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Addgene

VKG1-gRNA-pX330
(Plasmid #108671)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 108671 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pX330
  • Modifications to backbone
    VKG1-gRNA 5'-AACGACCGAGCGCAGCGA-3' was cloned into pX330
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    gRNA for VKG1 sequence
  • gRNA/shRNA sequence
    VKG1
  • Species
    Synthetic

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    VKG1-gRNA-pX330 was a gift from Shigeo Sugano (Addgene plasmid # 108671 ; http://n2t.net/addgene:108671 ; RRID:Addgene_108671)
  • For your References section:

    Development of versatile non-homologous end joining-based knock-in module for genome editing. Sawatsubashi S, Joko Y, Fukumoto S, Matsumoto T, Sugano SS. Sci Rep. 2018 Jan 12;8(1):593. doi: 10.1038/s41598-017-18911-9. 10.1038/s41598-017-18911-9 [pii] PubMed 29330493