SARS-CoV-2 spike-protein D614G mutation increases virion spike density and infectivity.
Zhang L, Jackson CB, Mou H, Ojha A, Peng H, Quinlan BD, Rangarajan ES, Pan A, Vanderheiden A, Suthar MS, Li W, Izard T, Rader C, Farzan M, Choe H
Nat Commun. 2020 Nov 26;11(1):6013. doi: 10.1038/s41467-020-19808-4. PubMed Article
Zhang L, Jackson CB, Mou H, Ojha A, Peng H, Quinlan BD, Rangarajan ES, Pan A, Vanderheiden A, Suthar MS, Li W, Izard T, Rader C, Farzan M, Choe H
Nat Commun. 2020 Nov 26;11(1):6013. doi: 10.1038/s41467-020-19808-4. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 156418 | pCAGGS-SARS2-S-D614 (NC-flag) | Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at N- and C-termini. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors. |
| 156419 | pCAGGS-SARS2-S-G614 (NC-flag) | Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at N- and C-termini. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors. |
| 156420 | pCAGGS-SARS2-S-D614 (C-flag) | Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at C-terminus. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors. |
| 156421 | pCAGGS-SARS2-S-G614 (C-flag) | Mammalian expression of SARS-CoV-2 S protein (G614) with the flag tag at C-terminus. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors. |
| 159363 | pCAGGS-SARS2-S-FKO (NC-flag) | Mammalian expression of SARS-CoV-2 S protein (FKO: furin-cleavage site mutant) with the flag tag at N- and C-termini. |
| 159364 | pCAGGS-SARS2-S-FKO (C-flag) | Mammalian expression of SARS-CoV-2 S protein (FKO: furin-cleavage site mutant) with the flag tag at C-terminus. |