pG1-TyDown (Trp)
(Plasmid
#90391)
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Purpose3'SS competition construct
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 90391 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepG1
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Vector typeYeast Expression
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Selectable markersTRP1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameACT1-CUP1
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SpeciesS. cerevisiae (budding yeast)
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Mutation3'SS competition construct
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Entrez GeneACT1 (a.k.a. YFL039C, ABY1, END7)
- Promoter pGPD overexpression promoter
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer pGPD pro (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pG1-TyDown (Trp) was a gift from Christine Guthrie (Addgene plasmid # 90391) -
For your References section:
A novel role for a U5 snRNP protein in 3' splice site selection. Umen JG, Guthrie C. Genes Dev. 1995 Apr 1;9(7):855-68. PubMed 7535718