Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pFC54.t
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 5610
- Notes
- The clone contains a lambda pL promoter. [3] This was constructed from a SphI/XbaI fragment of pFC54 ligated to a 655 bp SphI/XbaI fragment of pHCW801 containing C-terminal 255 bp of Ser125 IL-2, 36 bp of 3' untranslated IL-2 cDNA and 394 bp of the positive retroregulatory element. [3] Construct contains a positive retroregulatory element which increases the level of production of the IL2 (Ser125) using a temperature regulated control cassette in a temperature-regulated ColE1 copy mutant plasmid vector. (personal communication) This should not be grown at temperatures exceeding 30C. The depositor recommends using ampicillin at 100 ug/ml. The strain is temperature inducible. (personal communication) Medium is 1315 1065 plus ampicillin (100 ug/ul). Hosts: E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 39789
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified