Vector Database

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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

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Plasmid: pESC-LEU

Information

Plasmid Type

Yeast Expression

Promoter

Gal1 or Gal10

Expression Level

Unknown

Cloning Method

Restriction Enzyme

Size

7800

Selectable Marker

Leu2

Notes

The pESC vectors are a series of epitope-tagging vectors designed for expression and functional analysis of eukaryotic genes in the yeast S. cerevisiae. These vectors contain the GAL1 and GAL10 yeast promoters in opposing orientation. With these vectors one or two cloned genes can be introduced into a yeast host strain under the control of a repressible promoter. When two genes are co-expressed, protein-protein interactions can be confirmed by immunoprecipitation analysis.

Stable

Unspecified

Constitutive

Unspecified

Viral/Non-Viral

Nonviral

Sequence

Copy sequence

TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA 61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG 121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC 181 ACCATATCGA CTACGTCGTA AGGCCGTTTC TGACAGAGTA AAATTCTTGA GGGAACTTTC 241 ACCATTATGG GAAATGCTTC AAGAAGGTAT TGACTTAAAC TCCATCAAAT GGTCAGGTCA 301 TTGAGTGTTT TTTATTTGTT GTATTTTTTT TTTTTTAGAG AAAATCCTCC AATATCAAAT 361 TAGGAATCGT AGTTTCATGA TTTTCTGTTA CACCTAACTT TTTGTGTGGT GCCCTCCTCC 421 TTGTCAATAT TAATGTTAAA GTGCAATTCT TTTTCCTTAT CACGTTGAGC CATTAGTATC 481 AATTTGCTTA CCTGTATTCC TTTACTATCC TCCTTTTTCT CCTTCTTGAT AAATGTATGT 541 AGATTGCGTA TATAGTTTCG TCTACCCTAT GAACATATTC CATTTTGTAA TTTCGTGTCG 601 TTTCTATTAT GAATTTCATT TATAAAGTTT ATGTACAAAT ATCATAAAAA AAGAGAATCT 661 TTTTAAGCAA GGATTTTCTT AACTTCTTCG GCGACAGCAT CACCGACTTC GGTGGTACTG 721 TTGGAACCAC CTAAATCACC AGTTCTGATA CCTGCATCCA AAACCTTTTT AACTGCATCT 781 TCAATGGCCT TACCTTCTTC AGGCAAGTTC AATGACAATT TCAACATCAT TGCAGCAGAC 841 AAGATAGTGG CGATAGGGTC AACCTTATTC TTTGGCAAAT CTGGAGCAGA ACCGTGGCAT 901 GGTTCGTACA AACCAAATGC GGTGTTCTTG TCTGGCAAAG AGGCCAAGGA CGCAGATGGC 961 AACAAACCCA AGGAACCTGG GATAACGGAG GCTTCATCGG AGATGATATC ACCAAACATG 1021 TTGCTGGTGA TTATAATACC ATTTAGGTGG GTTGGGTTCT TAACTAGGAT CATGGCGGCA 1081 GAATCAATCA ATTGATGTTG AACCTTCAAT GTAGGGAATT CGTTCTTGAT GGTTTCCTCC 1141 ACAGTTTTTC TCCATAATCT TGAAGAGGCC AAAAGATTAG CTTTATCCAA GGACCAAATA 1201 GGCAATGGTG GCTCATGTTG TAGGGCCATG AAAGCGGCCA TTCTTGTGAT TCTTTGCACT 1261 TCTGGAACGG TGTATTGTTC ACTATCCCAA GCGACACCAT CACCATCGTC TTCCTTTCTC 1321 TTACCAAAGT AAATACCTCC CACTAATTCT CTGACAACAA CGAAGTCAGT ACCTTTAGCA 1381 AATTGTGGCT TGATTGGAGA TAAGTCTAAA AGAGAGTCGG ATGCAAAGTT ACATGGTCTT 1441 AAGTTGGCGT ACAATTGAAG TTCTTTACGG ATTTTTAGTA AACCTTGTTC AGGTCTAACA 1501 CTACCGGTAC CCCATTTAGG ACCAGCCACA GCACCTAACA AAACGGCATC AACCTTCTTG 1561 GAGGCTTCCA GCGCCTCATC TGGAAGTGGG ACACCTGTAG CATCGATAGC AGCACCACCA 1621 ATTAAATGAT TTTCGAAATC GAACTTGACA TTGGAACGAA CATCAGAAAT AGCTTTAAGA 1681 ACCTTAATGG CTTCGGCTGT GATTTCTTGA CCAACGTGGT CACCTGGCAA AACGACGATC 1741 TTCTTAGGGG CAGACATAGG GGCAGACATT AGAATGGTAT ATCCTTGAAA TATATATATA 1801 TATTGCTGAA ATGTAAAAGG TAAGAAAAGT TAGAAAGTAA GACGATTGCT AACCACCTAT 1861 TGGAAAAAAC AATAGGTCCT TAAATAATAT TGTCAACTTC AAGTATTGTG ATGCAAGCAT 1921 TTAGTCATGA ACGCTTCTCT ATTCTATATG AAAAGCCGGT TCCGGCCTCT CACCTTTCCT 1981 TTTTCTCCCA ATTTTTCAGT TGAAAAAGGT ATATGCGTCA GGCGACCTCT GAAATTAACA 2041 AAAAATTTCC AGTCATCGAA TTTGATTCTG TGCGATAGCG CCCCTGTGTG TTCTCGTTAT 2101 GTTGAGGAAA AAAATAATGG TTGCTAAGAG ATTCGAACTC TTGCATCTTA CGATACCTGA 2161 GTATTCCCAC AGTTAACTGC GGTCAAGATA TTTCTTGAAT CAGGCGCCTT AGACCGCTCG 2221 GCCAAACAAC CAATTACTTG TTGAGAAATA GAGTATAATT ATCCTATAAA TATAACGTTT 2281 TTGAACACAC ATGAACAAGG AAGTACAGGA CAATTGATTT TGAAGAGAAT GTGGATTTTG 2341 ATGTAATTGT TGGGATTCCA TTTTTAATAA GGCAATAATA TTAGGTATGT GGATATACTA 2401 GAAGTTCTCC TCGACCGTCG ATATGCGGTG TGAAATACCG CACAGATGCG TAAGGAGAAA 2461 ATACCGCATC AGGAAATTGT AAACGTTAAT ATTTTGTTAA AATTCGCGTT AAATTTTTGT 2521 TAAATCAGCT CATTTTTTAA CCAATAGGCC GAAATCGGCA AAATCCCTTA TAAATCAAAA 2581 GAATAGACCG AGATAGGGTT GAGTGTTGTT CCAGTTTGGA ACAAGAGTCC ACTATTAAAG 2641 AACGTGGACT CCAACGTCAA AGGGCGAAAA ACCGTCTATC AGGGCGATGG CCCACTACGT 2701 GAACCATCAC CCTAATCAAG TTTTTTGGGG TCGAGGTGCC GTAAAGCACT AAATCGGAAC 2761 CCTAAAGGGA GCCCCCGATT TAGAGCTTGA CGGGGAAAGC CGGCGAACGT GGCGAGAAAG 2821 GAAGGGAAGA AAGCGAAAGG AGCGGGCGCT AGGGCGCTGG CAAGTGTAGC GGTCACGCTG 2881 CGCGTAACCA CCACACCCGC CGCGCTTAAT GCGCCGCTAC AGGGCGCGTC GCGCCATTCG 2941 CCATTCAGGC TGCGCAACTG TTGGGAAGGG CGATCGGTGC GGGCCTCTTC GCTATTACGC 3001 CAGCTGAATT GGAGCGACCT CATGCTATAC CTGAGAAAGC AACCTGACCT ACAGGAAAGA 3061 GTTACTCAAG AATAAGAATT TTCGTTTTAA AACCTAAGAG TCACTTTAAA ATTTGTATAC 3121 ACTTATTTTT TTTATAACTT ATTTAATAAT AAAAATCATA AATCATAAGA AATTCGCTTA 3181 TTTAGAAGTG TCAACAACGT ATCTACCAAC GATTTGACCC TTTTCCATCT TTTCGTAAAT 3241 TTCTGGCAAG GTAGACAAGC CGACAACCTT GATTGGAGAC TTGACCAAAC CTCTGGCGAA 3301 GAATTGTTAA TTAAGAGCTC AGATCTTATC GTCGTCATCC TTGTAATCCA TCGATACTAG 3361 TGCGGCCGCC CTTTAGTGAG GGTTGAATTC GAATTTTCAA AAATTCTTAC TTTTTTTTTG 3421 GATGGACGCA AAGAAGTTTA ATAATCATAT TACATGGCAT TACCACCATA TACATATCCA 3481 TATACATATC CATATCTAAT CTTACTTATA TGTTGTGGAA ATGTAAAGAG CCCCATTATC 3541 TTAGCCTAAA AAAACCTTCT CTTTGGAACT TTCAGTAATA CGCTTAACTG CTCATTGCTA 3601 TATTGAAGTA CGGATTAGAA GCCGCCGAGC GGGTGACAGC CCTCCGAAGG AAGACTCTCC 3661 TCCGTGCGTC CTCGTCTTCA CCGGTCGCGT TCCTGAAACG CAGATGTGCC TCGCGCCGCA 3721 CTGCTCCGAA CAATAAAGAT TCTACAATAC TAGCTTTTAT GGTTATGAAG AGGAAAAATT 3781 GGCAGTAACC TGGCCCCACA AACCTTCAAA TGAACGAATC AAATTAACAA CCATAGGATG 3841 ATAATGCGAT TAGTTTTTTA GCCTTATTTC TGGGGTAATT AATCAGCGAA GCGATGATTT 3901 TTGATCTATT AACAGATATA TAAATGCAAA AACTGCATAA CCACTTTAAC TAATACTTTC 3961 AACATTTTCG GTTTGTATTA CTTCTTATTC AAATGTAATA AAAGTATCAA CAAAAAATTG 4021 TTAATATACC TCTATACTTT AACGTCAAGG AGAAAAAACC CCGGATCCGT AATACGACTC 4081 ACTATAGGGC CCGGGCGTCG ACATGGAACA GAAGTTGATT TCCGAAGAAG ACCTCGAGTA 4141 AGCTTGGTAC CGCGGCTAGC TAAGATCCGC TCTAACCGAA AAGGAAGGAG TTAGACAACC 4201 TGAAGTCTAG GTCCCTATTT ATTTTTTTAT AGTTATGTTA GTATTAAGAA CGTTATTTAT 4261 ATTTCAAATT TTTCTTTTTT TTCTGTACAG ACGCGTGTAC GCATGTAACA TTATACTGAA 4321 AACCTTGCTT GAGAAGGTTT TGGGACGCTC GAAGATCCAG CTGCATTAAT GAATCGGCCA 4381 ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC 4441 GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 4501 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 4561 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 4621 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 4681 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 4741 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 4801 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 4861 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 4921 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 4981 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC 5041 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 5101 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 5161 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 5221 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 5281 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 5341 AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT 5401 ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG 5461 CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA 5521 TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT 5581 ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT 5641 TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT 5701 TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT 5761 GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC 5821 CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC 5881 CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT 5941 GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG 6001 AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT 6061 ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC 6121 TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA 6181 GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG 6241 AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA 6301 TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGAAC GAAGCATCTG 6361 TGCTTCATTT TGTAGAACAA AAATGCAACG CGAGAGCGCT AATTTTTCAA ACAAAGAATC 6421 TGAGCTGCAT TTTTACAGAA CAGAAATGCA ACGCGAAAGC GCTATTTTAC CAACGAAGAA 6481 TCTGTGCTTC ATTTTTGTAA AACAAAAATG CAACGCGAGA GCGCTAATTT TTCAAACAAA 6541 GAATCTGAGC TGCATTTTTA CAGAACAGAA ATGCAACGCG AGAGCGCTAT TTTACCAACA 6601 AAGAATCTAT ACTTCTTTTT TGTTCTACAA AAATGCATCC CGAGAGCGCT ATTTTTCTAA 6661 CAAAGCATCT TAGATTACTT TTTTTCTCCT TTGTGCGCTC TATAATGCAG TCTCTTGATA 6721 ACTTTTTGCA CTGTAGGTCC GTTAAGGTTA GAAGAAGGCT ACTTTGGTGT CTATTTTCTC 6781 TTCCATAAAA AAAGCCTGAC TCCACTTCCC GCGTTTACTG ATTACTAGCG AAGCTGCGGG 6841 TGCATTTTTT CAAGATAAAG GCATCCCCGA TTATATTCTA TACCGATGTG GATTGCGCAT 6901 ACTTTGTGAA CAGAAAGTGA TAGCGTTGAT GATTCTTCAT TGGTCAGAAA ATTATGAACG 6961 GTTTCTTCTA TTTTGTCTCT ATATACTACG TATAGGAAAT GTTTACATTT TCGTATTGTT 7021 TTCGATTCAC TCTATGAATA GTTCTTACTA CAATTTTTTT GTCTAAAGAG TAATACTAGA 7081 GATAAACATA AAAAATGTAG AGGTCGAGTT TAGATGCAAG TTCAAGGAGC GAAAGGTGGA 7141 TGGGTAGGTT ATATAGGGAT ATAGCACAGA GATATATAGC AAAGAGATAC TTTTGAGCAA 7201 TGTTTGTGGA AGCGGTATTC GCAATATTTT AGTAGCTCGT TACAGTCCGG TGCGTTTTTG 7261 GTTTTTTGAA AGTGCGTCTT CAGAGCGCTT TTGGTTTTCA AAAGCGCTCT GAAGTTCCTA 7321 TACTTTCTAG AGAATAGGAA CTTCGGAATA GGAACTTCAA AGCGTTTCCG AAAACGAGCG 7381 CTTCCGAAAA TGCAACGCGA GCTGCGCACA TACAGCTCAC TGTTCACGTC GCACCTATAT 7441 CTGCGTGTTG CCTGTATATA TATATACATG AGAAGAACGG CATAGTGCGT GTTTATGCTT 7501 AAATGCGTAC TTATATGCGT CTATTTATGT AGGATGAAAG GTAGTCTAGT ACCTCCTGTG 7561 ATATTATCCC ATTCCATGCG GGGTATCGTA TGCTTCCTTC AGCACTACCC TTTAGCTGTT 7621 CTATATGCTG CCACTCCTCA ATTGGATTAG TCTCATCCTT CAATGCTATC ATTTCCTTTG 7681 ATATTGGATC ATACTAAGAA ACCATTATTA TCATGACATT AACCTATAAA AATAGGCGTA 7741 TCACGAGGCC CTTTCGTC