pcDNA3.1 N-FLAG/Hyg(+)
(Plasmid
#15439)
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Purpose(Empty Backbone)
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Depositing Labs
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 15439 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepcDNA3.1 N-FLAG/Hygro
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Backbone manufacturerConaway Lab
- Backbone size (bp) 5600
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- FLAG (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer BGH rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pcDNA3.1/Hygro (from Invitrogen) was modified to include an N-terminal FLAG tag. See Author's Map for cloning details.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1 N-FLAG/Hyg(+) was a gift from Joan Conaway & Ronald Conaway (Addgene plasmid # 15439)
Map uploaded by the depositor.