PurposeTo express GFP-tagged SAHS1 in mammalian cells transiently
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||90029||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
- Promoter CMV
/ Fusion Protein
- AcGFP1 (C terminal on insert)
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Insert species is Ramazzottius varieornatus
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAcGFP1-N1-SAHS1 was a gift from Takekazu Kunieda (Addgene plasmid # 90029)
For your References section:Two novel heat-soluble protein families abundantly expressed in an anhydrobiotic tardigrade. Yamaguchi A, Tanaka S, Yamaguchi S, Kuwahara H, Takamura C, Imajoh-Ohmi S, Horikawa DD, Toyoda A, Katayama T, Arakawa K, Fujiyama A, Kubo T, Kunieda T. PLoS One. 2012;7(8):e44209. DOI:10.1371/journal.pone.0044209 PubMed 22937162
Generated by Addgene from full sequence supplied by depositor.