Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pMT100
Information
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 1167
- Notes
- Dihydrofolate reductase (DHFR) is a highly conserved enzyme; the DHFRs encoded by the bacterial and animal genomes are similar to each other in size, amino acid sequence, and active site residues. In contrast, the plasmid-encoded type II DHFR and the bacterial chromosomal enzymes have different active sites and apparently distinct origins. Four BamHI linker insertion mutants were utilized as part of the sequencing strategy. Sites of insertion that destroyed the methotrexate resistance fell in two regions; one of these regions encodes a 78 amino acid polypeptide homologous to another drug-resistant DHFR, the second region essential for DHFR expression appears to be the promoter of the DHFR gene. The DHFR amino acid sequence derived from the DNA sequence is closely homologous to that of the R67 DHFR (see seperate entry), but differs in 17 of the 78 amino acid residues. NCBI gi: 207843 Hosts: E.coli. Related vectors: R388 from E. coli RR1. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified