Torso EcoRI-EcoRI Y657F in pBSK
(Plasmid
#39803)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 39803 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBlueScript
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 3000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTorso
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Alt nametor
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SpeciesD. melanogaster (fly)
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MutationY657F
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Entrez Genetor (a.k.a. Dmel_CG1389, CG1389, Dmel\CG1389, TOR, TORSO, Tor, Torso, dTor, splc)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Perrimon Lab plasmid #221
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Torso EcoRI-EcoRI Y657F in pBSK was a gift from Norbert Perrimon (Addgene plasmid # 39803) -
For your References section:
Synergistic activities of multiple phosphotyrosine residues mediate full signaling from the Drosophila Torso receptor tyrosine kinase. Gayko U, Cleghon V, Copeland T, Morrison DK, Perrimon N. Proc Natl Acad Sci U S A. 1999 Jan 19;96(2):523-8. 10.1073/pnas.96.2.523 PubMed 9892666