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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pTF1

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
3043
Notes
pTF1 requires a reverse primer. This entry [NCBI gibbsq 122487] was created by the journal scanning component of NCBI/GenBank at the National Library of Medicine. This sequence comes from Fig. 1C. Deposited by: Allen Portner High-copy-number plasmid (in bacteria) with increased efficiency of liposome-mediated introduction into cultured cells. [1] Can express foreign sequences in cells producing T7 RNA polymerase, such as that encoded by recombinant vaccinia virus vTF7-3 ATCC VR-2153. [1] Constructed by inserting the 152 bp BamHI-BglII fragment from pAR2529 into the BamHI site of pTZ18R. Polylinker sites were removed and were replaced with the 48 bp EcoRI-KpnI fragment of pBluescript KS+. [1] The order of the major features of the plasmid is: universal primer sequence - XbaI - T7 phi 10 terminator < - XbaI - BamHI/MCS/EcoRI - < T7 phi 10 promoter - reverse primer sequence - pMB1 ori - < ampR - f1 ori.[1]CC A correction to the published sequence has been submitted. Sequence 2173-2252 should read: GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG. (personal communication) Restriction digests of the clone give the following sizes (kb): BamHI--3.0; EcoRI--3.0; HindIII--3.0. (ATCC staff) Medium is 1227 LB plus ampicillin. Sequence comes from Fig. 1C. [1] NCBI gi: 263065 Hosts: E.coli JM103, E.coli NM522, E.coli DH5alpha, vertebrate cells, HeLa T4+ cells. Related vectors: pTZ18R, pAR2529, pBluescript II KS+. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
77397
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified

Sequence