Luciferase vector. Cloning sites: BamHI, BglII, HindIII, KpnI, SalI, SmaI, SstI, XhoI. Restriction digests of the clone give the following sizes (kb): BamHI--6.1; HindIII--6.1; SalI--6.1; SmaI--6.1; XhoI--6.1. (ATCC staff) There is cryptic promoter activity which may be more readily observed with weak promoters. Be careful to do all appropriate controls and map the transcripts. (personal communication) This is a derivative of plasmids patented by the University of California and is not to be used for commercial purposes without prior arrangement with the University of California. (personal communication) One of a series of luciferase vectors (ATCC 37574-37584) for assaying promoter activity in mammalian cells. [1] Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, vertebrate cells, E.coli. Related vectors: pXPBg from pXPB from pSluc2 from pSV232AL-Adelta5', pXP1. (Information source: <a href=http://seq.yeastgenome.org/vectordb target=_blank>VectorDB</a>.)