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viral-icon-01.png Viral Service: Lentivirus


Addgene's lentiviruses are made from plasmids that are currently in our repository. You can obtain the plasmid from us or receive a ready-to-use viral preparation from the inventory below.

Lentiviruses are distributed as purified viral particles in solution that are suitable for in vitro use. Volume and minimum titers are listed on each item's material page. Actual titers are reported with each shipment.

For more details about our viral service, visit our Viral Service landing page.

Browse Available Lentivirus

Screen.png Pooled CRISPR Libraries for Screening

Use pooled CRISPR libraries to screen for genes involved in specific biological processes. For more information, see our Guide to Plasmid Pooled Libraries.

ID Name Description Genes/Insert Selection PI
Human knockout pooled libraries
73178 Human sgRNA library Brunello in lentiGuide-Puro Human sgRNA library in backbone lentiGuide-Puro targeting 19,114 genes and containing 76,441 unique sgRNAs along with 1000 non-targeting controls. This backbone does not contain SpCas9. 76,441 unique sgRNAs targeting 19,114 human genes along with 1000 non-targeting controls Puromycin Doench, Root
73179 Human gRNA pooled library in lentiCRISPRv2 Human sgRNA library in backbone lentiCRISPRv2 targeting 19,114 genes and containing 76,441 unique sgRNAs along with 1000 non-targeting controls. This backbone contains SpCas9. SpCas9 and 76,441 unique sgRNAs targeting 19,114 human genes along with 1000 non-targeting controls Puromycin Doench, Root
Human activation pooled libraries
92379 Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A Human CRISPRa sgRNA activation library in backbone XPR_502 (P65 HSF) targeting 18,885 genes and containing 56,762 unique sgRNAs. This backbone does not contain dCas9. 56,762 unique sgRNAs targeting 18,885 human genes Puromycin Doench, Root
Mouse knockout pooled libraries
73633 Mouse sgRNA library Brie in lentiGuide-Puro Mouse sgRNA library in backbone lentiGuide-Puro containing 78,637 unique sgRNAs targeting 19,674 genes along with 1000 non-targeting controls. This backbone does not contain SpCas9. 78,637 unique sgRNAs targeting 19,674 mouse genes along with 1000 non-targeting controls Puromycin Doench, Root

Cut.png Cas9 Lentivirus

Cas9 viruses can be used to interrogate the genome in a variety of ways. Cas9 proteins can be guided to specific genomic sites by an RNA guide (gRNA) that harbors targeting sequences. Browse our repository for a list of validated gRNA plasmids. For more information on CRISPR plasmids and function, see our CRISPR plasmids resource page.

Browse our list of available Cas9 viruses by function:

Cut.png  Cas9 for Editing Genomic Sequences

To perform genome edits, Cas9 nuclease is used to generate double-stranded breaks (DSBs or cuts) or single-stranded breaks (nicks).

ID Name Insert Function Antibiotic Resistance Activity PI
52962 lentiCas9-Blast Sp Cas9 Cut Blasticidin Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. Lentiviral backbone. Zhang

Activate.png  Cas9 and Accessories for Activating Gene Expression

Catalytically-dead Cas9 (dCas9) can be fused to a transactivator and used as a transcriptional activator.

ID Name Insert Antibiotic Resistance Activity PI
61422 dCAS9-VP64_GFP dCAS9 (D10A, H840A) none Expresses dCAS9-VP64 activator with 2A GFP Zhang
61425 lenti dCAS-VP64_Blast dCAS9 (D10A, N863A) Blasticidin 3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE) Zhang
61426 lenti MS2-P65-HSF1_Hygro MS2-P65-HSF1 Hygromycin lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker (EF1a-MS2-p65-HSF1-2A-Hygro-WPRE) Zhang

Epigenetics.png  Cas9 for Editing DNA Methylation

Fusion of catalytically-dead Cas9 (dCas9) to epigenetic modifiers enables targeted DNA methylation editing. By the addition or removal of methyl groups to a promoter sequence, gene expression can be silenced or activated, respectively.

ID Name Antibiotic Resistance Activity PI
84476 Fuw-dCas9-Dnmt3a Zeocin dCas9 fused with Dnmt3a, which induces de novo methylation Jaenisch
84478 Fuw-dCas9-Dnmt3a_IM Zeocin dCas9 fused with the inactive Dnmt3a catalytic domain (E664A, E756A) Jaenisch
84475 Fuw-dCas9-Tet1CD Zeocin dCas9 fused with the catalytic domain of Tet1, which induces demethylation Jaenisch
84479 Fuw-dCas9-Tet1CD_IM Zeocin dCas9 fused with an inactive catalytic domain of Tet1 (H1672Y, D1674A) Jaenisch

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lentiviral-icon.png Non-CRISPR Lentivirus

Browse our list of available viruses based on plasmids in our repository that are not used for CRISPR.

ID Name Insert Antibiotic Resistance Purpose PI
17446 pLenti CMV GFP Hygro (656-4) GFP Hygromycin 3rd gen lentiviral eGFP expression vector, CMV promoter, Hygro Campeau

Screen.png Pooled Barcoding Libraries

ID Name Description PI
115643 Morris CellTag Barcode Library Version 1 Ready-to-use lentiviral particles carrying version 1 of the CellTag barcoding library. Version 1 of the Celltag library contains 19973 barcodes to combinatorially index cells for single-cell analysis of clonal dynamics. This library expresses EGFP for easy visualization via direct fluorescence. Morris
115644 Morris CellTag Barcode Library Version 2 Ready-to-use lentiviral particles carrying version 2 of the CellTag barcoding library. Version 2 of the Celltag library contains 4934 barcodes to combinatorially index cells for single-cell analysis of clonal dynamics. This library expresses EGFP for easy visualization via direct fluorescence. Morris
115645 Morris CellTag Barcode Library Version 3 Ready-to-use lentiviral particles carrying version 3 of the CellTag barcoding library. Version 3 of the Celltag library contains 5737 barcodes to combinatorially index cells for single-cell analysis of clonal dynamics. This library expresses EGFP for easy visualization via direct fluorescence. Morris

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Don’t See What You’re Looking For?

Please let us know what you would like to see available in viral format using the form below. Please note this does not guarantee viral service, but lets us know what viruses would be helpful to the scientific community.