Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pCYM3
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 6543
- Notes
- The EcoRI, SmaI, and BamHI sites are 3' to the unique available in-phase initiation codon under control of the SV40 early promoter. Shuttle expression vector. (ATCC staff) Permits expression in both S. cerevisiae and mouse cells. (ATCC staff) Constructed by inserting a 1.207 kb ClaI/BamHI fragment containing URA3d into pCYM2 cut with BglII & ClaI (nt 3469 and 3589 according to the pCYM0 numbering scheme). URA3d is a modified URA3 gene which is not efficiently expressed and requires a large number of plasmids to complement a ura3 mutation. Restriction digests of the clone give the following sizes (kb): BglII--uncut; ClaI/BamHI--3.4,2.8,0.5; EcoRI--6.6; BamHI/PvuII--6.0, 0.5. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli CM5alpha, Saccharomyces cerevisiae, vertebrate cells, E.coli, Saccharomyces cerevisiae W303-1B/D, mouse C11D cell line. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 37826
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified