pYACHAC-no-centromere
(Plasmid
#89225)
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Purpose(Empty Backbone) pYACHAC minus the centromere
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 89225 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepYAC4
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Backbone manufacturerATCC
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Modifications to backboneAdded human insulators, GFP marker, blasticidin marker, LacI-CENP-A into
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Vector typeMammalian Expression, Yeast Expression
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Selectable markersBlasticidin, TRP1, URA3, HIS3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
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Addgene Notes
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A portion of this plasmid was derived from a plasmid made bySynthesized by Synthetic Genomics Inc
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYACHAC-no-centromere was a gift from Pamela Silver (Addgene plasmid # 89225)