pcDNA3.1-D2-YFP
(Plasmid
#44194)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 44194 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepcDNA3.1
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5428
- Total vector size (bp) 7373
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDopamine D2
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SpeciesR. norvegicus (rat)
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Insert Size (bp)1245
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GenBank IDM36831
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Entrez GeneDrd2
- Promoter CMV
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Tag
/ Fusion Protein
- YFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site EcoR1 (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer BGH primer (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1-D2-YFP was a gift from Narasimhan Gautam (Addgene plasmid # 44194) -
For your References section:
Shuttling of G protein subunits between the plasma membrane and intracellular membranes. Chisari M, Saini DK, Kalyanaraman V, Gautam N. J Biol Chem. 2007 Aug 17;282(33):24092-8. Epub 2007 Jun 18. 10.1074/jbc.M704246200 PubMed 17576765