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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 22091 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBluescript
- Backbone size w/o insert (bp) 4000
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSprouty 1
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SpeciesM. musculus (mouse)
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Insert Size (bp)1500
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Entrez GeneSpry1 (a.k.a. sprouty1, spry-1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Mouse Sprouty1 cDNA clone was isolated from Lambda Zap mouse cDNA library. The insert contains the complete coding region as well as some 5' and 3' UTR sequences. The 4kb vector is presumed to be pBluescript with extra sequences from Lambda Zap plasmid.
For antisense probe, linearize the plasmid with EcoRI and use T7 Polymerase. The transcript should be approximately 1.5kb.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mSprouty1/3B14 was a gift from Gail Martin (Addgene plasmid # 22091 ; http://n2t.net/addgene:22091 ; RRID:Addgene_22091) -
For your References section:
Vertebrate Sprouty genes are induced by FGF signaling and can cause chondrodysplasia when overexpressed. Minowada G, Jarvis LA, Chi CL, Neubuser A, Sun X, Hacohen N, Krasnow MA, Martin GR. Development. 1999 Oct . 126(20):4465-75. PubMed 10498682
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/77/89/f5d860c8-af62-11e0-90fe-003048dd6500.jpeg.940x940_q85_autocrop.png)