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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pKM2

Information

Plasmid Type
Unspecified
Cloning Method
Unknown
Size
4157
Notes
These data and their annotation were supplied to GenBank by Will Gilbert under the auspices of the GenBank Currator Program. Assembled from pKO2 and phage lambda by F. Pfeiffer. This is a promoter probe vector. The promoterless galK gene is used as selectable marker. Translationsal stop codons in all reading frames are present on a 11-bp oligonucleotide before the galKgene. Also, a polylinker was cloned before this gene. In addition, the lambda tr1 transcription terminator was inserted BEFORE the galK gene to allow measurement of very strong promoters due to read-through transcription. pKM2 does not contain the 4-bp deletion present in pKO2. However, in GenBank SYNPKO2 and SYNPKM2 have been mixed up. Thus, SYNPKO2 is given as a parent. Hosts: E.coli. Related vectors: pKM1, EcoGalK, pBR322, pKO-2, lambda. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
GenBank
L08922
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified

Sequence