pIVEX2.3-Theo-3c-EGFP
(Plasmid
#217526)
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PurposeTheo-3c riboswitch-regulated GFP reporter. The gene is inserted into downstream of T7 promoter.
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 217526 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepIVEX2.3
- Total vector size (bp) 4236
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Vector typeIn vitro transcription-translation
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTheo-3c riboswitch-regulated EGFP
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SpeciesSynthetic
- Promoter T7
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer ATGCGTCCGGCGTAGAGGATCGAGA
- 3′ sequencing primer AGGGGTTATGCTAGTTATTGCTCAGCGG (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pIVEX2.3-Theo-3c-EGFP was a gift from Keisuke Fukunaga (Addgene plasmid # 217526 ; http://n2t.net/addgene:217526 ; RRID:Addgene_217526) -
For your References section:
Switchable and orthogonal gene expression control inside artificial cells by synthetic riboswitches. Ishii Y, Fukunaga K, Cooney A, Yokobayashi Y, Matsuura T. Chem. Commun., 2024 10.1039/D4CC00965G
