Vaccinia Virus ORF Library
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This is a collection of open reading frames from the Vaccinia Virus genome, made by Jack Bennink and Jon Yewdell’s laboratories at the NIH. You may Browse clones in the library.
ORF Information
This library is described in
Identification of poxvirus CD8+ T cell determinants to enable rational design and characterization of smallpox vaccines. Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW. J Exp Med. 2005 Jan 3. 201(1):95-104. (PubMed).
The plasmids are divided into 3 plates: Plate 1, Plate 2, Plate 3.
Also, see:
Notes from author
Tab delimited summary of clones
Please note that vectors in this library have not been completely sequenced.
Pricing and Distribution
Individual Plasmids: Individual plasmids can be ordered at $65 per plasmid (with discounts for bulk orders), and will be shipped as bacterial stabs.
The Entire Kit (cat#1000000009): This library is $800, plus shipping and handling. This kit will be shipped as bacterial glycerol stocks, 96-well format, on dry ice. Samples should be frozen at -80oC immediately upon arrival.
Note that Addgene uses UPS for shipments and can ship dry ice to most countries, excluding China. Scientists from China interested in obtaining this kit should contact help@addgene.org.