6206 pETH6K218TK620Tb
(Plasmid
#1239)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 1239 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET28a
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHsp104 (K218T:K620T)
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Alt nameHsp104
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SpeciesS. cerevisiae (budding yeast)
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MutationK218TK620T Please see Depositor Comments
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Entrez GeneHSP104 (a.k.a. YLL026W)
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Tag
/ Fusion Protein
- His tag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site nde1 (not destroyed)
- 3′ cloning site sac1 (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byEric Schirmer
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Addgene NGS found an additional A315T mutation in the Hsp104 translation of unknown functional consequence
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
6206 pETH6K218TK620Tb was a gift from Susan Lindquist (Addgene plasmid # 1239) -
For your References section:
Hsp104 catalyzes formation and elimination of self-replicating Sup35 prion conformers. Shorter J, Lindquist S. Science 2004 Jun 18;304(5678):1793-7. Epub 2004 May 20. 10.1126/science.1098007 PubMed 15155912