pBAC02-KAN-Centromere
(Plasmid
#89227)
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PurposeThe de novo centromere in pBAC02-KAN assembled by SGI
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 89227 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBAC02-KAN
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Backbone manufacturerSynthetic Genomics Inc
- Backbone size w/o insert (bp) 7116
- Total vector size (bp) 12587
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameDe novo human centromere
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SpeciesH. sapiens (human)
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Insert Size (bp)5471
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GenBank IDNot applicable Not applicable
- Promoter None
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer Too repetitive to sequence normally
- 3′ sequencing primer Too repetitive to sequence normally (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Addgene Notes
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBAC02-KAN-Centromere was a gift from Pamela Silver (Addgene plasmid # 89227)