pMLS7
(Plasmid
#32056)
-
Purpose(Empty Backbone) Vector optimized for constitutive gene expression in Burkholderia genus and related bacteria
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 32056 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepME6000
-
Vector typeBacterial Expression
- Promoter Ps7 promoter, originated from Paraburkholderia xenovorans LB400
Growth in Bacteria
-
Bacterial Resistance(s)Trimethoprim
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberLow Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer AGCTGGCAACCAATTAGC
- 3′ sequencing primer pBAD reverse (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pMLS7 was a gift from Miguel Valvano (Addgene plasmid # 32056 ; http://n2t.net/addgene:32056 ; RRID:Addgene_32056) -
For your References section:
Construction and evaluation of plasmid vectors optimized for constitutive and regulated gene expression in Burkholderia cepacia complex isolates. Lefebre MD, Valvano MA. Appl Environ Microbiol. 2002 Dec;68(12):5956-64. 10.1128/AEM.68.12.5956-5964.2002 PubMed 12450816
