pcDNA3.1 Makona EBOV B10
(Plasmid
#87820)
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PurposeEbola Virus glycoprotein (Makona-derived) in CMV IE driven mammalian expression vector
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 87820 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Currently unavailable outside the U.S.
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepcDNA3.1
- Backbone size w/o insert (bp) 5000
- Total vector size (bp) 7031
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEBOV GP 'Makona' strain - B10
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SpeciesVirus
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Insert Size (bp)2031
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MutationA82V P375S
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GenBank IDAKG65286.1
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Entrez GeneGP (a.k.a. ZEBOVgp4)
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer T7F
- 3′ sequencing primer bGHR (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1 Makona EBOV B10 was a gift from Jonathan Ball (Addgene plasmid # 87820) -
For your References section:
Human Adaptation of Ebola Virus during the West African Outbreak. Urbanowicz RA, McClure CP, Sakuntabhai A, Sall AA, Kobinger G, Muller MA, Holmes EC, Rey FA, Simon-Loriere E, Ball JK. Cell. 2016 Nov 3;167(4):1079-1087.e5. doi: 10.1016/j.cell.2016.10.013. 10.1016/j.cell.2016.10.013 PubMed 27814505