) assembly method (Sander et al., Nat Biotechnol. 2011) as well as REAL-Fast and FLASH methods. The Joung lab TALEN kit utilizes a set of 28 plasmids each encoding a single TALE repeat domain and flanked by restriction sites used for ligation into longer arrays and 4 different TALEN expression vectors. DNA fragments encoding TALE repeat arrays can be easily transferred by simple cloning into one of four different TALEN expression vectors (each possessing one of the four different carboxy-terminal 0.5 TALE repeat domains). Using REAL, REAL-Fast or FLASH assemnbly methods, any combination of TALE repeats can be assembled into arrays of any desired length.

Kit Documentation

Icon Joung lab TALEN kit.pdf (368.2 KB)

Resources

More detailed information about TALE assembly, including detailed and up-to-date protocols, can be found on the TALengineering website. Users may also wish to join the TALengineering newsgroup

An upgraded version of the ZiFiT Targeter program is available to enable users to identify both dimeric TALEN and monomeric TALE transcriptional activator target sites and to simplify the construction of TALEN expression plasmids using REAL, REAL-Fast or FLASH assembly. This new version of ZiFiT Targeter enables users to search their sequences of interest for potential TALEN or TALE transcriptional activator target sites and then generates individual user-friendly graphical roadmaps for each target that guide the user through the REAL or REAL-Fast assembly process or lists of plasmid ID numbers required for FLASH assembly. The software is publicly available without registration at: http://zifit.partners.org.

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:
"The TALEN kit used for TALE assembly was a gift from Keith Joung (Addgene kit # 1000000017)"

For your Reference section:
Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Sanders JD, Cade L, Khayter C, Reyon D, Peterson RT, Joung KJ, Yeh JRJ. Nat Biotechnol. 2011 Aug 5;29(8):697-8. PubMed

Joung Lab REAL Assembly TALEN Kit - #1000000017

Resistance Color Key

Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.

Inventory

Searchable and sortable table of all plasmids in kit. The Well column links to its kit plate map on the right. The Plasmid column links to a plasmid's individual web page.

Kit Plate Map

96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.

Resistance Color Key

Ampicillin

Inventory

Well Plasmid Resistance
A / 1 TAL006
Ampicillin
A / 2 TAL007
Ampicillin
A / 3 TAL009
Ampicillin
A / 4 TAL010
Ampicillin
A / 5 TAL011
Ampicillin
A / 6 TAL012
Ampicillin
A / 7 TAL014
Ampicillin
A / 8 TAL015
Ampicillin
A / 9 TAL016
Ampicillin
A / 10 TAL017
Ampicillin
A / 11 TAL019
Ampicillin
A / 12 TAL020
Ampicillin
B / 1 TAL021
Ampicillin
B / 2 TAL022
Ampicillin
B / 3 TAL024
Ampicillin
B / 4 TAL025
Ampicillin
B / 5 TAL026
Ampicillin
B / 6 TAL027
Ampicillin
B / 7 TAL029
Ampicillin
B / 8 TAL030
Ampicillin
B / 9 TAL031
Ampicillin
B / 10 TAL032
Ampicillin
B / 11 TAL034
Ampicillin
B / 12 TAL035
Ampicillin
C / 1 TAL036
Ampicillin
C / 2 TAL037
Ampicillin
C / 3 TAL039
Ampicillin
C / 4 TAL040
Ampicillin
C / 5 JDS70
Ampicillin
C / 6 JDS71
Ampicillin
C / 7 JDS74
Ampicillin
C / 8 JDS78
Ampicillin
Data calculated @ 2016-12-06

Kit Plate Map - #1000000017

Plate #1

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A
B
C
D
E
F
G
H
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