YCplac111::TOR1 (pTS19)
(Plasmid
#42904)
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Depositing Lab
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Publication
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Sequence Information
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Please contact us if you would like Addgene to sequence a portion of this plasmid before you request it.
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Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 42904 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backboneYCplac111
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Backbone manufacturerR.D. Gietz and A. Sugino
- Backbone size w/o insert (bp) 6112
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Vector typeYeast Expression
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTOR1
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SpeciesS. cerevisiae (budding yeast)
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Entrez GeneTOR1 (a.k.a. YJR066W, DRR1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SacI (unknown if destroyed)
- 3′ cloning site SacI (unknown if destroyed)
- 5′ sequencing primer M13pUC-fwd
- 3′ sequencing primer M13pUC-rev
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
TOR1 fragment (~13.5kb SacI fragment) from pPW20 cloned into YCplac111
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
YCplac111::TOR1 (pTS19) was a gift from Michael Hall (Addgene plasmid # 42904)
Map uploaded by the depositor.