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viral-icon-01.png Viral Service: Lentivirus


We are offering ready-to-use viral preparations of several popular lentiviral plasmids and pooled libraries in our repository. With viral service, Addgene provides high-quality and high-titer viral preparations. We perform quality control experiments, including using our lentiviruses for stable cell line generation, colony formation titering assay, or fluorescence titering assay. You can see the results of our quality control experiments on the material pages for viral catalog items. For descriptions of Addgene's viral production protocols and quality controls, visit our viral production page. For protocols on how to use virus from Addgene or how to make your own virus, see our list of viral protocols with tips and sample data.

Addgene's lentiviruses are made from plasmids that are currently in our repository, so feel free to obtain the plasmid from us, or take advantage of our viral service by browsing our available lentiviruses below!


Browse Available Lentivirus by Function

Lentiviral Preparations of Pooled CRISPR Libraries for Screening

Use pooled CRISPR libraries to screen for genes involved in specific biological processes. For more information, see our Guide to Plasmid Pooled Libraries.

ID Name Description Genes/Insert Selection PI
73178 Human sgRNA library Brunello in lentiGuide-Puro Human sgRNA library in backbone lentiGuide-Puro targeting 19,114 genes and containing 76,441 unique sgRNAs along with 1000 non-targeting controls 76,441 unique sgRNAs targeting 19,114 human genes along with 1000 non-targeting controls Puromycin Root
73633 Mouse sgRNA library Brie in lentiGuide-Puro Mouse sgRNA library in backbone lentiGuide-Puro containing 78,637 unique sgRNAs targeting 19,674 genes along with 1000 non-targeting controls 78,637 unique sgRNAs targeting 19,674 mouse genes along with 1000 non-targeting controls Puromycin Root

Cas9 and CRISPR-activator Lentivirus

Cas9 viruses can be used to interrogate the genome in a variety of ways. To perform genome edits, Cas9 nuclease is used to generate double-stranded breaks (DSBs or cuts) or single-stranded breaks (nicks). When fused to a transcriptional repressor, catalytically-dead Cas9 mutants (dCas9) can interfere with and reduce transcription. Alternatively, dCas9 can be fused to a transactivator and used as a transcriptional activator. Finally, when fused to a fluorescent protein, dCas9 can be used to visualize genomic loci.

Browse our list of available Cas9 and CRISPR-activator lentiviruses based on their functions. Cas9 proteins can be guided to specific genomic sites by an RNA guide (gRNA) that harbors targeting sequences. See our list of experimentally validated gRNA viruses in viral format below or browse our repository for a list of validated gRNA plasmids. For more information on CRISPR plasmids and function, see our CRISPR plasmids resource page.

ID Name Insert Function Antibiotic Resistance Purpose PI
52962 lentiCas9-Blast Sp Cas9 Cut Blasticidin Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. Lentiviral backbone. Zhang
63593 lentiCas9n (D10A)-Blast Sp Cas9 Nick Blasticidin Expresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone. Zhang
50917 pHAGE TRE dCas9-KRAB dCas9 Interfere Neomycin (select with G418) Tet-regulatable dCas9-KRAB lentiviral expression vector Maehr, Wolfe
50919 pHAGE EF1α dCas9-KRAB dCas9 Interfere Puromycin Constitutive dCas9-KRAB lentiviral expression vector Maehr, Wolfe
61422 dCAS9-VP64_GFP dCAS9 (D10A, H840A) Activate none Expresses dCAS9-VP64 activator with 2A GFP Zhang
61425 lenti dCAS-VP64_Blast dCAS9 (D10A, N863A) Activate Blasticidin 3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE) Zhang
61426 lenti MS2-P65-HSF1_Hygro MS2-P65-HSF1 Activate Hygromycin lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker (EF1a-MS2-p65-HSF1-2A-Hygro-WPRE) Zhang
64107 pHAGE-TO-dCas9-3XGFP Sp dCas9 Visualize none Expression of Sp dCas9-3XGFP in mammalian cells Pederson
64108 pHAGE-TO-dCas9-3XmCherry Sp dCas9 Visualize none Expression of Sp dCas9-3XmCherry in mammalian cells Pederson

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gRNA Lentivirus

Browse our list of experimentally validated gRNA viruses to target specific genes. These viruses are for use in Cas9-expressing cells. For more gRNA plasmids, browse our list of validated gRNA plasmids and use our protocols to generate lentivirus.

ID Name Target Antibiotic Resistance Purpose PI
59702 pXPR_011 EGFP Puromycin This lentiviral vector can be used to assay Cas9 activity Doench, Root
76479 BRAF gRNA (BRDN0001145345) BRAF Puromycin 3rd generation lentiviral gRNA plasmid targeting human BRAF Doench, Root
76480 BRAF gRNA (BRDN0001146266) BRAF Puromycin 3rd generation lentiviral gRNA plasmid targeting human BRAF Doench, Root
76510 MAP3K4 gRNA (BRDN0001148639) MAP3K4 Puromycin 3rd generation lentiviral gRNA plasmid targeting human MAP3K4 Doench, Root
80034 EGFP gRNA (BRDN0000561167) EGFP Puromycin 3rd generation lentiviral gRNA plasmid targeting EGFP Doench, Root
80035 EGFP gRNA (BRDN0000562805) EGFP Puromycin 3rd generation lentiviral gRNA plasmid targeting EGFP Doench, Root
80036 EGFP gRNA (BRDN0000563266) EGFP Puromycin 3rd generation lentiviral gRNA plasmid targeting EGFP Doench, Root

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Non-CRISPR Lentivirus

Browse our list of available viruses based on plasmids in our repository that are not used for CRISPR.

ID Name Insert Antibiotic Resistance Purpose PI
1864 scramble shRNA scramble Puromycin 3rd gen lentiviral negative control vector containing scrambled shRNA Sabatini
12265 pHR-CMV-nlsCRE Cre none CMV-drive expression of Cre recombinase Trono
17408 Puro.Cre empty vector Cre Puromycin Empty backbone expressing Cre recombinase and pyromycin resistance Jacks
17446 pLenti CMV GFP Hygro (656-4) GFP Hygromycin 3rd gen lentiviral eGFP expression vector, CMV promoter, Hygro Campeau
26429 pLenti CMV rtTA3 Blast (w756-1) Tetracycline repressor A3 mutant Blasticidin 3rd gen lentiviral reverse tetracycline-controlled transactivator 3 (rtTA3) expression vector, CMV promoter, Blast Campeau
59700 pRosetta EGFP Puromycin, Blasticidin Lentiviral vector for use as a standard for titering experiments when trying to compare vectors with different selection markers or to determine appropriate antibiotic conditions Doench, Root
74218 pLenti puro HA-Ubiquitin ubiquitin Puromycin General lentiviral expression plasmid Fan

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Don’t See What You’re Looking For?

Please let us know what you would like to see available in viral format using the form below. Please note this does not guarantee viral service, but lets us know what viruses would be helpful to the scientific community.