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Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.
This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.
This vector is NOT available from Addgene.
Plasmid: lambda ZD35
Restriction digests of the clone give the following sizes (kb): EcoRI--19, 25. (ATCC staff) Cloned cDNA expressed from LTR promoter; neo expressed from polyoma promoter; no splicing site; ori of SV40 and pBR322. A cell line suitable for transfection by released plasmid is Wgd5, ATCC CRL 1817. The plasmid insert can be released by homologous recombination between the 2 LTRs after growth at low temperature in E.coli RRI(pRK248), ATCC 47036. Allows expression of cloned cDNA in mammalian cells; can be used for transduction. Allows plasmid replication in mouse (WOP) and monkey (COS) cells. Medium is 1592 SM buffer. Hosts: bacteria-free lysate, vertebrate cells, E.coli, E.coli RR1 pRK248 [ATCC 47036], E.coli Wgd5 [CRL 1817]. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
|GenBank:||M22642, M19164, M19165, M19166, M19167, M19168|