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pcDNA3.1 Makona EBOV B10
(Plasmid #87820)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 87820 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Currently unavailable outside the U.S.

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 7031
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EBOV GP 'Makona' strain - B10
  • Species
    Virus
  • Insert Size (bp)
    2031
  • Mutation
    A82V P375S
  • GenBank ID
    AKG65286.1
  • Entrez Gene
    GP (a.k.a. ZEBOVgp4)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1 Makona EBOV B10 was a gift from Jonathan Ball (Addgene plasmid # 87820)
  • For your References section:

    Human Adaptation of Ebola Virus during the West African Outbreak. Urbanowicz RA, McClure CP, Sakuntabhai A, Sall AA, Kobinger G, Muller MA, Holmes EC, Rey FA, Simon-Loriere E, Ball JK. Cell. 2016 Nov 3;167(4):1079-1087.e5. doi: 10.1016/j.cell.2016.10.013. 10.1016/j.cell.2016.10.013 PubMed 27814505