pAAV-RAM-d2TTA::TRE-tdTomato-WPREpA
(Plasmid
#92183)
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PurposepAAV-RAM-tdTomato
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 92183 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneDerived from AAV V032
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Backbone manufacturerRachael Neve
- Total vector size (bp) 9084
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nametdTomato
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SpeciesSynthetic
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Insert Size (bp)1431
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MutationT230I (please see depositor comments below)
- Promoter TRE
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (not destroyed)
- 3′ cloning site AscI (not destroyed)
- 5′ sequencing primer NA (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bytdTomato; from plasmid pAAV-hSyn-iCre-dTomato (JT) provided by Dr. Jonathan Ting (MIT, now at Allen Institute)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that mutation T230I in TdTomato was found during Addgene's quality control. The depositor noted that this mutation does NOT affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-RAM-d2TTA::TRE-tdTomato-WPREpA was a gift from Yingxi Lin (Addgene plasmid # 92183) -
For your References section:
A robust activity marking system for exploring active neuronal ensembles. Sorensen AT, Cooper YA, Baratta MV, Weng FJ, Zhang Y, Ramamoorthi K, Fropf R, LaVerriere E, Xue J, Young A, Schneider C, Gotzsche CR, Hemberg M, Yin JC, Maier SF, Lin Y. Elife. 2016 Sep 23;5. pii: e13918. doi: 10.7554/eLife.13918. 10.7554/eLife.13918 PubMed 27661450