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Protocols

Molecular Biology Protocols

Recovering plasmids from your stab culture

Verifying plasmids

Basic cloning protocol

Basic PCR protocol

Antibiotic concentrations for growing bacteria

Plasmid protocols

pLKO.1 protocol

pBABE protocol – A protocol from the Weinberg Lab is available on the pBABE puro plasmid page.

pLionII/pTiger protocol – A protocol from the Nolan Lab is available on the pLionII plasmid page.