ATG-3925 (CBG2)
(Plasmid #167839)

• Purpose: Expresses CBG2 (Click Beetle Green 2) in E. coli and mammalian cells
• Depositing Lab: Lance Encell
• Publication: Zambito et al iScience. 2020 Dec 26;24(1):101986. doi: 10.1016/j.isci.2020.101986. eCollection 2021 Jan 22.

Backbone

• Vector backbone: pF4Ag
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 3820
• Total vector size (bp): 5470
• Vector type: Mammalian Expression, Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Use KRX bacterial cells for protein expression. Induced by Rhamnose with 25C.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Click Beetle Green 2
• Alt name: CBG-2
• Species: Click Beetle
• Insert Size (bp): 1650
• Mutation: Q348H. Click Beetle Green 2 (CBR2) has 3 amino acid changes compared to Click Beetle Red 2 (CBR2).
• Promoter: CMV, T7
• Tag / Fusion Protein:
  • 6-His (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Sgf1 (not destroyed)
• 3′ cloning site: Pme1 (not destroyed)
• 5′ sequencing primer: T7 promoter (TAA TAC GAC TCA CTA TAG GG)
• 3′ sequencing primer: T7 terminator (GCT AGT TAT TGC TCA GCG G)

Resource Information

• Supplemental Documents:
  • ATG-3925.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • CBG2 Limited Use Label License
• Industry Terms:
  • Not Available to Industry