pCC25
(Plasmid
#177319)
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PurposeExpression plasmid for production of recombinant T. terrestris CutA NTase fragment in E. coli
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 177319 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET28
- Backbone size w/o insert (bp) 5539
- Total vector size (bp) 6634
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameTtCutA 240-603
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SpeciesThielavia terrestris
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Insert Size (bp)1095
- Promoter T7
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Tag
/ Fusion Protein
- 6xHis-SUMOTag (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7terminator (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCC25 was a gift from Rafal Tomecki (Addgene plasmid # 177319 ; http://n2t.net/addgene:177319 ; RRID:Addgene_177319) -
For your References section:
Reproducible and efficient new method of RNA 3'-end labelling by CutA nucleotidyltransferase-mediated CC-tailing. Tomecki R, Kobylecki K, Drazkowska K, Hyjek-Skladanowska M, Dziembowski A. RNA Biol. 2021 Nov 12;18(sup2):623-639. doi: 10.1080/15476286.2021.1999104. Epub 2021 Nov 12. 10.1080/15476286.2021.1999104 PubMed 34766865
