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Showing: 101 - 120 of 1880 results

  3. pME-Cas9-T2A-GFP

    Plasmid
    #63155
    Purpose
    Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS and followed by inframe GFP. Cas9 and GFP are separated by the sequence of a T2A self-cleaving peptide.
    Depositor
    Leonard Zon
    Article
    Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15
    Insert
    Cas9-T2A-GFP
    Use
    CRISPR; Tol2 middle entry vector for gateway
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    63155-plasmid-map-sequence-id-220487

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  6. pDONR221-nrip1b

    Plasmid
    #192732
    Purpose
    Gateway entry vector encoding zebrafish nrip1b
    Depositor
    Summer Thyme
    Insert
    nrip1b (nrip1b Zebrafish)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    L661P (rs511527097); S720P; A801T; K862E; A863P; …
    Promoter
    None
    Availability
    Academic Institutions and Nonprofits only
    192732-plasmid-map-sequence-id-377800

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  7. pDONR221-appa

    Plasmid
    #192745
    Purpose
    Gateway entry vector encoding zebrafish appa
    Depositor
    Summer Thyme
    Insert
    appa (appa Zebrafish)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    S47N
    Promoter
    None
    Availability
    Academic Institutions and Nonprofits only
    192745-plasmid-map-sequence-id-377796

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  8. pAWO-SUMO-3C_14M_zTDP2

    Plasmid
    #200512
    Purpose
    Expression of zebrafish TDP2 recombinant protein
    Depositor
    Antony Oliver
    Insert
    TDP2 (tdp2b Zebrafish)
    Use
    Tags
    HIS-SUMO
    Expression
    Bacterial
    Mutation
    L136N, A139S, C239T, K240R, K309Q, K314L, T315G, …
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    200512-plasmid-map-sequence-id-396440

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  9. pKHR7

    Plasmid
    #74624
    Purpose
    a cmlc2::mCherry (red heart) reporter gene was inserted at the 3’end of pKHR4 mcs, that resides within donor fragments produced by I-SceI digestion. Genbank accession number is KU144824.
    Depositor
    David Grunwald
    Article
    Hoshijima et al Dev Cell. 2016 Mar 21;36(6):654-67. doi:
    Insert
    cmlc2::mCherry
    Use
    Zebrafish expression
    Tags
    Expression
    Mutation
    Promoter
    cmlc2
    Availability
    Academic Institutions and Nonprofits only
    74624-plasmid-map-sequence-id-204495

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  19. pKHR5

    Plasmid
    #74593
    Purpose
    an alpha-crystallin::Venus (green lens) reporter cassette flanked by FRT sites and with a single loxP site at the 3’ end was inserted in the middle of pKHR4 mcs. Genbank accession number is KU144823.
    Depositor
    David Grunwald
    Article
    Hoshijima et al Dev Cell. 2016 Mar 21;36(6):654-67. doi:
    Insert
    FRT-alpha-crystallin::Venus-FRT-loxP reporter cassette
    Use
    Cre/Lox; Zebrafish expression
    Tags
    Expression
    Mutation
    Promoter
    alpha-crystallin
    Availability
    Academic Institutions and Nonprofits only
    74593-plasmid-map-sequence-id-140738

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  20. pKHR8

    Plasmid
    #74625
    Purpose
    a plasmid derived from pKHR5: in addition to Venus reporter gene, it also contains cmlc2::mCherry (red heart) reporter gene at 3’ end of the mcs as pKHR7. Genbank accession number is KU144825.
    Depositor
    David Grunwald
    Article
    Hoshijima et al Dev Cell. 2016 Mar 21;36(6):654-67. doi:
    Inserts
    FRT-alpha-crystallin::Venus-FRT-loxP reporter cassette
    cmlc2::mCherry
    Use
    Cre/Lox; Zebrafish expression
    Tags
    Expression
    Mutation
    Promoter
    alpha-crystallin and cmlc2
    Availability
    Academic Institutions and Nonprofits only
    74625-plasmid-map-sequence-id-200444

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Showing: 101 - 120 of 1880 results